Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,074,770 G→T 7.7% intergenic (‑88/+53) ohrA ← / ← ohrR Organic hydroperoxide resistance protein OhrA/Organic hydroperoxide resistance transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,074,7700GT 7.7% 186.0 / 2.2 52intergenic (‑88/+53)ohrA/ohrROrganic hydroperoxide resistance protein OhrA/Organic hydroperoxide resistance transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (31/17);  new base T (2/2);  total (33/19)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGAGGTTGCTTCTGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCC  >  ACB143/2074639‑2074896
                                                                                                                                    |                                                                                                                              
gTGGAGGTTGCTTCTGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCa                                                                                                                            <  2:10556/136‑1 (MQ=255)
         gCTTCTGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAat                                                                                                                    >  5:83678/1‑135 (MQ=255)
         gCTTCTGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAat                                                                                                                    >  1:232580/1‑135 (MQ=255)
          cTTCTGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAatca                                                                                                                  <  4:322630/136‑1 (MQ=255)
           ttCTGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGCGGGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAatca                                                                                                                  >  5:131539/1‑136 (MQ=255)
           ttCTGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAatcat                                                                                                                 >  5:90921/1‑136 (MQ=255)
              tGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTTCGCAAGATAAATCATCGc                                                                                                              >  4:387446/1‑136 (MQ=255)
              tGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTTCGCAAGATAAATCATCGc                                                                                                              >  4:381336/1‑136 (MQ=255)
                cGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCg                                                                                                               <  3:180633/133‑1 (MQ=255)
                   tGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGtt                                                                                                          >  8:289645/1‑135 (MQ=255)
                       cAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATc                                                                                                      <  8:363850/135‑1 (MQ=255)
                             aGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACgg                                                                                               >  1:267724/1‑136 (MQ=255)
                                gACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCa                                                                                            >  1:214044/1‑136 (MQ=255)
                                gACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCa                                                                                            >  4:282903/1‑136 (MQ=255)
                                  ccTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCAtt                                                                                          <  4:192139/136‑1 (MQ=255)
                                  ccTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCAtt                                                                                          >  3:130013/1‑136 (MQ=255)
                                  ccTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCAtt                                                                                          >  4:34766/1‑136 (MQ=255)
                                  ccTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCAtt                                                                                          >  4:112057/1‑136 (MQ=255)
                                  ccTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTGTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCAtt                                                                                          <  2:368839/136‑1 (MQ=255)
                                         cATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAg                                                                                    >  1:174111/1‑135 (MQ=255)
                                                aGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCa                                                                                            >  7:189441/1‑120 (MQ=255)
                                                aGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCa                                                                                            <  8:189441/120‑1 (MQ=255)
                                                        tAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGt                                                                    >  4:29463/1‑136 (MQ=255)
                                                                   gcgcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAgg                                                         >  4:29711/1‑136 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTGGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtt                                                       >  3:361394/1‑136 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATc                                                                                                      >  6:274124/1‑89 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATc                                                                                                      <  5:274124/89‑1 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAAc                                                                                                 >  4:20282/1‑94 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAAc                                                                                                 <  3:20282/94‑1 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCt                                                              >  7:313504/1‑129 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCt                                                              <  8:313504/129‑1 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtt                                                       >  7:116016/1‑136 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtt                                                       >  8:59780/1‑136 (MQ=255)
                                                                     gcTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtt                                                       >  5:273983/1‑136 (MQ=255)
                                                                       tCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtttt                                                     >  4:184671/1‑136 (MQ=255)
                                                                       tCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtttt                                                     >  4:14794/1‑136 (MQ=255)
                                                                       tCCCGTTTCCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGtttt                                                     >  2:243447/1‑136 (MQ=255)
                                                                         ccGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTcg                                                   <  6:273983/136‑1 (MQ=255)
                                                                         ccGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTc                                                    <  6:286117/135‑1 (MQ=255)
                                                                                          gggggTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGt                                   >  5:327645/1‑136 (MQ=255)
                                                                                          gggggTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGt                                   >  1:179076/1‑136 (MQ=255)
                                                                                                          tttAAATTAGCGCGCAATTAGTTAGTTCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTtgctg                   <  3:387446/136‑1 (MQ=255)
                                                                                                          tttAAATTAGCGCGCAATTAGTTAGTTCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTtgctg                   <  3:381336/136‑1 (MQ=255)
                                                                                                          tttAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTtgctg                   <  1:105201/136‑1 (MQ=255)
                                                                                                          tttAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTtgctg                   <  1:268426/136‑1 (MQ=255)
                                                                                                          tttAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTtgctg                   <  2:88877/136‑1 (MQ=255)
                                                                                                          tttAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTtgctg                   <  7:289645/136‑1 (MQ=255)
                                                                                                                  aGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGc            <  2:214044/135‑1 (MQ=255)
                                                                                                                     gcgcAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTc          >  6:313516/1‑134 (MQ=255)
                                                                                                                       gcAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGTTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAgg      >  4:301239/1‑136 (MQ=255)
                                                                                                                       gcAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAgg      >  5:138285/1‑136 (MQ=255)
                                                                                                                           ttagttagTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTAGGCTTGCAGTTGCTGCAACTGCTCAACGATcc  >  8:131064/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                              
GTGGAGGTTGCTTCTGCGATGTACAGCGGAGTGACCTTTTGCATCTTGAGCCTCGCTAATGTGCTGTGCGCTCCCGTTTGCAGGAGGGC‑GGGGGTTGGTGTGGGATTTAAATTAGCGCGCAATTAGTTAGTGCGCAAGATAAATCATCGCCGTTCATCGGAACGGCCATTTCACAGCATAGCGCTCAGAGGTTTTTCTGCAGGTTTTCGCGCAGGTTCAGCAGGTCGGCTTGCAGTTGCTGCAACTGCTCAAGGCTCC  >  ACB143/2074639‑2074896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑