Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,094,817 T→G 38.0% Q183P (CAG→CCG)  CJ019_01995 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,094,8170TG38.0% 34.1 / 20.9 91Q183P (CAG→CCG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/18);  new base G (9/27);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 3.14e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAAGCCCTCATGGGCA  >  ACB143/2094682‑2094951
                                                                                                                                         |                                                                                                                                       
ggggCCATGCGGTCCAGTACGTGGCATTTGGCCCGGGCAAACTTTTACTAAATATACGT‑CAGATGCAATTAATACACCTTCGGCTCGGGGTGCTTGCCAAAATA‑CTCCACGCCGCGCTCGACGGCCAGGCGCGCCt                                                                                                                                         <  4:329673/136‑1 (MQ=255)
    ccATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCCCGCCGGG‑cc                                                                                                                                    >  6:194459/1‑136 (MQ=255)
     cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTTCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑ccc                                                                                                                                   >  3:307623/1‑135 (MQ=255)
     cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCg                                                                                                                                   >  8:105071/1‑136 (MQ=255)
      agggggTCCAGAACCTGGGCATTGGCCGGGGCCAACAGTTTCTCAAGATACGT‑CCGCTGCTCTTCAAACCCCTTCGGCGCCGGCTGCTTGCGCAAATA‑CTCCCCGCCGCGCTCGACGGCCCGGCGCGCCTGG‑CCgg                                                                                                                                  <  6:69377/132‑1 (MQ=255)
        ccGGTCCAGTCCCTGGGCATTGCCCCGGGCCAACAGTTGCTCAACATCCTC‑CTGCTGCCATTCATACACCTTCGGCGCCGGCTGCTTGCGAAAATA‑CTCCCCGCCGCGCTCGATGGCCAGGCGCGCCTGG‑CCGGGc                                                                                                                                <  4:141158/135‑1 (MQ=255)
           gTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTATTCATACACCTTCGGCTCCGGCTCCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCACCCGCCCCAGG‑Ccgggcggg                                                                                                                             >  3:371795/1‑136 (MQ=255)
               aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCTGGccgt                                                                                                                         >  4:266861/1‑134 (MQ=255)
                gTAGCTGGGCATTGGCCGGGCCCATCTTTTTCTAATGTTAGGT‑GCGCGCTTTTTAATACACCTTGGGCGGCGGGGGTTGGCTAAATTA‑CTCCCCCCGGCGCTCGAAGGCCCGCCGCGCCTGG‑CCGGGCTGGCCttt                                                                                                                        <  4:113837/136‑1 (MQ=255)
                 tACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCCGGCTGGCCtttt                                                                                                                       >  5:321475/1‑136 (MQ=255)
                 tACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCCGGCAGGGCtgtt                                                                                                                       >  2:351170/1‑136 (MQ=255)
                 tACCTGGGCATTGCCCCGGCCCAACTGTTTCTAAACATCCGT‑CCGCTGCGCTTCATCCACCTTCGGCTCCGGCTGCTGGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCtttt                                                                                                                       <  6:298531/136‑1 (MQ=255)
                   cgtGGGCATGGGCCGGGCCAAACTGTTTCTAAAGATACGT‑CAGCTGCTCTTCAAACACCTGGGGCGCGGGCTGCTTGCGAAAATC‑CGGCCCGCCGCGCGCGACGGCCCGGCGCGCGGGG‑CCGGGCTGGCCTTTTTg                                                                                                                     <  4:124202/134‑1 (MQ=255)
                    cggggCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCGGCTCTTCATACACCTGCGGCTCCGGCTGCTTGCGCAAATC‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGc                                                                                                                    <  8:295087/134‑1 (MQ=255)
                        gCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGGCCCGGGGGGGCGTTTTTgaggg                                                                                                                 >  5:316203/1‑132 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCAGGCGCCCCGGG‑GCGGGCGGGCCAGTTTGGAGGGc                                                                                                               >  7:78422/1‑136 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGCGGGGCGTTTTTGAgggg                                                                                                               >  3:164545/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGCGGGGCGTTTTGCAgggg                                                                                                               >  6:147618/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCGGGGGGGGCGTTTTTAAGGGc                                                                                                               >  5:141613/1‑136 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGG‑CCGGGCGGGGCGTTTTGCAgggg                                                                                                               >  4:307786/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAgggg                                                                                                               >  2:326633/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCGGGGCGTTTTTCAgggg                                                                                                               >  2:26525/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGG‑GCCGGCGGGGCGGTTTGGAgggg                                                                                                               >  1:313742/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCGGGCGGGCCGTTTTGGAgggg                                                                                                               >  5:90944/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCACGCGCCCCAGG‑GCGGGGGGGCCATTTTGCAGGGc                                                                                                               >  4:64006/1‑136 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑ATGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCGGGCGGGCCTTTTTGACGGGc                                                                                                               >  4:264213/1‑136 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCCGCTGCTTGCGCAAATA‑CTTCACGCCGCGCTCGAAGGCCCCGCCCCCCAGG‑CCGGGCGGGGCGTTTTTGAGGGc                                                                                                               >  3:95841/1‑136 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCATCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCGGGCGGGCCGTTTTGCACGGc                                                                                                               >  6:60394/1‑136 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACAACTTAGGCTACGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGG‑CCGGGGTGGCCGTTTTTCAgggg                                                                                                               >  5:170581/1‑135 (MQ=255)
                         cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCGCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGGGGGCCGGTTTGCCGGGc                                                                                                               >  6:67382/1‑136 (MQ=255)
                                      cAACTGTTCCTAACGATCCGT‑CCGCTGCTCTTAAACCCCCTTCGGCTCGGGCGGCTTGCGAAAATC‑CTGCCCGCCGCGCTCGCCGGCCAGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAgg                                                                                                  <  4:177179/136‑1 (MQ=255)
                                      cAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGGAAATA‑CTGCACCCCGCGCTAGAAGGCCAGGCGCGCCTTG‑CCGGGCTGGCGTTTTTGCAGGGCGTGGTTGGCGAgg                                                                                                  >  6:304237/1‑136 (MQ=255)
                                      cAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCCCGCCTGG‑CCGGGCGGGGCTTTTTTGCGGGACGGTTGGccctgg                                                                                                  >  3:7851/1‑132 (MQ=255)
                                            tttCTCAAGATACGT‑CAGCTGCTCTTCAAACACCTGCGGCTCCGGCTGCTGGCGAAAATA‑CTCCCCGCCGCGCTCGAAGGCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGttgttg                                                                                            <  7:311878/136‑1 (MQ=255)
                                                 cAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCACGCGCGCCGGG‑CCGGGCTGGGCGGTTTGCAGGGCCGGGTGGCCGGGGTTGTTgaagaa                                                                                       >  3:217006/1‑136 (MQ=255)
                                                 acaactACGTCCAGTTGCTCTTAATAAACGTTCGGGGCGGGCTGTTGGCCAAATTA‑CTGCCCGCCGCGGGGGCGGCCCGGGGGCGGCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaaga                                                                                        <  4:248914/131‑1 (MQ=255)
                                                     ctACGT‑CAGCGGCTCGTCATACCCCTTCGGCTCCGGCTGCTTGCGCAAATC‑CTCCCCGCCGCGCTCGACGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAActct                                                                                   <  8:57247/135‑1 (MQ=255)
                                                          t‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCCGGCAGGCCGTTTTTCAGGGCCGGTTGGCCGAGGTTGTTTAATAActcaaaat                                                                               >  2:229769/1‑130 (MQ=255)
                                                          t‑CAGCTGCTCTTCAAACACCTTCGGCTCCGGCTGCTTGCGAAAATA‑CTCCACGCCGCGCTCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                                                              <  1:279848/136‑1 (MQ=255)
                                                          t‑CAGATGCTATACATACACGTTCGGCTCCGGCGGCTTGCGCAAAAA‑ATGAACGCCGCGCTCGACGGCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                                                              <  6:291718/136‑1 (MQ=255)
                                                            cAGCTGCTTATCATACACCTTCGGCTCGGGCCGCTTGCTCTATTA‑CTGCTGGCCGCGGGCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                                             <  7:128050/136‑1 (MQ=255)
                                                            cAGCGGCGCTTAAAACACCTGGGCCGCGGGCGGCTTGCTAAAATC‑CTGCCCCCGGCGCGCGCCGGCCGGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                                             <  6:84401/136‑1 (MQ=255)
                                                                     ttCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCGGGGGGGCCTTTTTTCAGGGCCTGGTGGGCGCGGGTGTTGAAGAACTCGATATGCAGCAGCAcc                                                                    >  1:287948/1‑136 (MQ=255)
                                                                     ttCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCCCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACTCTATATTCAGCAGCAc                                                                     >  1:266410/1‑135 (MQ=255)
                                                                               ttCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCGGGCTGGCCTTTTTGCAGGGCCGGGTGGGCGAGGTTGTTGAAGAAAGATATATGCAGCCGCACCCCGATATGGc                                                          >  8:225063/1‑136 (MQ=255)
                                                                               ttCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGGTGTTGTATAAATCTATATGCAGCAGCACCAGGATATTGc                                                          >  2:22925/1‑136 (MQ=255)
                                                                               tggggCTCGGGGTGCTTGCCAAAATA‑CTCCCCGCCGCGGTCGACGGCCCGGCGCGCGTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                                                          <  4:84954/133‑1 (MQ=255)
                                                                                 gggCTCGGGCTGCTTGCCAAAATC‑CTCCACGCCGCGCTCGACGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCg                                                         <  1:323828/134‑1 (MQ=255)
                                                                                 cgccTCCGGCTGATTGCGCAAATA‑CTGCACCCCGCGCGCGCAGGCCAGTCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                                                          <  6:301670/131‑1 (MQ=255)
                                                                                 cGGCTCGGGGTGCTGGCCAAAATA‑CGCCACGCCGCGCTCGACGGCCGGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                                                          <  8:13377/134‑1 (MQ=255)
                                                                                    cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCTGGGCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTTAAGAACTCTATATTGAGCCTCACCAGGCTATGGCGGAtt                                                     >  4:208711/1‑136 (MQ=255)
                                                                                    cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCGGGTGGCCGAGGGTGTTGTAGAACGCTATATGCAGCAGCACCAGGATATGGCGGAtt                                                     >  5:120065/1‑136 (MQ=255)
                                                                                    cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCGGGGCGTTTTGCAGGGGCGGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                                                     >  3:62615/1‑136 (MQ=255)
                                                                                    cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                                                     >  6:380181/1‑136 (MQ=255)
                                                                                    cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                                                     >  2:61980/1‑136 (MQ=255)
                                                                                    cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCGGTTGGCCGAGGGTGTTTAAGCACTATATATGCACGAGCCCCCGGATATGGCGGAtt                                                     >  2:160399/1‑136 (MQ=255)
                                                                                     ggggggtgcttgctCAAGTA‑CTGCCCGGCGCGGTCGTTGGGCCGGGGGGGGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                                    <  5:347411/130‑1 (MQ=255)
                                                                                     gccGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCCAAGGCCAGGCGCGCGTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                                    <  7:84016/135‑1 (MQ=255)
                                                                                      ccGGGTGATTCCCCAAATC‑CTGCCCGCCGGGCTCTACGGCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTc                                                   <  5:325561/136‑1 (MQ=255)
                                                                                       ggggtgctTGCGAAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                                    <  2:266410/130‑1 (MQ=255)
                                                                                       cggcggGTTGCAAAAATC‑CTCCCCGCGGCGCTCGCCGGCCGGGGGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                                    <  3:264213/134‑1 (MQ=255)
                                                                                       cGGCTGCTTGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                                    <  6:164488/134‑1 (MQ=255)
                                                                                       cGGCTGCTTGC‑TAAATACCGGAACGCCGCGCTCGAACGCCAGGCGCGCCGGG‑CCGTGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGGTGTTCAAGAACTCTATATGAAGCAGCACCAGGATATGGCGGCttt                                                    <  7:238039/134‑1 (MQ=255)
                                                                                          ctggtTGCGCAAATC‑CTGCACGCCGCGGTCGAGGGCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTg                                               <  7:33945/136‑1 (MQ=255)
                                                                                          ctgctTGCCAAAATC‑CTCCCCGCCGCGCGCGACGCCCGGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTg                                               <  3:49971/136‑1 (MQ=255)
                                                                                           tgctTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCGGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGa                                              >  8:248331/1‑136 (MQ=255)
                                                                                            gctTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGAc                                             >  2:125454/1‑136 (MQ=255)
                                                                                                  gcAAATA‑CTGCCCGCCGCGCTCGACGGCCAGGGGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATg                                       <  8:101048/136‑1 (MQ=255)
                                                                                                                gccgccCTCGAAGCCCAGGCGCCCGGGG‑CAGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCCGATTTCCTTGACCCTATGCTTGGCCTCGACa                          <  5:320229/136‑1 (MQ=255)
                                                                                                                gccgcGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGGCTGTTTGCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGAcc                          >  5:259459/1‑135 (MQ=255)
                                                                                                                gccgcGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa                          >  7:227574/1‑136 (MQ=255)
                                                                                                                gccgcGCTCGAAGGCCACGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCACCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa                            >  7:253376/1‑134 (MQ=255)
                                                                                                                gccgcGCGCGAAGCCCCGGCGCCCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa                          <  5:190654/136‑1 (MQ=255)
                                                                                                                cccgcGCTCGACGCCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa                          <  5:184475/135‑1 (MQ=255)
                                                                                                                      cgcgctggCCAGGCGGGCGTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc                    <  2:256447/130‑1 (MQ=255)
                                                                                                                      cgccacggCCCGCCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc                    <  6:141613/130‑1 (MQ=255)
                                                                                                                      cccGACGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc                    <  5:12751/134‑1 (MQ=255)
                                                                                                                      cTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGGCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTGATGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTACTTTACCCAATGCTTGGCCTCGTCAGCggg                     >  6:104833/1‑135 (MQ=255)
                                                                                                                       tCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTACTTTACCCAAAGCTTGGCCTCGACAGCGGGc                    >  4:348505/1‑135 (MQ=255)
                                                                                                                        cgccggCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaa                  <  4:164545/132‑1 (MQ=255)
                                                                                                                         gcgggCCTGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGACTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag                 <  4:155954/133‑1 (MQ=255)
                                                                                                                         gccggCCCGCCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag                 <  1:22925/133‑1 (MQ=255)
                                                                                                                         gatggCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag                 <  8:265845/133‑1 (MQ=255)
                                                                                                                         gacggCCCGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGTGGATTTCCTTGACCCAATGCTTGGCCTCGGCAGCGGGCaag                 <  6:377483/133‑1 (MQ=255)
                                                                                                                         gacggCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag                 <  6:312378/133‑1 (MQ=255)
                                                                                                                         gAAGGCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag                 <  4:309354/136‑1 (MQ=255)
                                                                                                                           tggCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag                 <  2:342547/133‑1 (MQ=255)
                                                                                                                           cggCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag                 <  5:69879/133‑1 (MQ=255)
                                                                                                                              ccgggCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAAGcc            <  3:212415/133‑1 (MQ=255)
                                                                                                                              caagccgcgaCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTTTTGAAGAACTATATATGCAGCATCACCAGGATATGGCGGATTTCCTTGACCCAATTCTTGGCCTCGACAGCGTGCAAGAAGcc            <  5:337985/131‑1 (MQ=255)
                                                                                                                                    cacccTGG‑CCGGGCTGGCCGTTTTTAAGGGACAGTTTGACCAGGTTTTTTAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCATGACCCAATGCTTGGCCTCGACAGCGGGCCAGAAGCCCGCATg      >  6:363109/4‑136 (MQ=255)
                                                                                                                                        cTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTTAAGAACTCTATATGCAGCAGCCCCAGGATATGGCGGATTTACTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAAGACCGCATGGGcc  >  6:48903/1‑135 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAAGCCCTCATGGGCA  >  ACB143/2094682‑2094951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑