Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,348,245 T→G 15.3% E268A (GAG→GCG)  oprJ_1 ← Outer membrane protein OprJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,348,2450TG15.3% 170.1 / 3.0 85E268A (GAG→GCG) oprJ_1Outer membrane protein OprJ
Reads supporting (aligned to +/- strand):  ref base T (5/62);  new base G (11/2);  total (20/65)
Fisher's exact test for biased strand distribution p-value = 2.83e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTCGGTCTCTAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCC  >  ACB143/2348110‑2348379
                                                                                                                                       |                                                                                                                                      
aTGTCGGTCTCTAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCt                                                                                                                                        <  6:392980/136‑1 (MQ=255)
aTGTCGGTCTCTAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCt                                                                                                                                        <  7:139878/136‑1 (MQ=255)
 tGTCGGTCTCTAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTc                                                                                                                                       <  5:329509/136‑1 (MQ=255)
     ggTCTCTAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCCCGGcc                                                                                                                                   >  4:18596/1‑136 (MQ=255)
            aGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCGCCGCCCCCCAgg                                                                                                                            >  7:91713/1‑136 (MQ=255)
             gggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGa                                                                                                                              <  2:201539/133‑1 (MQ=255)
             gggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAg                                                                                                                             <  6:4419/134‑1 (MQ=255)
              ggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCCCCGAGGcc                                                                                                                          >  1:42480/1‑136 (MQ=255)
              ggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCCCCCACGcc                                                                                                                          >  6:104210/1‑136 (MQ=255)
              ggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCGCGGCCCCCGCGGcc                                                                                                                          >  7:27371/1‑136 (MQ=255)
              ggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCGCGGCCCCCCAGGcc                                                                                                                          >  7:380098/1‑136 (MQ=255)
              ggTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCGCCGCCACCCAGGc                                                                                                                           >  2:295411/1‑135 (MQ=255)
                tGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCGCGGGCCCCGCGGCCTg                                                                                                                        >  6:52562/1‑136 (MQ=255)
                tGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTg                                                                                                                        <  1:337990/136‑1 (MQ=255)
                  gccgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTgg                                                                                                                       <  7:92155/135‑1 (MQ=255)
                  gccgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGc                                                                                                                      <  7:236878/136‑1 (MQ=255)
                  gccgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGc                                                                                                                      <  6:276838/136‑1 (MQ=255)
                  gccgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGc                                                                                                                      <  2:334197/136‑1 (MQ=255)
                  gccgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGc                                                                                                                      <  5:198800/136‑1 (MQ=255)
                  gccgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGc                                                                                                                      <  6:276956/136‑1 (MQ=255)
                  gccgccTGCAGTTCCCGTTCGGCCTGGCGCACAGCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGc                                                                                                                      <  4:89209/136‑1 (MQ=255)
                  gccgccTGCAGTTCCCGGTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGc                                                                                                                      <  5:137061/136‑1 (MQ=255)
                  gccgccTGCAGTTCCCGGTCGGCCTGGCGCACATCTGGCCGGCGCTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGGCGTGGCCTCGGCCACCGAGGCCTGGc                                                                                                                      <  2:302901/136‑1 (MQ=255)
                   ccgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCCCGGCCCCCGACGCCTGGcc                                                                                                                     >  3:349018/1‑136 (MQ=255)
                    cgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCCCCGACGCCTGGccc                                                                                                                    >  1:108281/1‑135 (MQ=255)
                    cgccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCa                                                                                                                    <  6:180187/136‑1 (MQ=255)
                     gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCGCGGCCCCCCCGGCCGGGCCa                                                                                                                    >  4:337110/1‑135 (MQ=255)
                     gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCCCCCCGGCCGGGGCa                                                                                                                    >  6:187801/1‑135 (MQ=255)
                     gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCGCGGCCCCCCCGGCCGGGccc                                                                                                                    >  3:283254/1‑134 (MQ=255)
                     gccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCGAGGCCACCGAGGCCTGGGCAc                                                                                                                   >  6:348597/1‑136 (MQ=255)
                      ccTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACt                                                                                                                  <  4:286094/136‑1 (MQ=255)
                      ccTGCAGTTCCCGTTCGGCCAGTCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACt                                                                                                                  <  5:388587/136‑1 (MQ=255)
                       cTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTg                                                                                                                 <  1:234365/136‑1 (MQ=255)
                             ttCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATg                                                                                                           <  5:129571/136‑1 (MQ=255)
                             gtCCTGTTCGGCCTGGCTCACTTCTGGCCGGCGTTCTAGCAAATGCCAGCCATCTGGAGCCGGCAGCGGCTCCGACAGCTTGGGGATGGCTACGCAGGTCGTGGCATCGGCCACGGAGGTCTGGCCTCTGAGcttt                                                                                                           <  4:224045/135‑4 (MQ=255)
                                  gTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCGCGGCCCCCCCGGCCGGGCCACAGAGCATGCTCAg                                                                                                      >  1:43495/1‑136 (MQ=255)
                                        ccTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTa                                                                                                <  4:166822/136‑1 (MQ=255)
                                            gcgcACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAgc                                                                                            <  3:337110/136‑1 (MQ=255)
                                               cacaTCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGcc                                                                                         <  1:117874/136‑1 (MQ=255)
                                                       gCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTgcc                                                                                  <  4:119775/135‑1 (MQ=255)
                                                       gCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTg                                                                                    <  7:73713/133‑1 (MQ=255)
                                                          ggCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCACCGCCCCCCCGGCCGGGCCACAGAGCCTGCTCAGTTAGTAAAGCCCCAAAAgccgccc                                                                              >  7:164541/1‑135 (MQ=255)
                                                            cGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCggg                                                                            <  7:211309/136‑1 (MQ=255)
                                                              ttCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCt                                                                          <  7:339234/136‑1 (MQ=255)
                                                               tCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCTCGGCCCCCCCGGCCGGGCCCCAGAGCATGGGCAGGTCGTTAATCGCCCACAGCCGCCgggggg                                                                         >  7:192187/1‑133 (MQ=255)
                                                               tCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCGCGGCCCCCGCGGGCGGGCCCCTGAGCATGCGCAGGTGGTAAAGCGCCCACAGCCCCCgggggg                                                                         >  4:368678/1‑133 (MQ=255)
                                                                 gAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGGCAAGGCCCCCCCGCCCAGGCCCCTGAGCATGCGGAGTTGGTTAAGCGCCAAATGCCCCCGGGcgggg                                                                       >  5:280196/1‑132 (MQ=255)
                                                                      aaTGCCAGCCATCGCCCGCCGGCAGCGGCACCGACAGCGTGGGGATGTCTACGCAGGGCGTGGCCGCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCAt                                                                  <  7:184233/136‑1 (MQ=255)
                                                                         gccagccaTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCGCGGCCCCCCCGGCCAGGCCACAGAGCCTGCGCAGTTCGGTAATCCCCCACTGCCGCCGGGCTGCGAGCATAgg                                                               >  6:171841/1‑136 (MQ=255)
                                                                           cagccaTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCa                                                             <  5:269955/136‑1 (MQ=255)
                                                                                aTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                        <  3:347418/136‑1 (MQ=255)
                                                                                aTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                        <  1:71555/136‑1 (MQ=255)
                                                                                aTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                        <  3:10528/136‑1 (MQ=255)
                                                                                aTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                        <  7:208907/136‑1 (MQ=255)
                                                                                aTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                        <  7:94941/136‑1 (MQ=255)
                                                                                aTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                        <  5:133229/136‑1 (MQ=255)
                                                                                aTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                        <  1:190561/136‑1 (MQ=255)
                                                                                aTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                        <  3:48540/136‑1 (MQ=255)
                                                                                aTCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTgc                                                        <  5:381141/136‑1 (MQ=255)
                                                                                     cAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGcgcacg                                                   <  6:36760/136‑1 (MQ=255)
                                                                                     cAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGcgcacg                                                   <  5:233447/136‑1 (MQ=255)
                                                                                     cAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGcgcacg                                                   <  3:188267/136‑1 (MQ=255)
                                                                                     cAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCGTGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGcgcacg                                                   <  5:244741/136‑1 (MQ=255)
                                                                                                  cACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGc                                      <  6:48369/136‑1 (MQ=255)
                                                                                                               gggATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgc                         <  5:38881/136‑1 (MQ=255)
                                                                                                               gggATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgc                         <  3:193615/136‑1 (MQ=255)
                                                                                                               gggAGGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGGTCGTACAGCGCCATCTGCCGCCGGGCTGAGAGCCTCGGCAGTTGCGCACGCACCTGTTCCCGCCTGACCAGCAGgc                         <  2:191670/136‑1 (MQ=255)
                                                                                                                 gATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCTCTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCTGTTGCGCACGCTGCTGTTCCCGCTAGGCCAGCAGgcgc                       <  1:78844/136‑1 (MQ=255)
                                                                                                                 gATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgcgc                       <  1:342172/136‑1 (MQ=255)
                                                                                                                 gATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgcgc                       <  4:177812/136‑1 (MQ=255)
                                                                                                                 gATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGgcgc                       <  7:184151/136‑1 (MQ=255)
                                                                                                                      cTACGCAGGTGGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCACGCAAGCCCAGCAGGcgctcgct                  <  5:102817/136‑1 (MQ=255)
                                                                                                                      cTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGcgctcgct                  <  4:80495/136‑1 (MQ=255)
                                                                                                                            aGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAg            <  1:54428/136‑1 (MQ=255)
                                                                                                                            aGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAg            <  6:347762/136‑1 (MQ=255)
                                                                                                                             ggTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg           <  6:316354/136‑1 (MQ=255)
                                                                                                                             ggTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg           <  3:131129/136‑1 (MQ=255)
                                                                                                                             ggTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg           <  1:298852/136‑1 (MQ=255)
                                                                                                                             ggTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg           <  7:303471/136‑1 (MQ=255)
                                                                                                                             ggTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg           <  6:205699/136‑1 (MQ=255)
                                                                                                                             ggTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg           <  3:395663/136‑1 (MQ=255)
                                                                                                                             ggTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg           <  1:150885/136‑1 (MQ=255)
                                                                                                                             ggTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg           <  8:192187/136‑1 (MQ=255)
                                                                                                                             ggTCGTGGCCCCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAgg           <  1:16726/136‑1 (MQ=255)
                                                                                                                                 gTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAg       <  3:384904/136‑1 (MQ=255)
                                                                                                                                    gCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTgg    <  7:63893/136‑1 (MQ=255)
                                                                                                                                      cTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGcc  <  8:205568/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
ATGTCGGTCTCTAGGGTGGCCGCCTGCAGTTCCCGTTCGGCCTGGCGCACATCTGGCCGGCGTTCGAGCAAATGCCAGCCATCGCCAGCCGGCAGCGGCACCGACAGCGTGGGGATGGCTACGCAGGTCGTGGCCTCGGCCACCGAGGCCTGGCCACTGAGCATGCTCAGTTCGTAAAGCGCCATCTGCCGCCGGGCTGAGAGCATCGGCAGTTGCGCACGCACCTGTTCCCGCAAGCCCAGCAGGCGCTCGCTGTCGAGGGCAGTGGCC  >  ACB143/2348110‑2348379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑