Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,365,199 A→C 6.2% intergenic (‑87/‑32) csoR ← / → CJ019_03165 Copper‑sensing transcriptional repressor CsoR/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,365,1990AC 6.2% 668.2 / 3.7 281intergenic (‑87/‑32)csoR/CJ019_03165Copper‑sensing transcriptional repressor CsoR/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (149/106);  new base C (4/22);  total (153/128)
Fisher's exact test for biased strand distribution p-value = 3.78e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

TGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTC  >  ACB143/3365064‑3365333
                                                                                                                                       |                                                                                                                                      
tgatATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGaacaa                                                                                                                                        >  5:62205/1‑136 (MQ=255)
tgatATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGaacaa                                                                                                                                        >  8:134328/1‑136 (MQ=255)
tgatATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGaacaa                                                                                                                                        >  1:389278/1‑136 (MQ=255)
tgatATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCACCTGGGGGGGATATGCTCAAACCAACTACCCCTTCAGaacaa                                                                                                                                        >  3:261782/1‑136 (MQ=255)
tgatATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGGCTCATGCATCGATGAAGTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGaacaa                                                                                                                                        <  8:260737/136‑1 (MQ=255)
     tCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATc                                                                                                                                   >  7:121626/1‑136 (MQ=255)
       gCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATcc                                                                                                                                  <  1:106736/135‑1 (MQ=255)
                ttGGTGCTGATGGCCGTGTTCGTGATCGCTCATTCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCAt                                                                                                                        >  8:330352/1‑136 (MQ=255)
                            gCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAacac                                                                                                            >  3:185819/1‑136 (MQ=255)
                            gCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAacac                                                                                                            >  1:315603/1‑136 (MQ=255)
                                   tCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGc                                                                                                     >  6:226486/1‑136 (MQ=255)
                                   tCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGc                                                                                                     >  6:281582/1‑136 (MQ=255)
                                   tCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGc                                                                                                     >  6:328244/1‑136 (MQ=255)
                                     gTGATCGCTCATGCCTTGAGTCTCATGCATCTATCAATTACGCACGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCACAACAACCATCCGCTTAGGCATCAAAACAAACACGACATGCTc                                                                                                   <  7:28986/136‑1 (MQ=255)
                                        aTCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGc                                                                                                <  7:225179/136‑1 (MQ=255)
                                         tCGCTCATGCGTTGAGTCTCATGCATCTATTAATACCGGTCGCATCATACCCCCCGGGGGGGTATTTGGTCAAACCAACTACCCCTTCACAACACCCATCCGCTTCGGCAACAAAACAACCACGACATGCTCAGc                                                                                                <  6:358847/135‑1 (MQ=255)
                                         tCGCTCATGCCTTGAGTCTCATGCATCTATGAATTACGCTCGCATCCTATCCCCCTGGGGGGTATATGGTCAAACCAACTACCCCTTCACAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGc                                                                                                <  7:283182/135‑1 (MQ=255)
                                         tCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGc                                                                                                <  8:74635/135‑1 (MQ=255)
                                          cGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCaa                                                                                              >  1:357845/1‑136 (MQ=255)
                                             ccATGCCTTGAGTCTCATGCATCTAGCAATTCCGCTCCCATCCAAACCCCCTGGGGGGTATATGGTCAAACCAACTACCCCTTCACAACAACCACCCGCTTTCCCAACAAAACAACCACGACATGCTCAGCAAccc                                                                                           <  8:30486/135‑1 (MQ=255)
                                              cATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAAccc                                                                                           >  4:135756/1‑135 (MQ=255)
                                               aTGCCTTGAGTCTCATGCATCGATTAATTACGCTCGCATCCTATCCCCCTGGGGGGTATATGGTAAAACCAACTACCCCTTCACAACACCCCTCCGCTTCGGCATCAAAACAAACACGACATGCTCAGCAAccc                                                                                           <  7:109989/134‑1 (MQ=255)
                                               aTGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAAcccc                                                                                          >  4:91852/1‑135 (MQ=255)
                                               aTGCCTTGAGTCTCATGCATCGATGAAATACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTAAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCACCAAcccc                                                                                          >  6:124356/1‑135 (MQ=255)
                                               aTGCATTGAGCCTAATCAATCTATTAATTCCGCTCGCATCCTACCCCCGTGGGGGGTATATGGAAAAACCAACACCCCCTGCACAACACCCATCCGCTTCGGCAACAAAACAACCACGACATGCTCAGCAAccc                                                                                           <  2:139908/134‑1 (MQ=255)
                                                          ctcATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAg                                                                              >  5:335711/1‑136 (MQ=255)
                                                          ctcATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAg                                                                              >  5:314880/1‑136 (MQ=255)
                                                              tGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCTCTAAGGTGa                                                                          >  3:92466/1‑136 (MQ=255)
                                                              tGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGa                                                                          >  1:279465/1‑136 (MQ=255)
                                                              tGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGa                                                                          >  7:63080/1‑136 (MQ=255)
                                                              tGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGa                                                                          >  5:101389/1‑136 (MQ=255)
                                                               gCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGAt                                                                         >  4:392747/1‑136 (MQ=255)
                                                                aaTCGATTAATTACGCTCGCATCCTATCCCCCTGGGGGGTATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTTGGAATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGAt                                                                         <  6:144105/134‑1 (MQ=255)
                                                                    gATGAATTCCGCTCCCATCCTACCCCCCTGGGGGGTATATGGCAAAACCAACTCCCCCTTCACAACAACCACCCGCTTCCGAAAAAAACCAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTg                                                                    <  6:10547/136‑1 (MQ=255)
                                                                      tGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGcc                                                                  >  1:203026/1‑136 (MQ=255)
                                                                        aaTTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGccat                                                                >  7:378644/1‑134 (MQ=255)
                                                                        aaTTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCt                                                                >  2:361924/1‑136 (MQ=255)
                                                                              gCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGctct                                                          <  7:55346/136‑1 (MQ=255)
                                                                              gCTCGCATCATACCCCCCTGGGGGGTATATGGTCAAACCACCAACCCCTTCACAACAACCATCCGCTAAGGCATCAAAAGAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGctct                                                          <  1:72146/136‑1 (MQ=255)
                                                                               cTCGCATCCTATCCCCCTGGGGGGTATATGGTCAAACCAACTACCCCTTCACAACAACAATCCGCTTAGGCATCAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTg                                                         <  8:228975/136‑1 (MQ=255)
                                                                                cctcATCCTCTCCCCGTGGGGGTTTTATGTAAAAACAAACACCCCATTCAAAACAACAATCCGTTAAGGCATCAAAATAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTgg                                                        <  2:205809/133‑1 (MQ=255)
                                                                                  gCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGc                                                       >  4:166460/1‑135 (MQ=255)
                                                                                  gCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGc                                                       >  7:85652/1‑135 (MQ=255)
                                                                                  gCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTACAGAACAACCATCCGCTAAGGCATCAAAAGAAACACCACATACTCAGCAACCCCATCAGCACTAAGGTCATTTTTGCCCTAGCTCTCGc                                                       >  1:153634/1‑135 (MQ=255)
                                                                                     tCCTATCCCCCTGGGGGGGATATGTAAAAACAAACTACCCCTTAACAACAACCATCCGCTTAGGCATAAAAATAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATc                                                   <  3:341697/136‑1 (MQ=255)
                                                                                     tCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATc                                                   >  8:198065/1‑136 (MQ=255)
                                                                                      ccTATCCGGCTGGGGGGTATTTGGTCAAACCAACTACCCCTTCTGAACAACGATCCGCTTCGGCCTCAAAAGAACCACGACATGCTCAGCAACCCCATCAGCTCTAAGGTGATTTTTGCCCTTGCTCTGGCCATCt                                                  <  8:186208/136‑1 (MQ=255)
                                                                                          tCCCCGTGGGGGGGATTTGGTCAAACAAACTACCCCTTCAGAACAACCATCCGCTTCGGCATCAAACCAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGAt                                              <  1:251249/136‑1 (MQ=255)
                                                                                          tCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGa                                               >  2:379157/1‑135 (MQ=255)
                                                                                             cccTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGc                                           >  5:150048/1‑136 (MQ=255)
                                                                                             cccTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGc                                           >  8:251030/1‑136 (MQ=255)
                                                                                               gtGGGGGGTATAGGGACAAACCAACTACCCCTACACAACAACCCCCGGCTTCGCCATAAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCt                                         <  3:110014/135‑1 (MQ=255)
                                                                                               cTGGGGGTTATATGGTAAAACCAACAACCCCTTCACAACAACCATCCGCTTAGCCATCAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCt                                         <  3:391426/136‑1 (MQ=255)
                                                                                               cTGGGGGTGATTTGGTCAACCCAACAACCCCTTCAGAACAACCATCCGCTTCGCCCTCAAAACACACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCt                                         <  4:287055/136‑1 (MQ=255)
                                                                                               cTGGGGGGGATATGGACAAACCCACTCCCCAATCACAACAACCATCCGCTTAGGCATCAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCt                                         <  7:353863/136‑1 (MQ=255)
                                                                                                tGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTg                                        >  2:68000/1‑136 (MQ=255)
                                                                                                 gggggggATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCCACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTgg                                       >  5:394249/1‑136 (MQ=255)
                                                                                                 gggggggATATGGTCAAACCAACTACCACTTCAGAACAACCATCCGCTTAGGAATCAAAAGAAACACGACATGATCAGCAAACCCATCAGAACTAAGGTGATTTTTGCACTTGATCTGGCCAGATTGAGAGACTgg                                       >  2:168957/1‑136 (MQ=255)
                                                                                                   gggggATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGCAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGc                                     >  6:85033/1‑136 (MQ=255)
                                                                                                   gggggATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGc                                     >  2:225153/1‑136 (MQ=255)
                                                                                                     gggATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCg                                   >  3:355250/1‑136 (MQ=255)
                                                                                                     gggATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCg                                   >  3:123101/1‑136 (MQ=255)
                                                                                                     gggATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCg                                   >  2:338683/1‑136 (MQ=255)
                                                                                                      gtatatGGACAAACCAACAACCCCTTCACAACACCCATCCGCTTAGCCAACAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCgg                                  <  3:401470/134‑1 (MQ=255)
                                                                                                      ggATATGGTCAAACCAACTCCCCCTTCAGAACAACCATCCGCTTAGGCATCAAACGAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCgg                                  <  8:57710/136‑1 (MQ=255)
                                                                                                      ggATATGGTCAAACCAACAACCCCTTCACAACACCCATCCGCTTAGGCATCAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCgg                                  <  2:112463/136‑1 (MQ=255)
                                                                                                         tatGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGca                               >  4:139925/1‑136 (MQ=255)
                                                                                                          atGGTCAAACCAACTACCCCTTCAGACCAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGCAGATTTTAGCCCTTGCTCTGGCCATCTTGATAGCCTGGCCCGGGcaa                              >  2:298979/1‑135 (MQ=255)
                                                                                                           tGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGcaca                             >  7:129417/1‑136 (MQ=255)
                                                                                                            ggTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCacac                            >  6:200530/1‑136 (MQ=255)
                                                                                                            ggTAAAACCAACTACCCCTTCAGAACAACCATCCGCTTCGGATTAAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGcaca                             <  1:46908/135‑1 (MQ=255)
                                                                                                                aaacaaacTACCCATTCACAACAACCATCCGATTAGGCATAAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        <  5:273800/136‑1 (MQ=255)
                                                                                                                aaaCCAACTACCCCTTCAGAACAACCATCCGCTTAGGAATCAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        <  3:144764/136‑1 (MQ=255)
                                                                                                                aaaCCAACTACCCCTTCAGAACAACCATCCGCTAAGGAATCAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        <  5:128848/136‑1 (MQ=255)
                                                                                                                aaaCCAACTACCCCTTCAGAAAAACCATCCGCTTAGGAATAAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        <  8:154745/136‑1 (MQ=255)
                                                                                                                aaaCCAACTACCCCTTCACAACAACCATCCGCTTAGGCATCAAAAAAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        <  2:41675/136‑1 (MQ=255)
                                                                                                                aaaCCAACTACCCCATCACAACAACCATCCTCTTACTCATCAAAATAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        <  7:145685/136‑1 (MQ=255)
                                                                                                                aaaCCAACACCCCCTACACACAAACCATCCGCTTACCAATAAAAACACACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        <  1:380346/136‑1 (MQ=255)
                                                                                                                aaaCCAACAACCCCTTCAGAACAACCATCCGCTTACCCAACAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        <  3:65906/136‑1 (MQ=255)
                                                                                                                aaaCCAAAAACCCCTACACAACAACCATCCCCTAAGGCAAAAAACCAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        <  3:317300/136‑1 (MQ=255)
                                                                                                                  aCCAACTACCCCTTCAGAACACCCATCCGCTTAGGAATCAAAACAACCACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTgc                      <  7:347016/136‑1 (MQ=255)
                                                                                                                   ccAACTACCCCTTCATAACAACCATCCGCTTCGCCATCAAAACAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTgcg                     <  4:197142/136‑1 (MQ=255)
                                                                                                                   ccAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTgcg                     >  7:237808/1‑136 (MQ=255)
                                                                                                                    cAACTACCCCTTCAGAACAACCATACGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTgcgc                    >  1:126628/1‑136 (MQ=255)
                                                                                                                     aaCTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        >  6:345078/1‑131 (MQ=255)
                                                                                                                     aaCTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGct                   >  4:3298/1‑136 (MQ=255)
                                                                                                                     aaCTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGct                   >  3:80760/1‑136 (MQ=255)
                                                                                                                     aaCTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGct                   >  3:207890/1‑136 (MQ=255)
                                                                                                                       ctcccccTTCCCAACAACCATCCGATTAGCCATCAAAATAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGctct                 <  6:48372/133‑1 (MQ=255)
                                                                                                                          ccccTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACctct                        >  8:211794/1‑126 (MQ=255)
                                                                                                                          ccccTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTa              >  8:92160/1‑136 (MQ=255)
                                                                                                                            ccTTCATAACAACAATCCGATTAGGAATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATg            <  5:184057/136‑1 (MQ=255)
                                                                                                                                 aGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTc       >  5:210925/1‑136 (MQ=255)
                                                                                                                                 aGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTc       >  2:282595/1‑136 (MQ=255)
                                                                                                                                  gAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTc       >  7:189951/1‑135 (MQ=255)
                                                                                                                                  gAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTc       >  8:225641/1‑135 (MQ=255)
                                                                                                                                  gAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCg      >  6:150741/1‑136 (MQ=255)
                                                                                                                                   aacaacCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTc       >  4:223689/1‑134 (MQ=255)
                                                                                                                                     caacCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGt   >  3:299925/1‑136 (MQ=255)
                                                                                                                                     aaacCATCAGCTTCGGCATCAAAACACACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGt   <  1:383773/135‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TGATATCGCTATGACTTTGGTGCTGATGGCCGTGTTCGTGATCGCTCATGCCTTGAGTCTCATGCATCGATGAATTACGCTCGCATCCTATCCCCCTGGGGGGGATATGGTCAAACCAACTACCCCTTCAGAACAACCATCCGCTTAGGCATCAAAAGAAACACGACATGCTCAGCAACCCCATCAGCACTAAGGTGATTTTTGCCCTTGCTCTGGCCATCTTGATAGCCTGGGCCGGGCACACCTCTGCGCTCTTTATGGGTTCGAGTC  >  ACB143/3365064‑3365333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: