| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 4,760,949 | G→C | 15.1% | intergenic (+9/+46) | ligM_KToptimized → / ← lip2 | sequence generated by OPTIMIZER for KT2440/Lipase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,760,949 | 0 | G | C | 15.1% | 403.3 / 14.0 | 199 | intergenic (+9/+46) | ligM_KToptimized/lip2 | sequence generated by OPTIMIZER for KT2440/Lipase 2 |
| Reads supporting (aligned to +/- strand): ref base G (17/145); new base C (8/12); total (42/157) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 204 total aligned reads displayed.
GCAAAACCACCGTAGAGCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGG > ACB143/4760816‑4761083 | gcAAAACCACCGTAGAGCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGc > 4:282920/1‑135 (MQ=255) ccaccGTAGAGCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGAc < 3:312677/136‑1 (MQ=255) ccaccGTAGAGCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGAc < 8:67524/136‑1 (MQ=255) ccaccGTAGAGCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGAc < 7:139151/136‑1 (MQ=255) caccGTAGAGCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGAcc < 7:109151/136‑1 (MQ=255) agagCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCTGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGAGGATGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAACCGCCGGCCGggggg > 1:335793/1‑135 (MQ=255) agagCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTGCAAACCCACCCCCCggcggc > 6:317268/1‑136 (MQ=255) gagCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCt < 3:102097/136‑1 (MQ=255) gagCCGCACAAGCAGATGGCCGTCCGCGCCGTCGCAAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCt < 4:18295/136‑1 (MQ=255) gCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGa < 5:279474/136‑1 (MQ=255) cacgCAGATGGCCGGCCGCGCCGTCGTCAGCCCGGTGCCTTACTCCGTTACTGCCCGTGAAACCTCCGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTc < 2:117248/133‑1 (MQ=255) caAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTc < 8:295550/136‑1 (MQ=255) caAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTc < 6:341822/136‑1 (MQ=255) caAGCAGATGGCCGGCCGCGCCGTCGTCCGCCCGGGGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAACCCTCCCACCGGAGGCTTTTGACTc < 8:343906/136‑1 (MQ=255) aaGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCa < 6:355684/136‑1 (MQ=255) aGCATATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCAt < 7:115776/136‑1 (MQ=255) aGCAGATGGCCGTCCGCGCCGTCGTCTGCCCGGTGCCGTACTCCTTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGATTTTGACTCAt < 5:143458/136‑1 (MQ=255) aGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCAt < 3:225738/136‑1 (MQ=255) aGCAGATGGCCGTCCGCGCCGGCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCAt < 7:121499/136‑1 (MQ=255) aGCAGATGGCCGGCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCAt < 4:354394/136‑1 (MQ=255) gaagagGGACGTCCGCGCCGTCGGCAGCCCGGGGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTAAAAACCCTCCGACCGGCGGCTTTTGACTCATg < 6:218221/134‑1 (MQ=255) gCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATg < 1:61225/136‑1 (MQ=255) gATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAACCCACCCCCCCGAGGCGTTTGACACCTggct > 2:13970/1‑134 (MQ=255) ccGGCCGCGCCGGCGTCAGCCCGTGGCCGTACTCCGTTACTGCCCGTGAAACCTCCGAAGGCGGCGGGCGCAACGCGGCGGTCACCGCCTGAAGTCAAAAGCCGCCGACCGGAGGCTTTTGACTCATGGATGCCTg < 8:70244/136‑1 (MQ=255) gTCCGCGCGGGCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTCAAACCTCCCACGGCGGCTGCCGAAAAGCGGCGGTCACGGCCTCAAGTCAAAACCCCCCGACCGCAGGTTTTTGACTCATGGATGCCTGaa < 6:12211/136‑1 (MQ=255) gTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTTAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGaa < 1:144125/136‑1 (MQ=255) gTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGaa < 1:2552/136‑1 (MQ=255) gTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGaa < 7:2184/136‑1 (MQ=255) gTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGaa < 8:256928/136‑1 (MQ=255) gTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGaa < 2:332882/136‑1 (MQ=255) gTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGGCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGaa < 4:296016/136‑1 (MQ=255) gTCCGCGCCGTCGTCAGCCCGGGGCCGTACTCCGTTAATGCCCGTGAAACCTACGACGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTTAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGaa < 7:31855/136‑1 (MQ=255) gTCCGCGCCGTCGGCAGCCCGGTGCCGTACTCCGTTCCTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGaa < 8:365827/136‑1 (MQ=255) tCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAACCCTCCCCCCGGCGGGGTTTGGCTAATGGATGCCGGaa > 8:282731/1‑135 (MQ=255) ccGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAg < 3:271268/136‑1 (MQ=255) gcgcCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGCCCGGCGGCGTTTGCCTCATGGCTGGCTGAAAGGc > 2:263353/1‑136 (MQ=255) gcgcCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGc < 7:57502/136‑1 (MQ=255) gtcAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTAc < 3:18436/136‑1 (MQ=255) gtcAGCCCGGGGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTAc < 1:315829/136‑1 (MQ=255) cAGCCGGGTGCCGTACTCCGTTACTGCCCGTTAAGCCTACGACGGCGGCGGGCGAAAAGCGGCGGTCACCGCCTGAAGTAAAAACCCCCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAg < 4:19986/136‑1 (MQ=255) aGCCCGGTGCCGAACTCCGTTACTGCCCGGGAAACCTACGAAGGCGGCGGGCGCAAACCGGCGGTCACCGCCTCAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGa < 6:95938/136‑1 (MQ=255) ccgggggCCGTACCCCGTTACTGCCCGTGAAACCTACGACGGCGGGTGGCGCAACGCGGCGGTCACCGCCTCAATTCAAAACCCTCCGCCCGGAGGGTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGAtg < 6:235344/130‑1 (MQ=255) cccGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGAtg < 5:387503/136‑1 (MQ=255) tcccGCACTCCGTTACTGCCCGTGAACCCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCGGAAGTCAAAAGCCTCCGACCGCAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctgct < 6:374096/134‑1 (MQ=255) tGCCGTACTCCGTTACTGCCCGTGAAACCTACGACGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAACCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctgct < 8:143015/136‑1 (MQ=255) tGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctgct < 7:39995/136‑1 (MQ=255) tACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCACCGCCCGGCGGCTTTTGTCTCATGGATTCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGac > 2:337232/1‑136 (MQ=255) tACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGACTACGCCCCGGGGGGTTTTAATCAAGGGTTCCTGAAAGGCTACCATTCAGATGCTGGTGGAGTGCCGCGac > 6:161941/1‑136 (MQ=255) cTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAATCCACCGCCCCGGGGGGTTTGGGGGATGGATGCCGGAAAGGCGGCCGTTAAGATGGTGCTGGAGTGGCTCGccca > 5:155141/1‑136 (MQ=255) cTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGacc < 3:335922/135‑1 (MQ=255) cTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCCGAGGCGTTTTTCTCATGGGTGGCGGAAAGGCGGCCATACAGATTCTGCTGCCGTGGCGCGccca > 3:64797/1‑136 (MQ=255) cTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCGCCGACCGGAGGCGTTTTACTCATGGATGCCTGAAAGGCTCCCGTACAGAATCTGGTGCAGTGGCTCGcccc > 6:63595/1‑135 (MQ=255) cTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCGCCGACCCGCGGCTTTTTACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGacca > 2:272319/1‑136 (MQ=255) cTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAACCACCGCCCGGCGGCTTTTGACTCAAGGATGCCTGAAAGGCTCCCGTACAGATGCTGCTGCCGTGCCTCGccca > 8:61733/1‑136 (MQ=255) cccGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAACCACCCCCCGGGGGGTTTTTTAGAATGGATGCCGGAAAGGCGCCCGTACAGATGCTGCGGCACTGCCACGaccacc > 2:294065/1‑135 (MQ=255) ccGTGAAACCTACGAAGGGGGCTGGCGAAATGCGGCGGTCACCGCCTTAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCAt < 3:359511/136‑1 (MQ=255) gTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAACCACCGCCCCGAGGCTTTTGACTAATGGATGCCGGAAAGGCTGCCTTACAGATTCTGCTGCAGGTCCTCGCCCACCCTCg > 5:320638/1‑136 (MQ=255) gTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAACCACCGACCCGAGGCTTTTGACTCATGGATGCCTGAAAGGCGACCATACAGATGCTTCTGCCCTGCCTCGcccccccacg > 7:372818/1‑132 (MQ=255) gTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAACCAACGCCCCGAGGCGTTTTTCTCATTGATTCCTGAAAGGCTCCCATACAGATTCTGGTGCAGTGCCGCGaccacccaag > 5:353057/1‑132 (MQ=255) gAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCACCCACCGGCGGCGTTTTACTCAATGATGCCGGAAAGGCTCCCCTACAGATTCTGCGGCAGTTCCACGAccaccaaaccc > 5:256669/1‑131 (MQ=255) gAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAACCGCCGCCCCGAGGCGTTTTACTAAATGATTCCGGGAAGGGGCCCTTAAAGATGCTGGTGGAGGGCCGCGCCCACCCACGcc > 4:150653/1‑136 (MQ=255) aaCCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAATCCGCCGCCCGGAGGCTTTTGCCTCATGGATGCCGGAAAGGGGACCGTAAACATGCTGCTGCGGTGCCTCGGCCCCCATCGcccg > 2:322056/1‑134 (MQ=255) aaCCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGGGGCTTTTGACTCATg > 7:270288/1‑79 (MQ=255) aaCCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGGGGCTTTTGACTCATg < 8:270288/79‑1 (MQ=255) aaCCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGCTGCCTGAAAGGCTGCCATACAGATTCTGCAGCAGTGCCGAGCCCACCAACGCCac > 1:309941/1‑135 (MQ=255) aGGCGGCTGGGGCAAAGCTGCGGTCACCGCCTGAAGTCAAATGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATGGCCAGGCCTTCATc < 7:28514/136‑1 (MQ=255) tGGCGCAAAGCGGCGGTCACCGCCTGAAGTAAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGcctcc < 5:154005/136‑1 (MQ=255) gggCGCAAAGCGGCGGTCACCGCCTGAAGGCAAAAGCCTCCGACCGCAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGcctcc < 8:329812/135‑1 (MQ=255) gcgcAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGcctcc < 3:29169/134‑1 (MQ=255) gcgcAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGcctcc < 1:214126/134‑1 (MQ=255) gcgcAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGcctcc < 7:82283/134‑1 (MQ=255) cAAAGCGGCGGGCACCGCCTGAAGGCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCgat < 6:274083/136‑1 (MQ=255) aaaGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCgatg < 1:291305/136‑1 (MQ=255) aaGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCgatga < 6:67197/136‑1 (MQ=255) aaGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCgatga < 4:77242/136‑1 (MQ=255) gcggcgGTCAACGCCTGAAGTCATAAGCCTCCGACTGGAGGCTTTTGACTCATGGATTCCTGAAAGGCTCCCTTTCAGATGCTTCTGCAGTGCCTCGTCCACCATCGCCAGGCCTTCATCGGCCTCCTCTATGATc < 2:305668/136‑1 (MQ=255) cggTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAAc < 5:130198/136‑1 (MQ=255) cggTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAAc < 2:163261/136‑1 (MQ=255) cggTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAAc < 1:231048/136‑1 (MQ=255) cggTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAAc < 3:150465/136‑1 (MQ=255) cggTCACCGACTGAAGTCAAAACCCTCCGACCGCAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAAc < 6:303218/136‑1 (MQ=255) gTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAAccc < 2:381768/136‑1 (MQ=255) cGCCTTAAGTCAAAAGCCTCCGACCGGAGGATTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACg < 7:328441/136‑1 (MQ=255) cGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACg < 2:107360/136‑1 (MQ=255) cGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACg < 2:74123/136‑1 (MQ=255) cGCCTGAAGTCAAAACCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACg < 4:12752/136‑1 (MQ=255) gcatGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGc < 4:336317/133‑1 (MQ=255) gCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATATGc < 3:79282/136‑1 (MQ=255) gCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGc < 1:283928/136‑1 (MQ=255) gCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGc < 7:182489/136‑1 (MQ=255) tGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATg < 3:205965/136‑1 (MQ=255) aGTCAAACCCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAg < 5:55397/136‑1 (MQ=255) aGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAg < 8:162559/136‑1 (MQ=255) atcgCCTCCGACCGGAGGGTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGTTGCTGCTGCTTTTCCTCGAAGACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGATCCCATAGGCTTGAAGTCATg < 3:234409/133‑1 (MQ=255) aacgCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATg < 4:166945/133‑1 (MQ=255) aaaGCCTCCGACCGTAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACTCATGAAGTCATg < 6:387085/136‑1 (MQ=255) aaaGCCTCCGACCGGTGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATg < 3:385507/136‑1 (MQ=255) aaaGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATg < 1:194301/136‑1 (MQ=255) aGCCTCCGACCTGATGATTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATggg < 3:394258/136‑1 (MQ=255) aGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGACAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATggg < 3:400344/136‑1 (MQ=255) | GCAAAACCACCGTAGAGCCGCACAAGCAGATGGCCGTCCGCGCCGTCGTCAGCCCGGTGCCGTACTCCGTTACTGCCCGTGAAACCTACGAAGGCGGCTGGCGCAAAGCGGCGGTCACCGCCTGAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGG > ACB143/4760816‑4761083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |