BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	4,765,167	T→G	7.9%	T2414P (ACC→CCC)	grsB ←	Gramicidin S synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,765,167	0	T	G	7.9%	685.3 / 6.1	254	T2414P (ACC→CCC)	grsB	Gramicidin S synthase 2
Reads supporting (aligned to +/- strand): ref base T (104/123); new base G (25/2); total (129/125)
Fisher's exact test for biased strand distribution p-value = 1.99e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

TGTTGATGAACAGGCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTC  >  ACB143/4765032‑4765302

tgtTGATGAACAGGCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGgt                                                                                                                                         <  7:218561/136‑1 (MQ=255)

        aaccgcccccGCATGCTCTCCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGATGAggg                                                                                                                                 <  3:248935/130‑1 (MQ=255)

        aaCAGGCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAggg                                                                                                                                 <  7:141953/136‑1 (MQ=255)

        aaCAGGCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAggg                                                                                                                                 <  1:77472/136‑1 (MQ=255)

        aaCAGGCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAgg                                                                                                                                  <  7:174234/135‑1 (MQ=255)

        aaCAGGCCCAGCAGGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAgg                                                                                                                                  <  6:251615/135‑1 (MQ=255)

         aCAGGCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAggg                                                                                                                                 <  2:271307/135‑1 (MQ=255)

         aCAGGCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAggg                                                                                                                                 <  3:288566/135‑1 (MQ=255)

          cAGGCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTg                                                                                                                               <  2:119053/136‑1 (MQ=255)

            ggCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGATGTTGAGGGTGAt                                                                                                                             <  5:374295/136‑1 (MQ=255)

             gCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATg                                                                                                                            <  8:20913/136‑1 (MQ=255)

             gCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCCCCCCCCGGGCGCCAAAGCAGGCACAACCCAGGGCACGGTAGCGGGCGCGCACCCACAACCACCCCCCCCACACCCCCGTGTTTAgggggtat                                                                                                                            >  4:223866/1‑131 (MQ=255)

             gCCCAGCATGCTCTGCACACCCGGCAAGGCGGCAGGGCGCCCCCCCACCGTGGCGCAAAACCTGAAACTGCGCAGGCCGCTGTAGCGTCGTAGCCCCAACACACAGGCGCCCTGCCCCACGCTGTTGAGGGTGagt                                                                                                                            >  6:56777/1‑134 (MQ=255)

               ccAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATgcg                                                                                                                          >  3:339607/1‑136 (MQ=255)

               ccAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGGGTTGAGGGTGATgcg                                                                                                                          >  7:123582/1‑136 (MQ=255)

               ccAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCAGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCAGCCCCACCGTGTTGAGGGTGGTgcc                                                                                                                          >  8:176954/1‑135 (MQ=255)

                  gCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTg                                                                                                                       <  5:275852/136‑1 (MQ=255)

                  gCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTg                                                                                                                       <  4:126251/136‑1 (MQ=255)

                  gCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTg                                                                                                                       <  5:146592/136‑1 (MQ=255)

                    aTGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCCCCCCCGTGGCGCCAAAGCTGGCACTGCGCTGGCCGCGGTAGCGGCGGGGCCGCCACAACCCGGCGCCCGGCCCCACGGGGTTGTGGGGGATGCGCgggg                                                                                                                     >  6:291083/1‑136 (MQ=255)

                      gCTCTGCACACCCGGCAAGGCGGCCGGGGGCCCCGCCCCCCTGGCGCCAAAGCAGACACTGCGCTGGCCCCTGTATCGGGTGACCCGCACCAACCACGGGCCCGGGACCACGGGGTTGAGGGGGCTGCGGGGGGag                                                                                                                   >  4:326770/1‑135 (MQ=255)

                          tGCACACCCGGCAAGGCGGCCGGGGGCCCCCCCCCCCTGGCGCCCAAGCGGCCACAGCGCTGGCCCGAGTTGCGGCGGAGCAGCAAAAACCACGCCCCCGGCACCCCGGTGTTGTGGGTGATGGGGGGGGATtgtg                                                                                                               >  8:146888/1‑136 (MQ=255)

                          tGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCGGGGATtgtg                                                                                                               >  7:358583/1‑136 (MQ=255)

                          tGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCCTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGGGGTGGGGGGGAGGCGCGGGGGTtgtg                                                                                                               >  5:8216/1‑136 (MQ=255)

                            cacaCCCGGCAAGGCGGCCGGGCGCCCCGCCCCCGTGGCGCCAAAGCTGACACTGCGCAGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGGGTTGAGGGTGATGCGGTGGGATTGTGc                                                                                                              >  7:318455/1‑135 (MQ=255)

                            cacaCCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATtgtg                                                                                                               <  8:304961/134‑1 (MQ=255)

                            cacaCCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATtgtg                                                                                                               <  2:90108/134‑1 (MQ=255)

                            cacaCCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGc                                                                                                              <  3:15116/135‑1 (MQ=255)

                               acCCGGCAAGGCGGCCGGGCGCCCCGCCCCCCTGGCGCCAAACCTGACACTGCGCTGGCCGCTGTAGCGGCGGCGGAGCCACAAACACGCGCCCAGCCCCACCGTGGTGAGGGGGGTGCGCGGGGGTTGTGCGGAg                                                                                                          >  6:264876/1‑136 (MQ=255)

                               acCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGGGCCAAAGCTGCCACTGCGCTGGCCGCTGTAGCGGCTGAGCCGCAACAACCAGGCGCCCGGCACCACCGTGGTGAGGGTGAGGCGCTGGGATTGTGCGTAg                                                                                                          >  8:193557/1‑136 (MQ=255)

                                   ggCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTTGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCt                                                                                                      <  4:98485/136‑1 (MQ=255)

                                   ggCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCt                                                                                                      <  2:209055/136‑1 (MQ=255)

                                   ggCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCt                                                                                                      <  4:378280/136‑1 (MQ=255)

                                   ggCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCt                                                                                                      <  1:387650/136‑1 (MQ=255)

                                   ggCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCt                                                                                                      <  1:133783/136‑1 (MQ=255)

                                   ggCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCt                                                                                                      <  1:241272/136‑1 (MQ=255)

                                   ggCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCt                                                                                                      <  5:156275/136‑1 (MQ=255)

                                    gCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGCCACTGCGCTGGCCGCGGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCtt                                                                                                     >  7:33635/1‑136 (MQ=255)

                                    gCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCCGCAACAACCCCGCGCCCTGCACCACGGGGTTGAGGGGGATGCGCTGGGATTGTGCGAAGGCCtt                                                                                                     >  2:329359/1‑136 (MQ=255)

                                    gCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACCACCAGGCGCCCTGCCCCACGGGGTTGAGGGTGATGCGCGGGGATTGTGCGAAgggcgt                                                                                                     >  2:76611/1‑132 (MQ=255)

                                    gCAAGGCGGCCGGGCGCCCCCCCCCCGTGGCGCCAAAGCGGACACAGCGCTGGCCGCGGGAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCCCCACGGGGGTGAGGGTGCTGCGCGGGGGTTGTGGGAAGGGCtt                                                                                                     >  2:310186/1‑136 (MQ=255)

                                     cAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCGGAGCAGCAACAACCAGGCGCCCTGCACCCCGGTGTTGAGGGTGCTGCGGGGGGATTGTGCGAAGGCCtg                                                                                                     >  8:186930/1‑134 (MQ=255)

                                       aGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGaa                                                                                                  >  6:77186/1‑136 (MQ=255)

                                       aGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCGGACACAGCGCTGGCCCCTGTAGCGGCTGCGCAGCAACAACCACGCGCCCTGCACCACGGGGTTGTGGGGGATGCGCGGGGATTGTGCGAAGGCCATGa                                                                                                   >  5:170336/1‑135 (MQ=255)

                                        ggcggcCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAAc                                                                                                 <  8:79180/136‑1 (MQ=255)

                                              cGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCg                                                                                           >  5:182158/1‑136 (MQ=255)

                                                ggCGCCCCGCCCCCCTGGCGCCCAAGCTGACACAGCGCTGGCCGCGGTAGCGGCTGAGCAGCAACAACCACGCGCCCGGCCCCACCGGGGTGAGGGTGCTGGGCGGGGTTTGTGCGAAGGCCATGAACGTCTCggg                                                                                         >  2:371928/1‑135 (MQ=255)

                                                  cgcCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCt                                                                                       <  2:294656/136‑1 (MQ=255)

                                                  cgcCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCt                                                                                       <  5:203212/136‑1 (MQ=255)

                                                  cgcCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCGGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCt                                                                                       <  4:38167/136‑1 (MQ=255)

                                                          cACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCGGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGaca                                                                               >  8:3795/1‑136 (MQ=255)

                                                             cttGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGTATTGTGCGATGGCCTTGAACGTCTCGGTATGCGCGACACTg                                                                            <  7:75049/134‑1 (MQ=255)

                                                             cGTGTCGCCAAAGCTGACACTGCGCTGGCCGCTGTTGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTg                                                                            <  6:183624/136‑1 (MQ=255)

                                                             cGTGGGGCCAAAGCTGACACTGCGCTGGCCGCTGTTGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCAGGGTTTTGAGGGTGATGCGCTGGGTTTGTGCGAAGGCGTTGAACGTCTCGGGCTGCGCGACACTg                                                                            <  7:157979/136‑1 (MQ=255)

                                                             cGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTg                                                                            <  2:102882/136‑1 (MQ=255)

                                                             cGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTg                                                                            <  4:184878/136‑1 (MQ=255)

                                                             cGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTg                                                                            <  8:129232/136‑1 (MQ=255)

                                                             cGTGGCGCCAAAGCTGACACTGCGCTGGCCGCGGTAGCGGCTGAGCAGCAAAAACCAGGCGCCCTGCACCACGGTGTTGTGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTg                                                                            <  2:189995/136‑1 (MQ=255)

                                                                ggCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGaaa                                                                         <  3:282834/136‑1 (MQ=255)

                                                                 gcgcCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAAc                                                                        <  7:298946/136‑1 (MQ=255)

                                                                  cgcCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACACCCAGGCGCCCTGCCCCACGGTGTTGAGGGTGATGCGCTGGGAGTGTGCGAAGGCCTTGCACGTCTCGGTCTGCGCGCCACTGAAGCg                                                                       >  7:268630/1‑136 (MQ=255)

                                                                  cgcCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACg                                                                       >  6:340818/1‑136 (MQ=255)

                                                                  cgcCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACg                                                                       >  1:223484/1‑136 (MQ=255)

                                                                  cgcCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTGGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACg                                                                       >  6:158349/1‑136 (MQ=255)

                                                                   gcCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGCGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACgg                                                                      >  7:242235/1‑136 (MQ=255)

                                                                     cAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCt                                                                    <  3:191335/136‑1 (MQ=255)

                                                                      aacgCTGACACGGCGCTGGCCGCTGTCGCGGCGTAGCAGCAACAACCCGGCGCCCTGCACCCCGGTGTTGAGGGTGATGCGCGGGGATTGGGCGAAGGCCTTGAACGTCTCGGTCGGCGCGACACTGAAACGGCTg                                                                   <  3:5796/133‑1 (MQ=255)

                                                                      aaaGCTGACACTGCGCTGGCCGCGGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTg                                                                   <  2:147262/136‑1 (MQ=255)

                                                                       aaGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAAc                                                                                                 <  6:359384/105‑1 (MQ=255)

                                                                                cTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCt                                                         >  2:289517/1‑136 (MQ=255)

                                                                                      tGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTg                                                                                                                       <  5:357868/68‑1 (MQ=255)

                                                                                      tGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGc                                                   >  5:53473/1‑136 (MQ=255)

                                                                                      tGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGc                                                   >  2:154119/1‑136 (MQ=255)

                                                                                      tGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGc                                                   >  6:302215/1‑136 (MQ=255)

                                                                                      tGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGc                                                   >  4:309897/1‑136 (MQ=255)

                                                                                      tGGCCGCTGTAGCGGCTGAGCAGCAACAACAAGGCGCCCAGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTCCGCGACACTGACACGGATGTAGAGGGCCTGCTGGc                                                   >  1:165495/1‑136 (MQ=255)

                                                                                           gCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTg                                              >  2:251067/1‑136 (MQ=255)

                                                                                           gCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTg                                              >  7:46294/1‑136 (MQ=255)

                                                                                               tAGCGGCGTAGCAGCAAAAACCCGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGGCGCGGTCTGCGCCACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCcgc                                          <  2:341486/136‑1 (MQ=255)

                                                                                                    gCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGAGGCGCTGGGTTTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCa                                     <  1:341253/136‑1 (MQ=255)

                                                                                                       gAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCg                                  >  5:339178/1‑136 (MQ=255)

                                                                                                       gAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCg                                  >  6:21619/1‑136 (MQ=255)

                                                                                                          catcaacaaCCAGGCGCCCTGCACCAAGGTTTTGTGGGTGATGCGCGGGGATTGTGCTAAGGCTTTGAACGTCTCGGTCTGCGCGACACTTAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCtt                               <  5:304764/133‑2 (MQ=255)

                                                                                                          cagcaACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTgg                                                                                                                      <  4:59317/49‑1 (MQ=255)

                                                                                                          cagcaACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTg                               >  2:22488/1‑136 (MQ=255)

                                                                                                          cagcaACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTg                               <  3:320286/136‑1 (MQ=255)

                                                                                                          cagcaACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTg                               >  3:95122/1‑136 (MQ=255)

                                                                                                          cagcaACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTg                               >  3:165877/1‑136 (MQ=255)

                                                                                                                  acCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAgg                        >  6:132018/1‑135 (MQ=255)

                                                                                                                  acCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAgg                        >  1:255733/1‑135 (MQ=255)

                                                                                                                    cAGGCGCCCTGCAACACGGTGTTGAGGGGGTGGCGCTGGGTGTGTGCGAAGGCCTTTCACGTCGCGGTCGGCGCGACACTGACACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCAt                     <  8:287793/136‑1 (MQ=255)

                                                                                                                          cccTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGc                 >  3:366/1‑134 (MQ=255)

                                                                                                                          cccTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGc                 >  7:61298/1‑134 (MQ=255)

                                                                                                                               aaccacGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCaggt            <  3:307720/133‑1 (MQ=255)

                                                                                                                                accacGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCaggtagg         >  7:131434/1‑136 (MQ=255)

                                                                                                                                 ccacGGTGTTGCGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCaggtaggt        <  7:199017/136‑1 (MQ=255)

                                                                                                                                      gtgtTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCt   <  1:307218/136‑1 (MQ=255)

                                                                                                                                       tgtTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTg                                                                            >  4:282696/1‑62 (MQ=255)

                                                                                                                                       tgtTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTc  >  4:145542/1‑136 (MQ=255)

                                                                                                                                       tgtTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGCCATCGGCCAGGTAGGTCGGCTc  >  5:254853/1‑136 (MQ=255)

TGTTGATGAACAGGCCCAGCATGCTCTGCACACCCGGCAAGGCGGCCGGGCGCCCCGCCACCGTGGCGCCAAAGCTGACACTGCGCTGGCCGCTGTAGCGGCTGAGCAGCAACAACCAGGCGCCCTGCACCACGGTGTTGAGGGTGATGCGCTGGGATTGTGCGAAGGCCTTGAACGTCTCGGTCTGCGCGACACTGAAACGGCTGTAGAGGGCCTGGTGGCCGCTGCCGCGCTCACCGCTGGCGAAGGCATCGGCCAGGTAGGTCGGCTC  >  ACB143/4765032‑4765302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`