Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB143 | 4,772,546 | T→G | 45.0% | intergenic (‑140/+21) | grsB ← / ← lgrB_1 | Gramicidin S synthase 2/Linear gramicidin synthase subunit B |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 4,772,546 | 0 | T | G | 45.0% | ‑3.5 / 34.7 | 86 | intergenic (‑140/+21) | grsB/lgrB_1 | Gramicidin S synthase 2/Linear gramicidin synthase subunit B |
Reads supporting (aligned to +/- strand): ref base T (3/42); new base G (31/3); total (36/50) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.37e-15 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01 |
CATCTCCTACCAGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACAC > ACB143/4772412‑4772668 | cATCTCCTACCAGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGt > 2:97454/1‑135 (MQ=255) cATCTCCTACCAGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGt > 3:242729/1‑135 (MQ=255) cTACCAGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGGCCGGGCATagcc > 5:125432/1‑133 (MQ=255) cTACCAGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGGCCCGGCGTACCg > 4:341766/1‑136 (MQ=255) cTACCAGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCCGGCATaacc > 7:299817/1‑133 (MQ=255) cTACCAGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCCGGCATAACg > 4:331883/1‑136 (MQ=255) cTACCAGGGCACCGGTATTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGTGCCGGGCATaacc > 7:276163/1‑133 (MQ=255) aCCAGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGCAAACCcgg > 1:135259/1‑136 (MQ=255) gggccACCGGTCTTTGCCTGCGG‑AACGTGCCCGCCCGCGACAGGCTCATCTTGCTGCCTAAAAAATGAACGCGCCGGACGGCTGCCGGCTCTGGGTGTACTGACGTTGACGACCC‑GGGCCCGGCCATACCGGGccc < 8:315622/132‑1 (MQ=255) cgggCACCGGGCTTTGCCTGCGG‑ACGGTCCCAGCCCGCGACAGGCTCTGGATCCTTCCCAAAAAATCAACGAGCCGGACGGCGGCCGGCTCTGGGTGTACTCAAGTTGACGAGCC‑GGGCCCGGTAATACCGGGccc < 2:305632/135‑1 (MQ=255) aGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGGCCGGGCCTACCGGGccc > 5:157364/1‑136 (MQ=255) aGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGccc < 3:331883/136‑1 (MQ=255) gccccGGTCTTTCCCTGCGG‑ACCGTGCCAGCCCGCGACAGGCTCATGTTGCTGCCGAACAAATGAAAGACCCGGACGGCTGCCGGCTGTGGGTGTACTCAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCa < 6:177281/133‑1 (MQ=255) gccccGGTCTTTCCCTCCGC‑AACGTCCCAGCCCGCGACAGGCTCATGATCCTCCCCAACAAATCAAAGCCCCGCACGGCGGCCGGCTCTGGGTGTACTCAAGTTGACGAGCC‑GGGCCCGGTAATACCGGGCCCGCa < 6:129206/133‑1 (MQ=255) gCACCGGGTTTTGCCTGCGA‑ACCGTGCCCGCCCGCAACAGGCTCATGATCCTCCCGAACAAAGGAACCAGCGGGCCGGCTGCCGTCTCTGTGTGTAGTGAAGTTGAGGAGCC‑GGGCCCGGTCATACCGGGCCCGCa < 8:177616/136‑1 (MQ=255) gCACCGGGCTTTGCCGGCGG‑AACGGGCCAGCCCGCGACAGGCGCATGAGGCTGCCGACCAACTGACCGCGCCGGACGGCTGCCGGCTCTGGGGGTCCGGACGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCa < 6:56544/136‑1 (MQ=255) aacGGTCTTTGCCTGCGG‑AAAGTTCCAGCCCGCGACAGGATCATGATGCTGCCGAACAAATGAAAGAGACGGACGGCTTCCGTCTATGGGTGTAATGAAGTTGACGAGCC‑GGGGCCGGTGAGAACGGGacccg > 7:287746/3‑131 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGGCTCACCGGGccccccg > 6:20421/1‑132 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGGCGGACCGGcccccc > 2:17901/1‑132 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGCAATACC‑GGCCCGCcga > 3:156912/1‑136 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGGCCGGGGATACCCGGccccccca > 5:160281/1‑131 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGGCCCGGGATAACCGGccccccgc > 7:42225/1‑131 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGGCCCGGCATACCGGGCCCGccgc > 5:364272/1‑133 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGGCTACCGGGccccccga > 2:243425/1‑131 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGGCTACCCGGCCCGccgc > 2:278982/1‑133 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGGATACCGGGCCCGCagc > 7:101339/1‑135 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGCCATCCGGGcccccc > 7:110406/1‑131 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGCATAACCGGCCCGCa > 7:68434/1‑134 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCCGGGCTACCGGGCCCGccgc > 6:156738/1‑133 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCCGGGATACCCGGcccccccc > 1:96402/1‑131 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCCGGCCAACCCGGcccccccc > 8:217237/1‑131 (MQ=255) aCCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCAGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGCATAGCGGGCCCCCagc > 2:302615/1‑135 (MQ=255) gggcTTTGCCTGCGC‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGCGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAgcg < 7:243693/133‑1 (MQ=255) ggTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGGATACCGGGCCCCCCGAgcg > 2:15768/1‑136 (MQ=255) ggTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGGAGAACGGGGCCGCcgcgcg > 1:13751/1‑136 (MQ=255) ggTCTTTGCCGGCGC‑ACCGGGCCAGCCCGCGACAGGCTCATGATGCTGCCTAACAAATCAAAGCGCCGGCCGGCTGCGGGCTGTGGGTGTACTCACGTTCACGAGCC‑GGGCCCGGTCAAACCGGGCCCGCAGAgcg < 8:108524/136‑1 (MQ=255) ggTCTTTGCC‑GCGGCACCGGCCCCGCCCGCGACAGGCTCATGATGCTGCCGAACAAATCAAAGCGCCGCCCGGCGGCCGGCTGTGGGTGTACTGAAGTTGACGAGCC‑GGGCCGGGTAATCCCGGGCCCGCAGAgcg < 8:76752/136‑1 (MQ=255) ggTCTTGCCCTGCGC‑AACGTCCCCCCCCGCGACAGGCCCAGGAGCCGGCCAAACAAATGAACCCCCCGCACGGCTGCCGGCTCTGGGGTTACTGACGTTGCCGCGCC‑GGGCCCGGTAATACCGGGCCCGCAGAgcg < 6:269121/136‑1 (MQ=255) gTCTTTCCCGGCGG‑ACCGTGCCAGCCGGCGACAGGCTCTTCATGCTGCCCAACAAATGACAGCGCCGGCCGGCTGCCGGCTCTGGGGGTCCTTAAGTAGACGCGCC‑GGGCCCGGTCAAACCGGGCCCGCAGAgcgc < 7:67804/136‑1 (MQ=255) cattGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCCGCCAGAGCGGGCGCCCAGAccgc > 3:94565/3‑134 (MQ=255) tttGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCCGGCAAGCCCGGCCCGCcgcgcgggt > 6:273465/1‑132 (MQ=255) cTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGGCCCGGGATAACCGGCCCCCCCAGCGCGACggggg > 1:11546/1‑135 (MQ=255) gtaccGTGCCCGCCGGCGACTGGCTCTTGATGCTCCCAAACAAAGGAACGCGCGGGCGGGCGGCGGGCTCTGGGTGTACTGACGTTGACGACCG‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAAc < 6:186366/132‑1 (MQ=255) aaCGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGGGCGGGGGTACAGGGCCCCCCGCGCGCGGCGGGGTCGAc > 7:349190/1‑134 (MQ=255) aaCGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATTACAGGCCCGCAGAGCCCTAATGGGGGAACtc > 8:128890/1‑136 (MQ=255) aCGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcg < 7:226236/136‑1 (MQ=255) gggCCAGCCCGCTACAGGTTCATTATGCTGCCTAAAAAATGAAAGAGCGGGACGGGTGCCGGCTCTGGGTTTACTGAAGTTCACGAGCC‑GGGCCGGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc < 3:344996/134‑1 (MQ=255) gTGCCCCCCCGCGACAGGCTCATGAGGATGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGCCGCGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc < 6:224576/136‑1 (MQ=255) gTGCCACCCCGCCACCGGCTCTTTATGCTCCCCAACAAATCAAAGCGCCGGCGGGCTGCCGGCTGGGGGTGTCCTCACGTTCACGCCCC‑GGGCCCGGGCATCCCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc < 8:160900/136‑1 (MQ=255) gcccggCCCGCGACAGGCTCATGATGCGGCCGAACAAATGAAAGAGCCGGACGGCGGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc < 7:294183/130‑1 (MQ=255) cccAGCCCGCGACAGGCTCTTGATGCTGCGGAACAAATGAAAGACCCGGACGGCTGCCGGCTCTGGGTGTACTGACGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc < 8:79791/133‑1 (MQ=255) cAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGGATACCGGGCCCCCCGCGCGCGACTGGGGGAACTCGTCGAtc > 1:243019/1‑136 (MQ=255) cgCCGGCGACAGGCTCTTGATGCTCCCTAAAAATTCAAAGCGCCGCAGGGGTGCGGGCTGTGGGTTTCCTTATGTGGACTCGCC‑GGGCCCGGAAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGAtct < 2:16589/135‑1 (MQ=255) cgCCCGCGACAGGCTCATGATGCTGCCGAACAAATCAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGAtct < 1:69658/135‑1 (MQ=255) ggCTCATGTTGCGGCCTAAAAATTCAACGCGCGGGAGGGGTGCCGGGTGTGGGTTTACTGACGATGCCGCCCC‑GGGCCCGTTCATACGGGCCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGt < 4:163258/135‑1 (MQ=255) ctaaCAAATGAACGCGCCGGACGGCTGCGGGCTCTGGGTGTACTTAAGTTGACGCCCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcg < 6:366786/134‑1 (MQ=255) cGAACAAATTAACGCCCCGGACGGCGGCGGGGTCTGGGTGTACTGAAGTTGACCAGCC‑GGGCCGGGTCATCCCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcg < 7:108803/136‑1 (MQ=255) cGAACAAATGAACGAGCCGGCCGGGGGCGGGCTCTGGGGGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcg < 8:30858/136‑1 (MQ=255) cGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGGATACCGGGCCCCCCGAGCGCGACTTGGGGAACTCGGAGATGTCCGCTGCAGTTAGAAGGTGCAAggcg > 3:156689/1‑136 (MQ=255) gAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTGAACTAGGAGATATACTCTGCAGTTATACGTTTAAAtgcgg > 6:115106/1‑136 (MQ=255) gAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGGCATACCGGGCCCGCCGACCGCGACGGGGTGAACTACTGGCTCTACGCTGGAGGAAGACGTTTGAAggcgg > 4:151098/1‑136 (MQ=255) gAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCCGGCAGAACGGGCCCGCCGAGCGCGACAGGGGGACCTCGTCGAGCGCCACTGGACTAAGACGGTTGAAggcgg > 2:126833/1‑136 (MQ=255) aaaattGAACGCGCCGGAGGGCTGCGGGCTCTGGGGTTACTCACGTGCCCGAGCG‑GGGCCCGGTAATACGGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcgg < 4:130920/129‑1 (MQ=255) aCAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCCGGGCTACCCGGCCCCCACCCCCCGACTGGGGGCACGCGGCGCTCGCCGCGGCCGTAAGCCGGTTAAAGGCGGcc > 7:125397/1‑136 (MQ=255) gCCGGAGGGGGGCCGGCTCTGGGTTCACTTAAGTTCCCGCGCG‑GGGCCCGGTCATCCCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGcc < 2:135214/136‑1 (MQ=255) cTGCCGGCTCTGGGTGTACTGAAGTGGACGCGCC‑GGGCCCGGTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTcc < 1:63899/136‑1 (MQ=255) ggCTCTGGGTGTACTGAAGTTGACGCGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAAt < 1:3778/136‑1 (MQ=255) ggCTCTGGGTGTACTGAAGTTGACGCGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAAt < 4:93543/136‑1 (MQ=255) ggCGCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAAt < 7:123907/136‑1 (MQ=255) gcgcggggTGAACTTAAGTGGACGACCC‑GGGCCCGGAAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATc < 4:166478/131‑1 (MQ=255) gCTCTGGGTGTACTGAAGTTTACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGAAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATc < 6:289641/136‑1 (MQ=255) ggggGTACTGAAGTTGCGGCGCC‑GGGCCGGGCAATCCGGGCCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc < 8:158194/136‑1 (MQ=255) gggTGTACGCAAGTTGACGCCCC‑GGGCCCGGTAATACCGGCCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc < 6:357578/136‑1 (MQ=255) ggtcaaCTGAAGTTGACGACCC‑GGCCCCGTTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc < 7:288584/130‑1 (MQ=255) ggTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc < 5:156738/135‑1 (MQ=255) ttgtACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc < 8:207441/135‑1 (MQ=255) gttcaCTGAAGTTGCCGCGCC‑GGGCCCGGCCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc < 8:36110/132‑1 (MQ=255) gtgtAGTGAAGTTGACGAGCC‑GGGCCCGGTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc < 7:353134/136‑1 (MQ=255) gtgtACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc < 7:300780/136‑1 (MQ=255) gtgtACTCAAGTTGCCGAGCC‑GGGCCCGGGCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc < 8:212245/136‑1 (MQ=255) ttACTGAAGTGGCCGAGCC‑GGGCCGGGTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccc < 1:128251/135‑1 (MQ=255) gcagtGAAGTTGACGCGCC‑GGGCCCGGTCATACGGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccc < 7:252483/132‑1 (MQ=255) tACTTACGTTGACGAGCC‑GGGCCCGGTCATACCGGGCACGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccc < 7:101538/135‑1 (MQ=255) tACTGACGTGGCCGAGCC‑GGGCCGGGTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccc < 7:11881/135‑1 (MQ=255) tACTGAAGTGCACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccct < 3:322852/136‑1 (MQ=255) acgTTGCCGCGCC‑GGGCCGGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccctgccc < 7:217237/133‑1 (MQ=255) aaGTTCACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccctgccc < 8:34068/135‑1 (MQ=255) gcccc‑gggccgggTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAacac < 1:17901/133‑1 (MQ=255) | CATCTCCTACCAGGGCACCGGTCTTTGCCTGCGG‑AACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCC‑GGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACAC > ACB143/4772412‑4772668 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |