Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,772,546 T→G 45.0% intergenic (‑140/+21) grsB ← / ← lgrB_1 Gramicidin S synthase 2/Linear gramicidin synthase subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,772,5460TG45.0% ‑3.5 / 34.7 86intergenic (‑140/+21)grsB/lgrB_1Gramicidin S synthase 2/Linear gramicidin synthase subunit B
Reads supporting (aligned to +/- strand):  ref base T (3/42);  new base G (31/3);  total (36/50)
Fisher's exact test for biased strand distribution p-value = 3.37e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

CATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACAC  >  ACB143/4772412‑4772668
                                                                                                                                        |                                                                                                                          
cATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGt                                                                                                                            >  2:97454/1‑135 (MQ=255)
cATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGt                                                                                                                            >  3:242729/1‑135 (MQ=255)
      cTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCGGGCATagcc                                                                                                                     >  5:125432/1‑133 (MQ=255)
      cTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGCGTACCg                                                                                                                     >  4:341766/1‑136 (MQ=255)
      cTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCCGGCATaacc                                                                                                                     >  7:299817/1‑133 (MQ=255)
      cTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCCGGCATAACg                                                                                                                     >  4:331883/1‑136 (MQ=255)
      cTACCAGGGCACCGGTATTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGTGCCGGGCATaacc                                                                                                                     >  7:276163/1‑133 (MQ=255)
        aCCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGCAAACCcgg                                                                                                                   >  1:135259/1‑136 (MQ=255)
           gggccACCGGTCTTTGCCTGCGGAACGTGCCCGCCCGCGACAGGCTCATCTTGCTGCCTAAAAAATGAACGCGCCGGACGGCTGCCGGCTCTGGGTGTACTGACGTTGACGACCCGGGCCCGGCCATACCGGGccc                                                                                                                <  8:315622/132‑1 (MQ=255)
           cgggCACCGGGCTTTGCCTGCGGACGGTCCCAGCCCGCGACAGGCTCTGGATCCTTCCCAAAAAATCAACGAGCCGGACGGCGGCCGGCTCTGGGTGTACTCAAGTTGACGAGCCGGGCCCGGTAATACCGGGccc                                                                                                                <  2:305632/135‑1 (MQ=255)
           aGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCGGGCCTACCGGGccc                                                                                                                >  5:157364/1‑136 (MQ=255)
           aGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGccc                                                                                                                <  3:331883/136‑1 (MQ=255)
              gccccGGTCTTTCCCTGCGGACCGTGCCAGCCCGCGACAGGCTCATGTTGCTGCCGAACAAATGAAAGACCCGGACGGCTGCCGGCTGTGGGTGTACTCAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCa                                                                                                             <  6:177281/133‑1 (MQ=255)
              gccccGGTCTTTCCCTCCGCAACGTCCCAGCCCGCGACAGGCTCATGATCCTCCCCAACAAATCAAAGCCCCGCACGGCGGCCGGCTCTGGGTGTACTCAAGTTGACGAGCCGGGCCCGGTAATACCGGGCCCGCa                                                                                                             <  6:129206/133‑1 (MQ=255)
              gCACCGGGTTTTGCCTGCGAACCGTGCCCGCCCGCAACAGGCTCATGATCCTCCCGAACAAAGGAACCAGCGGGCCGGCTGCCGTCTCTGTGTGTAGTGAAGTTGAGGAGCCGGGCCCGGTCATACCGGGCCCGCa                                                                                                             <  8:177616/136‑1 (MQ=255)
              gCACCGGGCTTTGCCGGCGGAACGGGCCAGCCCGCGACAGGCGCATGAGGCTGCCGACCAACTGACCGCGCCGGACGGCTGCCGGCTCTGGGGGTCCGGACGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCa                                                                                                             <  6:56544/136‑1 (MQ=255)
                aacGGTCTTTGCCTGCGGAAAGTTCCAGCCCGCGACAGGATCATGATGCTGCCGAACAAATGAAAGAGACGGACGGCTTCCGTCTATGGGTGTAATGAAGTTGACGAGCCGGGGCCGGTGAGAACGGGacccg                                                                                                              >  7:287746/3‑131 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGGCTCACCGGGccccccg                                                                                                            >  6:20421/1‑132 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGGCGGACCGGcccccc                                                                                                              >  2:17901/1‑132 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGCAATACCGGCCCGCcga                                                                                                           >  3:156912/1‑136 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCGGGGATACCCGGccccccca                                                                                                           >  5:160281/1‑131 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGGATAACCGGccccccgc                                                                                                           >  7:42225/1‑131 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGCATACCGGGCCCGccgc                                                                                                           >  5:364272/1‑133 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGGCTACCGGGccccccga                                                                                                           >  2:243425/1‑131 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGGCTACCCGGCCCGccgc                                                                                                           >  2:278982/1‑133 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGGATACCGGGCCCGCagc                                                                                                           >  7:101339/1‑135 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGCCATCCGGGcccccc                                                                                                             >  7:110406/1‑131 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGCATAACCGGCCCGCa                                                                                                             >  7:68434/1‑134 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCCGGGCTACCGGGCCCGccgc                                                                                                           >  6:156738/1‑133 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCCGGGATACCCGGcccccccc                                                                                                           >  1:96402/1‑131 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCCGGCCAACCCGGcccccccc                                                                                                           >  8:217237/1‑131 (MQ=255)
                aCCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCAGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGCATAGCGGGCCCCCagc                                                                                                           >  2:302615/1‑135 (MQ=255)
                   gggcTTTGCCTGCGCAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGCGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAgcg                                                                                                        <  7:243693/133‑1 (MQ=255)
                   ggTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGGATACCGGGCCCCCCGAgcg                                                                                                        >  2:15768/1‑136 (MQ=255)
                   ggTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGGAGAACGGGGCCGCcgcgcg                                                                                                        >  1:13751/1‑136 (MQ=255)
                   ggTCTTTGCCGGCGCACCGGGCCAGCCCGCGACAGGCTCATGATGCTGCCTAACAAATCAAAGCGCCGGCCGGCTGCGGGCTGTGGGTGTACTCACGTTCACGAGCCGGGCCCGGTCAAACCGGGCCCGCAGAgcg                                                                                                        <  8:108524/136‑1 (MQ=255)
                   ggTCTTTGCCGCGGCACCGGCCCCGCCCGCGACAGGCTCATGATGCTGCCGAACAAATCAAAGCGCCGCCCGGCGGCCGGCTGTGGGTGTACTGAAGTTGACGAGCCGGGCCGGGTAATCCCGGGCCCGCAGAgcg                                                                                                        <  8:76752/136‑1 (MQ=255)
                   ggTCTTGCCCTGCGCAACGTCCCCCCCCGCGACAGGCCCAGGAGCCGGCCAAACAAATGAACCCCCCGCACGGCTGCCGGCTCTGGGGTTACTGACGTTGCCGCGCCGGGCCCGGTAATACCGGGCCCGCAGAgcg                                                                                                        <  6:269121/136‑1 (MQ=255)
                    gTCTTTCCCGGCGGACCGTGCCAGCCGGCGACAGGCTCTTCATGCTGCCCAACAAATGACAGCGCCGGCCGGCTGCCGGCTCTGGGGGTCCTTAAGTAGACGCGCCGGGCCCGGTCAAACCGGGCCCGCAGAgcgc                                                                                                       <  7:67804/136‑1 (MQ=255)
                      cattGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCCGCCAGAGCGGGCGCCCAGAccgc                                                                                                       >  3:94565/3‑134 (MQ=255)
                       tttGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCCGGCAAGCCCGGCCCGCcgcgcgggt                                                                                                     >  6:273465/1‑132 (MQ=255)
                            cTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGCCCGGGATAACCGGCCCCCCCAGCGCGACggggg                                                                                               >  1:11546/1‑135 (MQ=255)
                                 gtaccGTGCCCGCCGGCGACTGGCTCTTGATGCTCCCAAACAAAGGAACGCGCGGGCGGGCGGCGGGCTCTGGGTGTACTGACGTTGACGACCGGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAAc                                                                                           <  6:186366/132‑1 (MQ=255)
                                   aaCGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGGGCGGGGGTACAGGGCCCCCCGCGCGCGGCGGGGTCGAc                                                                                           >  7:349190/1‑134 (MQ=255)
                                   aaCGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATTACAGGCCCGCAGAGCCCTAATGGGGGAACtc                                                                                         >  8:128890/1‑136 (MQ=255)
                                    aCGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcg                                                                                        <  7:226236/136‑1 (MQ=255)
                                      gggCCAGCCCGCTACAGGTTCATTATGCTGCCTAAAAAATGAAAGAGCGGGACGGGTGCCGGCTCTGGGTTTACTGAAGTTCACGAGCCGGGCCGGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc                                                                                      <  3:344996/134‑1 (MQ=255)
                                      gTGCCCCCCCGCGACAGGCTCATGAGGATGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGCCGCGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc                                                                                      <  6:224576/136‑1 (MQ=255)
                                      gTGCCACCCCGCCACCGGCTCTTTATGCTCCCCAACAAATCAAAGCGCCGGCGGGCTGCCGGCTGGGGGTGTCCTCACGTTCACGCCCCGGGCCCGGGCATCCCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc                                                                                      <  8:160900/136‑1 (MQ=255)
                                       gcccggCCCGCGACAGGCTCATGATGCGGCCGAACAAATGAAAGAGCCGGACGGCGGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc                                                                                      <  7:294183/130‑1 (MQ=255)
                                        cccAGCCCGCGACAGGCTCTTGATGCTGCGGAACAAATGAAAGACCCGGACGGCTGCCGGCTCTGGGTGTACTGACGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACtcgtc                                                                                      <  8:79791/133‑1 (MQ=255)
                                          cAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGGATACCGGGCCCCCCGCGCGCGACTGGGGGAACTCGTCGAtc                                                                                  >  1:243019/1‑136 (MQ=255)
                                           cgCCGGCGACAGGCTCTTGATGCTCCCTAAAAATTCAAAGCGCCGCAGGGGTGCGGGCTGTGGGTTTCCTTATGTGGACTCGCCGGGCCCGGAAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGAtct                                                                                 <  2:16589/135‑1 (MQ=255)
                                           cgCCCGCGACAGGCTCATGATGCTGCCGAACAAATCAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGAtct                                                                                 <  1:69658/135‑1 (MQ=255)
                                                      ggCTCATGTTGCGGCCTAAAAATTCAACGCGCGGGAGGGGTGCCGGGTGTGGGTTTACTGACGATGCCGCCCCGGGCCCGTTCATACGGGCCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGt                                                                       <  4:163258/135‑1 (MQ=255)
                                                                     ctaaCAAATGAACGCGCCGGACGGCTGCGGGCTCTGGGTGTACTTAAGTTGACGCCCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcg                                                       <  6:366786/134‑1 (MQ=255)
                                                                     cGAACAAATTAACGCCCCGGACGGCGGCGGGGTCTGGGTGTACTGAAGTTGACCAGCCGGGCCGGGTCATCCCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcg                                                       <  7:108803/136‑1 (MQ=255)
                                                                     cGAACAAATGAACGAGCCGGCCGGGGGCGGGCTCTGGGGGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcg                                                       <  8:30858/136‑1 (MQ=255)
                                                                     cGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGGATACCGGGCCCCCCGAGCGCGACTTGGGGAACTCGGAGATGTCCGCTGCAGTTAGAAGGTGCAAggcg                                                       >  3:156689/1‑136 (MQ=255)
                                                                      gAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTGAACTAGGAGATATACTCTGCAGTTATACGTTTAAAtgcgg                                                      >  6:115106/1‑136 (MQ=255)
                                                                      gAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGGCATACCGGGCCCGCCGACCGCGACGGGGTGAACTACTGGCTCTACGCTGGAGGAAGACGTTTGAAggcgg                                                      >  4:151098/1‑136 (MQ=255)
                                                                      gAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCCGGCAGAACGGGCCCGCCGAGCGCGACAGGGGGACCTCGTCGAGCGCCACTGGACTAAGACGGTTGAAggcgg                                                      >  2:126833/1‑136 (MQ=255)
                                                                        aaaattGAACGCGCCGGAGGGCTGCGGGCTCTGGGGTTACTCACGTGCCCGAGCGGGGCCCGGTAATACGGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAggcgg                                                      <  4:130920/129‑1 (MQ=255)
                                                                        aCAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCCGGGCTACCCGGCCCCCACCCCCCGACTGGGGGCACGCGGCGCTCGCCGCGGCCGTAAGCCGGTTAAAGGCGGcc                                                    >  7:125397/1‑136 (MQ=255)
                                                                                    gCCGGAGGGGGGCCGGCTCTGGGTTCACTTAAGTTCCCGCGCGGGGCCCGGTCATCCCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGcc                                        <  2:135214/136‑1 (MQ=255)
                                                                                             cTGCCGGCTCTGGGTGTACTGAAGTGGACGCGCCGGGCCCGGTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTcc                               <  1:63899/136‑1 (MQ=255)
                                                                                                  ggCTCTGGGTGTACTGAAGTTGACGCGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAAt                          <  1:3778/136‑1 (MQ=255)
                                                                                                  ggCTCTGGGTGTACTGAAGTTGACGCGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAAt                          <  4:93543/136‑1 (MQ=255)
                                                                                                  ggCGCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAAt                          <  7:123907/136‑1 (MQ=255)
                                                                                                   gcgcggggTGAACTTAAGTGGACGACCCGGGCCCGGAAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATc                         <  4:166478/131‑1 (MQ=255)
                                                                                                   gCTCTGGGTGTACTGAAGTTTACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGAAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATc                         <  6:289641/136‑1 (MQ=255)
                                                                                                        ggggGTACTGAAGTTGCGGCGCCGGGCCGGGCAATCCGGGCCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc                    <  8:158194/136‑1 (MQ=255)
                                                                                                        gggTGTACGCAAGTTGACGCCCCGGGCCCGGTAATACCGGCCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc                    <  6:357578/136‑1 (MQ=255)
                                                                                                         ggtcaaCTGAAGTTGACGACCCGGCCCCGTTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc                    <  7:288584/130‑1 (MQ=255)
                                                                                                         ggTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGAcgc                    <  5:156738/135‑1 (MQ=255)
                                                                                                          ttgtACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc                  <  8:207441/135‑1 (MQ=255)
                                                                                                          gttcaCTGAAGTTGCCGCGCCGGGCCCGGCCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc                  <  8:36110/132‑1 (MQ=255)
                                                                                                          gtgtAGTGAAGTTGACGAGCCGGGCCCGGTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc                  <  7:353134/136‑1 (MQ=255)
                                                                                                          gtgtACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc                  <  7:300780/136‑1 (MQ=255)
                                                                                                          gtgtACTCAAGTTGCCGAGCCGGGCCCGGGCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACgcgc                  <  8:212245/136‑1 (MQ=255)
                                                                                                            ttACTGAAGTGGCCGAGCCGGGCCGGGTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccc                <  1:128251/135‑1 (MQ=255)
                                                                                                            gcagtGAAGTTGACGCGCCGGGCCCGGTCATACGGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccc                <  7:252483/132‑1 (MQ=255)
                                                                                                             tACTTACGTTGACGAGCCGGGCCCGGTCATACCGGGCACGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccc                <  7:101538/135‑1 (MQ=255)
                                                                                                             tACTGACGTGGCCGAGCCGGGCCGGGTAATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccc                <  7:11881/135‑1 (MQ=255)
                                                                                                             tACTGAAGTGCACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccct               <  3:322852/136‑1 (MQ=255)
                                                                                                                  acgTTGCCGCGCCGGGCCGGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccctgccc           <  7:217237/133‑1 (MQ=255)
                                                                                                                  aaGTTCACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCgccctgccc           <  8:34068/135‑1 (MQ=255)
                                                                                                                          gccccgggccgggTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAacac  <  1:17901/133‑1 (MQ=255)
                                                                                                                                        |                                                                                                                          
CATCTCCTACCAGGGCACCGGTCTTTGCCTGCGGAACGTGCCAGCCCGCGACAGGCTCATGATGCTGCCGAACAAATGAAAGAGCCGGACGGCTGCCGGCTCTGGGTGTACTGAAGTTGACGAGCCGGGCCCGGTCATACCGGGCCCGCAGAGCGCTACTGGGTCAACTCGTCGATCTCCTCTGCAGTAAGACGTTTCAAGGCGGCCAAGGATTTAGCCAACTCGTCCTGAATCGACGCGCCCTGCCCTTGCAACAC  >  ACB143/4772412‑4772668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: