Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,788,943 T→G 8.6% S228R (AGC→CGC)  dat ← Diaminobutyrate‑‑2‑oxoglutarateaminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,788,9430TG 8.6% 750.5 / 3.0 349S228R (AGC→CGC) datDiaminobutyrate‑‑2‑oxoglutarateaminotransferase
Reads supporting (aligned to +/- strand):  ref base T (171/128);  new base G (39/2);  total (219/130)
Fisher's exact test for biased strand distribution p-value = 4.96e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 351 total aligned reads displayed.

GTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCAAGGCCCC  >  ACB143/4788809‑4789075
                                                                                                                                       |                                                                                                                                     
  cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTc                                                                                                                                    >  6:346228/1‑136 (MQ=255)
  cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTc                                                                                                                                    >  4:66632/1‑136 (MQ=255)
   gacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTcc                                                                                                                                   >  4:397457/1‑136 (MQ=255)
   gacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTcc                                                                                                                                   <  8:311380/136‑1 (MQ=255)
   gacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTcc                                                                                                                                   >  1:350911/1‑136 (MQ=255)
    acgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCg                                                                                                                                  <  2:293630/136‑1 (MQ=255)
       aTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGAt                                                                                                                               >  1:362763/1‑136 (MQ=255)
              ccccgcccgcgTGCTCGTTAATGCGCCGCACGCCTTTGTCCAATTCGATGGCGGCCGCAACCACCCCGCCCCCACCCTCCACCACTTCGAGGATCCCGGCGGGGGGCTGCGGCACGCCGCTTTCCGGATCGagcag                                                                                                                        <  4:225507/133‑1 (MQ=255)
                   cccgccTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCCCCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGt                                                                                                                   <  7:240723/136‑1 (MQ=255)
                   cccgccGGCTCGGTAATGCGCCGCACGCCATTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGt                                                                                                                   <  8:353617/136‑1 (MQ=255)
                    ccgccTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGtt                                                                                                                  >  7:187332/1‑136 (MQ=255)
                          gCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAg                                                                                                            >  3:67453/1‑136 (MQ=255)
                                  aTGCGCCGCAGGCCGTTTTGCCATTCGATGTCGGCCGGAACCACCCCGCGCTCACCGTGAACCACTTCGATGATCACGGCGGCGTGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGc                                                                                                     <  2:321123/135‑1 (MQ=255)
                                  aTGCGCCGCACGCCCTTGAGCAATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa                                                                                                    <  1:289196/136‑1 (MQ=255)
                                  aTGCGCCGAACGCCTTTCAGCCATTCGATGTCGGCGGTAACCACCCCGCCCTCACCTTGAACCAGTTGGTGTTGCACGGGGGGGGGCTGCGGCAGGGCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa                                                                                                    <  3:290844/136‑1 (MQ=255)
                                      gccgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTACGGCTCGAGCAGCAGGTTTTGCAGGGAATGCAGGtt                                                                                                >  3:53096/1‑136 (MQ=255)
                                        cgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGGTTTCCAGGTAATGCAGGTTgg                                                                                              >  2:134717/1‑136 (MQ=255)
                                        cgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGCTCGAGCCGCAGGTTTTGCAGGTAATGCAGGTTgg                                                                                              >  3:215696/1‑136 (MQ=255)
                                           aCGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCt                                                                                           >  6:76347/1‑136 (MQ=255)
                                           aCGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCt                                                                                           >  3:306901/1‑136 (MQ=255)
                                           aCGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCt                                                                                           >  7:268278/1‑136 (MQ=255)
                                           aCGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGAGCGAGCAGCAGGTTTTACAGGTAATGCAGGTTGGCCt                                                                                           >  1:309959/1‑136 (MQ=255)
                                             gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCCCCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCCGCCCGCCGCTTTCGGGATCGAGCAGCAGGTGTTCCAGGTAATTAAGGTTGGCCtt                                                                                          >  7:255228/1‑135 (MQ=255)
                                             gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTGCGGATCGTGCAGCAGGGTTTGCAGGTAATGCCGGTTgggcgtg                                                                                         >  1:185238/1‑131 (MQ=255)
                                             gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGCTCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCtt                                                                                          >  7:74990/1‑135 (MQ=255)
                                             gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGCTCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCtt                                                                                          >  3:358142/1‑135 (MQ=255)
                                             gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCtt                                                                                          >  2:230353/1‑135 (MQ=255)
                                             gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCtt                                                                                          >  1:108074/1‑135 (MQ=255)
                                             gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGGTTTCCAGGTAATGCAGGTTGGCCTTg                                                                                         >  4:281257/1‑136 (MQ=255)
                                             gCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTCCACCACTTCGAGGATCACGGAGCCGGGCACCCGCCCGCCCGTTTGCCGGAAGGGCAGCAGGTTTTGCATGTAATTCAAGTTgggcatg                                                                                         >  2:398669/1‑131 (MQ=255)
                                                  tGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCCGCACGCCGCATTCCGGTACGAGCAGCAGCGTTTCCAGGTAAAGCACGTTGGCCTTTAcccc                                                                                    >  5:58954/1‑136 (MQ=255)
                                                      ccaggcGATGTCGGCCGTAAGCACCCCGCCCTCACCCTGCACCACTTCGAGGATCCCGGCGGCGGGCAGCGGCAAGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGcc                                                                                <  7:110652/131‑1 (MQ=255)
                                                      ccATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGcc                                                                                <  2:268794/136‑1 (MQ=255)
                                                       cATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCCGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCt                                                                               >  7:89988/1‑136 (MQ=255)
                                                            gATGTCGGCCGTACCCACCCCGCCCTCACCCTGCCCCCCTTCGTGGACCACGGCGGCGGGCAGCGGCACGCGGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCAcc                                                                           <  5:94597/135‑1 (MQ=255)
                                                            gATGTCGGCCGGACCCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  4:355562/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGTTCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  4:250456/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  2:10870/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGGAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  7:212853/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGATCGAGCAGCAGGTTTTGCAGGTAATTCAGGTTGGCCTTGAACCCGGCCTCAccc                                                                          >  5:337358/1‑135 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCAGTGCCGATCGAGCACCACGTTTTGCATGGAATGCAGGTTGGGCTTGGCCCCCGCCTCAccc                                                                          >  6:228876/1‑135 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGTTCGAGCAGCAGGTTTTCCAGGTAATGGAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  1:37716/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  6:154341/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  1:248633/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCCCGTTGCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCCCCg                                                                          >  4:196156/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCCCGTTCCGGATCGAGCAGCAGGTTTTACAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  2:164802/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGAGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  8:23348/1‑136 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCACCGGCACCCCGCTTTCCCGATAGAGCAGCAGCTTTTACAGGTAAAGCAAGTTAGCCATGACCCCCGCCACAccc                                                                          >  6:22134/1‑135 (MQ=255)
                                                            gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAACGGCACGCCGCTTTCCGGATCGAGCAGCACGTTTTACAGGTTATGCAGGGTGGCCATGACCCCCGCcacaacc                                                                          >  3:132029/1‑133 (MQ=255)
                                                            gATGTCGGCCGGAACCACCACGCCCTCCCCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTGTCCGGATCGAGCAGCAGGTTTTCCAGGTAATTCAGGTTGGCCTTGACCCCGGCCTCACCg                                                                          >  8:268752/1‑136 (MQ=255)
                                                            gATGGCGGCCGGAACCCCCCCGCCCTCACCCTGCACCACTTCGCGGAACCGGGCGGGGGGCCGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCAcc                                                                           <  4:368491/135‑1 (MQ=255)
                                                                 cGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAg                                                                     >  7:42409/1‑136 (MQ=255)
                                                                      ggAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGGTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCCGGCCg                                                                 >  3:115686/1‑135 (MQ=255)
                                                                      ggAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCCCAGTGCGGATCGAGCAGCAGGTTTTGCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGccca                                                                >  8:161138/1‑134 (MQ=255)
                                                                           caccCCGCCCTCACCCTCCCCCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACggg                                                           <  7:365798/136‑1 (MQ=255)
                                                                              cccGCCCTCACCCTGCCCCCCTGCGCGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAg                                                        <  4:110504/136‑1 (MQ=255)
                                                                               ccGCCCTCACCCTGCACCCCTTCGCGGAGCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGc                                                       <  8:254553/136‑1 (MQ=255)
                                                                               ccGCCCTCACCCTGCACCACTTCGAGTAGCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGc                                                       <  6:380228/136‑1 (MQ=255)
                                                                                    cTCCCCCTGCACCACTTCGAGGAGCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAg                                                  <  5:148736/136‑1 (MQ=255)
                                                                                      cccccTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                                <  3:397457/134‑1 (MQ=255)
                                                                                      cACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                                <  6:163067/136‑1 (MQ=255)
                                                                                       ccccTCCCCCACTCCGCGGAGCCCGGCGGCGGGCCGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGt                                                 <  3:33284/133‑1 (MQ=255)
                                                                                       aCCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGCGCAGCAGGGTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCCAACGGGCAGCGGGTGTCg                                               >  5:249091/1‑136 (MQ=255)
                                                                                       aCCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                                <  2:7458/135‑1 (MQ=255)
                                                                                       aCCCTGCACCACTTCGAGGAGCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGt                                                 <  6:33351/134‑1 (MQ=255)
                                                                                             caccacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                          <  6:392415/134‑1 (MQ=255)
                                                                                             caccacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                          <  8:106020/134‑1 (MQ=255)
                                                                                             caccacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg                                          <  1:140374/134‑1 (MQ=255)
                                                                                                cacTTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                                       >  1:328735/1‑134 (MQ=255)
                                                                                                cacTTCGAGGATCACGGCGGCGGGCCCCGGCCCGCCCCTATCAGGATCTCGCAGCAGGTTTTGCATGTAAAGCAGGGTTGCCATGTCCCCCGCCGCCACCCCCACGCCGCACCGGCCGCGCGAGTCCTACCGGGTa                                      >  6:202718/1‑136 (MQ=255)
                                                                                                cacTTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCCGGCCGAACGGGCCGCGGTAGTCGTACGGGTaa                                     >  5:279333/1‑136 (MQ=255)
                                                                                                cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                                       >  6:265145/1‑134 (MQ=255)
                                                                                                cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                                       >  1:212865/1‑134 (MQ=255)
                                                                                                cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                                       >  5:195602/1‑134 (MQ=255)
                                                                                                cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                                       >  5:175974/1‑134 (MQ=255)
                                                                                                cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTaa                                     <  1:230353/136‑1 (MQ=255)
                                                                                                cacTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCACGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGt                                       >  2:253516/1‑134 (MQ=255)
                                                                                                   ttCGAGGATCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGc                                  <  5:353179/136‑1 (MQ=255)
                                                                                                   ttCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAgg                                   >  5:109375/1‑135 (MQ=255)
                                                                                                    tCGAGGATCACGGCGGCGGGCAGCGGCCCCCCGCATTACGGATCGAGCAGCAGGTTTTGCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAggg                                  >  6:19015/1‑134 (MQ=255)
                                                                                                    tCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCCGGCCGCACGGGCAGCGGTAGGCGTACGGGTAAGGCa                                 >  8:187717/1‑136 (MQ=255)
                                                                                                    tCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCa                                 >  4:174850/1‑136 (MQ=255)
                                                                                                    tCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGACTTGACCCCGGCCTCACCGCCCAGGCCGAAAGGCCAGCGGTAGTCGTACGGGTAAGGCa                                 >  4:343653/1‑136 (MQ=255)
                                                                                                     cGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGt                                                                                                                   <  8:74720/54‑1 (MQ=255)
                                                                                                     cGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt                                >  3:247987/1‑136 (MQ=255)
                                                                                                     cGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt                                >  2:93785/1‑136 (MQ=255)
                                                                                                     cGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCATGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt                                >  3:152935/1‑136 (MQ=255)
                                                                                                     cGAGGATCACGGCGGCGGGCAGCGCCACGCCGCATAACGGAACGACCACCCGGTTTTCCAGGTAATGCCAGTTGGCCATGACCCCCGCCGAACCGCCCACGCCGCAACCGCACCGGTAATAGTACCGGTAAAGCaa                                >  2:190980/1‑135 (MQ=255)
                                                                                                      gAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                                <  5:295524/120‑1 (MQ=255)
                                                                                                      gAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc                                                >  6:295524/1‑120 (MQ=255)
                                                                                                      gAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt                                >  7:134222/1‑135 (MQ=255)
                                                                                                          cacccgggggcggGCAGGGCCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACt                           <  2:354510/132‑1 (MQ=255)
                                                                                                               ggcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACg                      <  6:809/136‑1 (MQ=255)
                                                                                                                gcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGc                     >  2:62162/1‑136 (MQ=255)
                                                                                                                gcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGc                     >  8:14696/1‑136 (MQ=255)
                                                                                                                       gCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCCGGCCGAACGGGCAGCGGGAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGcc              >  7:332285/1‑136 (MQ=255)
                                                                                                                         aGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGa            <  6:116977/136‑1 (MQ=255)
                                                                                                                         aGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGa            >  2:371414/1‑136 (MQ=255)
                                                                                                                            ggaccgccgcTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCa         <  8:213321/132‑1 (MQ=255)
                                                                                                                                    cgcTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCAAGGcccc  >  4:143452/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GTCGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCACTTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTGACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCAAGGCCCC  >  ACB143/4788809‑4789075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: