BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	4,799,383	G→C	7.2%	S550C (TCC→TGC)	CJ019_04509 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,799,383	0	G	C	7.2%	673.0 / 4.7	203	S550C (TCC→TGC)	CJ019_04509	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (136/39); new base C (2/14); total (138/65)
Fisher's exact test for biased strand distribution p-value = 3.00e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

TCGGCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGTCGAGGAT  >  ACB143/4799248‑4799516

tcgGCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCgg                                                                                                                                       <  7:45960/136‑1 (MQ=255)

tcgGCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCgg                                                                                                                                       <  7:89143/136‑1 (MQ=255)

tcgGCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCgg                                                                                                                                       <  3:223683/136‑1 (MQ=255)

  ggCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGaa                                                                                                                                     >  8:15442/1‑136 (MQ=255)

  ggCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGaa                                                                                                                                     >  6:160875/1‑136 (MQ=255)

  ggCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGaa                                                                                                                                     >  4:184613/1‑136 (MQ=255)

  ggCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGaa                                                                                                                                     >  1:383636/1‑136 (MQ=255)

   gCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAAc                                                                                                                                    <  6:349307/136‑1 (MQ=255)

   gCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAAc                                                                                                                                    <  7:370647/136‑1 (MQ=255)

     cGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCt                                                                                                                                  <  1:98307/136‑1 (MQ=255)

        aCGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTa                                                                                                                               <  3:374807/136‑1 (MQ=255)

          ggCCGAGGAAATCGCTGACCAGCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCTATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTAcc                                                                                                                             <  2:270133/136‑1 (MQ=255)

          ggCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGTTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTAcc                                                                                                                             <  7:294311/136‑1 (MQ=255)

          ggCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACCTGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTAcc                                                                                                                             <  5:62910/136‑1 (MQ=255)

          ggCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTAcc                                                                                                                             <  5:50748/136‑1 (MQ=255)

                       gCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTg                                                                                                                 <  6:36448/135‑1 (MQ=255)

                         tGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTgg                                                                                                                >  2:295688/1‑134 (MQ=255)

                              accaAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATgg                                                                                                         >  1:100761/1‑136 (MQ=255)

                                        gggAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGAc                                                                                               >  4:143296/1‑136 (MQ=255)

                                         ggAGGTGGACATGCGGGGGCCCCGCGCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCAGCGGCGCCGCGGAACCTGTACCGAAGGCCTTCTGGCAGATGGCGACGCCGa                                                                                                <  6:379267/134‑1 (MQ=255)

                                         ggAGGGGCACATCCCGGGGCCCAGCGCGGGGCCGATAGGGCGCCGGCGTATGGGGCCATGGGGGCTTAACTCCACCCTGGCCATCGGCGCCTCGGAACCTGTACCTAAGTCCTTCTGGCAGTTGGCGAAGCCGACg                                                                                              <  8:196384/136‑1 (MQ=255)

                                          gAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGc                                                                                             >  7:234653/1‑136 (MQ=255)

                                          gAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGc                                                                                             >  3:170373/1‑136 (MQ=255)

                                            ggTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCg                                                                                           >  7:215558/1‑136 (MQ=255)

                                            ggTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCg                                                                                           >  1:304039/1‑136 (MQ=255)

                                            ggTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCg                                                                                           >  1:66820/1‑136 (MQ=255)

                                             gTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCg                                                                                           >  4:43022/1‑135 (MQ=255)

                                             gTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGCAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGccgccgccg                                                                                           >  8:183847/1‑135 (MQ=255)

                                                 aCATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCTGCCATCGGCGATGAACTCGACACTGGCCATCGGCGCCTAGGAACCTGTAACGAAGTCCTTCTGGCAGATGGCGAAGCAGACGCCGTAcc                                                                                       >  1:341026/1‑135 (MQ=255)

                                                 aCATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGACGCCGACGCCGTAc                                                                                        >  6:205934/1‑134 (MQ=255)

                                                 aCATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGCCGCCGTAc                                                                                        >  8:272268/1‑134 (MQ=255)

                                                 aCATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTAc                                                                                        >  1:388611/1‑134 (MQ=255)

                                                 aCATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTAc                                                                                        >  3:61132/1‑134 (MQ=255)

                                                 aCATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTAc                                                                                        >  2:229612/1‑134 (MQ=255)

                                                  cATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAg                                                                                     >  8:120014/1‑136 (MQ=255)

                                                  cATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAg                                                                                     >  1:198270/1‑136 (MQ=255)

                                                   aTGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGt                                                                                    >  1:184898/1‑136 (MQ=255)

                                                     gCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTgg                                                                                  >  7:332850/1‑136 (MQ=255)

                                                        ggTGCCCAGCTCGGTGCCGATATTGCGCAGGCTGATTCGGCCATCGGCGCGGAACTCGAACCTGGCCCTCGGCGCCTACGAACCTGTACCGAAGTCCATCTGGCAGATGGCGAAGCCGACGCCGGACCAGTGGTcc                                                                               >  8:278191/1‑136 (MQ=255)

                                                        ggTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTcc                                                                               >  3:119516/1‑136 (MQ=255)

                                                        ggTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTcc                                                                               >  2:382985/1‑136 (MQ=255)

                                                           gCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCggg                                                                            >  4:239498/1‑136 (MQ=255)

                                                             ccAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTc                                                                          >  6:194315/1‑136 (MQ=255)

                                                             ccAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTc                                                                          >  5:385472/1‑136 (MQ=255)

                                                                  ggggTGCCTATTTGCGGCAGGTTGTTGCGGCCATGGGGGTTTACCTCTAAAGTGCCAAGGGGCGCCTCGTACCCTTAACCTATGGCCTTCGGGCTGTGGGCCACCCCGACGCCGTACCAGTGGTCCGGGTCCTTgg                                                                     <  6:324865/134‑1 (MQ=255)

                                                                    ggTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCg                                                                   >  1:283805/1‑136 (MQ=255)

                                                                       gCCGATATGGCGCAGGCGGATGCGGCCAGCGGCGCTGAACTCGACACTGGCCAGCGGCGCCTCGCAACCTGTACCGAAGTCCTTCTGGCAGAGGGCGACGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTcc                                                                <  7:68695/136‑1 (MQ=255)

                                                                         cGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGCCGCCGTACCAGTGGTCCGGGTGCTTGGCGTCCAt                                                              >  3:236486/1‑136 (MQ=255)

                                                                         cGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCAt                                                              >  1:330602/1‑136 (MQ=255)

                                                                              tGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGACGTCCTGCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTGGGCGTCCATCTGtt                                                         >  8:329190/1‑136 (MQ=255)

                                                                                  gcAGGCTGTTGCGGCCATCGGCGCTTAACTCGACACTGCCCATCGGCGCCGCGCACCCTGTACCGAAGTCCTTCTGGCAGATGGCGACGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGttgctt                                                     <  7:120290/136‑1 (MQ=255)

                                                                                  gcAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGACATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGttgctt                                                     >  3:327769/1‑136 (MQ=255)

                                                                                   cAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGttgcttg                                                    >  4:254062/1‑136 (MQ=255)

                                                                                        tGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCgtg                                                >  4:282695/1‑135 (MQ=255)

                                                                                         gATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTa                                              >  3:140324/1‑136 (MQ=255)

                                                                                         gATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTa                                              >  8:225084/1‑136 (MQ=255)

                                                                                             cagCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATcgc                                          >  1:143632/3‑136 (MQ=255)

                                                                                             cGGCCATCGGCGCTGAACTCTACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATcgc                                          >  1:256376/1‑136 (MQ=255)

                                                                                                  aTCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTg                                     >  8:77756/1‑136 (MQ=255)

                                                                                                   tCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgc                                    >  1:242876/1‑136 (MQ=255)

                                                                                                   tCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgc                                    >  3:379401/1‑136 (MQ=255)

                                                                                                   tCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgc                                    >  5:13373/1‑136 (MQ=255)

                                                                                                   tCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgc                                    >  6:377962/1‑136 (MQ=255)

                                                                                                   tCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgc                                    >  5:211722/1‑136 (MQ=255)

                                                                                                   tCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgc                                    >  5:250917/1‑136 (MQ=255)

                                                                                                     ggggcTTAACGCCACACTGGCCAGGGGCGCCTCGCAACGTTAACCTAAGTCATTGTGGCAGATGGCTACCCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgcgc                                  <  6:130387/133‑1 (MQ=255)

                                                                                                     ggggcTGACCTCTCCACTGGCCATCGGCGCCTCGCAACCTTAACCTAAGTCTTTCTGGCATTGGGCGACGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgcgc                                  <  4:344185/133‑1 (MQ=255)

                                                                                                     ggCGGTTAACGCGACCGTGCCCATCGGCGCCGCGCACCGTGTCCCGAGGGCTTTCTGGCTTTGGGCGACGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgcgc                                  <  6:14335/136‑1 (MQ=255)

                                                                                                     ggCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGCCGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgcgc                                  >  6:71020/1‑136 (MQ=255)

                                                                                                     ggCGCTGAACTCGAAACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGGCCTGCTGGCAGAGGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgcgc                                  <  7:201372/136‑1 (MQ=255)

                                                                                                     ggCGCTCAACTCGACCCTGCCCAGCGGCGCCTCGCACCCTGACCCCATGTCCTTCTGGCAGAGGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgcga                                  <  2:90309/136‑2 (MQ=255)

                                                                                                     ggCGCGTAACACTACCCTGGCCAGCGGCGCCTCGTAACCTGACCCGAAGGCTTTTTGCCCGATGGCTACGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTgcgc                                  <  3:278669/136‑1 (MQ=255)

                                                                                                       cgcTGAACTCGACCCTGGCCATCGGCGCCTCGGAACCTGTCCCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGcca                                <  2:253527/136‑1 (MQ=255)

                                                                                                        gcTGAACGCGACACGGGCCATCGGCGCCTCGCACCCTGACCCGAAGCCCTTCTGCCAGAGGCCGACCCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGccac                               <  5:122531/136‑1 (MQ=255)

                                                                                                        gataaccTCGACCCGGGCCTTCGGCGCCTCGCACCCTTTACCTAAGTCATTCTGGCAGAGGGCTACCCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGccac                               <  4:365807/130‑1 (MQ=255)

                                                                                                             ccTCGACACGGGCCATCGGGGCCGGGGAACGTGAACCTATGGCTTGCTGGCATTTGGCTACGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTc                          <  6:334326/135‑1 (MQ=255)

                                                                                                             aCTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACt                           <  2:327017/135‑1 (MQ=255)

                                                                                                             aCTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTc                          >  6:279397/1‑136 (MQ=255)

                                                                                                               tCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGt                        >  4:67731/1‑136 (MQ=255)

                                                                                                               tCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCGGTTGCTTGCGTGTATCGCGGTTGCGCCCCCACTCGt                        >  6:232607/1‑136 (MQ=255)

                                                                                                                  aaacTGGCCATCGGCGCCTCGGAACCTGTACCTATGTCCTTGTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGca                     <  8:306503/134‑1 (MQ=255)

                                                                                                                    acTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGca                     >  8:103461/1‑134 (MQ=255)

                                                                                                                    acTGGCCATCGGCGCCTCGCAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCACTGCTCCGGGTCCTAGGCGTCCAACTGATGCTAGCGTGAATCGCGGTAGCGCCACCACTCCTGca                     >  3:302991/1‑134 (MQ=255)

                                                                                                                       ggCCATCGGCGCCTGGTACCCTGACCCCATGTCCTTCTGGCAGAGGGCTAACCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCg                <  4:135578/136‑1 (MQ=255)

                                                                                                                         ccATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGc              >  8:35393/1‑136 (MQ=255)

                                                                                                                         ccATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGc              >  1:210315/1‑136 (MQ=255)

                                                                                                                         ccATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGc              >  7:78916/1‑136 (MQ=255)

                                                                                                                         ccATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGc              >  2:54936/1‑136 (MQ=255)

                                                                                                                         ccATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGAGGTTGCGCCCCCACTCGTGCACAGCGGc              >  4:338245/1‑136 (MQ=255)

                                                                                                                           aTCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCt            >  4:214350/1‑136 (MQ=255)

                                                                                                                              ggCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGt         >  8:41956/1‑136 (MQ=255)

                                                                                                                              ggCGCCTCGGAACCTGTACCGAAGGCTTTGTGGCAGATGGCTAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGt         <  5:280013/136‑1 (MQ=255)

                                                                                                                                cgcCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGTc        >  2:134046/1‑135 (MQ=255)

                                                                                                                                  ccTGGGACCCTTTCCCCACGTCCTTCTGGCTTAGGGCCACGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGTCGAg     <  5:58502/136‑1 (MQ=255)

                                                                                                                                   cTCGGAACCTGTACCGAAGTCCTTCTGGCAGAGGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGTCGAgg    <  7:193739/136‑1 (MQ=255)

                                                                                                                                     cGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCCCTCGTGCACAGCGGCCTTGTCGAGGAt  >  7:151876/1‑136 (MQ=255)

                                                                                                                                     cGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGTCGAGGa   >  8:168732/1‑135 (MQ=255)

                                                                                                                                     cGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGTCGAGGa   >  3:73273/1‑135 (MQ=255)

                                                                                                                                     cGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGTCGAGGAt  >  5:380430/1‑136 (MQ=255)

                                                                                                                                      ggAACCTGAACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGTCGAGGa   <  6:388590/134‑1 (MQ=255)

TCGGCCGAACGGCCGAGGAAATCGCTGACCACCAAAGCCTGGGAGGTGGACATGCCGGTGCCCAGCTCGGTGCCGATATGGCGCAGGCTGATGCGGCCATCGGCGCTGAACTCGACACTGGCCATCGGCGCCTCGGAACCTGTACCGAAGTCCTTCTGGCAGATGGCGAAGCCGACGCCGTACCAGTGGTCCGGGTCCTTGGCGTCCATCTGTTGCTTGCGTGTATCGCGGTTGCGCCACCACTCGTGCACAGCGGCCTTGTCGAGGAT  >  ACB143/4799248‑4799516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`