Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,805,214 A→C 16.3% intergenic (+141/‑608) dsbG → / → CJ019_04513 Thiol:disulfide interchange protein DsbG/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,805,2140AC16.3% 298.6 / 4.6 147intergenic (+141/‑608)dsbG/CJ019_04513Thiol:disulfide interchange protein DsbG/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (74/40);  new base C (2/26);  total (76/71)
Fisher's exact test for biased strand distribution p-value = 1.55e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

TGGTGGGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTG  >  ACB143/4805080‑4805349
                                                                                                                                      |                                                                                                                                               
tggtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCaa                                                                                                                                                 <  5:190300/135‑1 (MQ=255)
 ggtggGGCACAGATCGAGCGCCTGGAATCAGGCGACGTGGGGCATGTCACCGGCTACTACGGTGTTCTCGGGTGAACCAGCTACTACGGGGCTCGCTCTAACGGCTGGGTTTTGCGACAGGCCCGTGATCGCAAgg                                                                                                                                               >  8:298714/1‑136 (MQ=255)
 ggtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCCTGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTACAGGCTGGGTTTTGCGCCAGGCCCGGGGTCGCAAgg                                                                                                                                               >  6:262007/1‑136 (MQ=37)
 ggtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAgg                                                                                                                                               >  8:196350/1‑136 (MQ=255)
 ggtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAgg                                                                                                                                               >  1:47877/1‑136 (MQ=255)
 ggtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAgg                                                                                                                                               >  3:318774/1‑136 (MQ=255)
 ggtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAgg                                                                                                                                               >  7:160575/1‑136 (MQ=255)
 ggtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAgg                                                                                                                                               >  4:210064/1‑136 (MQ=255)
 ggtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCCCTCCTACGGGGCGCGCGCTAACGGCTGGGTTTTTCGCCCGGCCCGTGATCGCAAgg                                                                                                                                               >  7:261215/1‑136 (MQ=255)
  gtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTATCGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGc                                                                                                                                              >  6:321746/1‑136 (MQ=255)
  gtggGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCCCGCCCACGGGGCGCGCGCTAACGGCGGGGTTTTGCGCCAGGCCCGGGATAGCAAGGc                                                                                                                                              >  3:64402/1‑136 (MQ=37)
    ggggCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCt                                                                                                                                            >  3:22939/1‑136 (MQ=255)
    ggggCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCt                                                                                                                                            >  1:327191/1‑136 (MQ=255)
    ggggCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCt                                                                                                                                            <  1:87248/136‑1 (MQ=255)
      ggCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTgc                                                                                                                                          >  2:194641/1‑136 (MQ=255)
       gCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTgcg                                                                                                                                         >  2:227016/1‑136 (MQ=255)
       gCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTgcg                                                                                                                                         <  6:266739/136‑1 (MQ=255)
       gCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTgcg                                                                                                                                         >  1:89742/1‑136 (MQ=255)
       gCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTgcg                                                                                                                                         <  6:97571/136‑1 (MQ=255)
       gCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTgcg                                                                                                                                         >  5:360033/1‑136 (MQ=255)
        cacaGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTgcgc                                                                                                                                        >  8:379358/1‑136 (MQ=255)
         acaGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGcgcg                                                                                                                                       <  2:4834/136‑1 (MQ=255)
         acaGATCGAGCGCCGGGACGCAGGCGACGTGTGGCAGGGCACCGGCTACGCCGGTTTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGcgcg                                                                                                                                       <  8:245633/136‑1 (MQ=38)
             aTCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCg                                                                                                                                   >  6:291255/1‑136 (MQ=255)
              tCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCgg                                                                                                                                  >  7:86842/1‑136 (MQ=255)
               cGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCCCCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCGGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGc                                                                                                                                 <  4:81517/136‑1 (MQ=255)
                gAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGcc                                                                                                                                >  5:124420/1‑136 (MQ=255)
                gAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGcc                                                                                                                                >  7:220946/1‑136 (MQ=255)
                gAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGcc                                                                                                                                >  6:196628/1‑136 (MQ=255)
                   cgcCGTGAAGCAGGCGACGGGGGGCAGGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTa                                                                                                                           <  7:324656/136‑1 (MQ=255)
                    gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGTACCCCCTGCTACGGGGCTCGCCCAAACCGCTGGGGTTTTCGCCCCGCCCCGGCTAGCAAGGCCAGCGCGGGCCGCCGGAg                                                                                                                          >  3:49061/1‑136 (MQ=255)
                    gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGcccttg                                                                                                                            >  5:83395/1‑132 (MQ=255)
                    gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGccctgg                                                                                                                            >  1:275415/1‑132 (MQ=255)
                    gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCggtg                                                                                                                            >  1:396059/1‑133 (MQ=255)
                    gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGttg                                                                                                                           >  6:293277/1‑134 (MQ=255)
                    gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGttg                                                                                                                           >  4:399762/1‑134 (MQ=255)
                    gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGtgg                                                                                                                           >  5:122572/1‑134 (MQ=255)
                    gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAg                                                                                                                          >  7:106918/1‑136 (MQ=255)
                    gcCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAg                                                                                                                          <  3:285279/136‑1 (MQ=255)
                    ccgggcaaGCAGGCGCCGGGGGGCATGGCACCGGCTACGCCGGTGGTCGCGGGCGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCGGCGCGGTCGGCCGTAg                                                                                                                          <  1:206470/130‑1 (MQ=255)
                     ccGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGTACCCGCACCGAAGGGGCTCGCGCTAACCGCTGGGTTTTGCGCCAGGCCCCTGCACGCCAGGCCTGCGCGGTCGGCCGttg                                                                                                                           >  5:354757/1‑133 (MQ=25)
                          aaGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGCgg                                                                                                                 >  6:72325/1‑136 (MQ=255)
                          aaGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTGGGGGCgg                                                                                                                 >  5:220371/1‑136 (MQ=255)
                                   aCGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTATGGGCGCGGGTTAccg                                                                                                         >  1:14677/1‑135 (MQ=38)
                                   aCGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGCGCGGGGTTTAccc                                                                                                         >  7:89914/1‑136 (MQ=255)
                                       ggggCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCCTAGGGGCGGGGTTTACCcccg                                                                                                      >  1:384078/1‑135 (MQ=38)
                                             tGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGCGGGGGTTACCGCCGAAGGGGc                                                                                               >  8:304955/1‑136 (MQ=37)
                                                 aCCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGCGCCGGGTTTACCCCCGCATGGCCCgcc                                                                                           >  6:162048/1‑134 (MQ=38)
                                                      cTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCCGCGCGGTCGGCCGTGGGGGGCGGGGTTGCCCCCCAAGGGCCCggccagcc                                                                                      >  6:118411/1‑130 (MQ=37)
                                                         cGCCGGTGTTCGCGGGGTAACCCGCTCCTACGGGGCTCGCGCAAACGGCTGGGTTTTGCGCCCGGCCCGTGAGCGCACGGCCGGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGc                                                                                <  5:293775/136‑1 (MQ=37)
                                                                 ttCGCGGGGGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTc                                                                         <  7:379339/135‑1 (MQ=255)
                                                                  tCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTgcg                                                                                                                                         >  5:233223/1‑77 (MQ=255)
                                                                  tCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTgcg                                                                                                                                         <  6:233223/77‑1 (MQ=255)
                                                                     cgGGTTAACCCGCTCCTACGGGGCGCGCGCTAACGGCGGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTccacca                                                                    <  7:195932/136‑1 (MQ=255)
                                                                        gttaccccGCTCCTACGGGGCGCGGGATACCGGGTGGGTTTTGCGCCCGGCCCGTTATGGCACGGCGTGCGGGGTGGGCCTTTGGTGGGGTTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGcc                                                                 <  7:73580/131‑1 (MQ=16)
                                                                        gttaaCCCGCTCCTACGGGGCGCGGGCTAAGGGGTGGGTTTTGCGCCTGGCCCGTTATCGCACGGCCTGCGCGGTCGGCCGTTGGTGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGcc                                                                 <  6:354757/133‑1 (MQ=21)
                                                                                    cTAGGGGGCTCGCGCTACCGGCTGGGTTTGGCGCCAGGCCCGTGAGCGCACGGCCGGCGCGGGCGGCCGTAGGCGGGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgc                                                     <  8:261215/136‑1 (MQ=21)
                                                                                       gggggCGCGCGCTACGGGCTGGGTTTTGCGCCTGCCCGGTTAGCGCACGGCGGGCGGGGTCGGCGGTAGGCGGGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgc                                                  <  3:355377/135‑1 (MQ=18)
                                                                                         gggCTCGGGCAAACGGCGGGGTTTTGCGCCAGGCCGGTCATCGCACGGCCTGCGCGGGCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgccg                                                <  6:181743/136‑1 (MQ=18)
                                                                                         gggCTCGCGCTACCGGCGGGGTTTTGCGCCAGGCCGGTGATCGCAAGGCCTGCGCGGGCGGCCGTAGGCGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgccg                                                <  7:259190/136‑1 (MQ=21)
                                                                                         gggCTCGCGCTACCGGCGGGGTTTTGCGCCAGGCCGGTGATCCCACGGCCTGCCGGGTCGGCCGTAGGAGGGGTTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgccg                                                <  6:438/136‑1 (MQ=21)
                                                                                         gggCGCGCGCTAACGGGTGGGTTTTGCGCCAGGCCCGTGATCGCACGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgccg                                                <  7:371438/136‑1 (MQ=255)
                                                                                         gggCGCGCCCTAAGGGCGGGGTTTTGCGCCAGGCCCGGTAGCGCACGGCGGGCGGGGTGGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTgccgccg                                                <  8:43353/136‑1 (MQ=18)
                                                                                          ggCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTGGGGGCCGGGGTTACCCCGCAAGGGGCGGGCCCGCGGGGCCAATTAACACCACCCGTTCCCCGGTgcccccc                                                >  5:111645/1‑135 (MQ=255)
                                                                                           gCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGGGCGGGGTTAACCGCGCAAGGGCCGGGCCGGCGGGGCCAATTCAACCAGCCGGTCACCCGTgccgccccc                                              >  1:251729/1‑136 (MQ=255)
                                                                                               gcgcTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGGGGGGTTTTCCCCGGAAGGGGCCGCCCGGGGGGGCCAATTTGCCCAGCCGGGGACCGGTggcgccggccccg                                          >  5:80154/1‑130 (MQ=255)
                                                                                               gcgcTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGCCGGGTTTACCGCCCAACGGGCGGGCCGGGGGGGCCAATTACCCCACCCGGGAACCCTTgccgcccccccag                                          >  5:256827/1‑132 (MQ=255)
                                                                                               gcgcTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGGTTACCCCCGAAAGGGCCCGCCCAGCTGGCCCATTTACCCCAGCCGGTCCCCGGTTCCCCCGCCCCTg                                          >  8:95058/1‑136 (MQ=255)
                                                                                               gcgcTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGGTTACCCCCCAAATGGGCGGCCCAGCGGGCCAATTTCCCCCCGCCGGTCCCCCTTgccgccgccccag                                          >  7:307852/1‑132 (MQ=21)
                                                                                                 gcTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTTGAGCGGGGTTTACCCCGCAAGGGGCGGCCCATCGGGGCAATTTTCACCCGGCGGTGACCGGTggcgccgccccagtt                                        >  7:220033/1‑130 (MQ=255)
                                                                                                 gcTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGCCGGGTTTACCCCGCAAGGGGCCGGCCGGGGGGGCCAATTTCCCCCGCCCGTGACCCGTTCCGCCGCCCCAGTa                                        >  2:211403/1‑136 (MQ=255)
                                                                                                 gcTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGCGCGGGGTTTACCCCGCAAGGGGCCGGCCCGCGGGGCCAATTGCCCCCGCCGGTGACCGGTgccgccgccccagtt                                        >  4:304833/1‑130 (MQ=255)
                                                                                                 gcTAACGGCGGGGTTTTGCGCCAGGCCGGTTATCGCACGGCCGGCGCGGTCGGCGGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTa                                        <  2:47952/136‑1 (MQ=18)
                                                                                                 gacagcGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTa                                        <  2:322679/131‑1 (MQ=255)
                                                                                                            tgTTTTGCGCCAGGCCGTTTTGCGAAAGGCCTGCGCGGTGGGCCGTAGGTGGGGTTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                            <  6:85619/135‑1 (MQ=14)
                                                                                                            ggTTTTGCGCCAGGCCGGTGAGCGCAAGGCCGGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                            <  4:287217/136‑1 (MQ=255)
                                                                                                            ggTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                            <  8:89059/136‑1 (MQ=255)
                                                                                                            ggTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                            <  8:312986/136‑1 (MQ=255)
                                                                                                            ggTTTTCCGACCGGACCGTGATCGCACGGCCTGCGCGGTCGACCGTAGGGGCGGTTTTACCAGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCATCCGTTCACCGTTGACGCCGCCACTGTATTCGCGGTTaa                            <  8:113411/136‑1 (MQ=17)
                                                                                                            ggTTTGGCCCCTGCCCCGTTATCCCACGGCCGGCGCGGTGGGCCTTAGGCGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa                            <  2:307684/136‑1 (MQ=17)
                                                                                                             gTTTTGCGCCTGGCCCGTGATCGCATGGCCTGCGCGGTCGGCCGTAGGAGGGGGTTTACCCGCGAAAGGGCGGGACGGGCTGGCAAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaaa                           <  7:188788/136‑1 (MQ=18)
                                                                                                             gTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCCGGTTTTCCCCCGAAAGGGCCCGGCCGGCGGGCCAATTTACACCCGCCCTTGACCCGTGCCGCCGCCACAGTATTAGCGGGGaa                            >  8:312917/1‑135 (MQ=255)
                                                                                                                ttGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGGGCGGGGTTTACCCCGCAAGGGGCGGCCCCGGGGGGCCAATTGCCCCCGCCGGTGACCGGTTGCGCCGCCCCTGTTTTAGCGGGGTAAAcc                         >  2:241283/1‑136 (MQ=255)
                                                                                                                    gcCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTa                                        <  7:153932/117‑1 (MQ=255)
                                                                                                                    gcCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTa                                        >  8:153932/1‑117 (MQ=255)
                                                                                                                      ccggCCGGTGATCTCACGGCCGGCGGGGCCGGCCGTAGGCGGGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCt                  <  3:329533/134‑1 (MQ=17)
                                                                                                                      ccggCCCGTGATCGCAAGGCCGGCGCGGTCGGCGGTCGGAGCGGTTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCt                  <  2:372510/134‑1 (MQ=17)
                                                                                                                      cAGGCCCGTGATCGCCCGGCCTGCGCGGGCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCt                  <  4:306195/136‑1 (MQ=255)
                                                                                                                      cAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCt                  <  2:292270/136‑1 (MQ=255)
                                                                                                                      cAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCt                  <  8:106918/136‑1 (MQ=255)
                                                                                                                      cAGGCCCGTGACCGCAAGCCCGCCGCGGGCGGCGTTCGCCGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGATCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCt                  <  5:100578/136‑1 (MQ=17)
                                                                                                                         gCCCGTGATCGCACGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACg               <  8:195716/136‑1 (MQ=255)
                                                                                                                           cggttaTCCCACGGCCTGCGCGTGCGGCGTTAGGATCGGTTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCTTTCACCGTTGCCTCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCt             <  8:157855/131‑1 (MQ=14)
                                                                                                                           ccGTGATCGCACGGCCGGCGCGGTCGGCCGTAGGCGGGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCt             <  7:75202/136‑1 (MQ=255)
                                                                                                                           ccGTCAGCGCACGGCCGGCGCGGTGGGCGGTAGCAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCt             <  3:278435/136‑1 (MQ=17)
                                                                                                                             gTGATCGCACGGCGGGCGGGGTCGGCCGTAGTAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGa           <  4:267657/136‑1 (MQ=17)
                                                                                                                                aTCGCACGGCCGGCGGGGGCGGCGGTAGCCGGGGTTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATc         <  2:89742/135‑1 (MQ=17)
                                                                                                                                 tCGCATGGCGGGCGGGGGGGGCGGTTGGCGGGGTTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCg        <  5:196628/135‑1 (MQ=14)
                                                                                                                                  cGCAAGGCCTGCGCGGTCGGCCGTAGGCGGGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCgg       <  6:358066/135‑1 (MQ=255)
                                                                                                                                    cAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCggggg    <  1:233371/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                               
TGGTGGGGCACAGATCGAGCGCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTG  >  ACB143/4805080‑4805349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: