Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,844,988 A→T 19.3% intergenic (+64/+55) apt → / ← recR Adenine phosphoribosyltransferase/Recombination protein RecR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,844,9880AT19.3% 285.3 / 25.9 173intergenic (+64/+55)apt/recRAdenine phosphoribosyltransferase/Recombination protein RecR
Reads supporting (aligned to +/- strand):  ref base A (45/91);  new base T (20/14);  total (67/106)
Fisher's exact test for biased strand distribution p-value = 9.59e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

GGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGTGG  >  ACB143/4844854‑4845123
                                                                                                                                            |                                                                                                                                         
gggcgggTCGGTGGCACGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTAt                                                                                                                                          <  1:387440/130‑1 (MQ=255)
    gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAAAACGCGCCTACGCAAGCCGCGGATGttt                                                                                                                                      >  2:61322/1‑136 (MQ=255)
    gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAAACCGCACCTACGAAAGCCGCGGATGtgt                                                                                                                                      >  2:119748/1‑134 (MQ=255)
       tCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAACCCGCGCCAACGCAAGCCGCGGATGtttttt                                                                                                                                   >  4:304887/1‑134 (MQ=39)
        cGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTa                                                                                                                                  <  5:173232/136‑1 (MQ=255)
          gTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAgg                                                                                                                                <  4:138655/136‑1 (MQ=255)
          gTTGCAAGCGGCCGGGGGGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAgg                                                                                                                                <  6:169223/136‑1 (MQ=255)
              aaaGCGGACGGGGTGCCTACATTCTGCCTTACCGAGTTTTCCCTCAGCGCGTATTGATTGGGCATTTGGGGCTGAATTTCAGCACAAT‑‑CGCCGGTAAAACCCCGCCGAAGAAAACCGCGGATTTTTTTTATgcgcc                                                                                                                           >  2:263309/2‑135 (MQ=255)
                   ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTAGCGGGGGTAAACCCCTCGAACAAAAGCCGCGAGTTTTTTTTTGGCggggtgt                                                                                                                       >  4:15870/1‑133 (MQ=255)
                   ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAAACCGCAACGAACGAATCCGCGGATGGTTTTTAGGCGCCGGgtt                                                                                                                      >  1:378834/1‑136 (MQ=255)
                   ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑AGCGGGGTAACCCGCGCCCAACGAAGCCGCGGTTGTGTTTTTGGGGCGGGgtt                                                                                                                      >  7:21234/1‑136 (MQ=255)
                                      tGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAAACCCCACCAACCAAAACCGCGGTTTGTTTTTTGGGGCGGGGTTTACCCCGCAATGGgccccc                                                                                                    >  7:168780/1‑136 (MQ=255)
                                         ctcaCCGAGTTTCCCCTCAGCGAGTATTGCGTGGGCTTTTGGGGCTGCTTTCCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTCCGAACGCCGCGTATGTTTTGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGc                                                                                                <  4:84109/133‑1 (MQ=38)
                                           gACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAAACCGCTGCTACGAAAGCCGCGGATGTTTTGTTTGCGCGGGGTTTCCCGCGCAAGGGCCGCCaacagg                                                                                              >  2:109702/1‑132 (MQ=37)
                                           gACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAAACCCCGCCAACCAAATCCGCCGATGTTTTTTAAGCGCGGGTTTAACCGCCAAATGGCCGCCAACCgg                                                                                              >  3:44475/1‑136 (MQ=255)
                                             ccGAGTTTTCCCTCTCCGAGTATTGAGTGGGCTTGTGGGGCGGCTTGGCAGCCAATT‑‑CGCGGGTAAACCCGCTCCTACGAAACCCGCGTATGTTTTGTAGGCCCGGGTTTACCCGCGAATGGGCCGCCAAGCGGcc                                                                                            <  2:279331/136‑1 (MQ=37)
                                              cGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGGATGTTTTGTAGGCGCCGGGTTACCCGCGAATGGGCCGCCCAGCGGccc                                                                                           >  1:252567/1‑136 (MQ=255)
                                              cGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCCCGCCAACGAAAGCCGCGGATGTTTTGTAGGCGCGGGGTTACCCGCGAATGGGCCGCCCAGCGGccc                                                                                           >  7:95833/1‑136 (MQ=38)
                                               gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAACCCGCTCCGACGCAACCCGCGGATGGTTTTTAGGCGCGGGGTTTCCCGCGAATGGGGCCCCCAGCGGcccc                                                                                          >  5:392701/1‑136 (MQ=37)
                                               gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAACCCGCGCCAAACCAAGCCCCCGATGTTTTTTTTGCGCGGGTTTTCCCCCCAAATGGCCCCCCACCGGgccc                                                                                          >  5:302619/1‑136 (MQ=16)
                                               gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAACCCCCTCCGACGAAAGCCGCGTTTGTTTTTTAAGCGCGGGGTTAACCGCGAAAGGGCCGCCCAGCGGccc                                                                                           >  1:48927/1‑135 (MQ=37)
                                               gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAACCCCCGCCAACGAAAACCGCGGATTTTTTTTATGCGCGGGGTTTACCGCGAATTGGCCGCCCAGCGGgcc                                                                                           >  5:363973/1‑135 (MQ=255)
                                               gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGcccc                                                                                          >  2:309734/1‑136 (MQ=255)
                                               gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAAACCGCTCCAACCAAACCCGCGGATGTTTTTTAGGCGCGGGTTTACCCCCGAATGGGCCGCCCACCCGcccc                                                                                          >  8:188238/1‑136 (MQ=37)
                                               gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCACATT‑‑CGCGGGTTAACCCGCTCCGACGAAAGCCGCGGATGTTTTGTAGGCGCGGGTTTTACCGCGCATGGGGCGCCCAGCCGcccc                                                                                          >  5:91497/1‑136 (MQ=255)
                                                     tCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCCCTCCAACCAAAGCCCCGGATTTTTTTTACGCCCCGGTTTTACCCCCAATGCGGCGCCCAGCCGCCCCAAAGa                                                                                     >  2:220827/1‑135 (MQ=19)
                                                        cTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTTGGCGCGGGTTTACCCGCGAAAGGGCCGCCCAGCGGCCCCAATGAAt                                                                                   >  5:303689/1‑134 (MQ=255)
                                                             gtaGTTTTGAGTGGGCTTTGGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAACCCCGCTCCTACGAACGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCg                                                                           <  6:264861/134‑1 (MQ=38)
                                                             cGATTATTGCGGGGGCTTTTGGGGGTGCTTTGCAGCCCATT‑‑CGCGGGTAACCCCGAGCCTCCGAACGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCg                                                                           <  8:253223/136‑1 (MQ=37)
                                                             cGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCCCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCg                                                                           <  7:299185/136‑1 (MQ=255)
                                                              gAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAAACCGCGGCTAAGAAAGCCGCGTATTTTTTTTATGCCCGGGGTTTCCCGCGGATTGGCCCCCAAGCGGCCCCCATGAATCAGAGCg                                                                           >  8:80066/1‑135 (MQ=255)
                                                              gAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAAACCCCTGCGTCGCAAGCCGCGGTTGTTTTGTAGGCGCGGGGTTTCCCGCGAATGGGCCCCCCACCGGCCCCAATGAATAAGcgcg                                                                           >  7:35531/1‑135 (MQ=255)
                                                              gAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGGATTTTTTGTTGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATAAGAGCg                                                                           >  5:183453/1‑135 (MQ=255)
                                                                  aTTGAGTGCGGTTTTGGGGCGTCTTCCCACCAATTGCCCGGTAAAACCCGCTCAAACTAAAGCGGCTTTTGTTTTGTCGCCGGGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATg                                                                      <  4:395567/136‑1 (MQ=255)
                                                                       gggggCTTTTGCGCCTGCATTGAAGACACTT‑‑CGCTGCTAACCCCCCTCCTACCAAACCCGCTTAGGTTTTGTAGCGGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCg                                                                 <  6:104991/134‑1 (MQ=19)
                                                                        tGGGGTTTTGGGGCGGCTTTGCTGCCCATT‑‑GGGGGGTAAACCCGCGCCTACGAACGCGGCGTATGTTTTGATGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCg                                                                 <  5:181469/135‑1 (MQ=37)
                                                                        tGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCCGGGTAAACCCCGCCATCGCAAGCCCGGGTTTGTTTTTTGGCGCCGGGTTTGCCCCCCAAGGGGCCCCCACCGGGCCCAATTAATACGCGCGCAATTGGCCgg                                                                >  8:156446/1‑136 (MQ=255)
                                                                        tGGGCTTTTGGGGCTGCTTTGCAGCCCATG‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCg                                                                 <  3:50639/135‑1 (MQ=255)
                                                                        tGGGCTTTTGGGCCTGGTTGGCCGCCATTG‑‑CGCGGGTAACCCCGCTCATCCCAACCCCGCTTATGTTTTGTCGGCGCGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCgg                                                                <  8:265870/136‑1 (MQ=255)
                                                                        gggggTTTGGGGGCTGGTTTCCCGCCCTTG‑‑CGCGGGTAACCCCGCTCCTACCAACGCCGCTTATTTTTTGTAGGGGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCg                                                                 <  7:233511/134‑1 (MQ=25)
                                                                        ggggCTTTGGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAACGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCg                                                                 <  2:263323/134‑1 (MQ=255)
                                                                          ggCTTTTGGGGCTGCTTTCCAGCCAATT‑‑CGCGGGAAACCCCGCTCCTACCAACCCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGgc                                                               <  4:340065/135‑1 (MQ=38)
                                                                             ttttGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCGCCTACGAAAGCCGCGGATGTTTTTTAGGCGCGGGGTTACCCGCGAATGGGCCGCCCAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGccc                                                           >  7:105491/1‑136 (MQ=38)
                                                                             ttttGGGGCTGCTTTCCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACCAAACCCGCGTTTGTTTTGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGccc                                                           <  6:174153/136‑1 (MQ=37)
                                                                              tttGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAACCCGCGGCTTCGAAAGCCGCGGTTGTTTTGTAGGCGCGGGGTTTCCCGCGACTGGGGCGGCCAGCGGGCCCCAAGGTTAAGTGCGGAATGGGGCGGCGcc                                                            >  7:261184/1‑134 (MQ=16)
                                                                              tttGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTTAAACCGCGCCGTCGAAACCCCCGGTTTTTTTGTTGGCGCGGGGTTTCCCGCGCATGGGGCGGCAAGCGGGCCCAATGGATAAGCGCGGAATTGGCCGGGGcccc                                                          >  6:14700/1‑135 (MQ=255)
                                                                               ttGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGTAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGGATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCt                                                          <  7:7712/135‑1 (MQ=255)
                                                                               ttGGGGCTGCTTGGCCGCCCATT‑‑CGCGGTGAACCCCGGGCCTACGAAAGCGCGGTATGTTTTGTAGGCGCGGTTTTTCCCGGGAATGGGCCGGCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCt                                                          <  8:192966/135‑1 (MQ=255)
                                                                                tGGGGCGGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGAGCCAACGAACGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTg                                                         <  7:372988/135‑1 (MQ=38)
                                                                                   ggCTGCTTTGCAGCCCATT‑‑CGCGGGTTAACCCGCTGCTACGAAAGCCGCGGTTGTTTTGTAGGCGCGGGGTTACCCGCGCATGGGCCGCCCAGCGGCCCCAATGGATCAGAGCGATATGGGCCGGCGCCCGGCa                                                       >  3:304530/1‑134 (MQ=37)
                                                                                   ggCTGCTTTGCAGCCCATT‑‑CGCGGGTTAAACCCCGCCGACGGAAGGCGCGGTTTGTTTTTTTGGGCGGGGTTTACCGCGGATTGGGCCCCCACCCGGCCCACAGAATGAGCGCGGAAATGGGCCGCGccccggaa                                                      >  4:270630/1‑130 (MQ=255)
                                                                                    ggggctTTGCAGCCCATT‑‑CGCGGGAAAACCCGCTCATACGAAAGCCGCGTTTGTTTTGAAGGCGCGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg                                                    <  4:353100/133‑1 (MQ=255)
                                                                                    gctgctTTGCAGCCCTTT‑‑CGCGGGTAACCCCGCTCCTACGAAACCCGCGTATGTTTTGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg                                                    <  6:370130/136‑1 (MQ=255)
                                                                                    gctgctTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg                                                    <  1:309734/136‑1 (MQ=255)
                                                                                    gctgctTTGCAGCCCATT‑‑CGCGGGAAAACCCGCGCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg                                                    <  7:279688/136‑1 (MQ=255)
                                                                                    gctgctTTCCAGCCCTTT‑‑CGCGGGTAACCCCGCTCCTCCGAAAGCCGCTTTTGTTTTGTCGGGGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg                                                    <  8:36434/136‑1 (MQ=38)
                                                                                    gcggctTTGCAGCCCATT‑‑CGCGGCTAACCCCGATCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg                                                    <  1:101953/133‑1 (MQ=255)
                                                                                    gcggctTTGCAGCCAATG‑‑GGCGGGAAAACCGGCTCCTACGAACGCGGCGTATGTTTTGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg                                                    <  5:229131/133‑1 (MQ=38)
                                                                                    gcggatttGCAGCCCATT‑‑CGCGGTAAACCCCGCTCCTCCGAACGCCGCGTATGTTTTGTCGGCGGGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg                                                    <  7:154047/131‑1 (MQ=255)
                                                                                     ctgctTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACTAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAgg                                                   <  1:55620/136‑1 (MQ=255)
                                                                                         tttGCAGCCCATT‑‑CGCGGGAAACCCCGCTCCAACGAACGCCGCGTATGTTTTGTCGGCGCGGGTTTCCCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATg                                               <  8:121688/136‑1 (MQ=255)
                                                                                          ttggcccccATT‑‑GGGGGGTAAACCCGGCTCCACCGAACGCCGCTTTTGTTTTGTAGGGGGGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATg                                               <  3:197687/130‑1 (MQ=255)
                                                                                           tGCAGCCCATT‑‑CGCGGGTAACCCCGCTCCTACTAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATg                                               <  8:232609/134‑1 (MQ=255)
                                                                                           tGCAGCCCATT‑‑CGCGGGTAACCCCGCGCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATg                                               <  1:136977/134‑1 (MQ=255)
                                                                                           tGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATg                                               <  8:128026/134‑1 (MQ=255)
                                                                                           tGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGAAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATg                                               <  7:18316/134‑1 (MQ=255)
                                                                                           tGCAGCCCATT‑‑CGCGGGTAAACCCGCGCCTACGAAAGCCGCGTTTGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATg                                               <  2:8185/134‑1 (MQ=255)
                                                                                           tGCAGCCCATT‑‑CGCGGGAAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATTGGCCGGCGCCCTGCAAAGGCATg                                               <  2:256620/134‑1 (MQ=255)
                                                                                                        tcccgGGTACACCGGCTCCACCGACCGCCGGGTTTGTGTGGTCGGCGCGGGTATCCCGGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTa                                     <  4:102381/131‑1 (MQ=255)
                                                                                                        cgcgGGTAACCCCCCCCCTACTAAAGCCGCGTATGTTTGGTCGGCGCGGGTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTAccg                                  <  3:229514/136‑1 (MQ=255)
                                                                                                             ggTAACCCCGCTCCTAGGAATGCCGCGTATGTTTTGTAGGCGGGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGt                              <  5:365665/136‑1 (MQ=255)
                                                                                                             ggTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGt                              <  2:250873/136‑1 (MQ=255)
                                                                                                               tAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTcc                            <  6:357226/136‑1 (MQ=255)
                                                                                                                aaaCCCGCGCCTGCGAAAGGCGCGGATGTTTTGTAGGCGCGGGGTTTGCCGCGCATGGGGCGCCAAGCGGCCCCAATGAATCAGAGGGAAATGGGCCGGCGCCCTGCAAAGGCAGGGGCCCGGGTGCCGCCCTccc                           >  2:68144/1‑135 (MQ=255)
                                                                                                                            tACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATTAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGc                >  2:65320/1‑136 (MQ=255)
                                                                                                                            tACGAAAGCCGCGTATGTTTTGTAGGAGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCCCCATCTCCAGc                >  3:170879/1‑136 (MQ=255)
                                                                                                                                aaaGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgc            >  6:70173/1‑136 (MQ=255)
                                                                                                                                 aaGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg                                                    <  4:115773/95‑1 (MQ=255)
                                                                                                                                 aaGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgcc           <  1:11176/136‑1 (MQ=255)
                                                                                                                                 aaGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgcc           <  6:196789/136‑1 (MQ=255)
                                                                                                                                  accccGCTTATTTTTTGGCGGCGCGGGTTACCCCGCTAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgcc           <  1:187734/133‑1 (MQ=255)
                                                                                                                                   gCCGCGTATGTTTTGTAGGCGCGGGATTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgccgc         <  3:305955/136‑1 (MQ=255)
                                                                                                                                    cccGCGTCTGTTTGGTAGGCGCGGGTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgccgc         <  4:188644/135‑1 (MQ=255)
                                                                                                                                      cgcgTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCgccgcc        >  3:353566/1‑135 (MQ=255)
                                                                                                                                      cgcgTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGccc       <  1:257085/136‑1 (MQ=255)
                                                                                                                                       gcgTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCgccgcc        >  8:315012/1‑134 (MQ=255)
                                                                                                                                       gcgTATGTTTTGTAGGCGCGGGTTTACCCGAGAATGGGCCGCAAAGCGGCCCCAATGCATCAGAGCGAAAATGGCCGGCGCCCTGAAAAGGCATGGGCCAGGGTACCTCCGGACACAATATCCAGCTAgccgcc        >  3:324131/1‑134 (MQ=25)
                                                                                                                                        cgTATGTTTTGTCGGCGCGGGTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAg     <  4:399455/136‑1 (MQ=255)
                                                                                                                                          gTATTTTTTGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt    <  4:193156/136‑1 (MQ=255)
                                                                                                                                          gTATTTTTTGTAGCCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt    <  3:235289/136‑1 (MQ=255)
                                                                                                                                          gTATGTTTTGTCGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt    <  1:243968/136‑1 (MQ=255)
                                                                                                                                          gTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt    <  6:271097/136‑1 (MQ=255)
                                                                                                                                          gTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt    <  6:179699/136‑1 (MQ=255)
                                                                                                                                          gTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt    <  8:34148/136‑1 (MQ=255)
                                                                                                                                          gTATGTTTTGACGGCGCGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt    <  5:203202/136‑1 (MQ=255)
                                                                                                                                            aTGTTTTGTAGGCGCGGGTTTACCCGCGAATTGGCCCCCAAGCGGCCCCAAAGAATCAGAGCGAAAAGGGCCGGCGCCCTGCAAAGGCAAGGGCCACGGTACCGCCGTCCCCCATATCCAGCTCGCCGCCCAGttg  >  8:286093/1‑134 (MQ=255)
                                                                                                                                            aTGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCt                                                          <  3:133490/80‑1 (MQ=255)
                                                                                                                                            aTGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt    <  5:121764/134‑1 (MQ=255)
                                                                                                                                            aTGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGTgg  >  7:62877/1‑136 (MQ=255)
                                                                                                                                            aTGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCCCCAAGCGGCCCCAATGAAACAGAGCGAAAAGGGCCGGCGCCCTGCAAACGCATGGGCCAGGGGACCGCCGTCCACCATCTCCACCACACCCCCCAGTgg  >  2:383049/1‑136 (MQ=38)
                                                                                                                                            |                                                                                                                                         
GGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGTGG  >  ACB143/4844854‑4845123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: