Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB143 | 4,844,988 | A→T | 19.3% | intergenic (+64/+55) | apt → / ← recR | Adenine phosphoribosyltransferase/Recombination protein RecR |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 4,844,988 | 0 | A | T | 19.3% | 285.3 / 25.9 | 173 | intergenic (+64/+55) | apt/recR | Adenine phosphoribosyltransferase/Recombination protein RecR |
Reads supporting (aligned to +/- strand): ref base A (45/91); new base T (20/14); total (67/106) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 9.59e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 183 total aligned reads displayed.
GGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGTGG > ACB143/4844854‑4845123 | gggcgggTCGGTGGCACGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTAt < 1:387440/130‑1 (MQ=255) gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAAAAC‑GCGCCTACGCAAG‑CCGC‑GGATGttt > 2:61322/1‑136 (MQ=255) gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAAACC‑GCACCTACGAAAG‑CCGC‑GGATGtgt > 2:119748/1‑134 (MQ=255) tCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAACCC‑GCGCCAACGCAAG‑CCGC‑GGATGtttttt > 4:304887/1‑134 (MQ=39) cGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTa < 5:173232/136‑1 (MQ=255) gTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAgg < 4:138655/136‑1 (MQ=255) gTTGCAAGCGGCCGGGGGGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAgg < 6:169223/136‑1 (MQ=255) aaaGCGGACGGGGTGCCTACATTCTGCCTTACCGAGTTTTCCCTCAGCGCGTATTGATTGGGCATTTGGGGCTGAATTTCAGCACAAT‑‑CG‑CCGGTAAAACC‑CCGCCGAAGAAAA‑CCGC‑GGATTTTTTTTATgcg‑cc > 2:263309/2‑135 (MQ=255) ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTAGCG‑GGGGTAAACCC‑‑CTCGAACAAAAG‑CCGC‑GAGTTTTTTTTTGGCg‑gggtgt > 4:15870/1‑133 (MQ=255) ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAAACC‑GCAACGAACGAAT‑CCGC‑GGATGGTTTTTAGGCG‑CCGGgtt > 1:378834/1‑136 (MQ=255) ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑AG‑CGGGGTAACCC‑GCGCCCAACGAAG‑CCGC‑GGTTGTGTTTTTGGGG‑CGGGgtt > 7:21234/1‑136 (MQ=255) tGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAAACC‑CCACCAACCAAAA‑CCGCGGTTTGTTTTTTGGGGC‑GGGGTTTACCCCGCAATGGgccccc > 7:168780/1‑136 (MQ=255) ctcaCCGAGTTTCCCCTCAGCGAGTATTGCGTGGGCTTTTGGGGCTGCTTTCCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTCCGAACG‑CCGC‑GTATGTTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGc < 4:84109/133‑1 (MQ=38) gACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAAACC‑GCTGCTACGAAAG‑CCGC‑GGATGTTTTGTTTGCG‑CGGGGTTTCCCGCGCAAGGGCCGCCaacagg > 2:109702/1‑132 (MQ=37) gACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAAACC‑CCGCCAACCAAAT‑CCGC‑CGATGTTTTTTAAGCG‑CGGGTTTAACCGCCAAATGGCCGCCAACCgg > 3:44475/1‑136 (MQ=255) ccGAGTTTTCCCTCTCCGAGTATTGAGTGGGCTTGTGGGGCGGCTTGGCAGCCAATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAC‑CCGC‑GTATGTTTTGTAGGCC‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGcc < 2:279331/136‑1 (MQ=37) cGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GGATGTTTTGTAGGCG‑CCGGGTTACCCGCGAATGGGCCGCCCAGCGGccc > 1:252567/1‑136 (MQ=255) cGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑CCGCCAACGAAAG‑CCGC‑GGATGTTTTGTAGGCG‑CGGGGTTACCCGCGAATGGGCCGCCCAGCGGccc > 7:95833/1‑136 (MQ=38) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAACCC‑GCTCCGACGCAAC‑CCGC‑GGATGGTTTTTAGGCG‑CGGGGTTTCCCGCGAATGGGGCCCCCAGCGGcccc > 5:392701/1‑136 (MQ=37) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAACCC‑GCGCCAAACCAAG‑CCCC‑CGATGTTTTTTTTGCG‑CGGGTTTTCCCCCCAAATGGCCCCCCACCGGgccc > 5:302619/1‑136 (MQ=16) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAACCC‑CCTCCGACGAAAG‑CCGC‑GTTTGTTTTTTAAGCG‑CGGGGTTAACCGCGAAAGGGCCGCCCAGCGGccc > 1:48927/1‑135 (MQ=37) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAACCC‑CCGCCAACGAAAA‑CCGC‑GGATTTTTTTTATGCG‑CGGGGTTTACCGCGAATTGGCCGCCCAGCGGgcc > 5:363973/1‑135 (MQ=255) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGcccc > 2:309734/1‑136 (MQ=255) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAAACC‑GCTCCAACCAAAC‑CCGC‑GGATGTTTTTTAGGCG‑CGGGTTTACCCCCGAATGGGCCGCCCACCCGcccc > 8:188238/1‑136 (MQ=37) gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCACATT‑‑CG‑CGGGTTAACCC‑GCTCCGACGAAAG‑CCGC‑GGATGTTTTGTAGGCG‑CGGGTTTTACCGCGCATGGGGCGCCCAGCCGcccc > 5:91497/1‑136 (MQ=255) tCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑CCTCCAACCAAAG‑CCCC‑GGATTTTTTTTACGCC‑CCGGTTTTACCCCCAATGCGGCGCCCAGCCGCCCCAAAGa > 2:220827/1‑135 (MQ=19) cTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTTGGCG‑CGGGTTTACCCGCGAAAGGGCCGCCCAGCGGCCCCAATGAAt > 5:303689/1‑134 (MQ=255) gtaGTTTTGAGTGGGCTTTGGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAACCCC‑GCTCCTACGAACG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑g < 6:264861/134‑1 (MQ=38) cGATTATTGCGGGGGCTTTTGGGGGTGCTTTGCAGCCCATT‑‑CG‑CGGGTAACCCC‑GAGCCTCCGAACG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑g < 8:253223/136‑1 (MQ=37) cGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCCCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑g < 7:299185/136‑1 (MQ=255) gAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAAACC‑GCGGCTAAGAAAG‑CCGC‑GTATTTTTTTTATGCC‑CGGGGTTTCCCGCGGATTGGCCCCCAAGCGGCCCCCATGAATCAGAGC‑g > 8:80066/1‑135 (MQ=255) gAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAAACC‑CCTGCGTCGCAAG‑CCGC‑GGTTGTTTTGTAGGCG‑CGGGGTTTCCCGCGAATGGGCCCCCCACCGGCCCCAATGAATAAGcgc‑g > 7:35531/1‑135 (MQ=255) gAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GGATTTTTTGTTGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATAAGAGC‑g > 5:183453/1‑135 (MQ=255) aTTGAGTGCGGTTTTGGGGCGTCTTCCCA‑CCAATTG‑CC‑CGGTAAAACCC‑GCTCAAACTAAAG‑CGGC‑TTTTGTTTTGTCGCCG‑GGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATg < 4:395567/136‑1 (MQ=255) gggggCTTTTGCGCCTGCATTGAAGACACTT‑‑CG‑CTGCTAACCCC‑CCTCCTACCAAAC‑CCGC‑TTAGGTTTTGTAGCGG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg < 6:104991/134‑1 (MQ=19) tGGGGTTTTGGGGCGGCTTTGCTGCCCATT‑‑GG‑GGGGTAAACCC‑GCGCCTACGAACG‑CGGC‑GTATGTTTTGATGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg < 5:181469/135‑1 (MQ=37) tGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CGCCGGGTAAACCC‑‑CGCCATCGCAAGCCCGG‑GTTTGTTTT‑TTGGCGCCGGGTTTGCCCCCCAAGGGGCC‑CCCACCGGGCCCAATTAATACGCGC‑GCAATTGGCCgg > 8:156446/1‑136 (MQ=255) tGGGCTTTTGGGGCTGCTTTGCAGCCCATG‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg < 3:50639/135‑1 (MQ=255) tGGGCTTTTGGGCCTGGTTGGCCGCCATTG‑‑CG‑CGGGTAACCCC‑GCTCATCCCAACC‑CCGC‑TTATGTTTTGTCGGCG‑CGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCgg < 8:265870/136‑1 (MQ=255) gggggTTTGGGGGCTGGTTTCCCGCCCTTG‑‑CG‑CGGGTAACCCC‑GCTCCTACCAACG‑CCGC‑TTATTTTTTGTAGGGG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg < 7:233511/134‑1 (MQ=25) ggggCTTTGGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAACG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg < 2:263323/134‑1 (MQ=255) ggCTTTTGGGGCTGCTTTCCAGCCAATT‑‑CG‑CGGGAAACCCC‑GCTCCTACCAACC‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGgc < 4:340065/135‑1 (MQ=38) ttttGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCGCCTACGAAAG‑CCGC‑GGATGTTTTTTAGGCG‑CGGGGTTACCCGCGAATGGGCCGCCCAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGccc > 7:105491/1‑136 (MQ=38) ttttGGGGCTGCTTTCCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACCAAAC‑CCGC‑GTTTGTTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGccc < 6:174153/136‑1 (MQ=37) tttGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAACCC‑GCGGCTTCGAAAG‑CCGC‑GGTTGTTTTGTAGGCG‑CGGGGTTTCCCGCGACTGGGGCGGCCAGCGGGCCCCAAGGTTAAGTGC‑GGAATGGGGCGGCGcc > 7:261184/1‑134 (MQ=16) tttGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAAACC‑GCGCCGTCGAAAC‑CCCC‑GGTTTTTTTGTTGGCG‑CGGGGTTTCCCGCGCATGGGGCGGCAAGCGGGCCCAATGGATAAGCGC‑GGAATTGGCCGGGGcccc > 6:14700/1‑135 (MQ=255) ttGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGTAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGGATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCt < 7:7712/135‑1 (MQ=255) ttGGGGCTGCTTGGCCGCCCATT‑‑CG‑CGGTGAACCCC‑GGGCCTACGAAAG‑CGCG‑GTATGTTTTGTAGGCG‑CGGTTTTTCCCGGGAATGGGCCGGCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCt < 8:192966/135‑1 (MQ=255) tGGGGCGGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GAGCCAACGAACG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTg < 7:372988/135‑1 (MQ=38) ggCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAACCC‑GCTGCTACGAAAG‑CCGC‑GGTTGTTTTGTAGGCG‑CGGGGTTACCCGCGCATGGGCCGCCCAGCGGCCCCAATGGATCAGAGC‑GATATGGGCCGGCGCCCGGCa > 3:304530/1‑134 (MQ=37) ggCTGCTTTGCAGCCCATT‑‑CG‑CGGGTTAAACC‑CCGCCGACGGAAG‑GCGCGGTTTGTTTTTTTGGGC‑GGGGTTTACCGCGGATTGGGCCCCCACCCGG‑CCCACAGAATGAGCGCGGAAATGGGCC‑GCGccccggaa > 4:270630/1‑130 (MQ=255) ggggctTTGCAGCCCATT‑‑CG‑CGGGAAAACCC‑GCTCATACGAAAG‑CCGC‑GTTTGTTTTGAAGGCG‑CGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg < 4:353100/133‑1 (MQ=255) gctgctTTGCAGCCCTTT‑‑CG‑CGGGTAACCCC‑GCTCCTACGAAAC‑CCGC‑GTATGTTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg < 6:370130/136‑1 (MQ=255) gctgctTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg < 1:309734/136‑1 (MQ=255) gctgctTTGCAGCCCATT‑‑CG‑CGGGAAAACCC‑GCGCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg < 7:279688/136‑1 (MQ=255) gctgctTTCCAGCCCTTT‑‑CG‑CGGGTAACCCC‑GCTCCTCCGAAAG‑CCGC‑TTTTGTTTTGTCGGGG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg < 8:36434/136‑1 (MQ=38) gcggctTTGCAGCCCATT‑‑CG‑CGGCTAACCCC‑GATCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg < 1:101953/133‑1 (MQ=255) gcggctTTGCAGCCAATG‑‑GG‑CGGGAAAACCG‑GCTCCTACGAACG‑CGGC‑GTATGTTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg < 5:229131/133‑1 (MQ=38) gcggatttGCAGCCCATT‑‑CG‑CGGTAAACCCC‑GCTCCTCCGAACG‑CCGC‑GTATGTTTTGTCGGCG‑GGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg < 7:154047/131‑1 (MQ=255) ctgctTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACTAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAgg < 1:55620/136‑1 (MQ=255) tttGCAGCCCATT‑‑CG‑CGGGAAACCCC‑GCTCCAACGAACG‑CCGC‑GTATGTTTTGTCGGCG‑CGGGTTTCCCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 8:121688/136‑1 (MQ=255) ttggcccccATT‑‑GG‑GGGGTAAACCCGGCTCCACCGAACG‑CCGC‑TTTTGTTTTGTAGGGG‑GGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 3:197687/130‑1 (MQ=255) tGCAGCCCATT‑‑CG‑CGGGTAACCCC‑GCTCCTACTAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 8:232609/134‑1 (MQ=255) tGCAGCCCATT‑‑CG‑CGGGTAACCCC‑GCGCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 1:136977/134‑1 (MQ=255) tGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 8:128026/134‑1 (MQ=255) tGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGAAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 7:18316/134‑1 (MQ=255) tGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCGCCTACGAAAG‑CCGC‑GTTTGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg < 2:8185/134‑1 (MQ=255) tGCAGCCCATT‑‑CG‑CGGGAAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATTGGCCGGCGCCCTGCAAAGGCATg < 2:256620/134‑1 (MQ=255) tcccgGGTACACCG‑GCTCCACCGACCG‑CCGG‑GTTTGTGTGGTCGGCG‑CGGGTATCCCGGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTa < 4:102381/131‑1 (MQ=255) cg‑cgGGTAACCCC‑CCCCCTACTAAAG‑CCGC‑GTATGTTTGGTCGGCG‑CGGGTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTAccg < 3:229514/136‑1 (MQ=255) ggTAACCCC‑GCTCCTAGGAATG‑CCGC‑GTATGTTTTGTAGGCG‑GGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGt < 5:365665/136‑1 (MQ=255) ggTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGt < 2:250873/136‑1 (MQ=255) tAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTcc < 6:357226/136‑1 (MQ=255) aaaCCC‑GCGCCTGCGAAAG‑GCGC‑GGATGTTTTGTAGGCG‑CGGGGTTTGCCGCGCATGGGGCGCCAAGCGGCCCCAATGAATCAGAGG‑GAAATGGGCCGGCGCCCTGCAAAGGCAGGGGCCCGGGTGCCGCCCTccc > 2:68144/1‑135 (MQ=255) tACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATTAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGc > 2:65320/1‑136 (MQ=255) tACGAAAG‑CCGC‑GTATGTTTTGTAGGAG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCCCCATCTCCAGc > 3:170879/1‑136 (MQ=255) aaaG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgc > 6:70173/1‑136 (MQ=255) aaG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg < 4:115773/95‑1 (MQ=255) aaG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgcc < 1:11176/136‑1 (MQ=255) aaG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgcc < 6:196789/136‑1 (MQ=255) accccGC‑TTATTTTTTGGCGGCG‑CGGGTTACCCCGCTAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgcc < 1:187734/133‑1 (MQ=255) g‑CCGC‑GTATGTTTTGTAGGCG‑CGGGATTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgccgc < 3:305955/136‑1 (MQ=255) cccGC‑GTCTGTTTGGTAGGCG‑CGGGTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTcgccgc < 4:188644/135‑1 (MQ=255) cgc‑gTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCgccgcc > 3:353566/1‑135 (MQ=255) cgc‑gTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGccc < 1:257085/136‑1 (MQ=255) gc‑gTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCgccgcc > 8:315012/1‑134 (MQ=255) gc‑gTATGTTTTGTAGGCG‑CGGGTTTACCCGAGAATGGGCCGCAAAGCGGCCCCAATGCATCAGAGC‑GAAAATGGCCGGCGCCCTGAAAAGGCATGGGCCAGGGTACCTCCGGACACAATATCCAGCTAgccgcc > 3:324131/1‑134 (MQ=25) c‑gTATGTTTTGTCGGCG‑CGGGTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAg < 4:399455/136‑1 (MQ=255) gTATTTTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt < 4:193156/136‑1 (MQ=255) gTATTTTTTGTAGCCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt < 3:235289/136‑1 (MQ=255) gTATGTTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt < 1:243968/136‑1 (MQ=255) gTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt < 6:271097/136‑1 (MQ=255) gTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt < 6:179699/136‑1 (MQ=255) gTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt < 8:34148/136‑1 (MQ=255) gTATGTTTTGACGGCG‑CGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt < 5:203202/136‑1 (MQ=255) aTGTTTTGTAGGCG‑CGGGTTTACCCGCGAATTGGCCCCCAAGCGGCCCCAAAGAATCAGAGC‑GAAAAGGGCCGGCGCCCTGCAAAGGCAAGGGCCACGGTACCGCCGTCCCCCATATCCAGCTCGCCGCCCAGttg > 8:286093/1‑134 (MQ=255) aTGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCt < 3:133490/80‑1 (MQ=255) aTGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGt < 5:121764/134‑1 (MQ=255) aTGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGTgg > 7:62877/1‑136 (MQ=255) aTGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCCCCAAGCGGCCCCAATGAAACAGAGC‑GAAAAGGGCCGGCGCCCTGCAAACGCATGGGCCAGGGGACCGCCGTCCACCATCTCCACCACACCCCCCAGTgg > 2:383049/1‑136 (MQ=38) | GGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑‑CG‑CGGGTAAACCC‑GCTCCTACGAAAG‑CCGC‑GTATGTTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCATCTCCAGCTCGCCGCCCAGTGG > ACB143/4844854‑4845123 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |