Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,926,694 G→A 5.9% I357I (ATC→ATT)  srp54 ← Signal recognition particle 54 kDa protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,926,6940GA 5.9% 230.9 / 3.2 68I357I (ATC→ATT) srp54Signal recognition particle 54 kDa protein
Reads supporting (aligned to +/- strand):  ref base G (31/33);  new base A (2/2);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

CTGGTGGCCCCGCGGCAGGTGCAGGTCGTCAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAT  >  ACB143/4926562‑4926825
                                                                                                                                    |                                                                                                                                   
cTGGTGGCCCCGCGGCAGGTGCAGGTCGTCAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATg                                                                                                                                  <  1:204005/136‑1 (MQ=255)
           gcgGCAGGTGCAGGTCGTCAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCggccag                                                                                                                       >  6:123266/1‑136 (MQ=255)
             ggCAGGTGCAGGTCGTCAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCggccaggc                                                                                                                     >  4:192639/1‑136 (MQ=255)
                    gcaggtCGTCAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAgcg                                                                                                              >  7:81064/1‑136 (MQ=255)
                        gtcgtcAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTg                                                                                                          <  7:279150/136‑1 (MQ=255)
                        gtcgtcAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTg                                                                                                          <  7:42938/136‑1 (MQ=255)
                          cgtcAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACCGCGCTTg                                                                                                          >  8:349876/1‑134 (MQ=255)
                          cgtcAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTa                                                                                                        <  4:151434/136‑1 (MQ=255)
                                     cGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAgg                                                                                             <  2:244411/136‑1 (MQ=255)
                                     cGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGAATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAgg                                                                                             >  3:274164/1‑136 (MQ=255)
                                      gTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGTGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAggc                                                                                            >  5:105857/1‑136 (MQ=255)
                                          gCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGTTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGgcggcg                                                                                        >  5:34416/1‑136 (MQ=255)
                                                ggTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGc                                                                                  <  5:331136/136‑1 (MQ=255)
                                                 gTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGATTGTAGCTGTGGTAGGCGGCGGTCAGCa                                                                                 >  4:93871/1‑136 (MQ=255)
                                                     gATAGGACACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTg                                                                             <  2:160105/136‑1 (MQ=255)
                                                       tAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCt                                                                           <  5:378267/136‑1 (MQ=255)
                                                          gCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTct                                                                        <  4:264500/136‑1 (MQ=255)
                                                          gCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTct                                                                        <  8:146234/136‑1 (MQ=255)
                                                           ccACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTctg                                                                       >  3:159350/1‑136 (MQ=255)
                                                             aCTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTctggc                                                                     >  8:213857/1‑136 (MQ=255)
                                                             aCTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTctggc                                                                     >  4:187024/1‑136 (MQ=255)
                                                             aCTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTctggc                                                                     >  6:213676/1‑136 (MQ=255)
                                                                 gCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTctggctg                                                                   <  6:355297/134‑1 (MQ=255)
                                                                 gCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTctggctg                                                                   <  6:355300/134‑1 (MQ=255)
                                                                  cAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCtggtg                                                                >  4:233346/1‑136 (MQ=255)
                                                                   aGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCtggtgg                                                               >  3:104881/1‑136 (MQ=255)
                                                                   aGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGAATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCtggtgg                                                               >  5:381283/1‑136 (MQ=255)
                                                                    gTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCtggtggt                                                              <  3:163777/136‑1 (MQ=255)
                                                                        aTGACTGATGGCAAGGCTCAACACCTCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGc                                                           <  8:132762/135‑1 (MQ=255)
                                                                        aTGACTGATGGCAAGGCTCAACACATAGCCAAGGCTTGCCGATTCATCGAGTTAGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGAAGGCGGCGGTCAGCACCTGCTACTGGCTGGTGGAGGc                                                           >  8:237869/1‑135 (MQ=255)
                                                                         tGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCa                                                         <  5:123266/136‑1 (MQ=255)
                                                                         tGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTAGTGGc                                                           >  4:59555/1‑134 (MQ=255)
                                                                            cTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCa                                                      >  6:183385/1‑136 (MQ=255)
                                                                               aTGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGAATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCAcc                                                    <  4:114210/135‑1 (MQ=255)
                                                                                  gCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCtgt                                                                                                   >  1:273432/1‑85 (MQ=255)
                                                                                  gCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCtgt                                                                                                   <  2:273432/85‑1 (MQ=255)
                                                                                  gCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAgg                                                 >  2:39223/1‑135 (MQ=255)
                                                                                  gCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAgg                                                 >  6:291606/1‑135 (MQ=255)
                                                                                  gCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGt                                                >  7:258584/1‑136 (MQ=255)
                                                                                           aaCACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTcg                                       <  3:225565/136‑1 (MQ=255)
                                                                                            acacATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCCGCACCAGGTAGTTCTTcgc                                      >  2:62049/1‑136 (MQ=255)
                                                                                              acaTCGCCAAGGCTTGCCGTTTCTTCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCgg                                    >  2:335940/1‑136 (MQ=255)
                                                                                                    ccAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGc                              >  5:61628/1‑136 (MQ=255)
                                                                                                     cAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGcc                             >  3:173962/1‑136 (MQ=255)
                                                                                                     cAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGcc                             <  8:293416/136‑1 (MQ=255)
                                                                                                     cAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGcc                             >  6:236911/1‑136 (MQ=255)
                                                                                                     cAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGc                              >  3:267822/1‑135 (MQ=255)
                                                                                                     cAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGc                              >  7:348063/1‑135 (MQ=255)
                                                                                                     cAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGc                              >  3:60856/1‑135 (MQ=255)
                                                                                                        ggCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACg                          >  2:393773/1‑136 (MQ=255)
                                                                                                                gTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCtggtggtgg                                                            <  3:171109/94‑1 (MQ=255)
                                                                                                                gTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCtggtggtgg                                                            >  4:171109/1‑94 (MQ=255)
                                                                                                                      tCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGttt            >  3:372054/1‑136 (MQ=255)
                                                                                                                      tCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGttt            <  4:104556/136‑1 (MQ=255)
                                                                                                                      tCGAGTTTGGTCAGAATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGttt            <  4:77593/136‑1 (MQ=255)
                                                                                                                        gAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTcc          <  6:48820/136‑1 (MQ=255)
                                                                                                                           tttGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGt       <  2:201982/136‑1 (MQ=255)
                                                                                                                           tttGGTCAGGATGCAGCCGGCCAGGCCACAGCGCGTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGt       <  4:205731/136‑1 (MQ=255)
                                                                                                                                tCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAt  <  7:213857/136‑1 (MQ=255)
                                                                                                                                tCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAt  <  4:78631/136‑1 (MQ=255)
                                                                                                                                tCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAt  <  6:31604/136‑1 (MQ=255)
                                                                                                                                tCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAt  <  6:16420/136‑1 (MQ=255)
                                                                                                                                tCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAt  <  8:27669/136‑1 (MQ=255)
                                                                                                                                tCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAt  <  8:365896/136‑1 (MQ=255)
                                                                                                                                tCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAt  <  8:45798/136‑1 (MQ=255)
                                                                                                                                tCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAAAGCCACGCCCGGCCAAGGTTTCCAGTTGCAt  <  3:204253/136‑1 (MQ=255)
                                                                                                                                tCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTGCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAt  <  6:163702/136‑1 (MQ=255)
                                                                                                                                gcAGGAGGCAGCCGGCGAGGCCACAGCGCTTGTCGCTGGGGGAGGCGGCGGTCAGCACCTGCTGCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCACGGTTTCCAGTTGCAt  <  7:237869/135‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                   
CTGGTGGCCCCGCGGCAGGTGCAGGTCGTCAGGAATGCGTGGGCCATCGGTAAGATAGGCCACTGGCAGTTCATGACTGATGGCAAGGCTCAACACATCGCCAAGGCTTGCCGTTTCATCGAGTTTGGTCAGGATGCAGCCGGCCAGGCCACAGCGCTTGTAGCTGTGGTAGGCGGCGGTCAGCACCTGCTTCTGGCTGGTGGTGGCCAGCACCAGGTAGTTCTTCGCGGCAATGCCACGCCCGGCCAAGGTTTCCAGTTGCAT  >  ACB143/4926562‑4926825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑