Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,927,190 T→G 32.2% Q192P (CAG→CCG)  srp54 ← Signal recognition particle 54 kDa protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,927,1900TG32.2% 21.0 / 7.0 44Q192P (CAG→CCG) srp54Signal recognition particle 54 kDa protein
Reads supporting (aligned to +/- strand):  ref base T (22/6);  new base G (3/12);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 3.21e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

TCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCA  >  ACB143/4927077‑4927307
                                                                                                                 |                                                                                                                     
tCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGGTCTGCGATCGcggc                                                                                                 >  7:105617/1‑136 (MQ=255)
tCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTGTTCGATCTcggc                                                                                                 >  7:288883/1‑136 (MQ=255)
tCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGCTCTcggc                                                                                                 >  6:200041/1‑136 (MQ=255)
 cTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCGCGATTTCAGGCACTTCGATCATCCGTGCAAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGCtcaggggg                                                                                                >  4:275942/1‑130 (MQ=255)
 cTTGCCCATGCCCGCCGGGCCGACCATGGCGAGCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTATTCGATCTcggcg                                                                                                <  5:236911/136‑1 (MQ=255)
 cTTCCCCAGGCCCGCCGGGCCGACCAGGGCGACCACCCCACCCTGTTCGAGCGGCTCGATTTCAGGCACTCCGTTCATCCGTGCCATGGGCGCCAGCCGCATGGCCCAGGCGGGGGGCGGCTCTTCGATCTcggcg                                                                                                <  2:321614/136‑1 (MQ=255)
     ccgaTGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCACGCCTGGCGCGGCGCTTCGATCTcggcggg                                                                                              >  7:369474/4‑133 (MQ=255)
       cATGCCCGCCGGGCCGACCATGGCGATCCCCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCTCTTGGTTCGaggcggggagg                                                                                          >  3:350160/1‑131 (MQ=255)
       cATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCTGCGCTCTcggcggggagg                                                                                          >  1:354602/1‑131 (MQ=255)
             cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCGCGTCGGGCGCGGCGGGCAGGGCcagc                                                                                    >  5:277565/1‑136 (MQ=255)
             cgccgGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCACGCCTGGCGCGGCTCGTGGAGCTCGGCGGGCAGGTCcagc                                                                                    >  4:17898/1‑136 (MQ=255)
                 gggCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCACGCAGGGCCCGGGGCCAAGCGCTCGGCGGTACGGTCCACCAGCt                                                                                >  1:307113/1‑136 (MQ=255)
                       aCCATGGCGAACACCCCACCCTCTTCGACCGGCGCGATTTCCGCCACTTCCACCATCCGTCCCAAGTGAGCCCCCAGCATGCCCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                          <  2:397605/136‑1 (MQ=255)
                       aCCATGCCGATCCCCCCCCCCTCTACCACCGCCTCGATTACAGGCACTTCGCTCAACCGTGCCACGGCCCCCAGCACCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                          <  1:17753/136‑1 (MQ=255)
                       aCCATGCCCACCACCCCCCCCTCTTCGCGCGGCGCCATTTCAGGCCCTGCGCTCAGCCGCGCCACGGGCGCCCGCCGCCGGCGCCCGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                          <  4:173962/136‑1 (MQ=255)
                       aCCAGGGCGAGCACCCCACCCTCTTCGAGCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCggg                                                                          <  8:91162/136‑1 (MQ=255)
                                   cccccacccTCTCCGAGCGGCTCGATTTCCGCCACTCCGATCCCCCGGCCCAAGCCCGCCAGCCGCACGCGCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCgg                                                              <  7:26017/135‑1 (MQ=255)
                                        acccTCTTCGAGCGGCGCGATTACCGCCACTTCGACCCCCCGTCCCAAGGGCGCCCGCAGCATGCGCCCGCCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAgg                                                         <  2:342403/136‑1 (MQ=255)
                                            tctTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTAGATATCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGcccc                                                     >  1:389154/1‑134 (MQ=255)
                                                 gATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCTCTTAGCTCTAGGCGGTCCTGTCCAGCAGCTCGCGGGCGATCGGCCCCGACCGGCCGATgcg                                                 >  5:110059/1‑135 (MQ=255)
                                                 gATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCCGCCAGGCCCATgcgc                                                >  7:111286/1‑136 (MQ=255)
                                                 gATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCCCCAGGCCTGGCGCGGCGCTTCGCTCTCGGCGGTCAGGGCCAGCAGCTCGCGGGCGATCGGGCCGGCCACGCCGATgcgc                                                >  5:106177/1‑136 (MQ=255)
                                                  aTCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCGCTTCGATCAAGGCGGTCAGGTCCACCAGCTACCCGGCGATAGGGCCGGACCCGCCCAAgcgcg                                               >  2:177840/1‑135 (MQ=255)
                                                  aTCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCGGGCGCGGCGCTTAGATATCGGCGGGGAGGTCCAGCACCTCGCGGGCGATCGGCCCCGCCACGCCCCTgcg                                                 >  4:154928/1‑134 (MQ=255)
                                                     gggtCGTTTTCTGCCAATTAGATCATCCTTCCCTAGTTCGCCCCCAGTAGGCCGCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCa                                            <  4:354733/133‑1 (MQ=255)
                                                      gCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGCACAAGCCGATGCgcagcac                                           >  1:326543/1‑135 (MQ=255)
                                                         cGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCAGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGCTCGGCCCGGGCCGGCCCCTGCGCTGcccgcg                                        >  5:142992/1‑131 (MQ=255)
                                                         cGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATTCGCTGCAggcg                                        >  7:1455/1‑136 (MQ=255)
                                                            tttCCGGCACTTCGATCATCCGTGCCATGTGACCATGCCTCATCGCCCTGGCCGGCCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAggcggc                                      <  3:37891/135‑1 (MQ=255)
                                                                agccACTGCGCTCAGCCGGGCCAAGTTCGCCCGCAGCATGCGCCTGGCCTGGGGCGGCGCGTCGATCTCGGCGGTCAGGTCCAGCATCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCGGCAGGCGGCGCCaa                                 <  2:362447/133‑1 (MQ=255)
                                                                     gttCTATCATCGGTGCCATGGGTGCCAGCCGCAGGCGCCAGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGtt                            <  4:242455/135‑1 (MQ=255)
                                                                     cTTCGACCTGCCGTGCCATGTGCGCCAGCCCCTGGCGCCAGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGtt                            <  2:326543/136‑1 (MQ=255)
                                                                     cTTCCATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCGGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGtt                            <  4:237803/136‑1 (MQ=255)
                                                                         gATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCTCTTCGATCTCGGCGGTGAGGTCCAGCAGCTCGCGGGCGCTAGGCCCGGCCAGGCCGCTGCGCTGCAGGCGGCGCCAAAGGTTGGcc                        >  4:79415/1‑136 (MQ=255)
                                                                         gATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGCTCTCGGCGGGCAGGTCCAGCCGCTCGCGGGCGATCGGCCCGGCCCGGCCGCTGCGCTGCCGGCGGCGCCAAAGGTTGGcc                        >  2:55267/1‑136 (MQ=255)
                                                                         gATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGAGCTCGGCGGGCAGGTGCCGCAGCTCGCGGGCGCTCGGCCCGGGCCGGCCGCTGCGCTGCCGGCGGCGCCCAACGTTGGcc                        >  5:355807/1‑136 (MQ=255)
                                                                             atcCGGGCCAAGGGCGCCAGCCGCATGCGCCAGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCctgct                    <  8:91048/136‑1 (MQ=255)
                                                                                  tcccAAGTGCGCCAGCCGCAGGCGCCTGGCCGGCCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGc               <  2:45195/134‑1 (MQ=255)
                                                                                   gCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCCGCTCGCGGGCGATCGGCCCGGCCAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCt              >  7:337592/1‑136 (MQ=255)
                                                                                   gCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGAACGGCCCCGCCAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCt              >  7:207785/1‑136 (MQ=255)
                                                                                     cAAGTGAGCCAGCAGCATGCGCCCGGCCTGGCGCGGCTCTTCGATCTAGGCGGTCAGGTCCAGCAGCTCGCGGGCGATAGGCCCGGCCCGGCCGCTGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCttt            >  4:72248/1‑135 (MQ=255)
                                                                                     cAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTGTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACCGGCCGATGCCCTGGAGGCGGCGCCAAAGGTTTGGCGGCTGCGGCTtg            >  6:293660/1‑136 (MQ=255)
                                                                                               agcagcCCGCGCCAGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCa  <  1:230116/136‑1 (MQ=255)
                                                                                               agcagcAGGCCCCCGGCCGGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCa  <  1:335940/136‑1 (MQ=255)
                                                                                                                 |                                                                                                                     
TCTTGCCCATGCCCGCCGGGCCGACCATGGCGATCACCCCACCCTCTTCGATCGGCTCGATTTCAGGCACTTCGATCATCCGTGCCAAGTGAGCCAGCAGCATGCGCCAGGCCTGGCGCGGCTCTTCGATCTCGGCGGTCAGGTCCAGCAGCTCGCGGGCGATCGGCCCGGACAGGCCGATGCGCTGCAGGCGGCGCCAAAGGTTGGCCTGCTGCGGCTTGCTGCCCTGCA  >  ACB143/4927077‑4927307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑