breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 44,301 (T)9→8 intergenic (+132/‑359) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR
RA 120,983 (T)9→8 intergenic (+280/+92) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB
RA 179,484 T→C C316R (TGC→CGC)  CJ019_00182 → hypothetical protein
RA 690,144 A→G intergenic (+21/‑2438) bauC → / → PP_RS08750 Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
RA 1,311,935 G→T D36Y (GAT→TAT)  aaeA_2 → p‑hydroxybenzoic acid efflux pump subunit AaeA
JC 2,191,046 +CTGAAAATATCTCTTAT intergenic (‑1/‑313) PP_RS10045 ← / → purU_3 LysR family transcriptional regulator/Formyltetrahydrofolate deformylase
MC JC 4,195,579 Δ105,043 bp [CJ019_03944][CJ019_04036] 91 genes
RA 4,627,056 C→T G100D (GGC→GAC)  gacA ← Response regulator GacA
RA 5,386,500 C→A intergenic (‑509/+1142) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,503 C→G intergenic (‑512/+1139) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,506 1 bp→GT intergenic (‑515/+1136) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,507 +C intergenic (‑516/+1135) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,510 2 bp→TA intergenic (‑519/+1131) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,513 +AA intergenic (‑522/+1129) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,514 T→C intergenic (‑523/+1128) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,517 G→C intergenic (‑526/+1125) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,519 G→A intergenic (‑528/+1123) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,811 C→T intergenic (‑820/+831) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,390,241 G→A G112D (GGC→GAC)  ligH → 10‑formyltetrahydrofolate synthetase
RA 5,760,598 (A)6→5 coding (781/834 nt) CJ019_05400 → hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ ACB143 87997 88052 56 19 [17] [17] 19 [CJ019_00094]–[CJ019_00095] [CJ019_00094],[CJ019_00095]
* * ÷ ACB143 329229 329369 141 20 [18] [18] 20 [CJ019_00302] [CJ019_00302]
* * ÷ ACB143 346081 346218 138 19 [18] [18] 19 [hisF]–[btuD_1] [hisF],[btuD_1]
* * ÷ ACB143 517190 517276 87 25 [11] [15] 19 tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
* * ÷ ACB143 661080 661224 145 19 [18] [18] 19 [yfhM] [yfhM]
* * ÷ ACB143 674241 674386 146 19 [18] [18] 21 [nimR_1]–[mcpH_2] [nimR_1],[mcpH_2]
* * ÷ ACB143 1149262 1149517 256 19 [18] [18] 19 [sasA_4] [sasA_4]
* * ÷ ACB143 1585631 1585699 69 19 [17] [18] 19 CJ019_01493 hypothetical protein
* * ÷ ACB143 1697624 1697754 131 19 [17] [18] 19 [CJ019_01593]–[CJ019_01594] [CJ019_01593],[CJ019_01594]
* * ÷ ACB143 1914570 1914679 110 19 [14] [18] 21 [ppsC_1]–[CJ019_01830] [ppsC_1],[CJ019_01830]
* * ÷ ACB143 2092960 2093067 108 19 [15] [7] 21 rcsC_3/CJ019_01993 Sensor histidine kinase RcsC/hypothetical protein
* * ÷ ACB143 2288860 2288927 68 19 [17] [17] 19 [CJ019_02172] [CJ019_02172]
* * ÷ ACB143 2830072 2830233 162 24 [17] [18] 19 [gabD1] [gabD1]
* * ÷ ACB143 3046197 3046345 149 19 [17] [18] 20 [rcsC_5]–[degU_2] [rcsC_5],[degU_2]
* * ÷ ACB143 3459837 3459920 84 19 [16] [17] 20 CJ019_03284 hypothetical protein
* * ÷ ACB143 3769361 3769481 121 21 [16] [16] 19 CJ019_03581/CJ019_03582 hypothetical protein/hypothetical protein
* * ÷ ACB143 3944670 3944836 167 19 [16] [17] 20 CJ019_03729/CJ019_03730 hypothetical protein/hypothetical protein
* * ÷ ACB143 4163765 4163846 82 21 [18] [16] 19 katG/leuO_2 Catalase‑peroxidase/HTH‑type transcriptional regulator LeuO
* * ÷ ACB143 4666867 4666980 114 19 [18] [7] 20 [btuB_4] [btuB_4]
* * ÷ ACB143 4732385 4732479 95 20 [18] [17] 19 [CJ019_04468] [CJ019_04468]
* * ÷ ACB143 4845743 4845798 56 19 [18] [18] 19 recR/ybaB Recombination protein RecR/Nucleoid‑associated protein YbaB
* * ÷ ACB143 4944201 4944340 140 19 [17] [18] 20 [CJ019_04642] [CJ019_04642]
* * ÷ ACB143 5482295 5482392 98 20 [18] [18] 19 purH/purD Bifunctional purine biosynthesis protein PurH/Phosphoribosylamine‑‑glycine ligase
* * ÷ ACB143 5503639 5503826 188 20 [18] [18] 21 [CJ019_05176] [CJ019_05176]
* * ÷ ACB143 5534178 5534316 139 19 [18] [18] 21 [nadE]–[CJ019_05208] [nadE],[CJ019_05208]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 3359593 =47 (0.780)96 (1.600) 53/260 0.0 62.9% intergenic (+219/‑151) pikAII_1/CJ019_03160 Narbonolide/10‑deoxymethynolide synthase PikA2,modules 3 and 4/Tn3 family transposase ISPa42
?ACB143 4740816 = 66 (1.100)coding (903/1665 nt) CJ019_04477 Electron transfer flavoprotein‑ubiquinoneoxidoreductase
* ? ACB143 = 337660553 (0.880)167 (2.780) 63/260 0.0 74.4% coding (501/546 nt) CJ019_03184 hypothetical protein
?ACB143 = 4850748 62 (1.030)coding (435/489 nt) CJ019_04550 hypothetical protein