New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 = 475943185 (1.080)4 (0.050) 4/258 7.9 5.1% intergenic (+46/‑94) fpvA_3/ligM_KToptimized Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440
?ACB143 4760973 = 64 (0.820)intergenic (+33/+22) ligM_KToptimized/lip2 sequence generated by OPTIMIZER for KT2440/Lipase 2
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/4759295‑4759431
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGC  >  ACB143/4760973‑4761045
                                                                                                                                                                                                                  
CCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT                                                                           <  1:207344/137‑1
CCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT                                                                           <  5:468097/137‑1
   GGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCAT                                                                        <  4:214101/137‑1
              cCAACAACTCCTGGAGCCCGTACGACGTGTATTGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATAGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAA                                                             <  7:192630/136‑1
                                                     CACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACC                      >  7:358743/1‑137
                                                                         GCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGC  >  2:134447/1‑137
                                                                               TACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT                                                                           <  1:417334/58‑1
                                                                               TACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT                                                                           >  2:417334/1‑58
                                                                                                 GACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT                                                                           <  1:57050/40‑1
                                                                                                 GACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT                                                                           >  2:57050/1‑40
                                                                                                                                                                                                                  
CCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  ACB143/4759295‑4759431
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGC  >  ACB143/4760973‑4761045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.