| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,433,087 | 0 | A | C | 52.9% | 0.6 / 10.4 | 17 | intergenic (‑364/‑295) | yveA/CJ019_01212 | Aspartate‑proton symporter/IS110 family transposase ISPpu9 |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base C (9/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTT > ACB143/1432953‑1433176 | aaGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCgcagc > 8:371368/1‑137 (MQ=255) gggTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCAGTGGcgccgcca > 8:135887/1‑135 (MQ=255) tttGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGGCGGGGCGCAAACa > 6:49052/1‑137 (MQ=255) tttGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCGGCGCAGTCGGGCCGCCAACa > 2:297040/1‑137 (MQ=38) gagagaTGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCGGTCCCGGCGCAAACaagt > 4:415773/1‑134 (MQ=37) cAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGGGCGGTCGGGCCGCAAAAAAGACGTGTaag > 5:374479/1‑135 (MQ=37) cAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCGTCCGGGCTGCGCTGTAGGGCCACAAAAAGGAGGTGTaag > 4:136443/1‑135 (MQ=37) tAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGGGCGGTCGGGGAGCAAACATTAGGTGTACGGCttgg > 6:219704/1‑135 (MQ=25) ccGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTAGCGCAGCAAAAATGATGTGGACGGCGTGAGttgc > 2:260847/1‑135 (MQ=25) ggCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGc < 3:405378/137‑1 (MQ=255) gCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCaaa > 2:83552/1‑110 (MQ=255) gCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCaaa < 1:83552/110‑1 (MQ=255) gCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCGGCGCGGTCGCGCCGCCAACAAGAGGTGGACGGCGTGAGTTCCGcc > 5:164463/1‑136 (MQ=25) cgcgGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGc < 7:135887/137‑1 (MQ=255) aGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAaaaagaaaag < 8:340753/137‑1 (MQ=255) gTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGGGCGGTGGGGGCGCCAACATTAGGTGTTAGGGGTGAGTTTCGCACCGCAGCGGGCATTTTGTAAAAAAAAAAGc > 4:168094/1‑137 (MQ=12) ggCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCGGTAGCGCCGCAAACATTAGGGGTTAGGGGTGAGTTCAGCCGCGCCGCGGACAGTTTTTAAAATAAAAAGCGGtt > 5:377993/1‑137 (MQ=12) gcAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTGGCGCCGCCAACATGAGGTGTTCGGCTTCAGTTACGCAGCGCCGCTGGCATTTTTAAAAAGAAAATGCGGtttgg > 2:69725/1‑134 (MQ=17) gggCCCTGTAGGAGATCACCCAGCCCTTCGGGGCTGCGCTGTCGCGCAGCAAACATGAGGGGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAATAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCtt < 7:276361/137‑1 (MQ=9) | AAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTT > ACB143/1432953‑1433176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |