Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,134,5500TG33.3% 49.0 / 10.6 54L143V (TTG→GTG) CJ019_02952hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/15);  new base G (18/0);  total (39/15)
Fisher's exact test for biased strand distribution p-value = 8.76e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGAT  >  ACB143/3134415‑3134686
                                                                                                                                       |                                                                                                                                        
cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGtt                                                                                                                                         <  5:232960/137‑1 (MQ=255)
cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGtt                                                                                                                                         >  3:275547/1‑137 (MQ=255)
cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTACACCATTCATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACCTAGCGGCCCGCCGCGCGtg                                                                                                                                         >  3:190629/1‑136 (MQ=255)
     tACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCg                                                                                                                                    >  5:231668/1‑137 (MQ=255)
     tACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTGGGCCg                                                                                                                                    >  4:130406/1‑137 (MQ=255)
            aGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCCCGCGGTGGGCGCCCAGcg                                                                                                                             >  3:225420/1‑136 (MQ=255)
             gCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGCACAGCTg                                                                                                                            >  7:408580/1‑137 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACCGCAggg                                                                                                                          >  3:22696/1‑136 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGt                                                                                                                          >  7:343031/1‑137 (MQ=255)
                tGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACACCAGGTg                                                                                                                         >  8:425328/1‑137 (MQ=255)
                  aTCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACCCCACCTCGGACAACGTCGCCGCCCGCCGCGCCGGGGCCGCACAGCTGGGgcg                                                                                                                       >  1:188199/1‑137 (MQ=255)
                  aTCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCCGCCGGCCGCGCGGTGGCCCACCAGCTGGTgcg                                                                                                                       >  3:429703/1‑137 (MQ=255)
                     gACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGcgcgg                                                                                                                    >  6:199760/1‑136 (MQ=255)
                     gACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGcgcgc                                                                                                                    >  2:181543/1‑136 (MQ=255)
                     gACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCCGCCGCGCGGGGGCCGAAACGCTGGTGcgcgc                                                                                                                    >  1:295797/1‑136 (MQ=255)
                            ggCCCACGCGCATCCAGGCAGCAGGTATCCCTCTTTCCACATTTTTTGTCCCCTCGCTGTACAGCCTCAACACCCCCCCCGCGGCCACGGCGGCGGCGGGCGGCGCGTTGGCGGAACAGCTGGTGCGCGACACcggc                                                                                                             <  8:466647/137‑1 (MQ=255)
                               tCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGTGCGCCACCggcggc                                                                                                          >  6:445122/1‑137 (MQ=255)
                               tCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCCGCCGCGCGGTGGCCGCACAACGGGTGCGCGCCACcggcggg                                                                                                          >  1:220795/1‑136 (MQ=255)
                                cAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGACACCggcggcc                                                                                                         >  2:488203/1‑136 (MQ=255)
                                  aGCGCATCCAGGCAGCAGGTATCCAGCTTTTCACCATTGATGTGCCATCGCAGTAAAGACTCAACAAAACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGTCAAg                                                                                                       >  6:207123/1‑137 (MQ=255)
                                    cgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGCCACCGGCGGCCAggg                                                                                                     >  5:339323/1‑137 (MQ=255)
                                     gcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACCCCGGCGGCAAGGGc                                                                                                    >  6:107328/1‑137 (MQ=255)
                                     gcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGACCAGCTGGTGCGCGGCCCCGGCGGCAACGGc                                                                                                    >  3:401990/1‑137 (MQ=255)
                                     gcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCCTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGc                                                                                                    >  7:420950/1‑137 (MQ=255)
                                      aaTCCTGGCAGCAGGTATCCGGCTTTACACCATTTATGTGCCATGGCAGTACAGCCTCAACAACACCCCCGCGGACACCGGCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGcc                                                                                                   <  2:128628/136‑1 (MQ=255)
                                         ccAGGCAGCAGTTATCCCGCTTTTCACCATTGATGTCCCAACGCCGAACCCCCACAACACCCCCCCCGCGCACACCGCCGCGGCCGCCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCa                                                                                                <  6:339323/137‑1 (MQ=255)
                                          cAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACAACCTCGGACAACCTCGCGGACGGCCGCGCGGTGGCCGACCAGCAGGTCCGCGGCCACCGCGGCAACGGCCGCac                                                                                               >  8:55465/1‑136 (MQ=255)
                                           cggCAGCCGGAATCCCGCTTTGCACCATGGAGGTGCCTTCGCATTACAGCCGCAACAACCCCCCCTCGGACACGGTCGCGCCGGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATc                                                                                              <  2:278890/136‑1 (MQ=255)
                                                  tgttcacccGCTTTCCCCCATTGAGGTGCCACCGCACTACACCCTCACCAACCCCCCCTGGCCCACGGGCGGGGCCGGCCGCGCGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgtgt                                                                                       <  4:391538/131‑1 (MQ=255)
                                                    gTATCCCGTTTTTCACCTTTTAGTTGCCATCGAATTACCGCCTCAACAACCCCACCTCGCACAACGGCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTc                                                                                     <  6:386181/137‑1 (MQ=255)
                                                    gTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGGCGGACACCTCGTGGGCGCCCCCCGCGGCGAGGGCCGCCTCCAGgtggtt                                                                                     >  3:416634/1‑134 (MQ=255)
                                                    gTAGCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACAACCGCGTACACCGGCGCGGCCGGCGGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTc                                                                                     <  8:369128/137‑1 (MQ=255)
                                                              tttCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGCACACCGGCGCGCCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCt                                                                           <  8:90173/137‑1 (MQ=255)
                                                                         aTGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGCACAGCTGGTGCGCGCCACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCg                                                                >  8:40069/1‑137 (MQ=255)
                                                                            tgCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGGCGCACGGCTGGTGCGCGACACCGGCGGCCAGGGCCGCATGCGGGTGTTCAACGGCTTCTTCGGGGTggccggg                                                             >  7:337575/1‑131 (MQ=255)
                                                                               aaTCGCAGTACCGCCTCAACAACACCCCCTCGGACACCGGCGCGGCGGGCGGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                          <  8:108056/136‑1 (MQ=255)
                                                                                 tCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACACCTGGTGCGCGACACCGGCGGCAAAGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGc                                                        >  5:386845/1‑137 (MQ=255)
                                                                                    cAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGCCCGCGCGGTGGGCGAACACCTGGTGCGCGACACCGGCCGCAAGGGCCGCATGCTGGTGTTCAAAGGGGTCTAATGGGTGCCCGGCTGTGCGaa                                                     >  2:16406/1‑136 (MQ=255)
                                                                                         cAGCCTCAACAACACCACCTCGGACAACGTCGCGGACGGCCGCGCGTTGGCCGAACACCAGGTGCGCGACACCGGCGGCAACGGCCGCATCCTGGTGTTGAACGGCTTCTTCGGGGTGCCCGTCTGTGGGATCCGcg                                                >  8:292945/1‑136 (MQ=255)
                                                                                           gCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGCACAGCTGGTGCGCGCCACCGGCGGCCAGGGCCGCATCCGGGTGTTCAACCGCGTCTACGGGGTGGCGGGCTGTGCGAGCCGCGac                                              >  4:237071/1‑137 (MQ=255)
                                                                                           cccTCACCAACACCCCCTCGCACACCGGCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTac                                              <  8:96703/136‑1 (MQ=255)
                                                                                               caacaaCACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGCACAGCTGGTGCGCGACCCCGGCGGCAAGGGCCGCATCCTGGGGTGCAACGGCTTCTACGGGGTGCCGGGCTGTGCGATCCGCTacgacg                                          >  8:107894/1‑137 (MQ=255)
                                                                                               caacaaCACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGACCAGCTGGTGCGCGACACCGGCGTCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTTTGCGATCAGCTacgacg                                          >  8:100740/1‑137 (MQ=255)
                                                                                               caacaaCACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACCCCGGCGGCAAGGGCCGCATCCTGGGGGTCAACGGCGTCGACGGGGTGCCGGTCTGTGAGAGACGCTcagacg                                          >  5:389186/1‑137 (MQ=255)
                                                                                                  caacaCCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCCAGGGCCGCATCCTGGTGTTCAACGGCGTCTTCGGGGTGGCGGTCTGGGCGATCCGCGACGACGAGt                                       >  5:280613/1‑137 (MQ=255)
                                                                                                    acccccccTCGGCCAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTg                                     <  8:343031/131‑1 (MQ=255)
                                                                                                    acacCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTg                                     >  8:361516/1‑137 (MQ=255)
                                                                                                    acacCACCTCGGACAACGTCGCGGCCCGCCGCGCGTTGGCCCAACAGCAGGTTCGCGACACCGGCGGCAAGGGCCGCAACCTGGTGTTCAAACGCTTCTACCGGGTGCCGGTCGGTTCGATCCCCTACCCCGAGTTg                                     >  8:204324/1‑137 (MQ=255)
                                                                                                        caccTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCCAGGGCCGCATCCTGGTGTTCAACGGCGTATACGGGGTGCCGGGCTGTGCGCTCCGCTACGACGAGTTgaaga                                 >  7:420356/1‑137 (MQ=255)
                                                                                                             cGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTAAACGGCGTCTACGGGGTGCCGGTCTGTGGGATCCGCGACGACGAGTTGAAGAAGGtt                            >  2:133563/1‑136 (MQ=255)
                                                                                                             cGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTa                            >  7:272275/1‑137 (MQ=255)
                                                                                                                    ggtgggggcgggcgGGGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCg                     <  8:420356/131‑1 (MQ=255)
                                                                                                                    cggcgcgCCCGCCGGCGCGTGGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCg                     <  3:422080/134‑1 (MQ=255)
                                                                                                                       cgcgGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGCCACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGGGCCGGTCTGTGCGATCCGCTACGCCGAGTTGAAGAAGGTACTGGCCGatc                  >  6:88711/1‑137 (MQ=255)
                                                                                                                        gcgGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCGGGTGTTGAACGGCTTCTACGGGGTGCCGGGCTGTGCGATCCGCTACGCCGAGTTGAAGAAGGTACTGGCCGgtca                 >  7:241527/1‑137 (MQ=255)
                                                                                                                         cgccgggccgGGCTTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcat                <  4:225420/132‑1 (MQ=255)
                                                                                                                              ggccgGGGGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCa           <  4:67153/137‑1 (MQ=255)
                                                                                                                                       ttGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGAt  <  4:416634/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
CTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGAT  >  ACB143/3134415‑3134686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑