Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,425,0700GC39.5% 41.7 / 34.1 43intergenic (‑29/‑89)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/3);  new base C (0/17);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 2.71e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCCTTGCCTGCC  >  ACB143/4424935‑4425202
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tgCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTGCATCCTCGGGGTCGCAAAAAAGAAgg                                                                                                                                     >  8:97976/1‑137 (MQ=255)
tgCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAgg                                                                                                                                     >  2:32494/1‑137 (MQ=255)
   gAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGa                                                                                                                                  >  7:412129/1‑137 (MQ=255)
   gAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGa                                                                                                                                  >  7:33724/1‑137 (MQ=255)
            tGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCAc                                                                                                                         >  5:501015/1‑137 (MQ=255)
                      gCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCAt                                                                                                               >  8:267719/1‑137 (MQ=255)
                             gccACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGt                                                                                                        >  6:326554/1‑137 (MQ=255)
                                                      caaccacaACCGCCCACCAGCACCCCCCCAGCCAACGGAAGAATCCCACCCAGGTCCCACCCTGGGGGGCGCAAAAAAGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTc                                                                               <  6:364988/133‑1 (MQ=255)
                                                         ccacaCCCGCCCACCATCACCCCCCCCCCCACCGCCATACTCCCACGCATTCACCAGCCTGGGGGACCAAAAAACCACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCa                                                                            <  1:95614/136‑1 (MQ=255)
                                                                cGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATaaaa                                                                     >  4:236913/1‑137 (MQ=255)
                                                                  gCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACa                                                                   >  1:409187/1‑137 (MQ=255)
                                                                     aGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGc                                                                >  7:212873/1‑137 (MQ=255)
                                                                              aCCGCAGCCAACGGCAGAATCCCACGCTTGTACCATCCTCGGGGTCGAAAAAAAGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAg                                                       <  7:267719/137‑1 (MQ=255)
                                                                                  cAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAAc                                                   >  7:196378/1‑137 (MQ=255)
                                                                                     ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca                                                >  1:165171/1‑137 (MQ=255)
                                                                                     ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca                                                >  6:429477/1‑137 (MQ=255)
                                                                                     ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCAAGAGGAACCca                                                >  8:48911/1‑137 (MQ=255)
                                                                                        aCGCCATAATCGACCGCTTGTTCCATCCGCGGGGTCGAAAAAAACACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCcatca                                             <  5:374084/137‑1 (MQ=255)
                                                                                         gggCATAATCGCACGCATGTTCCAGCCTGGGGGCCCAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCcatcat                                            <  8:212873/136‑1 (MQ=255)
                                                                                               attCGCCCGCATGTTCAAAGGTGGGGTTCGAAAAAACGATCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                                      <  3:438824/135‑1 (MQ=255)
                                                                                               aaacgcactcaTGTTCCATCCTGGGGGCGTCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                                      <  8:455993/134‑1 (MQ=255)
                                                                                               aaTCGCCCGCTTGTTCCACCCGCGGGGGCACAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                                      <  1:148710/137‑1 (MQ=255)
                                                                                               aaTCGCACTCTTGTACCATCCTGGGGGTCTAAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                                      <  6:501015/137‑1 (MQ=255)
                                                                                               aaTCCCACGCTTGTCCCATCCTCGGGCGCCAAAAAAATCCCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                                      <  5:34385/137‑1 (MQ=255)
                                                                                               aaTCCCACCAATGTACAATCCTGGGGTTCGAAAAAAACCACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt                                      <  1:142276/137‑1 (MQ=255)
                                                                                                aTCGCACGATTTTTCCACCCTGGGGCTCTAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTg                                     <  1:165901/137‑1 (MQ=255)
                                                                                                 ccgcccgctTGTTCCATCCTCGGGGGAGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGc                                    <  5:329506/136‑1 (MQ=255)
                                                                                                       gcaTGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATc                              >  1:246634/1‑137 (MQ=255)
                                                                                                             tCCAGCCTCGGGGTCTAAAAAACCACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTAc                        <  8:456367/137‑1 (MQ=255)
                                                                                                                 tCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgc                    >  2:429116/1‑137 (MQ=255)
                                                                                                                 cccgcGGGGTCCAAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgc                    <  3:100124/133‑1 (MQ=255)
                                                                                                                  ccgcGGGGGCCCAAAAAACAACGCGACTCTAGCACTGGCAGGCATCACGAGGGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc                   <  8:108994/134‑1 (MQ=255)
                                                                                                                  ccTGGGGGTCCCAAAAAAGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc                   <  7:466840/137‑1 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc                 >  7:456367/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc                 >  7:314982/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc                 >  3:334180/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc                 >  2:469028/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc                 >  8:373251/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc                 >  3:449982/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc                 >  8:474122/1‑137 (MQ=255)
                                                                                                                    tCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgccgc                 >  2:4193/1‑137 (MQ=255)
                                                                                                                       gggTCGCAAAAAAGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCt              <  6:234536/137‑1 (MQ=255)
                                                                                                                        ggTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtg             >  2:270059/1‑137 (MQ=255)
                                                                                                                             aaaaaaaGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgccttg        <  4:452965/136‑1 (MQ=255)
                                                                                                                             aaaaaaaGAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtgccttg        <  8:33724/136‑1 (MQ=255)
                                                                                                                                   agaacGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCCTtgcctgcc  <  8:412129/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                    
TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAAGAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTGCCTTGCCTGCC  >  ACB143/4424935‑4425202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑