Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,768,3280TG31.9% 83.7 / 13.5 71E610A (GAG→GCG) caiTL‑carnitine/gamma‑butyrobetaine antiporter
Reads supporting (aligned to +/- strand):  ref base T (10/37);  new base G (22/0);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.10e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTG  >  ACB143/5768198‑5768461
                                                                                                                                  |                                                                                                                                      
aTCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctcgctcg                                                                                                                                  <  3:21850/137‑1 (MQ=255)
aTCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctcgctcg                                                                                                                                  <  8:231568/137‑1 (MQ=255)
aTCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctcgctcg                                                                                                                                  <  2:186281/137‑1 (MQ=255)
aTCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctcgctcg                                                                                                                                  <  6:224323/137‑1 (MQ=255)
aTCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctcgctcg                                                                                                                                  <  1:27263/137‑1 (MQ=255)
aTCGAGCACATCACGCACGATCTGGGCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCctcgctcg                                                                                                                                  <  5:42605/137‑1 (MQ=255)
 tCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCgg                                                                                                                                 <  1:259657/137‑1 (MQ=255)
 tCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCgg                                                                                                                                 <  1:81195/137‑1 (MQ=255)
 tCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCgg                                                                                                                                 <  2:40568/137‑1 (MQ=255)
 ccGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCgg                                                                                                                                 <  1:465221/136‑1 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGGGGGGCGCTCGgcg                                                                                                                               >  1:30260/1‑137 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGGCGCGCGCgggg                                                                                                                               >  4:21850/1‑135 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCgcggcg                                                                                                                               >  2:96589/1‑137 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCgcggcg                                                                                                                               >  2:387822/1‑137 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGgcg                                                                                                                               >  4:24097/1‑137 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTCGgcg                                                                                                                               >  7:372911/1‑137 (MQ=255)
   gAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGACTTCATGAAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTAAGGCTCACCCTCGACCGGCGCCGAGATCGgcg                                                                                                                               >  2:93027/1‑137 (MQ=255)
        caTCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTCGGCGCGGcc                                                                                                                          >  6:127789/1‑137 (MQ=255)
          tCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCat                                                                                                                        <  5:297313/137‑1 (MQ=255)
          tCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCat                                                                                                                        <  6:228220/137‑1 (MQ=255)
          acacgcacgATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCat                                                                                                                        <  1:423318/136‑1 (MQ=255)
                      tggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGCCCGGGGGGGCGCGCGGCGCGGCCCGATAGCCGACAAg                                                                                                            >  7:83690/1‑137 (MQ=255)
                      tggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAg                                                                                                            <  6:219269/137‑1 (MQ=255)
                      tggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAg                                                                                                            <  1:120352/137‑1 (MQ=255)
                      tggtctggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTGGGCGGGGCCCTAGAGCCGGCAAg                                                                                                            >  4:378187/1‑137 (MQ=255)
                           tggGGCAAAGCCCATCAGGTCGTACTTCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCGCGAGCAGTCAAGGCTCa                                                                                                       >  5:104041/1‑137 (MQ=255)
                           tggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGGGGCGCGGGGGGGGCGGCCCGAGAGCCGTCGAGGCtgc                                                                                                       >  5:58527/1‑135 (MQ=255)
                           tggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGGCGCGCGCGGCGCGGCGCGATATCCGTCACGGGTcc                                                                                                       >  7:144706/1‑136 (MQ=255)
                           tggGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCCGATAGCCGGCAAGGCTcc                                                                                                       >  5:371360/1‑136 (MQ=255)
                                cAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCTATAGGCGGCAAGGCTGCCTTCg                                                                                                  >  2:460776/1‑137 (MQ=255)
                                 aaaGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCACGCTCGGCGCGGCCATATAGCCGTCAACGCGCAcgtcgt                                                                                                 >  8:89837/1‑137 (MQ=255)
                                    gCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTTGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGa                                                                                              <  3:163442/137‑1 (MQ=255)
                                    gCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGa                                                                                              <  2:292339/137‑1 (MQ=255)
                                    gCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGa                                                                                              <  2:133636/137‑1 (MQ=255)
                                     cccATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCGCCGCGCGGGCAGAAAGCCGTCAAGGCGCACATCGTAGaa                                                                                             >  6:440588/1‑136 (MQ=255)
                                     cccATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCCTATAGACGTCAAGGCTCCCAGCGTAGAc                                                                                             >  1:14070/1‑137 (MQ=255)
                                      ccATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGGGGCGCGCGGGGCGCGGCCCGAGAGCCGGCACGGCGAACGTGGTAGACg                                                                                            >  4:228091/1‑137 (MQ=255)
                                            ggTCGTACTCCTGACTGCCGTCATGCACGTAGACATCGGCGCGGTAGAACCGCTGGGGCAGTTCAGGGTCAACCTCGACCGGCGCCACGCTCCGGGCCGCCAAATAGACCTGAACGCTCCCATCGGAGACgccacgg                                                                                      >  4:88068/1‑131 (MQ=255)
                                              tCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                    <  3:323419/137‑1 (MQ=255)
                                              tCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                    <  2:229875/137‑1 (MQ=255)
                                              tCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGtt                                                                                    <  3:385401/137‑1 (MQ=255)
                                                gTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGGCGGGGGGGGGGGGGGCCGATAGCCGTCGCGGCGCACAGAGAAGACGAACGGGtggg                                                                                  >  5:371402/1‑134 (MQ=255)
                                                gTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGc                                                                                  <  6:341796/137‑1 (MQ=255)
                                                  aCTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCCCCGCGCGGCCCGAAAGACGGCAACGCTCCCAACGGAGAAGAACGGCATGccc                                                                                >  2:10055/1‑137 (MQ=255)
                                                   cTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCct                                                                               <  7:248328/137‑1 (MQ=255)
                                                   cTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCct                                                                               <  6:20603/137‑1 (MQ=255)
                                                   cTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCGGGCGCAGTGCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCct                                                                               <  2:235722/137‑1 (MQ=255)
                                                         aCTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCATATATCCGTCAAGGCTCACGTAGTTGACGCACGGGTTGGCCGCTTCca                                                                         >  2:415940/1‑137 (MQ=255)
                                                           tGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTCGGCGCGGGCATTTAGACGTCAACGCGCACTTCGTAGACGCACGGGGTGCCCTCTTCcatg                                                                       >  2:175672/1‑136 (MQ=255)
                                                                 cATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTAcatcaaaacc                                                                 >  2:361676/1‑132 (MQ=255)
                                                                           gACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGCACGGGGTGCCCTCGTACAACAT‑AAACCCCAAGcccc                                                      >  8:428712/1‑136 (MQ=255)
                                                                                   cgcgGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTCGGCGCGGCCATATAGCCGTCAATGCTCACTTCGTAGACGAACGGGTTGCCCGCTTCCATCATACACCC‑CACGCCCATAAGGttt                                              >  4:16567/1‑136 (MQ=255)
                                                                                     cgGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCTAAAGCCCATCAGGTTgcg                                            <  1:10055/137‑1 (MQ=255)
                                                                                     cgGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTgcg                                            <  5:81253/137‑1 (MQ=255)
                                                                                     cgGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTgcg                                            <  4:175744/137‑1 (MQ=255)
                                                                                     cgGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTgcg                                            <  5:423611/137‑1 (MQ=255)
                                                                                     cgGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCGCGCTCGGCGCGGCCCTATAGCCGTCAAGGCTCACTTCGTTGAGGAACGGGTTGCCCGCTTGCATCAT‑CACCCGCAAGCCCAGCAGGTTgcg                                            >  6:482576/1‑137 (MQ=255)
                                                                                                       aGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGt                          <  3:378187/137‑1 (MQ=255)
                                                                                                              gCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCAcc                   <  6:104041/137‑1 (MQ=255)
                                                                                                               cTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCt                  <  6:371402/137‑1 (MQ=255)
                                                                                                                tCACCCTCGACCGGCGCCGCGCGCGGCGCGGCCATATAGCCGGCAACGCTCACTTCGGAGACGAACGGGGGGGCCGCTGCCATCAT‑CACCCCCAACCCCCTCAGGGTTCGCGACTGGCCAACGCGGGGTTGCAACTc                 >  8:396740/1‑137 (MQ=255)
                                                                                                                     cTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  3:228091/137‑1 (MQ=255)
                                                                                                                     cTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  5:232833/137‑1 (MQ=255)
                                                                                                                     cTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  1:481668/137‑1 (MQ=255)
                                                                                                                     cTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  1:198517/137‑1 (MQ=255)
                                                                                                                     cTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  4:355794/137‑1 (MQ=255)
                                                                                                                     cTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  1:255636/137‑1 (MQ=255)
                                                                                                                     cTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGc            <  1:415940/137‑1 (MQ=255)
                                                                                                                      tCGACCGGCGCCTCGCTCGGCGCGGCCAAATAGCCGTCAAGGCTCACTTGGTAGACGCACGGGGTGGCCTCTTGCATCAT‑CACCCGCAAGCCCATCAGGTTGGGCGCCTGGCCACCGCGGGGTTGCACCTCGAAGcc           >  4:324561/1‑137 (MQ=255)
                                                                                                                      tCGACCGGCGCCTCGCTCGGCGCCGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGGCCGCGTCCATCAT‑CCACCGCAAGCCCATCACGTTGGGCGCCTGGGCACCGCGGGGTTCCCCCTCGCAGcc           >  4:46485/1‑137 (MQ=255)
                                                                                                                      tCGACCGGCGCCTCCCACGGCCCGGCCATAAACCCGTCAAGGCTCACTTCGTATACGAACGGGGAGCCGACATACCTCAT‑GAACCGCAAGCCCCTCACGATTCGCGACTCGCCAACGCCGGGTTACACCTCGAAGcc           >  6:301889/1‑137 (MQ=255)
                                                                                                                      tCGACCGGCGCCGCGCTCGGCGCGGCCCAATATCCGTCAAGGCTCACTTCGGAGACGAACGGGTTGCCCGCTTCCATCCT‑AACCCGCAAGCCCATAAGGTTGCGGGCCTGGCCAACGCCGGGTTGCACAGCGAAGcc           >  8:470872/1‑137 (MQ=255)
                                                                                                                      tCGACCGGCGCCGCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGGTGGCCACGTGCATCAT‑CACCCGCAAGCCCCTCAGGTTGGGCGCCTGGCCCACGCCGGGTTCCACCACGAAGcc           >  2:335629/1‑137 (MQ=255)
                                                                                                                       cGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGccc          <  5:71080/137‑1 (MQ=255)
                                                                                                                            ggCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGt     <  2:74452/137‑1 (MQ=255)
                                                                                                                            ggCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGt     <  6:466528/137‑1 (MQ=255)
                                                                                                                               gcCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTg  <  4:56871/137‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                      
ATCGAGCACATCACGCACGATCTGGTCTGGGGCAAAGCCCATCAGGTCGTACTCCTGACTGCCGTCATGCAGGTAGACCTCGGCGCGGTAGAAGCGCTGGCGCAGTTCAGGCTCACCCTCGACCGGCGCCTCGCTCGGCGCGGCCATATAGCCGTCAAGGCTCACTTCGTAGACGAACGGGTTGCCCTCTTCCATCAT‑CACCCGCAAGCCCATCAGGTTGCGCGACTGGCCAACGCGGGTTTCCACCTCGAAGCCCTGCGTCTG  >  ACB143/5768198‑5768461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑