| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 4,759,606 | G→A | A28T (GCC→ACC) | ligM_KToptimized → | sequence generated by OPTIMIZER for KT2440 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,759,606 | 0 | G | A | 98.7% | 275.5 / NA | 79 | A28T (GCC→ACC) | ligM_KToptimized | sequence generated by OPTIMIZER for KT2440 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (50/28); minor base C (1/0); total (51/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTAAAGACGCTC > ACB143/4759472‑4759738 | aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCAcc > 7:388489/1‑137 (MQ=255)aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCAcc > 8:182305/1‑137 (MQ=255)aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCAcc > 7:136036/1‑137 (MQ=255)aaTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCAcc > 3:269371/1‑137 (MQ=255) aTTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCt > 6:281234/1‑137 (MQ=255) ttGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTa < 6:93993/137‑1 (MQ=255) gAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTc > 1:106607/1‑137 (MQ=255) gCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGTTGCTGGCCGCCGGCGGCACCCCCGTTGAAATGTTGCGTACGTCCAAGATTGGCACCTACGTCTa > 7:218996/1‑137 (MQ=255) gCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTa > 7:60137/1‑137 (MQ=255) ggATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTAcc > 1:60556/1‑137 (MQ=255) gATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTAccc < 2:101957/137‑1 (MQ=255) aTAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCg > 5:422216/1‑137 (MQ=255) tAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCgg < 2:393254/137‑1 (MQ=255) cacCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCa > 6:211272/1‑137 (MQ=255) acCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCCCCTACGTCTACCCGGTCGACGCGCCag > 2:189965/1‑137 (MQ=255) acCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCag > 5:21904/1‑137 (MQ=255) cacaTAGGGTTTAGGAGGTATACATGAGAGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCAGGTCGTCTCGCCAGAGtac > 5:170706/1‑135 (MQ=255) caTAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTtctc > 4:160964/1‑137 (MQ=255) ggTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACt < 5:260798/137‑1 (MQ=255) gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTg > 5:458169/1‑137 (MQ=255) ttAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGc > 7:357021/1‑137 (MQ=255) tAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCg < 4:110556/137‑1 (MQ=255) ggaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa < 5:391091/137‑1 (MQ=255) gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTAc > 7:489596/1‑137 (MQ=255) gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTAc > 2:472468/1‑137 (MQ=255) gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTAc > 8:390438/1‑137 (MQ=255) tataCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa < 8:269878/137‑1 (MQ=255) taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACa > 5:358152/1‑137 (MQ=255) taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACa > 6:201899/1‑137 (MQ=255) taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACa > 7:359786/1‑137 (MQ=255) taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACa > 2:12526/1‑137 (MQ=255) taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACa > 7:253187/1‑137 (MQ=255) cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGt > 1:98309/1‑137 (MQ=255) cgcCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCtt > 2:116252/1‑137 (MQ=255) gcCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTcgcg < 4:100368/99‑1 (MQ=255) gcCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTcgcg > 3:100368/1‑99 (MQ=255) gcCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTg < 8:8862/137‑1 (MQ=255) ccccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGgtcg > 2:51836/1‑93 (MQ=255) ccccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGgtcg < 1:51836/93‑1 (MQ=255) ccccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg > 1:58797/1‑137 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCATAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgc > 5:495754/1‑137 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTggg > 1:283322/1‑136 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgc > 5:161693/1‑137 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgc > 4:473562/1‑137 (MQ=255) cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGGTCTCTAACTGGCGTCCCGAACAGTGGGCTTGgc > 5:464697/1‑137 (MQ=255) cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcgc > 6:437356/1‑137 (MQ=255) cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcgc > 5:342854/1‑137 (MQ=255) caCTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGGCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCa < 5:22257/136‑1 (MQ=255) cAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCt > 8:328600/1‑137 (MQ=255) cAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGGACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCt > 5:286258/1‑137 (MQ=255) aGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTg > 2:26987/1‑137 (MQ=255) aGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTg > 7:18267/1‑137 (MQ=255) cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGa > 2:30582/1‑137 (MQ=255) cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGa > 2:405360/1‑137 (MQ=255) cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGa < 7:70144/137‑1 (MQ=255) cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGa > 1:57226/1‑137 (MQ=255) ccgccgGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCaa < 4:428305/137‑1 (MQ=255) cgccgGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCaaa > 3:340658/1‑137 (MQ=255) ccgGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAAcc < 6:458169/137‑1 (MQ=255) ggcggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACcca < 1:30582/137‑1 (MQ=255) ggcggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACcca < 8:357021/137‑1 (MQ=255) ggcggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACcca < 8:388489/137‑1 (MQ=255) ggcggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACcca < 8:75970/137‑1 (MQ=255) gggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACccacc < 6:21904/136‑1 (MQ=255) cggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACccacc > 2:199464/1‑137 (MQ=255) cAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACcaca < 1:116252/137‑1 (MQ=255) cAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACcaca < 2:60556/137‑1 (MQ=255) cAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACcaca < 4:340658/137‑1 (MQ=255) cacCGTTGAAATGTTGAGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACAtgg < 8:253187/137‑1 (MQ=255) ccGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACAtggtg < 2:124302/137‑1 (MQ=255) ccGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACAtggtg < 3:160964/137‑1 (MQ=255) aaaTGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTg > 3:460683/1‑137 (MQ=255) aaaTGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTg > 4:52230/1‑137 (MQ=255) ttGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACAt < 8:489596/137‑1 (MQ=255) cGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATccc > 3:116053/1‑136 (MQ=255) aaCTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCgg > 8:463838/1‑137 (MQ=255) tcacAGATTTGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTaa < 6:495754/134‑1 (MQ=255) ttGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTAAAGACGct > 6:475427/1‑137 (MQ=255) tGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGTCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTAAAGACGctc < 1:248542/137‑1 (MQ=255) | AATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTAAAGACGCTC > ACB143/4759472‑4759738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |