Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 44,293 Δ1 bp 100% intergenic (+124/‑367) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB14344,2930T.96.6% 255.2 / ‑1.2 57intergenic (+124/‑367)oprD_1/czcR_2Porin D/Transcriptional activator protein CzcR
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base . (18/37);  minor base G (1/0);  total (20/37)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGCCAGC  >  ACB143/44172‑44424
                                                                                                                         |                                                                                                                                   
ctcTAACTGGGACTGTTATCCATTGGCGGTTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTACTTTGGTAAAAGTTGGATTTTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTaa                                                                                                                       <  2:237463/133‑1 (MQ=255)
          gCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAg                                                                                                            <  3:23677/136‑1 (MQ=255)
            cAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGc                                                                                                          <  3:200643/136‑1 (MQ=255)
                          cGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgc                                                                                            >  3:25781/1‑136 (MQ=255)
                          cGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcg                                                                                             >  6:278686/1‑135 (MQ=255)
                          cGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCGTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTAAAGTATGCGTTCGAGTGTAcg                                                                                             >  6:274237/1‑135 (MQ=255)
                           gggTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgca                                                                                           <  7:63696/136‑1 (MQ=255)
                             gTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatc                                                                                         <  5:76255/136‑1 (MQ=255)
                             gTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatc                                                                                         <  4:136112/136‑1 (MQ=255)
                               ccTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTCTGCGCC‑TATTTTTTGACTAAAATTAAAGTAGGCGTTCGATTGTAcgcctcgc                                                                                       >  3:114168/1‑136 (MQ=255)
                                cTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTAcgcatcgca                                                                                      >  1:195088/1‑136 (MQ=255)
                                      gTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                                 <  5:50331/135‑1 (MQ=255)
                                      gTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                                 <  5:75089/135‑1 (MQ=255)
                                      gTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                                 <  1:121000/135‑1 (MQ=255)
                                      gTAAAACGAGCATTACGCATTCAAGTTGAGTGTCTTAAGTGCTCCTTTTGTAAAAGGTTGATTTTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAATATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCg                                                                                 <  7:256202/135‑1 (MQ=255)
                                          aaCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGaa                                                                            <  7:181577/136‑1 (MQ=255)
                                          aaCGAGCATTAAGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTTGGCTCCTTTTTTTTT‑CCTAAAATTCAAGTAGGAGGTCGAGTGTACGCATCGCATGCCGGTGaa                                                                            >  4:267565/1‑136 (MQ=255)
                                           aCGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCGTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACTCATCGCATGCCGGTGAAg                                                                           <  2:156837/136‑1 (MQ=255)
                                             gAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTTGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                                         <  3:14874/136‑1 (MQ=255)
                                             gAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                                         <  6:301421/136‑1 (MQ=255)
                                             gAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                                         <  4:59093/136‑1 (MQ=255)
                                             gAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGtt                                                                         >  4:144252/1‑136 (MQ=255)
                                                cATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTTGCGCCCATTGGGCGCCGTTTTTTTGGCGT‑AAATTCAATTAGCCGTTGGATTGTACGCATCCCCTGCCGGTGAAGTTCAt                                                                      >  2:277340/1‑136 (MQ=255)
                                                cATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCAt                                                                      >  2:260920/1‑136 (MQ=255)
                                                 aTTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATg                                                                     <  4:242497/136‑1 (MQ=255)
                                                  ttACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                    <  3:122352/136‑1 (MQ=255)
                                                  ttACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGa                                                                    <  5:52165/136‑1 (MQ=255)
                                                  ttACGAATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGACTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGGGAAGTTCATGa                                                                    <  5:257884/136‑1 (MQ=255)
                                                     cGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTTGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑GTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACt                                                                  <  7:253205/135‑1 (MQ=255)
                                                     cGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACt                                                                  <  7:253203/135‑1 (MQ=255)
                                                      gCATTCACCTGGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTac                                                                <  7:58479/136‑1 (MQ=255)
                                                            aCCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCaa                                                           <  2:215659/135‑1 (MQ=255)
                                                            aCCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAAt                                                          >  7:192906/1‑136 (MQ=255)
                                                             ccTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAATAGTCGTTCGATTTTACGCATCGCATGGCGGTGAATTTCATGACTACACCAAt                                                          >  5:199008/1‑135 (MQ=255)
                                                                 gAGTGTCTTAAGTGGTCCTTTGGTAAAAGGTGGATATTTGGCGCCGATTTGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGGGTTCTATTGTACTCATCGCATGCCGGGGAAGTTCATGACTACACCAATGAcc                                                      <  5:220562/135‑1 (MQ=255)
                                                                 gAGTGGCTTATGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCGCATTGGGAGGC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGGATGCCGGTGAAGTTCATGACTACACCAATGTcc                                                      <  2:274990/135‑1 (MQ=255)
                                                                 gAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAcc                                                      <  2:72563/135‑1 (MQ=255)
                                                                 gAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGAc                                                       <  7:52044/134‑1 (MQ=255)
                                                                   gTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGt                                                   <  5:278686/136‑1 (MQ=255)
                                                                    tGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGt                                                   <  5:274237/135‑1 (MQ=255)
                                                                     ggCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGtct                                                 >  1:20760/1‑136 (MQ=255)
                                                                       cTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGCCCAGTctct                                               >  1:52845/1‑136 (MQ=255)
                                                                          aaGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTc                                             >  1:235736/1‑135 (MQ=255)
                                                                                    ttGGTAAAAGTTGGATATTTGGATCACATTGTGCGCC‑TTTTTTTTGCCTAAAATTAAAGTAGGCGTTCGATTGTACGCATCGCATGCAGGTGAAGTGACTGACTCAACCAATGACCAGTCTCTGCAATTTGAGCgc                                  >  6:183311/1‑135 (MQ=255)
                                                                                        tAAAAGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTc                              <  1:303835/136‑1 (MQ=255)
                                                                                          aaaGGTGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGc                            >  5:153463/1‑136 (MQ=255)
                                                                                               tGGATATTTGGCGCCCATTGGGCGCC‑TTTTTTTTGACTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTAATGACTAGACCAATTAACAGGAGCTTCTATGTGAGCGACCTCGCCGTCa                       >  8:73656/1‑136 (MQ=255)
                                                                                                   tatTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCt                    <  5:120850/135‑1 (MQ=255)
                                                                                                   tatTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTg                   <  7:240158/136‑1 (MQ=255)
                                                                                                    atTTGGCGCCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCt                    >  4:87005/1‑134 (MQ=255)
                                                                                                         gcgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg              <  7:126775/135‑1 (MQ=255)
                                                                                                         gcgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg              >  8:14169/1‑135 (MQ=255)
                                                                                                         gcgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCgg              <  6:79248/135‑1 (MQ=255)
                                                                                                         gcgcCCATTGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGa             >  2:239740/1‑136 (MQ=255)
                                                                                                                ttGGGCGCC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGc      <  8:214908/136‑1 (MQ=255)
                                                                                                                    gcgcC‑TTTTTTTTGCCTAAAATTCAATTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTATCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGCCAGc  <  7:114682/136‑1 (MQ=255)
                                                                                                                    gcgcC‑TTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGCCAGc  <  5:87974/136‑1 (MQ=255)
                                                                                                                         |                                                                                                                                   
CGCTAACTGGGCCAGTTATCCATTGGCGGGTCCTGTTAGTAAAACGAGCATTACGCATTCACCTTGAGTGGCTTAAGTGCTCCTTTGGTAAAAGGTGGATATTTGGCGCCCATTGGGCGCCTTTTTTTTTGCCTAAAATTCAAGTAGGCGTTCGATTGTACGCATCGCATGCCGGTGAAGTTCATGACTACACCAATGACCAGTCTCTTCAATGTGAGCGACCTCGCCGTCATCTGAGCGGAACGAAGCCAGC  >  ACB143/44172‑44424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑