Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,760,593 Δ1 bp 100% coding (776/834 nt) CJ019_05400 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,760,5930A.90.0% 153.5 / 7.2 40coding (776/834 nt)CJ019_05400hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (30/6);  minor base T (0/3);  total (30/10)
Fisher's exact test for biased strand distribution p-value = 9.19e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCG  >  ACB143/5760466‑5760722
                                                                                                                                |                                                                                                                                 
cAAGCGCGCGAACAGCACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGGaaaaacgcc                                                                                                                           <  6:64394/136‑5 (MQ=255)
       gcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcag                                                                                                                     >  5:95197/1‑134 (MQ=255)
       gcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcag                                                                                                                     >  2:320702/1‑134 (MQ=255)
                  cAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGCACGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTgg                                                                                                        >  6:190298/1‑136 (MQ=255)
                       cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                   >  4:219402/1‑136 (MQ=255)
                       cGACATGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAATCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGCAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                                   >  8:84980/1‑136 (MQ=255)
                        gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCTGCCAGCAGGAAGCGCTGgccgccg                                                                                                  >  8:232559/1‑136 (MQ=255)
                        gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                  >  7:130500/1‑136 (MQ=255)
                        gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                  <  3:56009/136‑1 (MQ=255)
                        gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                  >  3:144907/1‑136 (MQ=255)
                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                              >  3:231390/1‑136 (MQ=255)
                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                              >  2:107707/1‑136 (MQ=255)
                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                              >  7:46707/1‑136 (MQ=255)
                            tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                              >  6:120127/1‑136 (MQ=255)
                              gCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCCGGCCCGTGAAGCTGACGAGCCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTg                                                                                            <  3:266025/136‑1 (MQ=255)
                                 aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGccc                                                                                         >  7:277704/1‑136 (MQ=255)
                                   ggTCTGGACGACCATCGAGCAAACCCGCGCCCGCCCGCCCCGTTACGCGTACGCGGCCCGCACGACGGCCGTGC‑TGGCGCAGCAGGAAG‑GGTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgc                                                                                       <  4:245282/136‑1 (MQ=255)
                                    gTCTTGAACACCATCGAGGAACCCCTCGCCCGCCCGGCCCGGGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgcg                                                                                      <  3:172248/136‑1 (MQ=255)
                                           aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                               <  1:107707/136‑1 (MQ=255)
                                           aGACCATCGAGGAAACCCTCGCCAGCCAGTCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGc                                                                               >  8:214344/1‑136 (MQ=255)
                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAg                                                                        >  3:143698/1‑136 (MQ=255)
                                                  cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAg                                                                        >  6:125719/1‑136 (MQ=255)
                                                    aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGcc                                                                      >  7:197974/1‑136 (MQ=255)
                                                       aaccccTCGCCCGCCAGGCCCGTGACGCGGAAGCGGCACCCACGAAGGCCCGGC‑GGGCGCAGCAGGAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                   <  4:297809/133‑1 (MQ=255)
                                                            cTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGc                                                              >  2:220449/1‑136 (MQ=255)
                                                            cTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGc                                                              >  2:227/1‑136 (MQ=255)
                                                                 ccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTg                                                         >  5:182212/1‑136 (MQ=255)
                                                                  cgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTg                                                         >  5:252094/1‑135 (MQ=255)
                                                                        gCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgc                                                  >  5:136256/1‑136 (MQ=255)
                                                                              gAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaa                                             >  2:162043/1‑135 (MQ=255)
                                                                                aGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAg                                          >  8:281571/1‑136 (MQ=255)
                                                                                     acgagGCACGCACGAA‑GCCCGGCGTGCCGATGCTGGAATCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCGGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                     <  3:150726/134‑1 (MQ=255)
                                                                                     aaGCGGCCCGCACGACGGCCCGGC‑TGGCGAAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                     <  3:90303/136‑1 (MQ=255)
                                                                                             cGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCAccc                             >  4:89267/1‑136 (MQ=255)
                                                                                             cGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCAccc                             >  5:136904/1‑136 (MQ=255)
                                                                                                     ggCCCTGC‑TGGCGCAGCAGTAAG‑GGGAAAAACGCCGCCAGCTGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAACGCCCGTACCACCCTCGGGCCg                     <  6:155811/136‑1 (MQ=255)
                                                                                                     ggCCCTGC‑TGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGcc                      >  2:21342/1‑135 (MQ=255)
                                                                                                           gc‑tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGtt               >  8:274380/1‑136 (MQ=255)
                                                                                                              tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCAGGCCGATGGTTTc             >  1:247136/1‑136 (MQ=255)
                                                                                                                      cagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATg     <  6:95197/136‑1 (MQ=255)
                                                                                                                         gAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgcg  >  3:4715/1‑136 (MQ=255)
                                                                                                                                |                                                                                                                                 
CAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGC‑TGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCG  >  ACB143/5760466‑5760722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑