Predicted mutation
evidence seq id position mutation freq annotation gene description
JC ACB143 2,142,770 (TCGATCTGGCTT)1→2 81.2% coding (807/3276 nt) rne ← Ribonuclease E

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 2142759 =7 (0.150)27 (0.620) 19/224 NT 81.2% coding (818/3276 nt) rne Ribonuclease E
?ACB143 = 2142770 6 (0.140)coding (807/3276 nt) rne Ribonuclease E

TACTGATCGACAGCATCGACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB143/2142897‑2142759
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccagatcgaAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGCGCGCGCCACCAAAGGCAGCGACATCGAAG  <  ACB143/2142770‑2142637
                                                                                                                                                                                                                                                                                 
TACTGATCGACAGCATCGACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGC                                                                                                                                                >  7:142636/1‑131
TACTGATCGACAGCATCGACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGC                                                                                                                                                >  6:65818/1‑131
        GACAGCATCGACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGC                                                                                                                                    >  7:46895/1‑135
             CATCGACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACTCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGC                                                                                                                                    >  3:98284/1‑130
             CATCGACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGC                                                                                                                                    >  7:233045/1‑130
              ATCGACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCG                                                                                                                             <  2:42057/136‑1
                  ACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGAC                                                                                                                         >  8:23582/1‑136
                  ACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGAGATGCCGCAGATAGCCAGCAAGGTCAACCTGTACGAAGACAGCGTACCGCGGTACAACCGCTTCCAGATAGAAAGCCAGAACGAAACCCCGATCGAGAC                                                                                                                         >  4:231963/1‑136
                             GAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGC                                                                                                              >  5:37965/1‑136
                                        CTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGAC                                                                                                   <  8:33942/136‑1
                                        CTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGAC                                                                                                   <  4:221332/136‑1
                                                GCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTC                                                                                           <  7:272336/136‑1
                                                                   CGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGAT                                                                         <  2:267205/135‑1
                                                                      CAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGAC                                                                     >  5:46727/1‑136
                                                                          AAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGA                                                                 <  8:238208/136‑1
                                                                           AGGGCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGAC                                                                <  8:285110/136‑1
                                                                            GGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCG                                                                                                             >  4:34756/1‑90
                                                                            GGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCG                                                                                                             <  3:34756/90‑1
                                                                                      TACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGG                                                     <  3:314179/136‑1
                                                                                         GAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGT                                                  <  1:79203/136‑1
                                                                                           AGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCG                                                <  3:240791/136‑1
                                                                                                   TACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACA                                         >  5:56219/1‑135
                                                                                                   TACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCCGCGGTTCCATCGTGATCGACCCGACCGAAGCCCTCGTGTGGATCGACA                                         >  1:136493/1‑135
                                                                                                   TACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAA                                                                                                                                       >  4:38054/1‑41
                                                                                                   TACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGAA                                                                                                                                       <  3:38054/41‑1
                                                                                                   TACCGCTGTTCAACAGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGTACGACCCGCCCGAAGCCCTGGTGTCGATCGCCAT                                        >  6:214404/1‑136
                                                                                                             CAACCGCTTCCAGATCGAAAGCCAGATCTAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGC                               <  5:83823/135‑1
                                                                                                             CAACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGC                               <  2:4361/135‑1
                                                                                                              AACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCATCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGCGC                             >  8:123189/1‑136
                                                                                                              AACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCCACTCGGCGC                             >  7:7806/1‑136
                                                                                                              AACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGCGC                             >  7:40336/1‑136
                                                                                                              AACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGCGC                             <  6:174328/136‑1
                                                                                                              AACCGCTTCCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGCGC                             <  4:71881/136‑1
                                                                                                                      CCAGATCGAAAGCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTAGATCGTGATCGACCCGACCGAAGCCCAGGTGTCGATAGACATCAACTCGGCGCGCGCCAC                      >  6:268211/1‑135
                                                                                                                                 GCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGCGCGCGCCACCAAAGGCAGCGA          >  3:132116/1‑136
                                                                                                                                 GCCAGATCGAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGCGCGCGCCACCAAAGGCAGCGA          >  2:237712/1‑136
                                                                                                                                         GAAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGCGCGCGCCACCAAAGGCAGCGACATCGAAG  <  5:65818/136‑1
                                                                                                                                                                                                                                                                                 
TACTGATCGACAGCATCGACGCCCAGGAAGAAGCGCTGACCTTCATCCGCCAGGTGATGCCGCAGTACGCCAGCAAGGTCAAGCTGTACGAAGACAGCGTACCGCTGTTCAACCGCTTCCAGATCGAAAGCCAGATCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  ACB143/2142897‑2142759
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccagatcgaAAGCCAGATCGAGACCGCCTTCCAGCGCGTGGTCGACCTGCCGTCCGGCGGTTCGATCGTGATCGACCCGACCGAAGCCCTGGTGTCGATCGACATCAACTCGGCGCGCGCCACCAAAGGCAGCGACATCGAAG  <  ACB143/2142770‑2142637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.