Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 924,333 T→G 12.2% D4780A (GAC→GCC)  CJ019_00881 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143924,3330TG12.2% 119.0 / 2.2 64D4780A (GAC→GCC) CJ019_00881hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (36/19);  new base G (4/5);  total (40/24)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGC  >  ACB143/924200‑924466
                                                                                                                                     |                                                                                                                                     
gCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcg                                                                                                                                     <  2:290392/136‑1 (MQ=255)
     cagcAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcgggcgg                                                                                                                                <  8:87705/133‑1 (MQ=255)
           ccAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGGTACGGACGATTAACGTGGCGCCGGCCTCGCCCAAGCCGCTGACCAAGCTTCCGTGGGGGGTGAACAACCCTGGGGGGAGGGGGGCGGGGGGGTTg                                                                                                                            >  2:241374/1‑134 (MQ=255)
                    tACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGGGTTGATCACCACTTGGGTGTGTGGGTCGGGCGGGGTGAAGTCCGGGGt                                                                                                                 <  6:56534/136‑1 (MQ=255)
                    tACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGt                                                                                                                 <  7:36464/136‑1 (MQ=255)
                    tACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCAATTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGt                                                                                                                 <  2:210907/136‑1 (MQ=255)
                       ccGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCCGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTg                                                                                                              >  2:199184/1‑136 (MQ=255)
                       ccGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTAACGTGGCGCCGGCCTCGCCCAGGCCGCTGCCCACGCTGCCGGGGGCGGTGATCACCACATGGGTGCGGGGGTCGGGCGGGGGGTAGTGCGGGGGTTg                                                                                                              >  2:162739/1‑136 (MQ=255)
                       ccGTGCCCAGTACGGTACCGGCCGCGGTACTGACGATTACCGTGGCGCCGGCCTCGCCCACGCCGCGGCCCACGCTGCCGTCGGCGGTGATCACCCCTGGGGGGGGTGGGGGGGGGGGGGGGTAGTGCGGGGGTTg                                                                                                              >  4:67996/1‑136 (MQ=255)
                        cGTGCCCAGTACGGTACCGGCCGCGGTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGc                                                                                                             <  7:236230/136‑1 (MQ=255)
                            cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCCGGCCGCTGACCCCGCTGCCGTCGGCGTTGATCACCACTTGGGGGGGGGGGTCGGGCGGGGTGGAGTCCGGGGTTTGCAggg                                                                                                         >  2:319177/1‑136 (MQ=255)
                            cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGCCCACGCTGCCGGCGGCGTTGATAACCACTTGGGTGAGTGGGTGGGGCGGGGTGAAGTGCGGGGGTTGGAggg                                                                                                         >  6:19052/1‑136 (MQ=255)
                            cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCCCGTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAggg                                                                                                         >  8:125386/1‑136 (MQ=255)
                            cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAggg                                                                                                         >  2:276473/1‑136 (MQ=255)
                            cccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGACGGCCTCGCCCAGGCCGCTGACCCCGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTGGGGGGGGGTGACGTCCGGGGTTTGCAggg                                                                                                         >  7:62775/1‑136 (MQ=255)
                                   cGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCGCGCCCAGGCCGCTGACCACGCTGCCGGCGGCGTTGATCACCACTTGGGTGAGTGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCAc                                                                                                  <  3:37922/136‑1 (MQ=255)
                                    ggTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCCCTGACCACGCTGCCGTCGGCGTTGATCACCACTGGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGGTTGCAGGGTAGCCACt                                                                                                 >  7:100381/1‑136 (MQ=255)
                                    ggTACCGGCCGCGTTACTGACGATTACCGGGGCGCCGGCCGCGCCCAGGCCGCTGACCCCGCTGCCGGCGGGGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACt                                                                                                 <  5:10026/136‑1 (MQ=255)
                                       aCCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGcc                                                                                              >  8:37959/1‑136 (MQ=255)
                                              gcgTTTCTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGGCGGCGGTGATCACCACGTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGGTTGCAGGGTTGCCACTGCCGACACGt                                                                                       >  1:238118/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGTGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGGTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGt                                                                                       >  3:158746/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCCGGCCGCTGACCACGCTGCCGTCGGGGGTGATAAACACATTGGGGAGTGGGTGGGGCGGGGTGAAGTGCGGGGGTTTGAGGGTTGCCACAGCCGCCACgg                                                                                       >  6:10026/1‑135 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTTCCGTCGGCGTTGATCAACACATGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTTGACGGTAGCCACTGCCGACCCGt                                                                                       >  6:291403/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTTATAACCACTTGGGTGAGTGGGTCGGTCGGGGGGAAGTACGGGGTTTTCAGGGTTGCCACTTGCGACACGt                                                                                       >  2:128562/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGt                                                                                       >  1:192700/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGt                                                                                       >  4:74399/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGt                                                                                       >  4:145872/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGt                                                                                       >  3:277612/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGt                                                                                       >  2:109056/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCCTTGATCACCAAATTGGTGAGTGGGGCTGGGGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCAGCCACGt                                                                                       >  3:199011/1‑136 (MQ=255)
                                              gcgTTACTGACGATTACCGTGGCGCCCGCCTCGCCCAGGCCGCTGACCACCCTGCCGTCGGCGTTGATAAACACTTTGGGGAGTGGGTCGGGCGGGGGGAAGGCCGGGGTTTTGAGGGTAGCCCCTGCCCACCCGt                                                                                       >  4:37922/1‑136 (MQ=255)
                                                 ttACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCAGCCGTCGGCGTTGATAACCACCTTGGTGAGTGGGTCGGGCGGGGGGGAGTGCGGGGGTTGGAGGGTAGCCCCTGCCGCCCCGTTgc                                                                                    >  2:116369/1‑136 (MQ=255)
                                                   aCTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCCCGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTAGGGCGGGGTGAAGTCCGGGGTTTTCAGGGTAGCCACTGCCGACACGTTgccg                                                                                  >  5:305270/1‑136 (MQ=255)
                                                   aCTGACGATTACCGTGGCGCCCGCCTCGCCCACGCCGCGGACCACCCAGCCGTCGGCGGTGATCACCCCCTGGGGGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCCCGGCCGACCCCTTgccg                                                                                  >  6:10476/1‑136 (MQ=255)
                                                       actaTTCCCGTGGCGCGGGCCTCGCCCAGGCCGCGGACCACGCTGCCGTCGGCGTTGATCCCCACTTGGGTGCGGGGGGCGGGCGGGGTTAAGGCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTgccggcc                                                                               <  7:283055/132‑1 (MQ=255)
                                                        ctaTTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTgccggccg                                                                              <  6:155193/133‑1 (MQ=255)
                                                             aCCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCgg                                                                        >  5:97597/1‑136 (MQ=255)
                                                             aCCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCgg                                                                        >  1:229544/1‑136 (MQ=255)
                                                                ggggCGCCGGCCGCGCCCAGGCCGGTCCCCACGCCGCCGTCGGCGGTGCTCCCCACTTGGGTGAGTGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCt                                                                     <  4:86727/134‑1 (MQ=255)
                                                                gTGGCGCCGGCCTCGCCCTGGCCGCTTACCCCGCTGCCGTCGGCGTTGATCACCACTTGTGTGAGGGGGGCGGGCGGGGTGAAGTCGGGGGTTTTCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCt                                                                     <  8:235825/136‑1 (MQ=255)
                                                                gTGGCGCCGGCCTCGCCCCGGCCGCGGCCCACGCTGCCGGCGGCGTTGATCCCCACTTGGGGGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCt                                                                     <  7:123629/136‑1 (MQ=255)
                                                                  ggCGCCGGCCGCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTgg                                                                   <  8:278055/136‑1 (MQ=255)
                                                                          ccTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCt                                                           <  7:18171/136‑1 (MQ=255)
                                                                            tCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCt                                                           >  3:319103/1‑134 (MQ=255)
                                                                               cccAGGCCGCTGACCACGCTGCCGTCGGCGTGGATCACCACTTGGGGGAGTGGGGCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGagca                                                      <  4:187851/136‑1 (MQ=255)
                                                                                    gCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTGGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCGGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCg                                                 <  7:3299/136‑1 (MQ=255)
                                                                                    cccGCGGCCCACGCTGCCGTCGGCGTTGATCCCCACTTGGGGGAGGGGGTCGGGCGGGGGGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCg                                                 <  7:217433/135‑1 (MQ=255)
                                                                                       gCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCg                                              >  4:79062/1‑136 (MQ=255)
                                                                                           aCCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACCCGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGc                                          >  2:146501/1‑136 (MQ=255)
                                                                                           aCCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGCGCAGCTCGGCGTTGc                                          >  6:109635/1‑136 (MQ=255)
                                                                                           aCCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGtt                                            <  4:204728/134‑1 (MQ=255)
                                                                                           aCCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGc                                          >  3:255671/1‑136 (MQ=255)
                                                                                           aCCACGCTGCCGTCGGCGTTGATCAACACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGc                                          >  7:153405/1‑136 (MQ=255)
                                                                                                  tGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGc                                   >  8:244469/1‑136 (MQ=255)
                                                                                                   gCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGcc                                  >  1:116405/1‑136 (MQ=255)
                                                                                                     cGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCgg                                <  7:28703/136‑1 (MQ=255)
                                                                                                          gCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCa                           <  8:172792/136‑1 (MQ=255)
                                                                                                                aTCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTg                      <  7:157571/135‑1 (MQ=255)
                                                                                                                 tCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGAt                    >  1:323811/1‑136 (MQ=255)
                                                                                                                             tGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTggg                                                                  >  3:280526/1‑78 (MQ=255)
                                                                                                                             tGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTggg                                                                  <  4:280526/78‑1 (MQ=255)
                                                                                                                             tGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGc         >  2:126492/1‑135 (MQ=255)
                                                                                                                                  gggTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTgg   >  5:275870/1‑136 (MQ=255)
                                                                                                                                   ggTCGGGGGGGTGGATGGCCGGGTTTTGCAGGGTAGCCACTGCCGAGACGTTGCGGGCGGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTAAGCGTGTTGGTGAAGTGGCCACTGGc  <  6:2999/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
GCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGC  >  ACB143/924200‑924466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑