Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,643,529 C→T 10.0% S196N (AGC→AAC)  CJ019_01543 ← putative TonB‑dependent receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,643,5290CT10.0% 117.2 / 4.9 40S196N (AGC→AAC) CJ019_01543putative TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base C (18/18);  new base T (2/2);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

TAGGCACCACCGATTGCGCCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCGGCACCGTGGATGCCCTCGATCGGCGAGTCCGGGATG  >  ACB143/1643394‑1643663
                                                                                                                                       |                                                                                                                                      
tAGGCACCACCGATTGCGCCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTggcggc                                                                                                                                        <  4:303715/136‑1 (MQ=255)
   gCACCACCGATTGCGCCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGt                                                                                                                                     <  2:302306/136‑1 (MQ=255)
        accGATTGCGCCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATTCGCAGGTCGTTGAACTGGCGGTTGTTTGCa                                                                                                                                >  4:65643/1‑136 (MQ=255)
        accGATTGCGCCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGTTGTTGGCa                                                                                                                                >  4:61184/1‑136 (MQ=255)
          cGATTGCGCCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATc                                                                                                                              >  7:203560/1‑136 (MQ=255)
          cGATTGCGCCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCACACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATc                                                                                                                              >  6:108941/1‑136 (MQ=255)
          cGATTGCGCCGCCGTCCTGGCGACCGTCGCTGGTTTACAGGCGGTGTTTGCGGCCGGGCTAGTCGGCATCGCGCCCCTTGGCGCTGCGAGCATAGCCCCGGATGCCGAGGGCGGTGACATGGCGGCGGTTTGCAt                                                                                                                               >  4:19869/1‑135 (MQ=255)
            aTTGCGCCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATc                                                                                                                              >  7:172308/1‑134 (MQ=255)
               gcgcCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGt                                                                                                                         >  4:189333/1‑136 (MQ=255)
                    gccgTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAgg                                                                                                                    >  7:88520/1‑136 (MQ=255)
                                 ccGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGt                                                                                                       <  5:90557/136‑1 (MQ=255)
                                 ccGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGt                                                                                                       <  3:88879/136‑1 (MQ=255)
                                     cGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGcc                                                                                                   >  8:70823/1‑136 (MQ=255)
                                     cGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAAATGGCGGCTGTTGGCATCCAGGTGCAGGGGGAAGGCGCCGtgccc                                                                                                   >  4:169782/1‑132 (MQ=255)
                                       cTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCgg                                                                                                 >  2:259685/1‑136 (MQ=255)
                                            ttCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTc                                                                                             <  2:96133/135‑1 (MQ=255)
                                            ttCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGAGGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTc                                                                                             <  6:196956/135‑1 (MQ=255)
                                                aGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCt                                                                                        <  6:233310/136‑1 (MQ=255)
                                                aGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCt                                                                                        <  7:169488/136‑1 (MQ=255)
                                                        tttGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGTTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGgc                                                                                 <  3:61184/135‑1 (MQ=255)
                                                        tttGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGGTGAACTGGCGGTTGTTGGCATCCAGGGGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGgc                                                                                 <  3:65643/135‑1 (MQ=255)
                                                                  ggCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCG‑CGGCCTCCAGCTTGCCGGCGCGGGGGCGAgt                                                                     >  2:134118/1‑136 (MQ=255)
                                                                                    aCCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCCGCCTCCAGCTTTCCGGCGCGGCTGCGAGTGGTGTATGCGCCGCCGt                                                    >  4:263119/1‑136 (MQ=255)
                                                                                    aCCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCCGCCGCCCCCTTGCCGGCGCGGGTGGGAGTGGTGGGGGCGccgccgg                                                    >  3:310481/1‑135 (MQ=255)
                                                                                    aCCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCCGCCCCACGCATGCCGGCGCGGCGGCGAGTGGTGGAGGCGccgccg                                                     >  4:25664/1‑135 (MQ=255)
                                                                                          gcgcTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACg                                                >  2:90410/1‑134 (MQ=255)
                                                                                             cTGCGTGCATAGCCCGGGATGCGCAGGGCGTTGAACTGGCGGCTGTTGGCAGACAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGtt                                           <  6:23955/136‑1 (MQ=255)
                                                                                                cGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCAc                                        <  7:65176/136‑1 (MQ=255)
                                                                                                cGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCGGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCAc                                        <  2:281643/136‑1 (MQ=255)
                                                                                                  tGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCTGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCg                                      <  3:19869/136‑1 (MQ=255)
                                                                                                  ggCATAGCCCGGGATGCGCAGGTCGTGGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCg                                      <  1:190341/135‑1 (MQ=255)
                                                                                                         cccGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCTGCCTCCAGCTTGCCGGCGCTGCTGCTAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCGGCACCGt                               <  5:102577/136‑1 (MQ=255)
                                                                                                         cccGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCGGCACCGt                               <  1:99784/136‑1 (MQ=255)
                                                                                                         cccGGGATGCGCAGGTCGTTGAACTGGCGGCTGGTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCGGCACCGt                               <  8:220655/136‑1 (MQ=255)
                                                                                                                tGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCCCCCGCCAGCAGCATGCCGGCGCAGCGGCGAGTGGTGTAGGCGCCCCCGGAACGCAGTTAACCCGCCCCGTCGAAGcc                        >  4:230372/1‑136 (MQ=255)
                                                                                                                     aGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCGGCCCCGTGGATTCCCTCg                    >  6:150282/1‑135 (MQ=255)
                                                                                                                       gTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCGGCACCGTGGATGCCCTCGATc                 <  8:301951/136‑1 (MQ=255)
                                                                                                                          gTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCCCCGGCCTCCCGCTTGCCGGCGCTGCTGCGAGTGGTGTAGGCGCCGCCGGAACGCCCTTCCCCCGCCCCGTGGATGCCCGCGGTCGGc              >  4:163276/1‑136 (MQ=255)
                                                                                                                             gAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCGGCACCGTGGATGCCCTCGATCGGCGa            <  8:187947/135‑1 (MQ=255)
                                                                                                                              aaCTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGGGTCGGCGCCGCCGTAACGCAGTTCACCGGCACCGTGGATGCCCTCGATCGGCGAGt          <  3:221032/136‑1 (MQ=255)
                                                                                                                               aCTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTACAGCTTTCCGGCGCTTCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTAACCGGCACCGTTGATTCCCACGATAGGCGAGta         >  8:235584/1‑135 (MQ=255)
                                                                                                                                      gcTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCGGCACCGTGGATGCCCTCGATCGGCGAGTCCGGGATg  <  2:216788/136‑1 (MQ=255)
                                                                                                                                      gcTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCCGCGCCGCCGTAACGCACTTACCCCGCACCCTGGATTCCCACGATCGGCGAGTCCCGGATg  >  8:209103/1‑136 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TAGGCACCACCGATTGCGCCGCCGTCCTGGCGACCGTCGCTGTTTTCCAGGCGGTGTTTGCTGCCGGGCTCGTCGGCATCGCGCACCTTGGCGCTGCGTGCATAGCCCGGGATGCGCAGGTCGTTGAACTGGCGGCTGTTGGCATCCAGGTGCAGGGCGAAGGCGCCGTCGCCGGCCTCCAGCTTGCCGGCGCTGCTGCGAGTGGTGTCGGCGCCGCCGTAACGCAGTTCACCGGCACCGTGGATGCCCTCGATCGGCGAGTCCGGGATG  >  ACB143/1643394‑1643663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑