Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,598,770 C→T 11.8% T80T (ACC→ACT)  CJ019_02459 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,598,7700CT11.8% 112.7 / 7.6 43T80T (ACC→ACT) CJ019_02459hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (19/18);  new base T (3/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01

ACCGTCTCTGAGGGACTGATGAAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCACCGTGGCGGACTACACCTTCA  >  ACB143/2598639‑2598903
                                                                                                                                   |                                                                                                                                     
aCCGTCTCTGAGGGACTGATGAAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACTAAcg                                                                                                                                   <  5:156905/136‑1 (MQ=255)
aCCGTCTCTGAGGGACTGATGAAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAAcg                                                                                                                                   <  3:270333/136‑1 (MQ=255)
        tGAGGGACTGATGAAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGa                                                                                                                              <  7:199188/133‑1 (MQ=255)
               cTGATGAAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTAt                                                                                                                    <  5:47915/136‑1 (MQ=255)
                 gatgaAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATAACCAACCGCGGACTCCGTGTAttt                                                                                                                  >  7:111487/1‑136 (MQ=255)
                  atgaAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACTAACGGCGGACTCCGTGTAttt                                                                                                                  <  7:38151/135‑1 (MQ=255)
                  atgaAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTAttt                                                                                                                  <  5:58532/135‑1 (MQ=255)
                  atgaAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTAttt                                                                                                                  <  3:284166/135‑1 (MQ=255)
                             cccAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACTAACGGCGGACTCCGTGTATTTTCCGTATACgg                                                                                                       >  6:197792/1‑135 (MQ=255)
                             cccAACCACCCACAAGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCAAACAATCAAACGCGATGCCAGCGAGCGCTACCAAGAGGCGATCACCAACGGCGGACACCGTGTACTTGCCCTAGACgg                                                                                                       >  1:87689/1‑135 (MQ=255)
                                aaCCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACt                                                                                                   <  2:208356/136‑1 (MQ=255)
                                     cccaccTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAgcg                                                                                              <  3:121678/136‑1 (MQ=255)
                                        accTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGATCGCTACCAAGTGGCGATCACCAACCGCGGACTCCGGGTATTTTCCGTAGACGGCAATGAGCGCAc                                                                                           >  4:182060/1‑136 (MQ=255)
                                        accTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCCCCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCAc                                                                                           >  2:212158/1‑136 (MQ=255)
                                           tGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCCCCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGCGCGCACGgt                                                                                        >  2:126841/1‑136 (MQ=255)
                                           tGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGgt                                                                                        >  3:247493/1‑136 (MQ=255)
                                           tGGCCCGAGTGACTGCTTCCACATTGGAGTCGGCATTCGTCCACACAATCAACCGCGATGCCAGCGAGCGCTACCAACTGGCGATCACAAACGGCGGACACCTTGTATTTGCCGTAGACGTCACTGAGCGCACGgt                                                                                        >  6:267339/1‑136 (MQ=255)
                                             gCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCAATAACGGCGGACTCCGTGTATTTGCCGTAGACCGCACTGAGCGCACGgtg                                                                                       >  2:10267/1‑135 (MQ=255)
                                                cGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCtt                                                                                   >  5:266421/1‑136 (MQ=255)
                                                   gTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTcc                                                                                >  2:25092/1‑136 (MQ=255)
                                                    tGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTccc                                                                               <  5:69253/136‑1 (MQ=255)
                                                     gACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCg                                                                              >  8:68421/1‑136 (MQ=255)
                                                          cTTCCCCATTGGAGTCGGCATTAGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACTGCGGACTCCGTGTATTTGCCGTAGACGGCACTGATCGCACGGTGTATTTACCGGATgg                                                                         >  5:334/1‑136 (MQ=255)
                                                                 aTTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGc                                                                  <  5:262985/136‑1 (MQ=255)
                                                                 aTTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGc                                                                  <  2:34436/136‑1 (MQ=255)
                                                                             aTTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACTAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGc                                                       >  1:93630/1‑135 (MQ=255)
                                                                             aTTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTTCCGTAGACGGCACTGAGCGCACGGTGTCGTTCCCGGATGGAACCGGCTACCTGGCCGc                                                       >  7:108766/1‑135 (MQ=255)
                                                                                    caccCCATCACCCGCGATGCCAGCGAGCGCTACCAAGTGGCGAGCACCACCGGCGGACTCCGTGTTTTTGCCGTAGACGGCACTGAGCGCAGGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGAcc                                               <  6:16321/136‑1 (MQ=255)
                                                                                              aCCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGa                                     >  2:132436/1‑136 (MQ=255)
                                                                                              aCCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGa                                     >  8:270233/1‑136 (MQ=255)
                                                                                                      gCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACcg                             >  7:44870/1‑136 (MQ=255)
                                                                                                       ccAGCGAGCGCTACCACGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGTATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACcgc                            <  4:115204/136‑1 (MQ=255)
                                                                                                       ccAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACcgc                            >  8:7900/1‑136 (MQ=255)
                                                                                                            gagcgCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCa                       >  1:242252/1‑136 (MQ=255)
                                                                                                              gcgcTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCAcc                     <  7:193460/136‑1 (MQ=255)
                                                                                                               cgcTACCACGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCACCg                    <  3:177287/136‑1 (MQ=255)
                                                                                                               cgcTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCACCg                    <  8:124769/136‑1 (MQ=255)
                                                                                                                    ccAAGTGGCGATCACCAACGGCGGACTCCGGGTATTGGCCGTAGACGGCACTGAGCGCACGGGGTCTTTCCCGGAGGGAACGGGCTACGTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCACCGTGGCg               <  8:108766/136‑1 (MQ=255)
                                                                                                                      aaGTGGCTATCACCAAGGGCGGACTCCGGGTATTTGCCGTAGACGGCACTGAGCTCACGGTGGCTTTCCCGGATGGAACCGGCTACGTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCACCGTGGCGGa             <  6:334/136‑1 (MQ=255)
                                                                                                                           gCGATCACCAAGGGCGGACTCGGTGTATTTGCCGTAGACGGCAGTGAGCGCACGGTGTCTTTCCCGTATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCACCGTGGCGGACTaca        <  7:131115/136‑1 (MQ=255)
                                                                                                                               tCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCACGCGACTTCACCGCGATCACCGTGGCGGACTACCCCtt    >  8:268523/1‑136 (MQ=255)
                                                                                                                                 aCCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCACCGTGGCGGACTACACCTTCa  >  4:204756/1‑136 (MQ=255)
                                                                                                                                 aCCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCACCGTGGCGGACTACACCTTCa  >  5:102338/1‑136 (MQ=255)
                                                                                                                                   |                                                                                                                                     
ACCGTCTCTGAGGGACTGATGAAGCGCCCCCCAACCACCCACCTGGCCCGAGTGACTGCTTCCCCATTGGAGTCGGCATTCGTCCACACCATCAACCGCGATGCCAGCGAGCGCTACCAAGTGGCGATCACCAACGGCGGACTCCGTGTATTTGCCGTAGACGGCACTGAGCGCACGGTGTCTTTCCCGGATGGAACCGGCTACCTGGCCGCAAGCGACCCGGCAAGCGACTTCACCGCGATCACCGTGGCGGACTACACCTTCA  >  ACB143/2598639‑2598903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑