Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,779,225 A→G 10.8% S162P (TCG→CCG)  alx_1 ← Putative membrane‑bound redox modulator Alx

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,779,2250AG10.8% 129.3 / 7.4 47S162P (TCG→CCG) alx_1Putative membrane‑bound redox modulator Alx
Reads supporting (aligned to +/- strand):  ref base A (20/21);  new base G (3/2);  total (24/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01

CTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCAGGATGCCCCAGAA  >  ACB143/2779094‑2779360
                                                                                                                                   |                                                                                                                                       
cTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGCGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCTCGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAgc                                                                                                                                     >  8:148902/1‑136 (MQ=255)
cTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGGCAgc                                                                                                                                     >  5:147910/1‑136 (MQ=255)
cTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGCCAgc                                                                                                                                     >  3:285915/1‑136 (MQ=255)
cTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAgc                                                                                                                                     >  3:75167/1‑136 (MQ=255)
cTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAgc                                                                                                                                     >  6:229386/1‑136 (MQ=255)
cTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAgc                                                                                                                                     >  7:169070/1‑136 (MQ=255)
cTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAgc                                                                                                                                     >  3:160834/1‑136 (MQ=255)
     aaagaccggccggGGTATGGCGTAATAAGAAGTTATAGAAATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCTGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCAt                                                                                                                                >  4:316099/6‑136 (MQ=255)
          ccggccggGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTGACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCa                                                                                                                           <  3:269401/136‑1 (MQ=255)
          ccggccggGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCTCGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCa                                                                                                                           >  5:290801/1‑136 (MQ=255)
            ggccggGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCAcc                                                                                                                         >  3:172661/1‑136 (MQ=255)
            ggccggGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCAcc                                                                                                                         >  8:298261/1‑136 (MQ=255)
               cggGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCg                                                                                                                      <  7:119069/136‑1 (MQ=255)
               cggGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCg                                                                                                                      <  5:38332/136‑1 (MQ=255)
                           tACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGGCAGCGCCATTTTCACCCCGGTAAAcagcag                                                                                                           >  7:41119/1‑135 (MQ=255)
                               aagaagTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGGCAGCGCCATTTTCACCCCGGTAAACAGCAGGAAgg                                                                                                      >  5:76211/1‑136 (MQ=255)
                                     tGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGa                                                                                                <  7:298261/136‑1 (MQ=255)
                                                gATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACTGCAGGAAGGCACCGAAGATGTAAAg                                                                                      <  5:113084/135‑1 (MQ=255)
                                                  tCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCAc                                                                                   >  3:22293/1‑136 (MQ=255)
                                                      gTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGGCAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAg                                                                               <  6:76826/136‑1 (MQ=255)
                                                      gTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAg                                                                               <  1:96718/136‑1 (MQ=255)
                                                       tCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAgg                                                                              <  6:184122/136‑1 (MQ=255)
                                                        cGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAgg                                                                              >  4:203417/1‑135 (MQ=255)
                                                         ggTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGc                                                                             >  5:259375/1‑135 (MQ=255)
                                                         ggTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGc                                                                             >  1:159130/1‑135 (MQ=255)
                                                              cccGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGtt                                                                       >  1:159162/1‑136 (MQ=255)
                                                                            cgcACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAgcgccg                                                         >  5:113372/1‑136 (MQ=255)
                                                                                 gAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGccc                                                    <  3:133078/136‑1 (MQ=255)
                                                                                   aCTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCAc                                                  >  5:215681/1‑136 (MQ=255)
                                                                                   aCTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCAc                                                  >  1:267627/1‑136 (MQ=255)
                                                                                           aTCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCa                                          <  4:202464/136‑1 (MQ=255)
                                                                                           aTCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCa                                          <  4:172661/136‑1 (MQ=255)
                                                                                                gttgttGGCCAGGTCTGGGCGACTGTCTTCCTTCGGCAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCatgatg                                     <  8:41119/136‑1 (MQ=255)
                                                                                                          aGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGa                           <  7:52978/136‑1 (MQ=255)
                                                                                                            ggcTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAac                         <  8:33137/134‑1 (MQ=255)
                                                                                                            gTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAac                         <  1:7368/136‑1 (MQ=255)
                                                                                                             tCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAaca                        <  4:160834/136‑1 (MQ=255)
                                                                                                               tGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGGCGATCATGATGGCCCGCAGGAAcacc                      >  2:301639/1‑136 (MQ=255)
                                                                                                               tGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAAcacc                      >  3:63937/1‑136 (MQ=255)
                                                                                                                      cTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAATGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCa               <  1:282179/136‑1 (MQ=255)
                                                                                                                      cTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCa               <  3:7622/136‑1 (MQ=255)
                                                                                                                      cTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCa               <  8:15217/136‑1 (MQ=255)
                                                                                                                      cTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCa               <  8:164133/136‑1 (MQ=255)
                                                                                                                      cTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCa               <  4:298508/136‑1 (MQ=255)
                                                                                                                      cTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCAACGCCGATCATTATGGCCCGCAGGAACACCACGCCCa               <  5:286520/136‑1 (MQ=255)
                                                                                                                         tCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCAGGa            >  5:156431/1‑136 (MQ=255)
                                                                                                                             ccttcGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCAGGATg          <  1:217424/134‑1 (MQ=255)
                                                                                                                                   aCAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCAGGATGCCCcagaa  >  2:288610/1‑136 (MQ=255)
                                                                                                                                   |                                                                                                                                       
CTTGCTGTGCCCGGCCGGGGTATGGCGTACGAAGAAGTGCGAGCCATGGATCTGGTCGGTAACCCGCATGTGCCGCCGCACGAACTTGAGGATCGGGTTGTTGGCCAGGTCTGGGCGACTGTCTTCCTTCGACAGCGCCATTTTCACCCCGGTAAACAGCAGGAAGGCACCGAAGATGTAAAGCACCCAGGCAAAGTTCTGTACCAGCGCCGCGCCCACGCCGATCATGATGGCCCGCAGGAACACCACGCCCAGGATGCCCCAGAA  >  ACB143/2779094‑2779360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑