Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 3,185,620 G→A 13.9% P302L (CCG→CTG)  prr_2 ← Gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,185,6200GA13.9% 113.3 / 9.0 43P302L (CCG→CTG) prr_2Gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (17/20);  new base A (3/3);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGGTTGACGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTCGGCATCGTCGAAGATCAG  >  ACB143/3185489‑3185741
                                                                                                                                   |                                                                                                                         
cccGGTTGACGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCggg                                                                                                                         >  1:78648/1‑134 (MQ=255)
     ttGACGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCg                                                                                                                  >  2:96185/1‑136 (MQ=255)
       gACGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGc                                                                                                                 <  2:170505/135‑1 (MQ=255)
        aCGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCt                                                                                                               >  3:262754/1‑136 (MQ=255)
        aCGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCt                                                                                                               <  4:122868/136‑1 (MQ=255)
        aCGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCt                                                                                                               <  2:91967/136‑1 (MQ=255)
        aCGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTGGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTTCGAACGTACTCCGGGTAGAGCGCCt                                                                                                               <  4:122866/136‑1 (MQ=255)
         cGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCAGGTAGACCGCCtt                                                                                                              <  8:1277/136‑1 (MQ=255)
                          tGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACTAACTCCAGGTAGACCGCCTTCTGCACATAGAGGCGac                                                                                             <  3:72868/136‑1 (MQ=255)
                           gCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGaca                                                                                            >  5:222881/1‑136 (MQ=255)
                           gCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCAGGTAGACCGCCTTCTGCACATAGAGGCGaca                                                                                            >  2:20748/1‑136 (MQ=255)
                             aGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACAcg                                                                                          >  3:63827/1‑136 (MQ=255)
                             aGGTGCTCTTGAGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCAGGTAGACCGCCTTCTGCACATAGAGGCGACAcg                                                                                          >  2:162751/1‑136 (MQ=255)
                               gTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGATTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACAcg                                                                                          <  4:314380/134‑1 (MQ=255)
                                  ctctTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGCCCCGGCTGCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGcc                                                                                     <  5:86450/136‑1 (MQ=255)
                                  ctctTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGcc                                                                                     <  2:78648/136‑1 (MQ=255)
                                  ctctTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGcc                                                                                     <  6:110090/136‑1 (MQ=255)
                                  ctctTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGcc                                                                                     <  7:11813/136‑1 (MQ=255)
                                  ctctTGGGTGATCAGCGGGCCAATTTCGGTTGCCGGGTCATCCTCCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCAGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGcc                                                                                     <  2:51843/136‑1 (MQ=255)
                                  cgctgggggGAGCAGCGGCCCCATTGGGGTGGCCGGGTCATCCTGAAACCCACATTTGATGCTGGACACGGCTCCGCCAATCTTGCTAACCACCTCCGGGTAGCCCGCCTTCTGCACATTCAGGCGACACGCGGcc                                                                                     <  8:115369/131‑1 (MQ=255)
                                     ttGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCt                                                                                                               <  3:103509/107‑1 (MQ=255)
                                     ttGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCt                                                                                                               >  4:103509/1‑107 (MQ=255)
                                     ttGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTa                                                                                  >  1:227855/1‑136 (MQ=255)
                                       gggTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTa                                                                                  >  1:173347/1‑134 (MQ=255)
                                       gggTGATCAGCGGGCCCATTTCGCTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTAGCGAACGAACTCCGGCTAGTCCGCCTTCTGCACATAGACGCGACACGCCGCCGTACa                                                                                >  4:248532/1‑136 (MQ=255)
                                               aGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTgga                                                                        >  1:74737/1‑135 (MQ=255)
                                               aGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGc                                                                        >  8:26780/1‑136 (MQ=255)
                                               aGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGc                                                                        >  6:217148/1‑136 (MQ=255)
                                               aGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGc                                                                        >  2:134674/1‑136 (MQ=255)
                                                gCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACCCGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGcc                                                                       <  3:122913/136‑1 (MQ=255)
                                                              gTTGCGGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGGTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGCGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGc                                                         <  8:35405/136‑1 (MQ=255)
                                                                      gTCATCCTGCAACCCACATTTGATGCGGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGgcg                                                 <  2:32363/136‑1 (MQ=255)
                                                                                  cccACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTc                                      >  2:143044/1‑135 (MQ=255)
                                                                                       atatGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTACTGGCCGGCGATGAAAAAGCCGAAGGCGCGAATGCCCTCGAcggc                                >  6:71174/4‑136 (MQ=255)
                                                                                               cTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGGAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGa                        >  1:230034/1‑136 (MQ=255)
                                                                                                   acacGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTc                    <  7:283404/136‑1 (MQ=255)
                                                                                                    cacGGCTTCGCCAAGCTTGCGAACGAACTCCAGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGAGCGAATGCCCTCGACGGCGGCGTCGATGTCg                   >  8:126350/1‑136 (MQ=255)
                                                                                                     acGGCTTCGCCAAGCTTGCGAACGATCTGCGGGTTGACGGCCTTCTGCTGATAGAGGCTACTCGCGGCCGTTCAGTCCTGGCGGGCGTTGTATAAGCGGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTCgg                  <  7:254452/136‑1 (MQ=255)
                                                                                                          ttCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTCGGCAtcg             >  7:633/1‑136 (MQ=255)
                                                                                                            cGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGCGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTCGGCAtcg             <  5:149429/134‑1 (MQ=255)
                                                                                                            cGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTCGGCAtcg             <  5:82287/134‑1 (MQ=255)
                                                                                                               cAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTCGGCAtcgtcg          >  7:48919/1‑134 (MQ=255)
                                                                                                               cAAGCTTGCCAACGACCTCCGGGTAGACCGCCTTCTGCACATCGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTCGGCATCGTCGaa        <  5:72389/136‑1 (MQ=255)
                                                                                                                      gCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTCGGCATCGTCGAAGATCAg  <  2:173347/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                         
CCCGGTTGACGAAGCCCTCGACACGCTGCAGGTGCTCTTGGGTGATCAGCGGGCCCATTTCGGTTGCCGGGTCATCCTGCAACCCACATTTGATGCTGGACACGGCTTCGCCAAGCTTGCGAACGAACTCCGGGTAGACCGCCTTCTGCACATAGAGGCGACACGCGGCCGTACAGTCCTGGCCGGCGTTGTAAAAGCCGAAGGCGCGAATGCCCTCGACGGCGGCGTCGATGTCGGCATCGTCGAAGATCAG  >  ACB143/3185489‑3185741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑