Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,156,467 C→T 5.9% R203H (CGT→CAT)  pssA ← CDP‑diacylglycerol‑‑serineO‑phosphatidyltransfer a se

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,156,4670CT 5.9% 234.6 / 3.3 68R203H (CGT→CAT) pssACDP‑diacylglycerol‑‑serineO‑phosphatidyltransfer a se
Reads supporting (aligned to +/- strand):  ref base C (40/24);  new base T (2/2);  total (42/26)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

AAGGCGCACAGCGCCCGGTTCAGTGGATTGCCACGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGCCGGTGACTG  >  ACB143/4156341‑4156591
                                                                                                                              |                                                                                                                            
aaGGCGCACAGCGCCCGGTTCAGTGGATTGCCACGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGt                                                                                                                     >  4:251547/1‑136 (MQ=255)
      cacaGCGCCCGGTTCAGTGGATTGCCACGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAACCGCCGAATGTCACcgc                                                                                                               >  8:162326/1‑136 (MQ=255)
        caGCGCCCGGTTCAGTGGATTGCCACGGCCCACCCCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCCGCCGTGCGCGCAAACGCCGAATGTCACCgcgc                                                                                                             >  1:147176/1‑136 (MQ=255)
           cgcCCGGTTCAGTGGATTGCCACGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCaaa                                                                                                          <  7:83753/136‑1 (MQ=255)
           cgcCCGGTTCAGTGGATTGCCACGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCaaa                                                                                                          <  8:100630/136‑1 (MQ=255)
                          aTTGCCACGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGt                                                                                           >  1:288/1‑136 (MQ=255)
                               cACGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGgcgc                                                                                      >  1:162295/1‑136 (MQ=255)
                               cACGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGgcgc                                                                                      >  3:156143/1‑136 (MQ=255)
                                aCGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGcc                                                                                     <  4:178490/136‑1 (MQ=255)
                                aCGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGcc                                                                                     <  4:22323/136‑1 (MQ=255)
                                    cccACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCgg                                                                                   >  8:183318/1‑134 (MQ=255)
                                      cacaCCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGTGTAGAAGGTGGCGCCGGCAgg                                                                               >  3:162976/1‑136 (MQ=255)
                                           ccAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAg                                                                          <  7:199805/136‑1 (MQ=255)
                                           ccAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAg                                                                          <  6:309673/136‑1 (MQ=255)
                                           ccAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAg                                                                          <  5:91707/136‑1 (MQ=255)
                                                 gcGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCCGCCGTGCGCGCACCCGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCgg                                                                    >  1:262000/1‑136 (MQ=255)
                                                 gcGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCgg                                                                    >  1:68926/1‑136 (MQ=255)
                                                 gcGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCgg                                                                    >  1:34732/1‑136 (MQ=255)
                                                 gcGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCgg                                                                    >  5:240276/1‑136 (MQ=255)
                                                   gggATCACCTGCAGGCCCTGCCCCGTGACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGgtg                                                                  <  8:136606/136‑1 (MQ=255)
                                                     gATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGtgtg                                                                >  3:295154/1‑136 (MQ=255)
                                                        cACCTTCAGGCCCGTCCCCGTCACGCTGCCCGGTACCTCGTTGTCCATCCTCCGCAGCCGTGCGCGCAAACGCCGACTGTCAACGCGCAAACTGCGGTTAGAATGTGGCGCCGGCAGTTCGAGGCGCGGTGTGGCg                                                             <  2:246637/136‑1 (MQ=255)
                                                         aCCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCg                                                             <  2:162295/135‑1 (MQ=255)
                                                          ccTGCAGGCCCTCCCCCGACACGCACGCCGCTACCACGAAGGCCATGCGCCGCAGCCGTGCGCGCACACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCgg                                                            >  2:106600/1‑135 (MQ=255)
                                                              cAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTCGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTAt                                                         <  4:232501/134‑1 (MQ=255)
                                                                  cccTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTgca                                                   <  3:315886/136‑1 (MQ=255)
                                                                      gCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCTCAAAATGCGGGTAGAAGGTGGCTCCGTCAGGTCGAGGCGAGGTGTGGCGGTATGGTgcagca                                                >  8:152550/1‑135 (MQ=255)
                                                                       ccccGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCaaa                                              >  8:58412/1‑136 (MQ=255)
                                                                        cccGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAAc                                             >  7:189649/1‑136 (MQ=255)
                                                                        cccGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAAc                                             >  5:271064/1‑136 (MQ=255)
                                                                        cccGTCACGCTGGCCGGTACATCGTAGGACATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAAc                                             >  5:217193/1‑136 (MQ=255)
                                                                              aCGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGaa                                       >  3:16534/1‑136 (MQ=255)
                                                                              aCGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGaa                                       >  1:167018/1‑136 (MQ=255)
                                                                               cGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAAc                                      >  8:55181/1‑136 (MQ=255)
                                                                                gCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAAcc                                     <  6:219242/136‑1 (MQ=255)
                                                                                    gCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAg                                   <  8:24587/134‑1 (MQ=255)
                                                                                    gCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGc                                 >  1:91937/1‑136 (MQ=255)
                                                                                       ggTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGa                              <  2:167018/136‑1 (MQ=255)
                                                                                            cTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCCCCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATg                         <  8:55967/136‑1 (MQ=255)
                                                                                            cTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAAcc                                     >  2:94988/1‑124 (MQ=255)
                                                                                            cTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAAcc                                     <  1:94988/124‑1 (MQ=255)
                                                                                            cTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATg                         <  3:160547/136‑1 (MQ=255)
                                                                                            cTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATg                         <  8:251912/136‑1 (MQ=255)
                                                                                                 aGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAg                                                                          >  1:97032/1‑82 (MQ=255)
                                                                                                 aGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAg                                                                          <  2:97032/82‑1 (MQ=255)
                                                                                                 aGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCAt                     >  1:32558/1‑135 (MQ=255)
                                                                                                 aGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCAt                     >  3:259961/1‑135 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  8:243213/1‑136 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  4:123475/1‑136 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  8:26842/1‑136 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  7:97481/1‑136 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  4:177857/1‑136 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  3:255180/1‑136 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  4:244178/1‑136 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  2:58069/1‑136 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  4:93474/1‑136 (MQ=255)
                                                                                                          gccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGc           >  6:312163/1‑136 (MQ=255)
                                                                                                           ccgcAGCCGTGCGCGCAAATGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAg                                                                          <  6:14200/72‑1 (MQ=255)
                                                                                                           ccgcAGCCGTGCGCGCAAATGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAg                                                                          >  5:10442/1‑72 (MQ=255)
                                                                                                           ccgcAGCCGTGCGCGCAAATGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAg                                                                          >  5:14200/1‑72 (MQ=255)
                                                                                                           ccgcAGCCGTGCGCGCAAATGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAg                                                                          <  6:10442/72‑1 (MQ=255)
                                                                                                           ccgcAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGcc          >  2:242993/1‑136 (MQ=255)
                                                                                                                gCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGCCGGTGa     <  6:216343/136‑1 (MQ=255)
                                                                                                                gCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGCCGGTGa     <  2:25265/136‑1 (MQ=255)
                                                                                                                 ccGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGCCGGTGAc    >  6:233362/1‑136 (MQ=255)
                                                                                                                 ccGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGGGTGGCGGTATGGTGCAGCAATCGCTGCACCAGGCCGACCATGGCATCAGCCAGTGCCGGTGAc    >  2:96482/1‑136 (MQ=255)
                                                                                                                   gTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGCCGGTGACTg  <  7:6074/136‑1 (MQ=255)
                                                                                                                   gTGCGCGCAAACGCCGAATGGCACCGCGCAAACTGCGGGTAGAAGGCGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCACCAAACGCTGAACCAGGCCGACAATGGCATCAGCCAGTGCCGGCGACtt  <  6:151147/136‑2 (MQ=255)
                                                                                                                              |                                                                                                                            
AAGGCGCACAGCGCCCGGTTCAGTGGATTGCCACGGCCCACACCCAGCAGCGGGATCACCTGCAGGCCCTGCCCCGTCACGCTGGCCGGTACCTCGTAGGCCATGCGCCGCAGCCGTGCGCGCAAACGCCGAATGTCACCGCGCAAACTGCGGGTAGAAGGTGGCGCCGGCAGGTCGAGGCGCGGTGTGGCGGTATGGTGCAGCAAACGCTGAACCAGGCCGACCATGGCATCAGCCAGTGCCGGTGACTG  >  ACB143/4156341‑4156591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑