Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,198,609 G→A 13.0% T932I (ACC→ATC)  CJ019_03944 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,198,6090GA13.0% 129.7 / 7.9 46T932I (ACC→ATC) CJ019_03944hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/17);  new base A (2/4);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 3.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

ACCCATGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTGCCAATGAGGTGACCATTCCGCTTG  >  ACB143/4198477‑4198742
                                                                                                                                    |                                                                                                                                     
aCCCATGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGgtgt                                                                                                                                    <  8:60556/136‑1 (MQ=255)
aCCCATGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGgtgt                                                                                                                                    <  5:261689/136‑1 (MQ=255)
aCCCATGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGgtgt                                                                                                                                    <  7:131593/136‑1 (MQ=255)
   cATGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACt                                                                                                                                 <  3:250543/136‑1 (MQ=255)
    aTGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTc                                                                                                                                >  6:105317/1‑136 (MQ=255)
    aTGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTc                                                                                                                                >  3:207464/1‑136 (MQ=255)
    aTGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTc                                                                                                                                >  2:284988/1‑136 (MQ=255)
    aTGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTc                                                                                                                                >  2:157337/1‑136 (MQ=255)
    aTGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTc                                                                                                                                >  1:94174/1‑136 (MQ=255)
        acaGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGt                                                                                                                             >  5:298321/1‑135 (MQ=255)
        acaGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGt                                                                                                                             >  3:315335/1‑135 (MQ=255)
               tCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTgg                                                                                                                       >  4:79915/1‑134 (MQ=255)
                  cTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGcc                                                                                                                  >  5:128838/1‑136 (MQ=255)
                  cTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGcc                                                                                                                  >  5:50406/1‑136 (MQ=255)
                  cTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGcc                                                                                                                  >  3:219645/1‑136 (MQ=255)
                   tCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGccc                                                                                                                 >  8:218478/1‑136 (MQ=255)
                   tCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGcc                                                                                                                  >  1:262918/1‑135 (MQ=255)
                      gCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGc                                                                                                              <  7:52149/136‑1 (MQ=255)
                         gTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCCGCCaa                                                                                                           >  5:41701/1‑136 (MQ=255)
                         gTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCaa                                                                                                           >  4:229459/1‑136 (MQ=255)
                          ttGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAAc                                                                                                          <  7:253937/136‑1 (MQ=255)
                                    gAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAAcc                                                                                                <  5:165669/136‑1 (MQ=255)
                                    gAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAAcc                                                                                                <  7:245921/136‑1 (MQ=255)
                                    gAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAAcc                                                                                                <  2:178062/136‑1 (MQ=255)
                                    gAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGATGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAAcc                                                                                                >  2:176460/1‑136 (MQ=255)
                                    gAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGATGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAAcc                                                                                                >  7:78754/1‑136 (MQ=255)
                                            aTCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAgcc                                                                                        <  3:148112/136‑1 (MQ=255)
                                               aGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGAACAgccgcc                                                                                     >  6:177892/1‑136 (MQ=255)
                                                  ccGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGccgcccgc                                                                                  >  5:39142/1‑136 (MQ=255)
                                                     tAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGATGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGc                                                                               <  1:243424/136‑1 (MQ=255)
                                                       gctcgAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGGGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGcac                                                                             <  7:81775/133‑1 (MQ=255)
                                                               cgcCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCa                                                                     <  8:180429/136‑1 (MQ=255)
                                                                     tCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAg                                                               >  6:205480/1‑136 (MQ=255)
                                                                         gTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAgg                                                              <  1:157337/133‑1 (MQ=255)
                                                                            ctgctgCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAaca                                                         >  8:131182/1‑135 (MQ=255)
                                                                               ctgcAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAAcaca                                                       <  4:23729/134‑1 (MQ=255)
                                                                                  cAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCATCGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCACCACATCAc                                                   >  3:214347/1‑135 (MQ=255)
                                                                                  cAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCAc                                                   >  2:475/1‑135 (MQ=255)
                                                                                         cAGTGCCTGGGAAAGGAATGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAgccgcc                                           <  6:68854/136‑1 (MQ=255)
                                                                                            tGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGATGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGc                                        <  5:203759/136‑1 (MQ=255)
                                                                                                     aaGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAActgc                               <  8:194155/136‑1 (MQ=255)
                                                                                                     aaGGACTGACAACCCCTCCAATGCAGTCGGGATGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAActgc                               <  8:78754/136‑1 (MQ=255)
                                                                                                      aGGACTGACAACCCCTCCAATGCAGTCGGGATGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAActgct                              <  3:297632/136‑1 (MQ=255)
                                                                                                          cTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTg                          <  6:286792/136‑1 (MQ=255)
                                                                                                          cTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTg                          <  7:95798/136‑1 (MQ=255)
                                                                                                               aaCCCCTCCAATGCAGGCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTGCCAAt                     <  4:93609/136‑1 (MQ=255)
                                                                                                                aCCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTGCCAAt                     >  5:60389/1‑135 (MQ=255)
                                                                                                                      ccAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAAcc                                                                                                <  6:223471/54‑1 (MQ=255)
                                                                                                                      ccAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAAcc                                                                                                >  5:223471/1‑54 (MQ=255)
                                                                                                                                 ggggTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTGCCAATGAGGTGACCATTCCGCtt   <  5:116517/136‑1 (MQ=255)
                                                                                                                                  gggTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCCCATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTGCCAATGAGGTGACCATTCCGCTTg  >  1:124413/1‑136 (MQ=255)
                                                                                                                                  gggTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTGCCAATGAGGTGACCATTCCGCTTg  >  3:266163/1‑136 (MQ=255)
                                                                                                                                  gggTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTGCCAATGAGGTGACCATTCCGCTTg  >  5:281633/1‑136 (MQ=255)
                                                                                                                                  gggTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTGCCAATGAGGTGACCATTCCGCTTg  >  5:61891/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                                     
ACCCATGCACAGCCATCGCTCAGCCGTTGGGAAATGGAACGGGCATCAGGCCGTAGCGCGAAGCGCCAATCTTGTCCTGCTGCAGCTGGCAGTGCCTGGGAAAGGACTGACAACCCCTCCAATGCAGTCGGGGTGTACTCCGTAACTGGGAGCCCAGCCAACTCAACGAACCGATCAGCCGCCCGCTGCACATCTAGCACGACAGGCAACACATCACGAGCCGCCTGCACGAACTGCTGTTGCCAATGAGGTGACCATTCCGCTTG  >  ACB143/4198477‑4198742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑