| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 4,624,111 | C→A | 16.4% | intergenic (+117/+181) | CJ019_04367 → / ← pgsA | hypothetical protein/CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,624,111 | 0 | C | A | 16.4% | 70.3 / 2.7 | 34 | intergenic (+117/+181) | CJ019_04367/pgsA | hypothetical protein/CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (18/9); new base A (2/4); total (21/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTG > ACB143/4623977‑4624241 | aGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCTTTGTGGTGTTTGTACTTGAATTTCGTAGATTGCGGTGTATTTAGGCATAATGCATTCTAACGAAAAAAGGCCCCGAAGGGCCTTTTTTc < 3:82587/135‑1 (MQ=255)aGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTc > 1:122847/1‑135 (MQ=255) gCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCaa < 3:65132/136‑1 (MQ=255) ttCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTTTGGTGTTTGTAGTTTAATTTCGTAGAGGGCGGGGTTTTGAGGCAGAATGCATTCGAACGAAAACAGGCCCCGAAGGGCCTTTTTTCAACGACtt < 2:269890/134‑1 (MQ=255) ccAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTa > 6:280416/1‑134 (MQ=255) aGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAACGCATTCGAACGAAAAAAGGCCCCGACGGGCCTTTTTTCAACGACTTACAGa < 1:5356/136‑1 (MQ=255) tAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACtt > 3:260239/1‑136 (MQ=255) cACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACt > 1:17213/1‑136 (MQ=255) cGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAAc > 2:89814/1‑136 (MQ=255) tCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCt < 1:82475/136‑1 (MQ=255) ggCCAGGGCGCCGCGTTGTGGTGGTTGTACTTTAATTTCGTAGAGGGGGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGTAGGGCCTTTTTTGAACGACATACACACTTCGACTGACACCTg > 3:32036/1‑133 (MQ=255) cgTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTAGACTGAAACCTGTAGATCTTTATTTg > 5:175362/1‑134 (MQ=255) gTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACgaga > 5:133350/1‑136 (MQ=255) gTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCAGAAGGGCCTTTTTTCAACGACTTACAGACGTCGCCTGAAACATGTAGATCTTTATTTGGCGCTGGACACtagc > 5:188259/1‑135 (MQ=255) aTTTCGTAGAGGGCGGGGTATTCAGGCAGAATGCATTCGAACGAAAAAAGGCACCGAAGGGCCTTTTTTAAACGACTTACAGACTTCGACTGAACCCTGAAGATCTTTATTTGGAGCGGGAAACGCGACTCGAACt > 6:171564/1‑136 (MQ=255) ggggTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGATACGAGACTCGAACTCGCGACCCCGACCt > 5:234019/1‑136 (MQ=255) ggggTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCt > 4:247038/1‑136 (MQ=255) aTTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGc > 6:8392/1‑135 (MQ=255) aGGCAGAATGAATGCGAACTAAAAACGCCCCCGAAGGGCTTTTTTTAAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGt < 8:213489/136‑1 (MQ=25) gattGCATTCTAACTAAAAATGGCCCCTATGGGCTTTTTTTAAACGACTTACAGACTTCGACTGAAACCTGTATATATTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc < 5:104014/133‑1 (MQ=21) gAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc < 3:247038/136‑1 (MQ=255) tGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAg < 7:276621/85‑1 (MQ=255) tGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAg > 8:276621/1‑85 (MQ=255) tGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGTAAACGAGACTCGAACTCGCGACCCCGACATTGGCAAGGTCGTGctc > 6:112212/1‑135 (MQ=255) tGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTGctc > 1:58265/1‑135 (MQ=255) tGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGctc > 5:267043/1‑135 (MQ=255) cgaacgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGCCTCGAACTCGCGACCCCGCCCTTGGCAAGGACGTGCACTACCAAc > 4:218190/1‑136 (MQ=255) caactaaAAAAGCCCCCTAGGGGCTTTTTTTAAACGACTTACCGACTTCGACTTAAACCTGTATATCTTTATTTGGCGCGGGAACCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt < 5:1152/135‑1 (MQ=18) actaaaaaaGGCCCCTAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTTAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg < 1:208957/132‑1 (MQ=255) gaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAACCCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCACCTGAGc > 2:101286/1‑136 (MQ=255) gaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGc > 5:121431/1‑136 (MQ=255) gaaAAAAGGCCCCGAAGGGCCTATTTTAATCGACTTACAGACTTCGACTGAAACATGAAGATCTTTATATGGACCGGGAAACGAGACTCGAACTAGCGACCCCGACGTTGGCAAGGTCGTGCTCTACCAACTGAGc > 1:19822/1‑136 (MQ=21) aGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgtcgt < 7:94254/135‑1 (MQ=255) cTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATggg < 7:103222/136‑1 (MQ=255) tttttCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTg > 4:89463/1‑136 (MQ=255) tttttAAACGACTTAAATACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATggg < 7:214189/134‑1 (MQ=255) | AGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGTGATGGGTG > ACB143/4623977‑4624241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |