Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 343,673 T→C 54.1% G12G (GGT→GGC)  hisH → Imidazole glycerol phosphate synthase subunitHisH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143343,6730TC54.1% 17.2 / 83.2 61G12G (GGT→GGC) hisHImidazole glycerol phosphate synthase subunitHisH
Reads supporting (aligned to +/- strand):  ref base T (17/11);  new base C (17/16);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

ACCGTATTCAAGGCTTTCGGCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGCGTGGGCGCGATTCGCGACTGCATGGC  >  ACB143/343542‑343807
                                                                                                                                   |                                                                                                                                      
aCCGTATTCAAGGCTTTCGGCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAAcc                                                                                                                                    <  6:173817/136‑1 (MQ=255)
 ccGTATTCAAGGCTTTCGGCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCt                                                                                                                                   <  8:61849/136‑1 (MQ=255)
 ccGTATTCAAGGCTTTCGGCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCt                                                                                                                                   >  7:52607/1‑136 (MQ=255)
      ttCAAGGCTTTCGGCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCAc                                                                                                                               <  8:249048/135‑1 (MQ=255)
          aGGCTTTCGGCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGt                                                                                                                          <  4:299032/136‑1 (MQ=255)
            gCTTTCGGCCGCGCGCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTgg                                                                                                                        <  6:154466/136‑1 (MQ=255)
              tttCGGCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGc                                                                                                                       >  4:32267/1‑135 (MQ=255)
                tCGGCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGGGCCTGTAAATGCAAACGGGAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGcc                                                                                                                      <  5:10604/134‑1 (MQ=255)
                   gCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGgc                                                                                                                 >  5:289898/1‑136 (MQ=255)
                          tctGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAg                                                                                                          >  2:3947/1‑136 (MQ=255)
                              cgcATGGCCATCACCCTCGACGAGCGTATGGAGGTGCTGATGCCGTCCACCATAGGGTTACTGTAAAGGCAATGGTTAGGCTTAATCGACTCTGGCATGGGCATGCTGCTCTCTGTGGGCAAGGCGCTTGAGc                                                                                                         <  8:199965/133‑1 (MQ=255)
                                 aTGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAg                                                                                                   >  5:147482/1‑136 (MQ=255)
                                      cATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGcc                                                                                              >  8:59562/1‑136 (MQ=255)
                                       aTCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCg                                                                                             <  2:27864/136‑1 (MQ=255)
                                       aTCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCg                                                                                             <  5:203674/136‑1 (MQ=255)
                                            ccTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTaaa                                                                                        <  5:312559/136‑1 (MQ=255)
                                            ccTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTaaa                                                                                        <  8:180369/136‑1 (MQ=255)
                                            ccTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTaaa                                                                                        <  1:93188/136‑1 (MQ=255)
                                            ccTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTaaa                                                                                        <  2:51657/136‑1 (MQ=255)
                                                 acgaGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCt                                                                                   <  6:159724/136‑1 (MQ=255)
                                                 acgaGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCt                                                                                   >  8:18166/1‑136 (MQ=255)
                                                 acgaGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCt                                                                                   >  2:16554/1‑136 (MQ=255)
                                                  cgaGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTg                                                                                  >  3:40236/1‑136 (MQ=255)
                                                  cgaGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTg                                                                                  >  7:197596/1‑136 (MQ=255)
                                                    aGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGt                                                                                <  6:286793/136‑1 (MQ=255)
                                                         aTGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTAc                                                                             >  5:96593/1‑134 (MQ=255)
                                                          tGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAg                                                                          <  2:36304/136‑1 (MQ=255)
                                                            gCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGc                                                                         >  8:206817/1‑135 (MQ=255)
                                                                   aGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCt                                                                  >  4:74278/1‑135 (MQ=255)
                                                                   aGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCt                                                                  >  6:43700/1‑135 (MQ=255)
                                                                         gttCCACCAACGGGTGCCTGTAAATGCAAACGGTCGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGAt                                                           <  2:320198/135‑1 (MQ=255)
                                                                          ttCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATc                                                          >  8:31572/1‑136 (MQ=255)
                                                                             caccaAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCcgc                                                       >  2:198671/1‑136 (MQ=255)
                                                                             caccaAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCcgc                                                       >  7:190258/1‑136 (MQ=255)
                                                                              accaAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCcgc                                                       >  1:220590/1‑135 (MQ=255)
                                                                               ccaAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGa                                                     <  4:247222/136‑1 (MQ=255)
                                                                               ccaAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGa                                                     <  5:78107/136‑1 (MQ=255)
                                                                                  aatggTGCCTGTCACTGCAAACGGTCGCCGTAATCGACTATGCCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGAACGTAGGTGCCGGTAAAGTGCTGGGTACCAGCGATGCCTCAGTGATCCGCGAGGc                                                  <  7:234247/133‑1 (MQ=255)
                                                                                  aaGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGc                                                  <  2:46482/136‑1 (MQ=255)
                                                                                  aaGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGc                                                  >  3:150378/1‑136 (MQ=255)
                                                                                  aaGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGc                                                  >  4:166970/1‑136 (MQ=255)
                                                                                      gTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGAc                                              >  3:99597/1‑136 (MQ=255)
                                                                                         ccTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCgt                                           <  7:36281/136‑1 (MQ=255)
                                                                                           tGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCgtgt                                         >  4:82058/1‑136 (MQ=255)
                                                                                            gTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGtgtg                                        >  5:7605/1‑136 (MQ=255)
                                                                                            gTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGtgtg                                        >  5:214765/1‑136 (MQ=255)
                                                                                            gTAAATGCAAACAGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGtgtg                                        >  3:182023/1‑136 (MQ=255)
                                                                                                  gCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCAGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTc                                  >  7:211631/1‑136 (MQ=255)
                                                                                                    aaaCGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTccc                                >  7:156329/1‑136 (MQ=255)
                                                                                                    aaaCGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTccc                                >  6:157196/1‑136 (MQ=255)
                                                                                                      aCGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCgg                              <  6:7605/136‑1 (MQ=255)
                                                                                                        ggTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGc                            >  8:104475/1‑136 (MQ=255)
                                                                                                        ggTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGc                            >  5:63720/1‑136 (MQ=255)
                                                                                                        ggTAGCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGc                            >  4:40189/1‑136 (MQ=255)
                                                                                                            gCCGTAATCGACTATGGCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGCgggg                        <  6:41474/136‑1 (MQ=255)
                                                                                                                       cTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGCGTGGGCGCGATTcg              <  4:6066/135‑1 (MQ=255)
                                                                                                                           ggCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGACGACCGTGTGGTGTTACCGGTCGTGGGCGCGATTCGCGACt         >  6:96649/1‑136 (MQ=255)
                                                                                                                            gCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGCGTGGGCGCGATTCGCGACt         <  2:206499/135‑1 (MQ=255)
                                                                                                                            gCATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGAGCCGCGAGGCCGACCGTGTGGTGTTCCCGGGCGTGGGCGCGATTCGCGACt         <  1:131368/135‑1 (MQ=255)
                                                                                                                             cATGGGCAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGCGTGGGCGCGATTCGCGACTg        >  4:288147/1‑135 (MQ=255)
                                                                                                                                  gTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGCGTGGGCGCGATTCGCGACTGCATGGc  >  5:292256/1‑136 (MQ=255)
                                                                                                                                   |                                                                                                                                      
ACCGTATTCAAGGCTTTCGGCCGCGCTCTGCGCATGGCCATCACCCTCGACGAGCGTATGGCCGGGCAGATGCCTTCCACCAAAGGGTGCCTGTAAATGCAAACGGTAGCCGTAATCGACTATGGCATGGGTAACCTGCACTCGGTGGCCAAGGCGCTTGAGCACGTAGGTGCCGGTAAAGTGCTGGTTACCAGCGATGCCTCAGTGATCCGCGAGGCCGACCGTGTGGTGTTCCCGGGCGTGGGCGCGATTCGCGACTGCATGGC  >  ACB143/343542‑343807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑