Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	4,759,606	G→A	100%	A28T (GCC→ACC)	ligM_KToptimized →	sequence generated by OPTIMIZER for KT2440

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,759,606	0	G	A	99.1%	275.9 / ‑5.9	84	A28T (GCC→ACC)	ligM_KToptimized	sequence generated by OPTIMIZER for KT2440
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (60/23);  total (61/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTAA  >  ACB143/4759480‑4759730                                                                                                                               |                                                                                                                             gCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTc                                                                                                                      >  6:3671/1‑135 (MQ=255) gCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTc                                                                                                                      >  7:123040/1‑135 (MQ=255)           aTTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGgtcgt                                                                                                           >  7:188129/1‑136 (MQ=255)           aTTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGgtcgt                                                                                                           >  7:272028/1‑136 (MQ=255)                  acCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCgcgc                                                                                                      >  2:280927/1‑134 (MQ=255)                        acaTAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTtc                                                                                              <  4:149078/136‑1 (MQ=255)                        acaTAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTtc                                                                                              <  3:189705/136‑1 (MQ=255)                         caTAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTtct                                                                                             >  6:254820/1‑136 (MQ=255)                          aTAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGAAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGACTtct                                                                                             >  6:199487/1‑135 (MQ=255)                             gtgTTTAGGAGGTATACATGAGCGCCCCGACTACCCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa                                                                                         <  5:180000/134‑1 (MQ=255)                              ggTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGCTTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa                                                                                         <  6:297423/135‑1 (MQ=255)                              ggTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa                                                                                         <  1:280927/135‑1 (MQ=255)                               gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTTGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACt                                                                                       >  6:211120/1‑136 (MQ=255)                               gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACt                                                                                       >  4:109699/1‑136 (MQ=255)                                 ttAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTgg                                                                                     >  6:234331/1‑136 (MQ=255)                                   aggaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGc                                                                                    <  2:160348/135‑1 (MQ=255)                                   aggaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCg                                                                                   >  8:109689/1‑136 (MQ=255)                                    ggaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGt                                                                                  >  7:145750/1‑136 (MQ=255)                                    ggaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCg                                                                                   >  6:258239/1‑135 (MQ=255)                                    ggaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGt                                                                                  <  7:266356/136‑1 (MQ=255)                                     gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa                                                                                 >  3:114465/1‑136 (MQ=255)                                     gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa                                                                                 >  3:214996/1‑136 (MQ=255)                                     gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa                                                                                 >  3:282563/1‑136 (MQ=255)                                     gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa                                                                                 >  8:214918/1‑136 (MQ=255)                                     gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa                                                                                 >  7:117868/1‑136 (MQ=255)                                     gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa                                                                                 >  6:124046/1‑136 (MQ=255)                                     gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa                                                                                 >  8:29411/1‑136 (MQ=255)                                           taCATGAGCGCCCCGACTACCCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAAc                                                                           <  1:167917/136‑1 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCATGTGCTGGCCGCCGGCGGAAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                            >  6:288546/1‑135 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                            >  1:183401/1‑135 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                            >  6:229233/1‑135 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                            >  1:200241/1‑135 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                            >  8:111481/1‑135 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                            >  1:297749/1‑135 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                            >  5:219287/1‑135 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                            >  5:215014/1‑135 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                            >  1:304533/1‑135 (MQ=255)                                           taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTAAGTCTACCCGGTCGTAGCGACACAGTTCTCTAACTGGAGTACCGacc                                                                           >  2:250743/1‑134 (MQ=255)                                             cATGCGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAAc                                                                           <  3:112582/134‑1 (MQ=255)                                             cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAg                                                                         >  1:192746/1‑136 (MQ=255)                                             cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAg                                                                         >  3:165066/1‑136 (MQ=255)                                             cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAg                                                                         >  2:68383/1‑136 (MQ=255)                                             cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAg                                                                         >  6:167854/1‑136 (MQ=255)                                             cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTACCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGCACAg                                                                         >  8:69141/1‑136 (MQ=255)                                                     ccccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTg                                                                 >  7:36455/1‑136 (MQ=255)                                                     ccccGACCAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTg                                                                 >  1:2540/1‑136 (MQ=255)                                                      cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg                                                                >  1:173460/1‑136 (MQ=255)                                                      cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg                                                                >  5:22927/1‑136 (MQ=255)                                                      cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg                                                                >  2:291418/1‑136 (MQ=255)                                                      cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg                                                                >  7:26269/1‑136 (MQ=255)                                                      cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg                                                                >  1:172240/1‑136 (MQ=255)                                                      cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg                                                                >  3:213686/1‑136 (MQ=255)                                                      cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg                                                                >  3:131293/1‑136 (MQ=255)                                                      cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACAGGCGTACCGCACAGTGGGCTTgg                                                                >  2:109085/1‑136 (MQ=255)                                                       ccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgc                                                               >  5:88569/1‑136 (MQ=255)                                                        cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGCCCAGTGGGCTTGgcg                                                              >  8:217553/1‑136 (MQ=255)                                                        cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                              >  6:293895/1‑136 (MQ=255)                                                        cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                              >  3:30434/1‑136 (MQ=255)                                                        cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCAAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                              >  2:86160/1‑136 (MQ=255)                                                          aCTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCa                                                            <  6:292417/136‑1 (MQ=255)                                                             aaCCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACt                                                         <  6:233782/136‑1 (MQ=255)                                                                           gCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTc                                           >  7:186975/1‑136 (MQ=255)                                                                            cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTc                                           >  6:65037/1‑135 (MQ=255)                                                                            cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCg                                          <  2:144051/136‑1 (MQ=255)                                                                            cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCg                                          >  1:110478/1‑136 (MQ=255)                                                                            cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTAACGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTc                                           >  8:280147/1‑135 (MQ=255)                                                                               gccgccGGGGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGAc                                        <  8:50039/135‑1 (MQ=255)                                                                                 ctccgGCGGCAACACAGTTGAAATTTTTCGTAACTCCCAGATTGGCAGCTACGTCTTCCCTGTAGTCGAGGCAGTGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTTCTGTTCGACCaa                                     <  3:10536/134‑1 (MQ=255)                                                                                 cgccgGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                              >  2:56726/1‑111 (MQ=255)                                                                                 cgccgGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                              <  1:56726/111‑1 (MQ=255)                                                                                      gcggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACcca                                >  2:29524/1‑136 (MQ=255)                                                                                      gcggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACcca                                <  3:34720/136‑1 (MQ=255)                                                                                        ggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACccacc                              <  2:192746/136‑1 (MQ=255)                                                                                            acacCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACAt                          <  7:111481/136‑1 (MQ=255)                                                                                              acCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACAtgg                        <  8:186975/136‑1 (MQ=255)                                                                                                cGTTGAAATGTTGCGTAACTCCCAGTTTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACAtggtg                      <  6:182375/136‑1 (MQ=255)                                                                                                    gAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCTTGCTGTTCGACCAAACCCCCCACATGGTGGAcc                  >  3:208836/1‑136 (MQ=255)                                                                                                    gAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGAcc                  >  7:198139/1‑136 (MQ=255)                                                                                                     aaaTGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCt                 >  4:123408/1‑136 (MQ=255)                                                                                                          ttGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTAc             <  3:144591/135‑1 (MQ=255)                                                                                                             cGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATcc         >  2:252470/1‑136 (MQ=255)                                                                                                             cGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATcc         >  4:233213/1‑136 (MQ=255)                                                                                                                     ccAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTaa  <  1:291418/135‑1 (MQ=255)                                                                                                                     ccAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTaa  <  4:64353/135‑1 (MQ=255)                                                                                                                               |                                                                                                                             GCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTAA  >  ACB143/4759480‑4759730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑