Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,327,077 T→C 25.1% L63P (CTG→CCG)  sasA_18 → Adaptive‑response sensory‑kinase SasA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,327,0770TC25.1% 74.4 / 27.8 44L63P (CTG→CCG) sasA_18Adaptive‑response sensory‑kinase SasA
Reads supporting (aligned to +/- strand):  ref base T (21/11);  new base C (6/5);  total (27/17)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01

ATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCACCCGCACCTATCAGCTGTCATCGCTGGCCGACCT  >  ACB143/5326944‑5327209
                                                                                                                                     |                                                                                                                                    
aTGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCGTCTATGGCTCGGTtggcctgg                                                                                                                                    >  4:190102/1‑136 (MQ=255)
  ggTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggcctggcc                                                                                                                                  >  7:144517/1‑136 (MQ=255)
   gTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggcctggcc                                                                                                                                  <  7:286804/135‑1 (MQ=255)
   gTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggcctggcc                                                                                                                                  >  5:10201/1‑135 (MQ=255)
   gTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCAGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGACTGGGCCTTCAATGGCGCCGTtgggcaggcc                                                                                                                                  >  5:10204/1‑135 (MQ=255)
      cTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTAcc                                                                                                                              <  1:119812/136‑1 (MQ=255)
       tGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggcccggcc                                                                                                                                  <  7:107457/131‑1 (MQ=255)
       tGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTtggcccggcc                                                                                                                                  >  8:107457/1‑131 (MQ=255)
       tGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTAc                                                                                                                               >  1:101726/1‑134 (MQ=255)
         ttCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTACCAg                                                                                                                            >  4:26338/1‑135 (MQ=255)
            ggCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacg                                                                                                                        >  4:115474/1‑136 (MQ=255)
            ggCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGtacg                                                                                                                        >  4:67436/1‑136 (MQ=255)
                      ggATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCtt                                                                                                              <  5:313224/136‑1 (MQ=255)
                         tCAGCGATCGCGGGCGGATCCCGCGTTCGATCATTCGCGACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGTGCATTCTATGGCTCGGTTGGACTTGCCTACCAGTACGGCTACTGCTTCCt                                                                                                           <  3:218632/136‑1 (MQ=255)
                         tCAGCGAGCGCGGGCTGATCCCGCGTTCGATCTTTCGCGACCCACTTACCTACACGCTGTCGCTGGGCGTCTACGGCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCt                                                                                                           <  3:222950/136‑1 (MQ=255)
                            gcgagcgCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGc                                                                                                        <  2:268250/136‑1 (MQ=255)
                            gcgagcgCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTACCAGTACGGCTACGGCTTCCTTGc                                                                                                        <  5:85281/136‑1 (MQ=255)
                            gcgagcgCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTACCAGTACGGCTACGGCTTCCTTGc                                                                                                        <  1:248697/136‑1 (MQ=255)
                               agcgCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTg                                                                                                     >  2:61537/1‑136 (MQ=255)
                               agcgCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTg                                                                                                     >  7:17757/1‑136 (MQ=255)
                                             ccGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGgtgt                                                                                       >  5:222864/1‑136 (MQ=255)
                                                       tCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCAtt                                                                             >  4:315660/1‑136 (MQ=255)
                                                          ttCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCct                                                                          <  4:150240/136‑1 (MQ=255)
                                                                    acTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCg                                                                <  6:153641/136‑1 (MQ=255)
                                                                    acTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCg                                                                <  5:191451/136‑1 (MQ=255)
                                                                         ccTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGtgct                                                           <  5:221639/136‑1 (MQ=255)
                                                                            acacCCTGTCGCTGGGCGTCTAAGCCACTGCCTGGGCCTTCTATTGCTCGGTTTGCCTGGCCTACCAGTACGGCTACCGCTTCCTTGCCTGTTACCAGGGGGTGTATGGCGCATTACTGCTGGCGCCCGtgctgcg                                                        >  6:39644/1‑135 (MQ=255)
                                                                                ccTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTAc                                                    <  1:130763/136‑1 (MQ=255)
                                                                                     cGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCg                                                                >  5:168530/1‑119 (MQ=255)
                                                                                     cGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCg                                                                <  6:168530/119‑1 (MQ=255)
                                                                                     cGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCgct                                               >  7:77104/1‑134 (MQ=255)
                                                                                     cGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGAt                                               >  2:4818/1‑136 (MQ=255)
                                                                                     cGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGAt                                               >  8:103000/1‑136 (MQ=255)
                                                                                     cGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGAt                                               >  5:256866/1‑136 (MQ=255)
                                                                                     cGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGAt                                               >  3:257845/1‑136 (MQ=255)
                                                                                     cGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGAt                                               >  4:314897/1‑136 (MQ=255)
                                                                                     cGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGAt                                               >  8:188056/1‑136 (MQ=255)
                                                                                       cTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACGTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGGTGGTCGTCCGGATcc                                             >  2:107960/1‑136 (MQ=255)
                                                                                       cTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATc                                              >  4:235646/1‑135 (MQ=255)
                                                                                       cTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCGGGGGGTGTCTGGCGCATTACTGCTGGCGCCGGTGCTGCTCTACCCGCTc                                              >  5:189729/1‑135 (MQ=255)
                                                                                       cTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCCGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATcc                                             >  3:300550/1‑136 (MQ=255)
                                                                                       cTGGGCGTCTACGCCAGTGCCTGGGCCATCTATGGCTCCGTTGGCCCGGCCTACCAGTACCGCTACCGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTACTGCTGGCGCCGGTGCTGCTCTACCCGATcc                                             >  4:312778/1‑136 (MQ=255)
                                                                                             gTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTACTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCCCCGGTGCTGGGCTGCCCGATCCTc                                           >  2:220991/1‑132 (MQ=255)
                                                                                                  cGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCAc                                   <  7:43517/135‑1 (MQ=255)
                                                                                                  cGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCAc                                   <  5:249575/135‑1 (MQ=255)
                                                                                                           cTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTAACCTGGGCGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCACCCGCACCTa                          >  1:108335/1‑135 (MQ=255)
                                                                                                                     tATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGGGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCACCCGCACCTATCAGCTGTCAt               >  3:266878/1‑136 (MQ=255)
                                                                                                                          cTCGGTTGGCCGGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCACCCGCACCTATCAGCTGTCATCGCTg          <  5:305299/136‑1 (MQ=255)
                                                                                                                            cGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTAACCGATCCTCAAGATCACCCGCACCTATCAGCTGTCATAGCTGGc        >  4:314013/1‑136 (MQ=255)
                                                                                                                             ggTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCACCCGCACCTATCAGCTGTCATCGCTgg         <  2:259516/134‑1 (MQ=255)
                                                                                                                               ttGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCACCCGCACCTATCAGCTGTCATCGCTGGCCGa     >  7:5811/1‑136 (MQ=255)
                                                                                                                                tggcctggccTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCACCCGCACCTATCAGCTGTCATCGCTGGCCGAc    >  4:179537/1‑136 (MQ=255)
                                                                                                                                 ggcctggcctACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAACATCACCCGCACCTATCAGCTGTCATCTCTGGCCGAcc   >  4:83985/1‑136 (MQ=255)
                                                                                                                                  gcctggcctACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCACCCGCACCTATCAGCTGTCATCGCTGGCCGACct  >  6:220315/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                    
ATGGTGCTGTTCGGCGTGGCCTGGATCAGCGAGCGCGGGCTGATCCCGCGTTCGATCATTCGCCACCCACTTACCTACACCCTGTCGCTGGGCGTCTACGCCAGTGCCTGGGCCTTCTATGGCTCGGTTGGCCTGGCCTACCAGTACGGCTACGGCTTCCTTGCCTGTTACCTGGGGGTGTCTGGCGCATTCCTGCTGGCGCCGGTGCTGCTCTACCCGATCCTCAAGATCACCCGCACCTATCAGCTGTCATCGCTGGCCGACCT  >  ACB143/5326944‑5327209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑