| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,334,186 | A→T | 14.6% | intergenic (+236/‑27) | folK → / → panB | 2‑amino‑4‑hydroxy‑6‑hydroxymethyldihydropteridin e pyrophosphokinase/3‑methyl‑2‑oxobutanoatehydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,334,186 | 0 | A | T | 14.6% | 158.0 / 4.5 | 78 | intergenic (+236/‑27) | folK/panB | 2‑amino‑4‑hydroxy‑6‑hydroxymethyldihydropteridin e pyrophosphokinase/3‑methyl‑2‑oxobutanoatehydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (38/28); new base T (10/2); total (48/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGGCGTCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCTGACCTGCTACGACGCGACCTTCGCCAAGGCCGCCAGCCA > ACB143/5334056‑5334316 | taGGCGTCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCTCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAg < 4:305915/134‑1 (MQ=255) gCGTCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCATTCGTTTTTTTCACAGCCt > 5:143190/1‑136 (MQ=255) gCGTCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTAAAGCGCCt > 4:132015/1‑136 (MQ=255) ccGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTTCAGCGCCGGAAc > 7:273724/1‑135 (MQ=255) ccGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAAc > 7:37778/1‑135 (MQ=255) ccGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAAc < 5:88333/135‑1 (MQ=255) ccGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAAc > 4:86777/1‑135 (MQ=255) ccGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCGGAACg > 7:26000/1‑136 (MQ=255) cGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAAc > 1:55984/1‑134 (MQ=255) cGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTACAACGCCTGAAc > 5:279220/1‑134 (MQ=255) gtgtGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTTCAGCGCCTGAACGAg > 1:251952/1‑136 (MQ=255) gtgtGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAg > 6:211764/1‑136 (MQ=255) gtgtGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAg > 7:228679/1‑136 (MQ=255) gtgtGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCGGAACGAg > 3:144663/1‑136 (MQ=255) tgtgGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAg > 4:301244/1‑135 (MQ=255) tgtgGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTACAGCGCCTGAACGAg > 2:192371/1‑135 (MQ=255) tgtgGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATAACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTACAGCGGCTGGACGCgg > 6:29343/1‑136 (MQ=255) ggCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGACGAAt > 5:314336/1‑136 (MQ=255) aCCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTc < 6:304568/136‑1 (MQ=255) aCCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTc < 1:265876/136‑1 (MQ=255) ccAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTAAAGCGGCTGAACGAGGAAattag > 7:121034/1‑134 (MQ=255) aaaGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTTAAGCGCCTGAACGAGGAATTTCCt > 6:279295/1‑135 (MQ=255) gttccGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACAt < 6:301242/133‑1 (MQ=255) gTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACAt < 5:51407/136‑1 (MQ=255) gTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACAt < 8:114822/136‑1 (MQ=255) gTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACAt < 3:301244/136‑1 (MQ=255) gTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACAt < 8:31796/136‑1 (MQ=255) gTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACAt < 1:306822/136‑1 (MQ=255) cATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTTAAGCGCCTTAACGATGAATTTCATACATGCCTg > 3:17065/1‑135 (MQ=255) cATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACATCGCCGGAAAGAGGGATTTACTAAATTccag > 2:277046/1‑133 (MQ=255) cATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTACAGCGCCGGAACCAGGTATTTCCTAAAAGCCTg > 1:92495/1‑135 (MQ=255) cATACCCGTATTTAGGCCCGGAAGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTGTTTTCCGCGCCTGACCGAGGACTTTCCGAAATGCCTg > 4:258796/1‑135 (MQ=255) aTACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTTCTGATTCTTAACTAGTAATTTCCTACATGCCTgta < 4:297322/136‑3 (MQ=255) aTACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGaa < 2:148595/136‑1 (MQ=255) tATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAAccc < 8:282633/136‑1 (MQ=255) tATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTACAGCGCCTGCACGCGGGATTTCCTACATGCCTGCAGTAAccc > 1:266013/1‑136 (MQ=255) tttAGGCCCGGACGGACCTGTGGCCGAACATCACGCGCGATGATGTGCCGCTCCGGATGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCtt < 6:133971/136‑1 (MQ=255) tttAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCt < 6:262442/135‑1 (MQ=255) tttAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCt < 2:265742/135‑1 (MQ=255) aGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCt > 2:203066/1‑132 (MQ=255) aGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCt < 1:203066/132‑1 (MQ=255) cggacggaCCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGCACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCt > 8:33247/1‑136 (MQ=255) cggacggaCCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCt > 8:18364/1‑136 (MQ=255) cggacggaCCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCAGAACGAGGAATTTCCTACATTCCTGAAGTAACCAATACCAccca > 8:112572/1‑135 (MQ=255) cggacggaCCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTACAGCGGCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCt > 8:189673/1‑136 (MQ=255) cggacggaCCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTAAATCGCCAGTAAGAGGAATTTGCTACATGGCTGAAGTAAACCTTACCAccca > 1:145683/1‑135 (MQ=255) cggacggaCCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTAAAGCGCCTGAACGAGGAATTTACTACATTCCTGAAGTAACCCATACCACCCt > 7:187242/1‑136 (MQ=255) acggacCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGATTTTCCTACATGCCTGAAGTAACCCTTACCACCCttt > 5:117849/1‑134 (MQ=255) acggacCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTTACCCTTACCACCCTTa > 5:105112/1‑135 (MQ=255) acCTGTGCGCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTTCAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGc < 7:195150/136‑1 (MQ=255) acCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGc < 8:83710/136‑1 (MQ=255) tgtgCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCt > 1:86172/1‑135 (MQ=255) cATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTTCAGCGGCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAgg > 7:281932/1‑136 (MQ=255) cATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTTCAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAAAGGCCTCAAGGCCAAgg > 3:291582/1‑136 (MQ=255) cATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAgg > 6:47061/1‑136 (MQ=255) aTCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTTAAGCGCCTGAACGAGGAATTTACTACATGCCTGAAGTAAACCTTACCCCCCTTAACCGCCTCAAGGCCAAggg > 1:272120/1‑136 (MQ=255) tCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACCAGGAATTTCCTACATGCCAGAAGTAAACCTTAACACCCTTAACGGCCGCAAGGCCAACGGt > 5:19503/1‑136 (MQ=255) aCGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAAAGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGa < 4:319426/136‑1 (MQ=255) cgcgcgATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGa > 5:52327/1‑135 (MQ=255) cgcgcgATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTTAAGCGCCTGAACGAGGAATTTACTAACTGCCTGAAGTAACCCTTACCACCCATAACCGCCGCAAGGCCAACGGTGa > 6:198230/1‑135 (MQ=255) cgcgATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGaaaa < 4:214260/136‑1 (MQ=255) cgATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGaaaaaa < 3:135732/136‑1 (MQ=255) atgatgTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAAt > 6:136469/1‑135 (MQ=255) tgatgTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTGTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAATCa > 2:191790/1‑136 (MQ=255) ccGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCt < 3:41023/136‑1 (MQ=255) ccGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCt < 3:146331/136‑1 (MQ=255) gATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCTGACCTGCTacg < 4:223496/136‑1 (MQ=255) gATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCTGACCTGCTacg < 8:228679/136‑1 (MQ=255) tACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCATAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCTGACCTGCTACGACGCGa > 3:176417/1‑136 (MQ=255) cacGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAAccc < 4:151901/68‑1 (MQ=255) cacGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAAccc > 3:151901/1‑68 (MQ=255) cacGCGTTGAATGCAGTCGTTTTTTACAGCGCCGGAACGAGGAATTTCCTACATGCCTGAAGTAAACCGTACCACCCTTAACGGCCGCAACGCCAAGGGTGAAAAAATAACCATGCGGAACTGCTACGACGCGAc > 2:127832/1‑135 (MQ=255) gcgTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCt > 4:189528/1‑66 (MQ=255) gcgTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCt < 3:189528/66‑1 (MQ=255) aaTGCAGTCGTTTTTTACAGCGCCAGAACGAGGAATTTCCTACATGCCGGTAGTAACCCTTAACACCCTTAACGGCCTCAACGCCAAGGGGGAAAAAATCACCATGCTGACCTGCTACGACGCGCCCTTCGCCaac > 3:293107/1‑135 (MQ=255) gTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCTGACCTGCTACGACGCGACCTTCGCCAAGgccgcc < 3:40195/136‑1 (MQ=255) tCGTTTTTTACAGCGCCTGAAAGAGGAATTTCCTACATTCCTGAAGTAACCCATACCACCCTTAACGGCCTCAAGGCCAACGGTGAAAAAATCACCATGCTGACCTGCTACGACGCGACCTTCGCCAAGGCCgcca > 4:62731/1‑136 (MQ=255) ttttACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCTGACCTGCTACGACGCGACCTTCGCCAAGGCCgccagcca < 1:191790/135‑1 (MQ=255) | TAGGCGTCCCGTGTGGCACCAAAGTGCCGCATACCCGTATTTAGGCCCGGACGGACCTGTGCCCGAACATCACGCGCGATGATGTGCCGCTCCGGAAGATGACTACACGCGTTGAATGCAGTCGTTTTTTACAGCGCCTGAACGAGGAATTTCCTACATGCCTGAAGTAACCCTTACCACCCTTAACGGCCTCAAGGCCAAGGGTGAAAAAATCACCATGCTGACCTGCTACGACGCGACCTTCGCCAAGGCCGCCAGCCA > ACB143/5334056‑5334316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |