Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 358,766 C→T 26.5% intergenic (‑115/‑30) CJ019_00331 ← / → cdhR_2 hypothetical protein/HTH‑type transcriptional regulator CdhR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143358,7660CT26.5% 127.9 / 49.8 72intergenic (‑115/‑30)CJ019_00331/cdhR_2hypothetical protein/HTH‑type transcriptional regulator CdhR
Reads supporting (aligned to +/- strand):  ref base C (30/22);  new base T (11/8);  total (41/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GGCGGATCAAGCTGTGCATGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGT  >  ACB143/358633‑358898
                                                                                                                                     |                                                                                                                                    
ggcggATCAAGCTGTGCATGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCg                                                                                                                                    <  7:163880/136‑1 (MQ=255)
ggcggATCAAGCTGTGCATGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCg                                                                                                                                    >  1:53272/1‑136 (MQ=255)
        aaGCTGTGCATGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGAcc                                                                                                                            <  3:190183/136‑1 (MQ=255)
        aaGCTGTGCATGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCAAGCGACAGGAATTAGCACCGGGGCGAc                                                                                                                             >  5:164351/1‑135 (MQ=255)
                 aTGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCg                                                                                                                                    >  5:139016/1‑119 (MQ=255)
                 aTGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCg                                                                                                                                    <  6:139016/119‑1 (MQ=255)
                 aTGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCtgt                                                                                                                   >  1:201477/1‑136 (MQ=255)
                 aTGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCtgt                                                                                                                   <  3:290516/136‑1 (MQ=255)
                 aTGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCtgt                                                                                                                   >  1:88741/1‑136 (MQ=255)
                        ctctCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGgaggag                                                                                                            >  8:69113/1‑136 (MQ=255)
                           tcaaTCGTCAGTTCATATTGTTTTTGAGGGCTGCCTCTGCTGACCGTGTGAATACGCTACGCTTGCGTTACCTTTCCCAATCGCACGCTAGCGACAGTAATTAGAATCTGGGCGAACAGTTTCTGTGGaggagg                                                                                                           <  4:55493/131‑1 (MQ=255)
                           tcCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAggg                                                                                                          >  7:83456/1‑135 (MQ=255)
                           tcCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAggg                                                                                                          >  3:190509/1‑135 (MQ=255)
                           tcCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAggg                                                                                                          >  6:253134/1‑135 (MQ=255)
                           tcCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGaggagg                                                                                                           <  4:317133/134‑1 (MQ=255)
                           tcCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAggg                                                                                                          >  2:170350/1‑135 (MQ=255)
                                 ggCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGgatgat                                                                                                    <  2:191396/135‑1 (MQ=255)
                                 ggCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGgatgat                                                                                                    <  2:263491/135‑1 (MQ=255)
                                 ggCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGgatgat                                                                                                    <  2:149899/135‑1 (MQ=255)
                                      tGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGAc                                                                                              <  1:170350/136‑1 (MQ=255)
                                        cATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGa                                                                                            >  1:227596/1‑136 (MQ=255)
                                           atTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTg                                                                                         >  8:276710/1‑136 (MQ=255)
                                            ttGTTATTGAGGGCGGCGTCTGCGGACCGGGTGAATACGGTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTg                                                                                         <  2:83798/135‑1 (MQ=255)
                                                   tGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTg                                                                                 >  8:27413/1‑136 (MQ=255)
                                                   tGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTg                                                                                 >  3:75357/1‑136 (MQ=255)
                                                   tGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGGTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTg                                                                                 >  4:278983/1‑136 (MQ=255)
                                                        gcggcCTCTTCGGAACGTGTGAATACTCTACGCTTGGGTTTCGTTGTCCAAGCGCAGGCTAGCGACAGGTATTAGCAAGTGGGCGACAAGTTTCTGTGGAGGAGGGGATGATGGAGACTACTGACTTTCTGTTGGt                                                                            <  1:60294/136‑1 (MQ=255)
                                                          ggcCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGGCAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGc                                                                          >  4:157463/1‑136 (MQ=255)
                                                          ggcCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTgga                                                                                                                >  8:57608/1‑98 (MQ=255)
                                                          ggcCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTgga                                                                                                                <  7:57608/98‑1 (MQ=255)
                                                          ggcCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGc                                                                          >  7:71256/1‑136 (MQ=255)
                                                          ggcCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGc                                                                          >  7:58579/1‑136 (MQ=255)
                                                          ggcCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGc                                                                          >  2:200637/1‑136 (MQ=255)
                                                          ggcCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGc                                                                          >  5:147771/1‑136 (MQ=255)
                                                           gcCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGcc                                                                         <  3:180694/136‑1 (MQ=255)
                                                                tGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCgg                                                                      <  7:43160/134‑1 (MQ=255)
                                                                tGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCgg                                                                      <  6:185916/134‑1 (MQ=255)
                                                                  cGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCCCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCttt                                                                  >  5:59425/1‑136 (MQ=255)
                                                                       cGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGc                                                             <  4:13661/136‑1 (MQ=255)
                                                                          gtgAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCAt                                                          >  4:95157/1‑136 (MQ=255)
                                                                          gtgAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCAt                                                          >  5:213256/1‑136 (MQ=255)
                                                                           tgAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATg                                                         <  5:12354/136‑1 (MQ=255)
                                                                           tgAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATg                                                         <  3:251051/136‑1 (MQ=255)
                                                                            gAATACGCTACGCTGGCGTTACCTTTCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCGTTCTGTTGGTGCCCGGCTTTTCTGCCATgg                                                        >  6:245498/1‑136 (MQ=255)
                                                                            gAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACTCTAGCGACAGGAATTAGCACCGGGGCGACCAGGTTCTGTGGAGGAGGGGATGATGGAGACGATAGCCTTTCTGTTGGTGCCCGGCTTTTCTGCGATgg                                                        >  2:288386/1‑136 (MQ=255)
                                                                             aaTACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATggg                                                       <  1:127844/136‑1 (MQ=255)
                                                                                  gctacgctGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCa                                                  >  8:270221/1‑136 (MQ=255)
                                                                                    tacgctGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTTGTGCCCGGCTTTTCTGCCATGGGGTTCAtt                                                >  2:23705/1‑136 (MQ=255)
                                                                                        ctGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCgg                                            >  8:227239/1‑136 (MQ=255)
                                                                                        ctGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCgg                                            >  7:138309/1‑136 (MQ=255)
                                                                                          ggCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCgg                                            >  4:107473/1‑134 (MQ=255)
                                                                                                   ttGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCa                                 >  6:31813/1‑136 (MQ=255)
                                                                                                   ttGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGGTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTGTCTGCCATGGGGGTGATTTCTGCAGTGGAGcaa                                 >  7:109269/1‑134 (MQ=255)
                                                                                                        cAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTg                                                              <  4:222815/102‑1 (MQ=255)
                                                                                                        cAAGCGCACGCTAGCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTg                                                              >  3:222815/1‑102 (MQ=255)
                                                                                                        cAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTGAGCCATGGGGTTCATTTCGGCAGTGGAGCCACTTCg                            >  8:265839/1‑136 (MQ=255)
                                                                                                        cAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCACGGAATTTCAGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCg                            >  6:135648/1‑136 (MQ=255)
                                                                                                              cACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGc                      >  5:12260/1‑136 (MQ=255)
                                                                                                              cACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGc                      <  1:200637/136‑1 (MQ=255)
                                                                                                                   tAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTg                                                                                 >  7:175778/1‑72 (MQ=255)
                                                                                                                   tAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTg                                                                                 <  8:175778/72‑1 (MQ=255)
                                                                                                                     gCGACAGGAATTAGCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATc                 <  3:107473/134‑1 (MQ=255)
                                                                                                                     gCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCg                <  6:213256/135‑1 (MQ=255)
                                                                                                                      cGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGtt              <  8:24316/136‑1 (MQ=255)
                                                                                                                           ggAATTAGCTCGGGGGCGACCTGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTtggt         <  7:265839/136‑1 (MQ=255)
                                                                                                                           ggAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTtggt         <  1:148844/136‑1 (MQ=255)
                                                                                                                              aTTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTc                                                                                   >  4:252/1‑59 (MQ=255)
                                                                                                                              aTTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTc                                                                                   <  3:252/59‑1 (MQ=255)
                                                                                                                              aTTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTggg      >  5:244047/1‑136 (MQ=255)
                                                                                                                                 aGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCTGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTTGGCa    <  4:75357/135‑1 (MQ=255)
                                                                                                                                 aGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCa    <  5:189951/135‑1 (MQ=255)
                                                                                                                                  gCATCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGCTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGt  >  8:41197/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GGCGGATCAAGCTGTGCATGGTGGCTCTCCATCGGCAGTGCATATTGTTATTGAGGGCGGCCTCTGCGGACCGTGTGAATACGCTACGCTGGCGTTACCTTGCCCAAGCGCACGCTAGCGACAGGAATTAGCACCGGGGCGACCAGTTTCTGTGGAGGAGGGGATGATGGAGACGATTGCCTTTCTGTTGGTGCCCGGCTTTTCTGCCATGGGGTTCATTTCGGCAGTGGAGCCACTGCGGGTGGCCAATCGTTTTGGTGGGCAGT  >  ACB143/358633‑358898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: