| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,386,811 | C→T | 100% | intergenic (‑820/+831) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,811 | 0 | C | T | 100.0% | 128.7 / ‑6.8 | 37 | intergenic (‑820/+831) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/28); minor base G (1/0); total (9/28) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCC > ACB143/5386678‑5386935 | aCGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCg > 2:295704/1‑136 (MQ=255) cTGAGCGTCAGACGCCGTTGAAAAGATAAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCGGCTTGCAAACAAAAAAACAACCGCTACCAGCGGTGGTTTGTTTGGGGGATCAAGAGCTTCCATct > 5:264369/1‑136 (MQ=255) cGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCtttt > 2:283032/1‑136 (MQ=255) cccGTAGAAAAGATAAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAgg < 8:166953/136‑1 (MQ=255) ccGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACACTTTTTCCGAAgg > 5:192700/1‑135 (MQ=255) ccGTAGAAAAGATCAAAGGATCTTCTTGAGAACCTTATTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGATTGTTTGTCGGATAAACAGCAACCAACACTATTTACGAAgg > 3:19548/1‑135 (MQ=255) aGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACt > 7:641/1‑136 (MQ=255) tCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTcag < 5:121818/136‑1 (MQ=255) tCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTcag < 3:241031/136‑1 (MQ=255) aaaGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTcag > 1:27932/1‑134 (MQ=255) ggATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCagag < 1:29701/136‑1 (MQ=255) tcttctTGAGATCCTTTTTTTCTGCGCGTAAGCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 7:257006/135‑1 (MQ=255) cttGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 1:104811/131‑1 (MQ=255) cttGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 5:177696/131‑1 (MQ=255) cttGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 5:277731/131‑1 (MQ=255) cttGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 6:204771/131‑1 (MQ=255) cttGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 2:256737/131‑1 (MQ=255) cttGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 1:33004/131‑1 (MQ=255) ttGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGAAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGAt < 6:169802/135‑1 (MQ=255) gagaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATAc < 7:37004/135‑1 (MQ=255) cTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATa < 4:180444/135‑1 (MQ=255) cTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATa < 3:22849/135‑1 (MQ=255) tttCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttc > 1:116502/1‑136 (MQ=255) tCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttct < 5:223508/135‑1 (MQ=255) ctcgTCATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGTATCAAGAGCTACCAACTCTTTTTCCGCAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAg < 8:150928/134‑1 (MQ=255) cgctTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTa < 3:41765/135‑1 (MQ=255) cgcgTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTa < 7:278723/135‑1 (MQ=255) gTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtgt < 2:179851/136‑1 (MQ=255) tAATCTGAGGCTTGCAAAAAAAACAACCACCGCTACCAGTTGTGGTTTGTTTGTCGTATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTa < 5:35907/136‑1 (MQ=255) ctTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTa < 5:223733/136‑1 (MQ=255) caaacaaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcc < 7:204038/136‑1 (MQ=255) aaaaCCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTc < 3:239596/136‑1 (MQ=255) aaCCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCATATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCaa < 4:189309/136‑1 (MQ=255) ccAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGACCTCTGTAg < 5:304991/136‑1 (MQ=255) ccAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg < 3:250397/136‑1 (MQ=255) gCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc < 1:230090/136‑1 (MQ=255) ggtTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGcc > 4:14935/1‑135 (MQ=255) | ACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCC > ACB143/5386678‑5386935 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |