Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	5,525,438	G→A	5.9%	S178S (AGC→AGT)	sdh ←	Serine 3‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,525,438	0	G	A	5.9%	229.2 / 3.1	68	S178S (AGC→AGT)	sdh	Serine 3‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (33/31);  new base A (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGAAGATGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCACCGACCCGACGTTCAGGA  >  ACB143/5525307‑5525571                                                                                                                                    |                                                                                                                                      cAGAAGATGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTc                                                                                                                                    <  3:236543/135‑1 (MQ=255)  agaagaTGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTcac                                                                                                                                  <  8:83854/136‑1 (MQ=255)  agaagaTGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTcac                                                                                                                                  <  6:52285/136‑1 (MQ=255)  agaagaTGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTcac                                                                                                                                  <  5:216571/136‑1 (MQ=255)  agaagaTGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTcac                                                                                                                                  <  3:176302/136‑1 (MQ=255)  agaagaTGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCAGCTTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTcac                                                                                                                                  <  1:244666/136‑1 (MQ=255)  agaagaTGGGCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGACGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTcac                                                                                                                                  <  4:44060/136‑1 (MQ=255)    aagaTGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCacac                                                                                                                                >  5:210101/1‑136 (MQ=255)     agaTGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCAcacg                                                                                                                               >  1:215081/1‑136 (MQ=255)        tGGTCTCGGCAATATCCTGTGGCTGGAGCGGATCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCGCGAAGCGCACCAGCGAGAACTCGCGCTCGCACAGGCCCGGCTCGATGTTGCTCAcacgca                                                                                                                             <  2:213587/135‑1 (MQ=255)          gTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCACGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCTCacgcacgc                                                                                                                          >  5:44091/1‑136 (MQ=255)            ctcGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCg                                                                                                                        >  7:256931/1‑136 (MQ=255)              cGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACCAACGCCgg                                                                                                                       >  6:293965/1‑135 (MQ=255)              cGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGGCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGt                                                                                                                      <  7:79961/136‑1 (MQ=255)               ggCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCg                                                                                                                        <  3:135312/133‑1 (MQ=255)                 cAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTAcc                                                                                                                   <  3:133122/136‑1 (MQ=255)                 cAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTAcc                                                                                                                   >  4:56480/1‑136 (MQ=255)                   atatCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACcgc                                                                                                                 >  4:154795/1‑136 (MQ=255)                       ccTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAg                                                                                                             >  1:63998/1‑136 (MQ=255)                             gCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCa                                                                                                       <  3:9174/136‑1 (MQ=255)                             gCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCGCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCa                                                                                                       <  6:24650/136‑1 (MQ=255)                              cTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCa                                                                                                              <  2:265156/128‑1 (MQ=255)                              cTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCa                                                                                                              >  1:265156/1‑128 (MQ=255)                              cTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCaa                                                                                                      >  1:88442/1‑136 (MQ=255)                                     ggCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGc                                                                                               >  2:244834/1‑136 (MQ=255)                                                    taggtagCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCa                                                                                                  <  7:206425/118‑1 (MQ=255)                                                    taggtagCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCa                                                                                                  >  8:206425/1‑118 (MQ=255)                                                    taggtagCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAGCTGGc                                                                                >  2:149522/1‑136 (MQ=255)                                                    taggtagCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGc                                                                                >  7:161352/1‑136 (MQ=255)                                                        tagCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCcgac                                                                            <  1:240824/136‑1 (MQ=255)                                                            aTCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAg                                                                        >  8:277419/1‑136 (MQ=255)                                                            aTCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAg                                                                        >  2:83354/1‑136 (MQ=255)                                                             tCGGACTGGGCAGGGTCACCCCCGAAGCGAACAAGCGATAACTCGATCTCGCACAGGCCCGGCTCGATGTTGCGCACACGCACGCCTGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAgg                                                                       <  4:55860/136‑1 (MQ=255)                                                              cGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGc                                                                      >  7:167259/1‑136 (MQ=255)                                                                 aCTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCtt                                                                   >  8:210679/1‑136 (MQ=255)                                                                  cTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTg                                                                  <  4:172985/136‑1 (MQ=255)                                                                  cTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTg                                                                  <  3:172277/136‑1 (MQ=255)                                                                  cTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTg                                                                  <  6:274782/136‑1 (MQ=255)                                                                  cTTGGCCTGGGCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTg                                                                  <  1:241423/136‑1 (MQ=255)                                                                     ggCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTgg                                                                 <  7:181513/134‑1 (MQ=255)                                                                     ggCCTGGGCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTg                                                               <  1:145781/136‑1 (MQ=255)                                                                       ccTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTgcc                                                             <  3:202930/136‑1 (MQ=255)                                                                        cTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTgccg                                                            >  7:258941/1‑136 (MQ=255)                                                                        cTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTgccg                                                            >  3:95501/1‑136 (MQ=255)                                                                         tGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTgccgc                                                           >  3:218367/1‑136 (MQ=255)                                                                            tCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCAt                                                        <  4:252074/136‑1 (MQ=255)                                                                              aCCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCACCGCAGGCTCAGCGAGAACTGTCCGCCGAAGGACTTGGTGCCGCCATa                                                       >  4:121715/1‑135 (MQ=255)                                                                                      aGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTtgct                                              <  6:309886/136‑1 (MQ=255)                                                                                         gcACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGcc                                           <  6:139788/136‑1 (MQ=255)                                                                                             cAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTcac                                                                                                                                  >  6:22660/1‑45 (MQ=255)                                                                                             cAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTcac                                                                                                                                  <  5:22660/45‑1 (MQ=255)                                                                                             cAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCggg                                       >  4:169174/1‑136 (MQ=255)                                                                                                         gCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGtt                            <  5:95962/135‑1 (MQ=255)                                                                                                            ctcGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGccc                        >  3:286595/1‑136 (MQ=255)                                                                                                             tcGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGCCGAAGGCCTTGGTGCCGACATACACGTTGCTGCCCGGGTACGGATAGTTGCACg                       >  5:135461/1‑136 (MQ=255)                                                                                                             tcGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGcc                         <  5:260870/134‑1 (MQ=255)                                                                                                                 acaGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCAc                   >  8:214543/1‑136 (MQ=255)                                                                                                                     gCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCAccgac               <  6:307356/136‑1 (MQ=255)                                                                                                                      cccGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCAccgacc              >  8:293035/1‑136 (MQ=255)                                                                                                                        cGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCAccgccccg            >  1:79212/1‑136 (MQ=255)                                                                                                                        cGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCAccgacccg            >  5:141005/1‑136 (MQ=255)                                                                                                                        cGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCAccgacccg            >  7:32219/1‑136 (MQ=255)                                                                                                                            tCGATGTTACTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTtgct                                              <  3:178614/98‑1 (MQ=255)                                                                                                                            tCGATGTTACTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTtgct                                              <  3:182677/98‑1 (MQ=255)                                                                                                                            tCGATGTTACTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTtgct                                              >  4:182677/1‑98 (MQ=255)                                                                                                                            tCGATGTTACTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTtgct                                              >  4:178614/1‑98 (MQ=255)                                                                                                                             cGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCACCGACCCGACGtt       >  7:122364/1‑136 (MQ=255)                                                                                                                                  ttGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCACCGACCCGACGTTCAGGa  >  2:199642/1‑136 (MQ=255)                                                                                                                                    |                                                                                                                                      CAGAAGATGGTCTCGGCAATATCCTGTGGCTGGATCGGCTCCGCACCCGCGTAGGTAGCATCGTACTTGGCCTGGTCACCCCCGAAGCGCACCAGCGAGAACTCGCTCTCGCACAGGCCCGGCTCGATGTTGCTCACACGCACGCCGGTACCGCGCAGGTCGCAACGCAGGCTCAGCGAGAACTGGCCGACGAAGGCCTTGGTGCCGCCATACACGTTGCTGCCCGGGTACGGATAGTTGCCCGCCACCGACCCGACGTTCAGGA  >  ACB143/5525307‑5525571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑