Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 586,555 A→C 20.8% V212G (GTC→GGC)  ompW ← Outer membrane protein W

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143586,5550AC20.8% 38.5 / 2.5 38V212G (GTC→GGC) ompWOuter membrane protein W
Reads supporting (aligned to +/- strand):  ref base A (11/17);  new base C (5/2);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

GGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCGGTCAGCATGTAGTCCATGCCCAGTT  >  ACB143/586420‑586684
                                                                                                                                       |                                                                                                                                 
ggctggggCATCCGTGCCGCCGCACTGGGCTACGGGCTAGGGCGGCACCGCGCGAGGGCGGCCGAGCCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcga                                                                                                                                   <  3:166159/131‑1 (MQ=255)
 gCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgac                                                                                                                                  <  7:62421/136‑1 (MQ=255)
 gCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcga                                                                                                                                   <  7:45893/135‑1 (MQ=255)
    tGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAATGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgccctcgacgtc                                                                                                                               >  2:264578/1‑136 (MQ=255)
    tGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGCCCATGTATACCCACGGgtggacggcgccggc                                                                                                                               >  7:33835/1‑134 (MQ=255)
    tGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcggcggcggcggg                                                                                                                               >  6:118749/1‑134 (MQ=255)
         aTCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtgggcgtcgccgtgggccc                                                                                                                          >  5:112585/1‑130 (MQ=255)
            agggccgccGCACTGTGCTACTGGCTTTGGCTGCACCGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCGTgg                                                                                                                       <  6:305641/133‑1 (MQ=255)
                   ccgcACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTAGCCCGCGGCGTGGACCATGGTGTTggt                                                                                                                >  7:124159/1‑134 (MQ=255)
                    cgcACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACAGGTCGCCGTCGGCGTCGGCCGTGGGCTTgggg                                                                                                               >  7:49828/1‑133 (MQ=255)
                              tACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGCCCATGTATACCCACGGGTCGCCCGCCCCCTCGGCCGTGGGCTTGCGGTTGCCAAggg                                                                                                     >  6:232170/1‑136 (MQ=255)
                              tACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGGCGACGGCGACCCTGGGCTTGGGGTAGcccacgg                                                                                                     >  8:87748/1‑131 (MQ=255)
                              tACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACCTCGGCGTCGCCCTTGGTGTTGCTGTTGGCAAggg                                                                                                     >  5:116436/1‑136 (MQ=255)
                                   gCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCCCGGGTCCGCGTCGACCTGGACCATGGGCTTTGGGGATCCAAGGGCgggc                                                                                                >  8:213377/1‑134 (MQ=255)
                                   gCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGCCGTCGACCATTGTGTTGATGTAGCCAACGGCGGTc                                                                                                >  5:299495/1‑136 (MQ=255)
                                      aTGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGCCGGCGACGTCGACCTTGGTATTGCTGTAGCCCAGGGCgggaggg                                                                                             >  2:191637/1‑131 (MQ=255)
                                        ggCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGCCCGCGCCCGAGCCCATGGTGTTGGGGTAGCCCAGGGGGGGCGGGcc                                                                                           >  8:142688/1‑136 (MQ=255)
                                                cgcgcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGCCCGCGCCGGCGACCGTTGTCTTGGTGTATCCCAGGGCGGTCGGGGCGGTGACGc                                                                                   >  3:102326/1‑136 (MQ=255)
                                                cgcgcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGCCGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGc                                                                                   >  7:181769/1‑136 (MQ=255)
                                                             cggcggCCGCCCTGCAATGTTCTGGGCACAACTTGTAGCCACCACCGACCATGTATCCCCCCGGGGCGACGGCGACGGCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtc                                                                      <  7:142688/132‑1 (MQ=255)
                                                                 ggCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatg                                                                  <  1:22839/136‑1 (MQ=255)
                                                                 ggCCGCCCTGAAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCAAGTATACCCACGGGTCGACGTCGACGTAGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatg                                                                  <  4:51944/136‑1 (MQ=255)
                                                                  gccgccCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGCCGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatg                                                                  <  8:181769/135‑1 (MQ=255)
                                                                  gccgccCGTCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatg                                                                  <  2:101354/135‑1 (MQ=255)
                                                                  gcccccCTGCATTGTTCATTTCAGAACTTGTAGACAAGACCGACCATGTATACCCACGGGCCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgt                                                                 <  5:213982/136‑1 (MQ=255)
                                                                  ccggaccTGCAATTTTCAGGACAGATCTTGTAGCGATGACCGACGAGTTATACCACCGGCTGTAGGTCGAGTTCGACCTTGTTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgt                                                                 <  6:177069/131‑1 (MQ=255)
                                                                       ccggaaaTGTTCAGGTCAGAACTTTTAGCCAAGACCTACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACg                                                            <  8:55829/131‑1 (MQ=255)
                                                                         tGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGGTGTAGCCCAGGGCGGTCGGGCCGTTGACGCTGGGCGTGGGGTCGATGTCGACGtt                                                          >  2:302773/1‑135 (MQ=255)
                                                                                   aGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCGTGGGGGTTCTGTAGCCAAGGGCGGTCGGGCCGGTGACGCTGGCCTTGGTGTCGATGTGGACGTACCAGACCgcg                                                >  6:105458/1‑134 (MQ=255)
                                                                                   aGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTAGACGTCGACCATGGTCTTTCTGTAGCCAAGGGCGGTCCGGCCCTTGACGCTGGCCGTGGTGTCGATGTCGACGTACCACCCCGAg                                                >  8:123757/1‑136 (MQ=255)
                                                                                           ccTTGTAGCCAAGCCCGAACATTTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTAc                                        <  5:232170/135‑1 (MQ=255)
                                                                                           aCTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGCCGTCGACGTCGCCCGTGGTCGTGCTGTAGCCAAGGGCGGGCGGGCCGTTGACGCTGGCCGTGGTGTCGATGTCGACGTTCCAGCCCGAGGCGttttc                                        >  3:153128/1‑134 (MQ=255)
                                                                                                  gCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTGTTTGTGTAGCCAAGGGCGGTCGGGGCGGTGACGCTGGCCTTGGTGTCGATGTGGACGTACCAGACCGAGGCGTTTAACAGCAgg                                 >  2:10493/1‑136 (MQ=255)
                                                                                                  gCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAgg                                 >  4:234316/1‑136 (MQ=255)
                                                                                                  gCCAAGACCGACCATGTATACCCACGGGTCGACGTCGAAGTCGACCGTGGTCTTTGTGTAGCCAAGGGCGGTCGGGCCCGTTTCGCTGGGCTTGGTGTCGCTGTGGACGGAACAGACCGAGGCGTTTTACAGCAgg                                 >  4:85220/1‑136 (MQ=255)
                                                                                                       gaccgtccAGGTATACCCACGGGTCGAGGTGGACGTGGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTc                            <  1:303974/136‑1 (MQ=255)
                                                                                                       gaccgaccATGTTTACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTc                            <  4:156381/136‑1 (MQ=255)
                                                                                                       gaccgaccATGATTACCCACGGGTCGACGTCGACGTCGACCGTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTc                            <  6:176716/136‑1 (MQ=255)
                                                                                                        accgagcATGTATACCCTCTGGTCGACGTCGAGGGCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCg                           <  5:262051/136‑1 (MQ=255)
                                                                                                         ccgaccATGTATCCCCACGGGGCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCgg                          <  1:98595/136‑1 (MQ=255)
                                                                                                           gaccATGTATACCCACGGGTCGACGTCGACGGCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCGGTc                        <  2:115211/136‑1 (MQ=255)
                                                                                                              cATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCGGTCAGc                     <  5:67635/136‑1 (MQ=255)
                                                                                                              aaTGTATCCCCACGGGGCGACGCCGCCGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCGGTCAGc                     <  5:9134/135‑1 (MQ=255)
                                                                                                               aTGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCGGTCAGCa                    <  1:264578/136‑1 (MQ=255)
                                                                                                               aTGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGGTTACCACCAGGTTGTCGGTCAGCa                    >  7:299391/1‑136 (MQ=255)
                                                                                                                             gtcgacgtcgacgtcgacCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCGGTCAGCATGTAGTCCATGccc      >  2:113531/1‑136 (MQ=255)
                                                                                                                             gtcgacgtcgacgtcgacCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCGGTCAGCATGTAGTCCATGcc       >  1:39150/1‑135 (MQ=255)
                                                                                                                             gtagacgtcgacgtcgacCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGCCCGAGGCGGTTACCAGCCGGTTGTCGGTCAGCATGTAGTGCATGcc       >  8:97831/4‑135 (MQ=255)
                                                                                                                              tcgacgtcgacgtcgacCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGGTTACCAGCAGGTTGTCGGTCAGCATGTAGTACCTGcc       >  2:67877/1‑134 (MQ=255)
                                                                                                                               cgacgtcgacgtcgacCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGCTGTCGACGTACCCGACCGAGGCGTGTTCCAGCAGGTTGTCGGTCCGCATGTAGTCCCTGccccg    >  4:35986/1‑134 (MQ=255)
                                                                                                                                 acggcgacgtcgacCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCGGTCAGCATGTAGTCCATGCCCAGtt  <  6:197468/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                 
GGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCGACGTACCAGACCGAGGCGTTTACCAGCAGGTTGTCGGTCAGCATGTAGTCCATGCCCAGTT  >  ACB143/586420‑586684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑