Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 6,120,698 G→A 5.6% A35V (GCG→GTG)  gbsA ← Betaine aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1436,120,6980GA 5.6% 247.2 / 2.1 71A35V (GCG→GTG) gbsABetaine aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (36/31);  new base A (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGGCAACCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGT  >  ACB143/6120568‑6120831
                                                                                                                                  |                                                                                                                                     
cgcgGCAACCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGc                                                                                                                                      <  3:11391/132‑1 (MQ=255)
    gaaaCCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCGGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGt                                                                                                                              <  5:139727/134‑1 (MQ=255)
    gCAACCTCACGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGt                                                                                                                              <  6:311398/136‑1 (MQ=255)
    gCAACCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCATAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGt                                                                                                                              <  4:104643/136‑1 (MQ=255)
    gCAACCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGt                                                                                                                              <  3:105599/136‑1 (MQ=255)
    gCAACCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGt                                                                                                                              <  5:270689/136‑1 (MQ=255)
    gCAACCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGt                                                                                                                              <  5:31848/136‑1 (MQ=255)
    gCAACCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGt                                                                                                                              <  3:275720/136‑1 (MQ=255)
    gCAACCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTGCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGt                                                                                                                              <  5:155394/136‑1 (MQ=255)
        ccTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCgt                                                                                                                          >  8:243832/1‑136 (MQ=255)
            aaGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCgtggtg                                                                                                                      <  4:11536/136‑1 (MQ=255)
            aaGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCgtggt                                                                                                                       <  3:166657/135‑1 (MQ=255)
            aaGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCgtggt                                                                                                                       <  4:306999/135‑1 (MQ=255)
            aaGCAACCAGGGCACCCGATGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGTGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCgtggtg                                                                                                                      <  8:111646/136‑1 (MQ=255)
                   cAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGGGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGt                                                                                                               <  3:152238/136‑1 (MQ=255)
                      gTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTtgt                                                                                                            >  2:280750/1‑136 (MQ=255)
                      gTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGGCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTtgt                                                                                                            <  2:317759/136‑1 (MQ=255)
                        cACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTtgtg                                                                                                           >  6:270531/1‑135 (MQ=255)
                           ccGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATa                                                                                                       <  5:112323/136‑1 (MQ=255)
                           ccGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATa                                                                                                       <  3:56309/136‑1 (MQ=255)
                             gCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACt                                                                                                     <  6:200108/136‑1 (MQ=255)
                                 cgcAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAg                                                                                                  <  7:246568/135‑1 (MQ=255)
                                   cAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGtg                                                                                               >  4:223307/1‑136 (MQ=255)
                                     ggACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGtgct                                                                                             <  5:223095/136‑1 (MQ=255)
                                              cGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGt                                                                                    >  5:206066/1‑136 (MQ=255)
                                              cGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGt                                                                                    >  6:259739/1‑136 (MQ=255)
                                              cGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGt                                                                                    >  6:251699/1‑136 (MQ=255)
                                              cGGTTCAGAGCGTTGCTGACAGCACAGCGCCGAGCCGCCTGCAGGGCGCAGTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGt                                                                                    >  7:105350/1‑136 (MQ=255)
                                                   cAGAGCGTTGCTGTCAGCACAGCGCCGAGTCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTc                                                                               >  8:40850/1‑136 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGcc                                                                                                                    <  6:269471/88‑1 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGcc                                                                                                                    >  5:269471/1‑88 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATAGTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    >  3:68420/1‑136 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    >  6:265147/1‑136 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    >  2:8699/1‑136 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    >  6:70868/1‑136 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    >  1:313783/1‑136 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    >  2:119892/1‑136 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    >  3:216550/1‑136 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    >  2:181239/1‑136 (MQ=255)
                                                              tGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCAGTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCCCAAGGTCCCTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTgcacgac                                                                    >  7:125421/1‑130 (MQ=255)
                                                                tCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    <  2:177801/134‑1 (MQ=255)
                                                                tCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTc                                                                    <  4:216550/134‑1 (MQ=255)
                                                                               gccgccTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGt                                                   <  5:134694/136‑1 (MQ=255)
                                                                                 cgccTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGc                                                 >  3:233673/1‑136 (MQ=255)
                                                                                 cgccTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCACCACAAGGTCCGTGGTGcc                                                                                                                    >  2:35900/1‑69 (MQ=255)
                                                                                 cgccTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCACCACAAGGTCCGTGGTGcc                                                                                                                    >  2:31350/1‑69 (MQ=255)
                                                                                 cgccTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCACCACAAGGTCCGTGGTGcc                                                                                                                    <  1:35900/69‑1 (MQ=255)
                                                                                 cgccTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCACCACAAGGTCCGTGGTGcc                                                                                                                    <  1:31350/69‑1 (MQ=255)
                                                                                  gccTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCg                                                <  2:27780/136‑1 (MQ=255)
                                                                                      gCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCAAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTTGCCGTCGATGCAGGTTAGCCAGTGCGGAGc                                            >  3:189957/1‑136 (MQ=255)
                                                                                       cAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGc                                            <  3:2689/135‑1 (MQ=255)
                                                                                       cAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGc                                            <  1:205083/135‑1 (MQ=255)
                                                                                       cAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCt                                           <  1:22405/136‑1 (MQ=255)
                                                                                                     cGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCatgat                             <  2:202641/136‑1 (MQ=255)
                                                                                                                   gCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGgc                <  1:280750/135‑1 (MQ=255)
                                                                                                                      tGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGt            >  6:141517/1‑136 (MQ=255)
                                                                                                                       gggCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGtt           >  8:262722/1‑136 (MQ=255)
                                                                                                                        ggCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAATGTTAGCCAGTGCGGAGCTATAGGATGCATGATGATCACCTCAGGCGCGtt           >  3:219348/1‑135 (MQ=255)
                                                                                                                           gATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAg        >  8:61223/1‑135 (MQ=255)
                                                                                                                           gATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAg        >  3:45282/1‑135 (MQ=255)
                                                                                                                           gATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAg        >  3:150514/1‑135 (MQ=255)
                                                                                                                           gATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAg        >  3:122982/1‑135 (MQ=255)
                                                                                                                            aTGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGca      >  1:308286/1‑136 (MQ=255)
                                                                                                                               gTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAg   <  7:105648/136‑1 (MQ=255)
                                                                                                                                tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATTATCACCTCAGGCGCGTTGAGCACAGt  >  3:259941/1‑136 (MQ=255)
                                                                                                                                tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAg   >  4:217447/1‑135 (MQ=255)
                                                                                                                                tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAg   >  4:119079/1‑135 (MQ=255)
                                                                                                                                tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGt  >  5:57557/1‑136 (MQ=255)
                                                                                                                                tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGt  >  1:29923/1‑136 (MQ=255)
                                                                                                                                tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGt  >  1:273655/1‑136 (MQ=255)
                                                                                                                                tCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGt  >  1:207657/1‑136 (MQ=255)
                                                                                                                                  |                                                                                                                                     
CGCGGCAACCTCAAGCAACCAGGTCACCCGCTGCGCAGGACGCACACGGTTCAGAGCGTTGCTGTCAGCACAGCGCCGAGCCGCCTGCAGGGCGCATTCGGCGTCGGCCACACTGGCTTGGGCGATGGTCGCCACAAGGTCCGTGGTGCCAGGGTTGTGGATACTCAGGTGCTGCTCACTGTCGGTCCACTGGCCGTCGATGAAGTTTAGCCAGTGCGGAGCTATCGGATGCATGATGATCACCTCAGGCGCGTTGAGCACAGT  >  ACB143/6120568‑6120831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑