breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 44,301 (T)9→8 intergenic (+132/‑359) oprD_1 → / → czcR_2 Porin D/Transcriptional activator protein CzcR
RA 120,983 (T)9→8 intergenic (+280/+92) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB
RA 179,484 T→C C316R (TGC→CGC)  CJ019_00182 → hypothetical protein
RA 690,144 A→G intergenic (+21/‑2438) bauC → / → PP_RS08750 Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
JC 2,191,046 +CTGAAAATATCTCTTAT intergenic (‑1/‑313) PP_RS10045 ← / → purU_3 LysR family transcriptional regulator/Formyltetrahydrofolate deformylase
JC 4,257,335 +GCGGCCGC intergenic (‑281/+7) CJ019_03991 ← / ← CJ019_03994 putative ABC transporter ATP‑binding protein/hypothetical protein
RA 4,759,606 G→A A28T (GCC→ACC)  ligM_KToptimized → sequence generated by OPTIMIZER for KT2440
RA 5,386,500 C→A intergenic (‑509/+1142) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,503 C→G intergenic (‑512/+1139) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,506 1 bp→GT intergenic (‑515/+1136) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,507 +C intergenic (‑516/+1135) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,510 2 bp→TA intergenic (‑519/+1131) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,513 +AA intergenic (‑522/+1129) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,514 T→C intergenic (‑523/+1128) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,517 G→C intergenic (‑526/+1125) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,519 G→A intergenic (‑528/+1123) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
RA 5,386,811 C→T intergenic (‑820/+831) CJ019_05070 ← / ← nptII hypothetical protein/neomycin phosphotransferase
JC 5,391,707 +54 bp intergenic (+124/‑1253) ligH → / → CJ019_05072 10‑formyltetrahydrofolate synthetase/hypothetical protein
RA 5,760,598 (A)6→5 coding (781/834 nt) CJ019_05400 → hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ ACB143 87933 88060 128 21 [19] [18] 21 [CJ019_00094]–[CJ019_00095] [CJ019_00094],[CJ019_00095]
* * ÷ ACB143 277582 277681 100 21 [18] [19] 20 [betT2]–[tauD_2] [betT2],[tauD_2]
* * ÷ ACB143 517190 517314 125 28 [15] [19] 20 tyrS/birA Tyrosine‑‑tRNA ligase/Bifunctional ligase/repressor BirA
* * ÷ ACB143 607303 607432 130 20 [19] [19] 21 [ispA] [ispA]
* * ÷ ACB143 674228 674418 191 24 [17] [19] 20 [nimR_1]–[mcpH_2] [nimR_1],[mcpH_2]
* * ÷ ACB143 1499936 1500088 153 23 [19] [19] 23 [sspA] [sspA]
* * ÷ ACB143 1608696 1608814 119 21 [18] [19] 20 [CJ019_01513]–[CJ019_01514] [CJ019_01513],[CJ019_01514]
* * ÷ ACB143 1697643 1697759 117 22 [17] [19] 22 [CJ019_01593]–[CJ019_01594] [CJ019_01593],[CJ019_01594]
* * ÷ ACB143 1914554 1914659 106 21 [19] [19] 20 [ppsC_1]–[CJ019_01830] [ppsC_1],[CJ019_01830]
* * ÷ ACB143 1940799 1940925 127 21 [14] [18] 20 CJ019_01857/gshAB hypothetical protein/Glutathione biosynthesis bifunctional proteinGshAB
* * ÷ ACB143 2288809 2288922 114 20 [18] [19] 21 CJ019_02171/CJ019_02172 hypothetical protein/hypothetical protein
* * ÷ ACB143 2467190 2467208 19 26 [1] [14] 36 CJ019_02325/tal hypothetical protein/Transaldolase
* * ÷ ACB143 2657037 2657151 115 20 [19] [19] 20 prpC/acnM 2‑methylcitrate synthase/Aconitate hydratase A
* * ÷ ACB143 2716442 2716446 5 38 [0] [0] 45 rhaR_3 HTH‑type transcriptional activator RhaR
* * ÷ ACB143 3046179 3046300 122 20 [19] [19] 23 [rcsC_5]–[degU_2] [rcsC_5],[degU_2]
* * ÷ ACB143 3769361 3769492 132 21 [19] [18] 20 CJ019_03581/CJ019_03582 hypothetical protein/hypothetical protein
* * ÷ ACB143 4331603 4331778 176 20 [19] [19] 20 [ribBA_2] [ribBA_2]
* * ÷ ACB143 4521548 4521686 139 22 [19] [18] 21 [CJ019_04289] [CJ019_04289]
* * ÷ ACB143 4732379 4732484 106 20 [19] [19] 20 [CJ019_04468] [CJ019_04468]
* * ÷ ACB143 4944211 4944340 130 20 [19] [18] 20 [CJ019_04642] [CJ019_04642]
* * ÷ ACB143 5250107 5250228 122 20 [19] [16] 21 [CJ019_04947]–[CJ019_04948] [CJ019_04947],[CJ019_04948]
* * ÷ ACB143 5503642 5503834 193 20 [19] [19] 20 [CJ019_05176] [CJ019_05176]
* * ÷ ACB143 5678536 5678659 124 20 [19] [19] 20 CJ019_05328/CJ019_05329 hypothetical protein/hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 1837190 =0 (0.000)54 (0.940) 32/252 0.4 34.8% coding (660/2754 nt) barA Signal transduction histidine‑protein kinaseBarA
?ACB143 = 2172433 202 (3.500)intergenic (+175/+93) CJ019_02060/CJ019_02061 IS1182 family transposase ISPpu16/hypothetical protein
* ? ACB143 = 18371930 (0.000)33 (0.570) 24/254 0.9 35.3% coding (657/2754 nt) barA Signal transduction histidine‑protein kinaseBarA
?ACB143 2170759 = 121 (2.080)intergenic (‑774/‑63) arsR1/CJ019_02060 Arsenic resistance transcriptional regulatorArsR1/IS1182 family transposase ISPpu16
* ? ACB143 2155685 =49 (0.820)139 (2.320) 53/262 0.0 73.4% coding (301/369 nt) tmk_1 Thymidylate kinase
?ACB143 = 2220562 52 (0.870)coding (36/369 nt) tmk_2 Thymidylate kinase
* ? ACB143 2170755 =130 (2.170)43 (0.740) 30/254 0.5 40.6% intergenic (‑770/‑67) arsR1/CJ019_02060 Arsenic resistance transcriptional regulatorArsR1/IS1182 family transposase ISPpu16
?ACB143 2716447 = 0 (0.000)coding (185/846 nt) rhaR_3 HTH‑type transcriptional activator RhaR
* ? ACB143 2170758 =122 (2.030)33 (0.550) 24/260 1.0 34.9% intergenic (‑773/‑64) arsR1/CJ019_02060 Arsenic resistance transcriptional regulatorArsR1/IS1182 family transposase ISPpu16
?ACB143 5869157 = 2 (0.030)coding (690/2280 nt) ptsP Phosphoenolpyruvate‑dependent phosphotransferasesystem
* ? ACB143 = 2172433202 (3.370)81 (1.350) 38/262 0.2 44.3% intergenic (+175/+93) CJ019_02060/CJ019_02061 IS1182 family transposase ISPpu16/hypothetical protein
?ACB143 = 5869159 2 (0.030)coding (688/2280 nt) ptsP Phosphoenolpyruvate‑dependent phosphotransferasesystem
* ? ACB143 = 2172438207 (3.450)38 (0.660) 23/252 1.0 27.6% intergenic (+180/+88) CJ019_02060/CJ019_02061 IS1182 family transposase ISPpu16/hypothetical protein
?ACB143 = 2716441 0 (0.000)coding (191/846 nt) rhaR_3 HTH‑type transcriptional activator RhaR
* ? ACB143 3359593 =47 (0.780)107 (1.780) 51/262 0.0 69.0% intergenic (+219/‑151) pikAII_1/CJ019_03160 Narbonolide/10‑deoxymethynolide synthase PikA2,modules 3 and 4/Tn3 family transposase ISPa42
?ACB143 4755885 = 49 (0.820)coding (684/1659 nt) yojI ABC transporter ATP‑binding/permease proteinYojI
* ? ACB143 = 337660555 (0.920)127 (2.120) 60/262 0.0 65.8% coding (501/546 nt) CJ019_03184 hypothetical protein
?ACB143 = 4765778 77 (1.280)coding (6629/10413 nt) grsB Gramicidin S synthase 2