Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143303,9940GT30.2% 70.3 / 12.4 53G477G (GGC→GGA) ydeP_1Protein YdeP
Reads supporting (aligned to +/- strand):  ref base G (21/16);  new base T (16/0);  total (37/16)
Fisher's exact test for biased strand distribution p-value = 1.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGGATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATGGCCATTGCGCCGCGGCACAGGGAAGCCAAAGCGCTTTTCGATGGCATCCAGCAGCGCCTCTGGCGG  >  ACB143/303865‑304128
                                                                                                                                   |                                                                                                                                       
cAGGATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGccc                                                                                                                                   <  4:138679/136‑1 (MQ=255)
 aGGATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGGACTTGGGGGAGT‑TTgccgcc                                                                                                                                    >  2:371412/1‑135 (MQ=255)
 aGGATCAGTGCCGGCGTGCCATGGACCAGGTGGCTACGGTTGATCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCa                                                                                                                                  <  8:156631/136‑1 (MQ=255)
  ggATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCAGGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTTGGCGAAG‑TTGGCGcccca                                                                                                                                 >  8:79285/1‑134 (MQ=255)
  ggATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTTGGGGAAG‑TTGCCGCCCac                                                                                                                                 >  4:290152/1‑135 (MQ=255)
  ggATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGGGAAG‑TTGCCGCCCaa                                                                                                                                 >  8:210584/1‑136 (MQ=255)
  ggATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCaa                                                                                                                                 >  8:79283/1‑136 (MQ=255)
  ggATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCaa                                                                                                                                 >  4:255695/1‑136 (MQ=255)
  ggATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CGTGGGCGGAG‑TTTCCGCCCaa                                                                                                                                 >  2:4447/1‑136 (MQ=255)
  ggATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CATGTGCGAAA‑TTTGCGCCCaa                                                                                                                                 >  2:365653/1‑136 (MQ=255)
   gATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTTGGCGAAG‑TTGCCGCCCAAg                                                                                                                                >  1:244932/1‑136 (MQ=255)
       aGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑GTTGGGGGGAG‑TTTCCGCCCCAGCCg                                                                                                                             >  2:355216/1‑135 (MQ=255)
       aGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑GGTGGGGGGAG‑TTTGCGCCCCAGccca                                                                                                                            >  3:341131/1‑134 (MQ=255)
       aGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGa                                                                                                                            >  2:73647/1‑136 (MQ=255)
       aGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAACCCgg                                                                                                                            >  6:74751/1‑135 (MQ=255)
           ccTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGGGAAG‑TTGGCGCCCAAGCCGATgt                                                                                                                         >  7:349369/1‑134 (MQ=255)
            cTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGCAC‑TTGCCGCCCAAGCCCCTG‑aa                                                                                                                       >  2:12985/1‑135 (MQ=255)
                ttGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑GTTTGGCGGAG‑TTTCCGCCCCAGCCGATT‑CAAAcc                                                                                                                   >  2:360489/1‑135 (MQ=255)
                 tGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CGTTGGCGAAG‑TTTCCCCCCAAGCCCAAG‑AAAAccat                                                                                                                 >  3:303929/1‑134 (MQ=255)
                     aTGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGcc                                                                                                             <  1:222054/136‑1 (MQ=255)
                             ggTGGCTACGGGTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGGGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATc                                                                                                     <  2:146276/136‑1 (MQ=255)
                                ggCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAg                                                                                                  <  6:1842/136‑1 (MQ=255)
                                ggCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CGTGGGCGAAG‑TTGCCGCCCCAGCCGATG‑AAAAACGTGGCCCGGCCATCgcg                                                                                                  >  8:319839/1‑134 (MQ=255)
                                 gCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTTGGCGAAT‑TTTCCCCCCAAGCCGATG‑AAAACCATGGCCCGGCCATCGAg                                                                                                  >  5:121993/1‑135 (MQ=255)
                                      ggTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTTGGCGAAG‑TTGCCGCCCAAGCCGCTG‑AAAACCTTTGCCCGGCCATCGCGCATAGc                                                                                            >  8:12226/1‑136 (MQ=255)
                                              ttGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑GTTGGGCGAAG‑TTTCCGCCCAAGCCGATG‑AAAACCTTGGGCCGGCCCTCGCGCATCGCATGAATgg                                                                                    >  1:300142/1‑136 (MQ=255)
                                              ttGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATgg                                                                                    <  2:358545/136‑1 (MQ=255)
                                                 gTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCtt                                                                                 <  3:255695/136‑1 (MQ=255)
                                                 gTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCtt                                                                                 <  5:172073/136‑1 (MQ=255)
                                                    cTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCg                                                                               <  6:335742/135‑1 (MQ=255)
                                                    cTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCg                                                                               <  5:271635/135‑1 (MQ=255)
                                                          tgCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTTGGCGAAG‑TTGGCGCCCCAGCCGATT‑AAAACCATGGCCCCGCCCTAGAGCATCGCATGAATTGGTTGGACGgggt                                                                        >  4:111833/1‑136 (MQ=255)
                                                          tgCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGGGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATAGCATGAATGGCTTCGACGgtgt                                                                        >  1:301313/1‑136 (MQ=255)
                                                               ggTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTTCCGCCCCAGCCGATG‑AAAACCTTGGCCCGGCCATCGCGCCTCGCATGAATGGCTTCGACGGTGTTTTgg                                                                   >  5:280097/1‑136 (MQ=255)
                                                               ggTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTTCCGCCCCAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATAGCATGAATGGGGTCGCCGGGGTTTTgg                                                                   >  1:109539/1‑136 (MQ=255)
                                                               ggTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATgg                                                                   <  5:278818/136‑1 (MQ=255)
                                                                 tCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCAt                                                                                          >  7:296423/1‑111 (MQ=255)
                                                                 tCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGACGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCAt                                                                                          <  8:296423/111‑1 (MQ=255)
                                                                  cAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATGGCCa                                                                <  6:239112/136‑1 (MQ=255)
                                                                    gCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTTCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTGGACGGGGTTTTGGCCCtt                                                              >  2:59812/1‑136 (MQ=255)
                                                                    gCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTT‑GGCGAAG‑TTTCCGCCCAAGCCGATG‑AAAACCATGGCCCGGCCCTCGAGCATCGCATGAATGGCTTCGACGGTGGTTTGGCCATTg                                                             >  8:14513/1‑136 (MQ=255)
                                                                    gCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CGTGGGGGAAG‑TTTCCGCCCCACCCGATG‑AAAACCATGGCCCGGCCATCGAGCCTCGCATGAATGGGGTGGCCGGTGTTTTGGGCAtt                                                              >  4:186383/1‑136 (MQ=255)
                                                                       cGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGGGGAG‑TTGCCGCCCCAGCCGATG‑AAAACCGTGGCCCGGCCCTCGAGCATCGCATGAATGGGTTCGACGGTGTTATGGCCCTTgcg                                                           >  3:235289/1‑136 (MQ=255)
                                                                       cGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGGGAAGTTTGCCCCCCAA‑CCGCTG‑AAAACCATTGCCCCGCCAAGGAGCATAGCGAGTAAGGGGTGGGCGGGGTTTTGGCCAtttgg                                                           >  4:81230/1‑133 (MQ=255)
                                                                        gCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATGGCCATTgcg                                                           <  7:12184/135‑1 (MQ=255)
                                                                                       cTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTTGCGCCCCAGCCGCTGAAAAACGTTGGCCC‑GCCCTCGAGCCTCGCCTGGATGGGTTGGACGGTGGTTTGGGCCTTGCGCCGCGGCACCGGGGAg                                           >  8:209721/1‑136 (MQ=255)
                                                                                       cTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTTCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCCTCGAGCATCGCATGAATGGCTTCGACGGGGTTTTGGCCATTTCGCCGCGGCCCAGGGTAg                                           >  1:4668/1‑136 (MQ=255)
                                                                                       cTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATGGCCATTGCGCCGCGGCACAGGGAAg                                           <  1:151358/136‑1 (MQ=255)
                                                                                                  gCTCGGTGTCGGGTGTGG‑CTTTGGCGAAG‑TTGCCCCCCCAGCCCATG‑AAAACCATTGCCCGGCCAACGAGCATCGCATGAAATGCTTAGACCGTGTTATGGCCATTGCGCCCCGGCCCACGGGAGCCAAAgcgcgt                                >  8:102186/1‑134 (MQ=255)
                                                                                                         gtCGGGTGTGG‑CTTGGGCGAAG‑TTTCCGCCCAAGCCGATG‑AAAACCTTTGCCCGGCCATAGAGCATCGCATGAATTGCTTCGACGGTGTTTTGGGCATTGCGCCGCGGCACAGGGAAGCCAAAGCGCTTTTAGAtt                         >  2:14186/1‑135 (MQ=255)
                                                                                                                            gAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATGGCCATTGCGCCGCGGCACAGGGAAGCCAAAGCGCTTTTCGATGGCATCCAGCAGCGCctct       >  8:122659/1‑136 (MQ=255)
                                                                                                                            gAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGGGTTATGGCCATTGCGCCGCGGCACAGGGAAGCCAAAGCGCTTTTCGATGGCATCCAGCAGCGCctct       >  1:12223/1‑136 (MQ=255)
                                                                                                                             aaG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATGGCCATTGCGCCGCGGCACAGGGAAGCCAAAGCGCTTTTCGATGGCATCCAGCAGCGCCTCTg      <  7:210584/136‑1 (MQ=255)
                                                                                                                                  tGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATGGCCATTGCGCCGCGGCACAGGGAAGCCAAAGCGCTTTTCGATGGCATCCAGCAGCGCCTCTggcgg  >  5:324104/1‑136 (MQ=255)
                                                                                                                                   gccgccCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATGGCCATTGCGCCGCGGCACAGGGAAGCCAAAGCGCTTTTCGATGGCATCCAGCAGCGCCTCTggcgg  <  7:122659/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
CAGGATCAGTGCCTGCTTGCCATGGACCAGGTGGCTACGGTTGAGCTTGGTGCTGATGTGCACGGTCAGCTCGCAGTTGCGCAGCGCCTGGGCGGTACGCTCGGTGTCGGGTGTGG‑CTTGGGCGAAG‑TTGCCGCCCAAGCCGATG‑AAAACCTTGGCCCGGCCATCGAGCATCGCATGAATGGCTTCGACGGTGTTATGGCCATTGCGCCGCGGCACAGGGAAGCCAAAGCGCTTTTCGATGGCATCCAGCAGCGCCTCTGGCGG  >  ACB143/303865‑304128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑