| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 690,139 | 1 | . | G | 41.7% | ‑3.2 / 21.1 | 12 | intergenic (+16/‑2443) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base . (6/1); new base G (5/0); total (11/1) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCATGCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGG‑C‑CTC‑CCCGC‑‑TTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGT > ACB143/690009‑690232 | gCATGCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑ggggg > 8:121509/1‑135 (MQ=255) aTGCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑Ggggggg > 8:224193/1‑136 (MQ=255) gCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCgggcgac > 6:254952/1‑130 (MQ=255) gCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGAGG‑c > 2:206715/1‑136 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCCTCGGC‑C‑CTc‑ccccc > 7:117388/1‑134 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGCGGG‑C‑CGC‑CCCCC‑‑GGTGTTTTTGGGggtgggggt > 1:292313/1‑133 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGCAGGCC‑CCC‑CCCGG‑‑TGTTTTTTGGGGgggtggtg > 5:149823/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCGG‑C‑CGCACCCGC‑‑GTTGTTTTTGGGggttgttgg > 6:301282/1‑132 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCCGG‑G‑CGC‑CCCCCCGTTTTTTTTTGGGggtggttt > 4:177286/1‑131 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGGCGGG‑C‑GGC‑CCCCC‑‑TTGGTTTTGTGGggtgggttttg > 5:70865/1‑133 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGAAGC‑C‑CGC‑CCCCC‑‑ATGTTTTTTGGGggttggttttg > 1:308835/1‑133 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGCGGC‑CACCC‑CCCGG‑‑TGTGTTTTTGGGGG‑TGGTGGTGGGTGTTGgggggggta > 8:192147/1‑133 (MQ=255) tCGCAGTTTGCATTCCCTAGCCTGTACGGTAAGTAGATGACGC‑GGGGAGGTCGTCAGG‑C‑CTC‑CCCGC‑‑TTTGTTTTTGGGGGGTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGAtgt < 5:9116/136‑1 (MQ=37) | GCATGCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGG‑C‑CTC‑CCCGC‑‑TTTGTTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGT > ACB143/690009‑690232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |