Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,770,0750GC33.3% 86.6 / 11.0 51A157G (GCG→GGG) CJ019_02613hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (11/23);  new base C (17/0);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.93e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACG  >  ACB143/2769944‑2770210
                                                                                                                                   |                                                                                                                                       
tCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCTGCCGCCACCGGGCGCGCCCCCCCCCCAAccccccccc                                                                                                                                     >  3:137274/1‑135 (MQ=255)
  ggTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAgg                                                                                                                                   <  3:359566/136‑1 (MQ=255)
    tCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAggcg                                                                                                                                 <  3:227809/136‑1 (MQ=255)
     cAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCGCCCCCCCCCCCACACCCCCCAggcgg                                                                                                                                >  7:246374/1‑136 (MQ=255)
     cAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCCCAACCGCCCCCGTGCCTGCAGCCACCCGGCGCACCCCACCCCCCACACCCCCCCgccgg                                                                                                                                >  2:33798/1‑136 (MQ=255)
       aGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGcc                                                                                                                              <  2:75886/136‑1 (MQ=255)
                 cAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCCCCCCCAACACCGCCCAGGCGGCCCAGCTCgg                                                                                                                      >  1:289941/1‑134 (MQ=255)
                 cAGGCCAATACTACAGCGCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGGGCCTGCAGCCACCCGGCGCTCCCCCCCCCCCACACCCCCCCCGCGGCCCAGCTAGGCa                                                                                                                    >  1:42425/1‑136 (MQ=255)
                     ccAATACTGCAGCCCATGCCGATATCAAAAAAACACCCGACAACACTTCGTTGAACACCCGCAACCGCCCCCGTGCTTGCAGCCACCGGGCGCTCCACACCCACCACCCCGCCCAGGCGGCCATGCAGGGCAGAGc                                                                                                                >  1:78734/1‑136 (MQ=255)
                           cTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGGCGCGCCCCCCCCCCAACCCCCCCCACGCGGCCATGCTCGGCAGAGCGATCAg                                                                                                          >  1:338717/1‑136 (MQ=255)
                            tGCAGCCCATGCCGATATCAACAAAACCCCCGCCCACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCTGCCGCCACCGGGCGCGCCCCCCCCCCAACCCCCCCCCGGCGGCCATGCTCGGCAGCGCGCTCAGc                                                                                                         >  3:268602/1‑136 (MQ=255)
                            tGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACCCCGCCCCGCCGGCCCAGCTCGGCAGAGCGagcagc                                                                                                         >  3:17709/1‑136 (MQ=255)
                            tGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGAAGCCACCGGGCGCGGCCCACCCCCCACACCCCCCACGCGGCCATGCCCGGCAGcgcggacac                                                                                                          >  5:28845/1‑130 (MQ=255)
                            tGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCCTGCCAGCAGCCCCCCCGCGCTCCCCACCCCCCACCCCCCCCACGCCGCCAACCTCGGCAGAGCGAACac                                                                                                          >  5:24483/1‑134 (MQ=255)
                              cAGCCCTTGCCGATATCAACAAAACCCCCGCAAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGc                                                                                                         <  8:312842/134‑1 (MQ=255)
                                 cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGGCGCTCCCCCCCCCCCACACCGCCCCGCCGGCCCTGCTCGGCAGCGCGATCACCAAGaa                                                                                                    >  7:142177/1‑136 (MQ=255)
                                 cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGGCGCTCCCCCCCCCCAACACCCCCCCCGCGGCCATGCTCGGCAGAGCGATCAGCaatca                                                                                                    >  2:109654/1‑133 (MQ=255)
                                 cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCCGGCGCGCCCCCCCCCCCACCCCCCCCCGGCGGCCATGCGCGGCCGAGCGATCAGCAAGa                                                                                                     >  1:170895/1‑135 (MQ=255)
                                 cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCCGGCGCTCCCCCCCCCCAACACCCCCCCGGCGGCCAAGCTCGGCAGAGCGATCAGCAAGaa                                                                                                    >  2:72933/1‑136 (MQ=255)
                                 cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCCCAACCGCCCCGGTGCCTGCAGCCCCCGGGCGCGCCCCCCCCCCCACAACCCCCCCGCGGCCCTGCTCGGCCGAGCCATCAGcaacaa                                                                                                    >  1:33432/1‑136 (MQ=255)
                                 cccATGCCGATATCAACAAAACCCCCGCCAACACATGGTTGAACACCCGCAACCCCCCCGGAGCCTGCAGCCACCGGGCCCACCCCACCCCCCACACCCCCCACGCGGCCATTCGAGCCAGAACGATCACcaacaa                                                                                                    >  2:196324/1‑136 (MQ=255)
                                     tGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACCCCCCCCCCGCGGCCATGCTCGGCCGCGCGCTCAGCAAGAACAcc                                                                                                >  1:247847/1‑136 (MQ=255)
                                      gCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCTGCAGCCACCCGGCGCTCCCCACCCCCAACAACCCCCACGCGGCCAAGCTCGGCAGAGCGATCAACAACAAAACCa                                                                                               >  4:228256/1‑136 (MQ=255)
                                        cGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCCCCCCCCACCCCCCCCCCCCGGCCCTGCTCGGCAGAGCGCTCAGCAACAACACCacc                                                                                             >  8:327429/1‑134 (MQ=255)
                                         gATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCCGGGCGCGCCCCCCCCCCCACCCCCCCCACGCCGCCAAGCGCCGCCGCGCGCTCACCAAGAACACCaccc                                                                                            >  6:201863/1‑133 (MQ=255)
                                                  aacaCCCCCGCCAACACTGGGTTGAACACCAGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACAAGCGCCAGCTGcc                                                                                   <  3:39508/133‑1 (MQ=255)
                                                            ccAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCCCCCCCACCACCCCCCCCGCGGCCCTGCGCGGCAGTGCGAGCCCCCAGAACACCAGCCCCCCCAGCCCCACTGGCac                                                                         >  6:227495/1‑135 (MQ=255)
                                                            ccAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCAACCCCAACACCGCCCAGGCGGCCATGCTAGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCa                                                                          >  8:291448/1‑135 (MQ=255)
                                                                cacTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAacgac                                                                     <  6:126007/136‑1 (MQ=255)
                                                                  cTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACgg                                                                   <  6:275585/136‑1 (MQ=255)
                                                                  cTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACgg                                                                   <  6:364905/136‑1 (MQ=255)
                                                                  cTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACgg                                                                   <  2:231432/136‑1 (MQ=255)
                                                                  cTTGGTTGAACACCCGCAACCGCCCCGGGGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACgg                                                                   <  5:201863/136‑1 (MQ=255)
                                                                       ttGACCACCCGCACCCGCCCCGGTGCCGGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCgg                                                              <  5:200160/136‑1 (MQ=255)
                                                                       gtGACCACCCGCACCCGCCCCGGTGCCTGCAGCCCCCGGGCGCTCCCCCCCCCCAACCCCGCCCAGGCGGCCATGCTCGGCAGAGCTATCATCAATAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCgg                                                              <  6:23505/135‑1 (MQ=255)
                                                                        tGAACACCCGCACCCGCCCCGTTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGATCGATAATAAATAACACCAGCGCCAGCTGCCACACTGGCAACGACGGcgcgt                                                              <  6:19092/135‑2 (MQ=255)
                                                                             acCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAAt                                                         >  5:249566/1‑135 (MQ=255)
                                                                                 gcaccggCCCGGGTGCTGGCCGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAcccc                                                    <  1:190121/130‑1 (MQ=255)
                                                                                            ggTGCCTGCAGCCACCGGGCGCTCCCCCCCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGCGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGCCCACCGCCa                                         >  8:9983/1‑136 (MQ=255)
                                                                                               gCCTGCAGCCACCGGGCGCTCCCCACCCCCACCACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACgg                                      <  4:88527/136‑1 (MQ=255)
                                                                                               gCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACgg                                      >  7:83494/1‑136 (MQ=255)
                                                                                                     aGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCa                                <  1:161741/136‑1 (MQ=255)
                                                                                                     aGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCa                                <  7:251083/136‑1 (MQ=255)
                                                                                                     aGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCa                                <  4:17709/136‑1 (MQ=255)
                                                                                                             ggCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGACAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGAcc                        <  8:246374/136‑1 (MQ=255)
                                                                                                                 cTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTgg                    >  8:323647/1‑136 (MQ=255)
                                                                                                                    cccACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCTATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGt                 <  5:231512/136‑1 (MQ=255)
                                                                                                                              acacCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTg       <  8:187093/136‑1 (MQ=255)
                                                                                                                              acacCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTg       <  2:94600/136‑1 (MQ=255)
                                                                                                                              acacCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTg       <  2:1772/136‑1 (MQ=255)
                                                                                                                               cacCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTg       >  4:154957/1‑135 (MQ=255)
                                                                                                                                 ccGCCCAGGCGGCCATGCTCGGCAGAGAGATCAGCAAGAACACCAGCTCCAGCTGCCACACTGGCAACGACGTCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACAAGACCTTGGGGTTCACCACCTGCaa    >  6:337751/1‑136 (MQ=255)
                                                                                                                                   gCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCCGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACg  <  3:228070/136‑1 (MQ=255)
                                                                                                                                   gCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAAc   >  2:56509/1‑135 (MQ=255)
                                                                                                                                   gCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACg  <  3:17079/136‑1 (MQ=255)
                                                                                                                                   gCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACg  <  4:173234/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
TCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACCGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTGGGGTTCACCACCTGCAACG  >  ACB143/2769944‑2770210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑