| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,425,070 | 0 | G | C | 33.3% | 48.4 / 11.8 | 27 | intergenic (‑29/‑89) | CJ019_04200/CJ019_04201 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (18/0); new base C (0/9); total (18/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTG > ACB143/4424935‑4425191 | tgCGAGTCGAGCTGCGCAGTCGCCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAACAACAACCGGCCAGCATCAGCACCCCAGCCAACGCCAGAATCGCACGCATGTACCATCCTCGGGGTCGCAAAAaa‑gaag > 8:56326/1‑136 (MQ=255) gAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAACAA‑GAAGGCg > 5:212103/1‑136 (MQ=255) gAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCg > 5:207961/1‑136 (MQ=255) gTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGAc > 2:280828/1‑136 (MQ=255) aGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGActc > 2:29452/1‑134 (MQ=255) tCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGcc > 6:23608/1‑136 (MQ=255) cGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCg > 2:244384/1‑136 (MQ=255) gCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACACGc > 3:166423/1‑136 (MQ=255) ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAAcc > 8:37809/1‑135 (MQ=255) ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAAcc > 7:352363/1‑135 (MQ=255) ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAAcc > 4:335832/1‑135 (MQ=255) ccAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAAcc > 5:33140/1‑135 (MQ=255) caccgccAGAACCGCACCATTGTACCATCCGCGGGGTCCAAAAAAA‑GACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCca < 4:104349/130‑1 (MQ=255) cGGCATAACCGCACGCATGTACCCTCCTCGGGGTCCCAAAAAA‑GACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCcatca < 5:254611/136‑1 (MQ=255) aGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGa > 7:156821/1‑135 (MQ=255) agcgcccgcaTGTTCCATCGTGGGGGTCTAAAAAAA‑GAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACt < 5:66768/134‑1 (MQ=255) cgcccgaaTGTTCCACCCTCGGGGTCCAAAAACA‑GACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGc < 7:95151/136‑1 (MQ=255) cgcacgcaTGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTg > 4:65168/1‑135 (MQ=255) cgcacgcaTGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTg > 3:104349/1‑135 (MQ=255) cccacgcaTGTCCCATCGTCGGGTTCCAAAAAAA‑ACCCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGc < 2:147396/134‑1 (MQ=255) cccacgcaGGTTCCCTAGTCGGGGACGCAAAAAA‑CACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGc < 5:107037/134‑1 (MQ=255) aTGTTCCATCCTCGGGGTCGAAAAAAA‑TAACGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCt < 7:333708/136‑1 (MQ=255) tCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCAATCATGAAACTGCACTATCTGCTACTcg > 2:171206/1‑136 (MQ=255) ctgggggTCTAAAAAAA‑GACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc < 4:111919/133‑1 (MQ=255) ccGGGGGCCCAAAAAACGACCGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTcgcc < 3:166098/135‑1 (MQ=255) gTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtg > 7:220491/1‑136 (MQ=255) gTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtg > 7:289179/1‑136 (MQ=255) gTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCtg > 8:351727/1‑136 (MQ=255) | TGCGAGTCGAGCTGCGCAGTCGGCTTGCCGCCACGCGCCGTTTCCATGGTCGCAGAAGCACAACCGGCCAGCATCAGCACCGCAGCCAACGGCAGAATCGCACGCATGTTCCATCCTCGGGGTCGCAAAAAA‑GAAGGCGACTCTAGCACTGGCAGGCATCCCGAGTGAATTGCCGCGCACTTGCCGCTGTCGCAGATAAAACATGCCATCACGAGGAACCCATCATGAAACTGCACTATCTGCTACTCGCCGCCCTG > ACB143/4424935‑4425191 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |