Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,938,4900GT32.8% 80.5 / 10.2 59intergenic (+22/+757)yhhJ/CJ019_05558Inner membrane transport permease YhhJ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/17);  new base T (0/19);  total (22/37)
Fisher's exact test for biased strand distribution p-value = 9.66e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGATCCTCAGAGTCTTACAAG  >  ACB143/5938358‑5938624
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aGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGaa                                                                                                                                      >  5:114662/1‑135 (MQ=255)
aGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGaa                                                                                                                                      >  5:171636/1‑135 (MQ=255)
aGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGaa                                                                                                                                      >  4:153255/1‑135 (MQ=255)
     tGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTg                                                                                                                                 >  3:106363/1‑135 (MQ=255)
     tGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCAGAAAAttt                                                                                                                                 >  4:194008/1‑134 (MQ=255)
          ggTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAAc                                                                                                                           >  5:127191/1‑136 (MQ=255)
          ggTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAAc                                                                                                                           >  7:77246/1‑136 (MQ=255)
          ggTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAAc                                                                                                                           >  6:95483/1‑136 (MQ=255)
              ggCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGc                                                                                                                       <  6:235021/136‑1 (MQ=255)
              ggCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGc                                                                                                                       <  2:208888/136‑1 (MQ=255)
              ggCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGc                                                                                                                       <  7:193831/136‑1 (MQ=255)
                                tatCCTGGCGCTGACGTTTATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGcc                                                                                                     >  8:88232/1‑136 (MQ=255)
                                  tCCTGGCGTGGACGTTTATCGGCCGGGTGTTCTTCTGGGTGGCGCTGGCAGGGTTTCGGCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCTAAAATTGTAAAACTGCCACAAACACAATGGGCGCCtt                                                                                                   <  6:335005/136‑1 (MQ=255)
                                  tCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGccgt                                                                                                   >  3:316800/1‑134 (MQ=255)
                                  tCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGccgt                                                                                                   >  7:161925/1‑134 (MQ=255)
                                  tCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCtt                                                                                                   >  3:156174/1‑136 (MQ=255)
                                       gcgcTGACGTTTATGGGCGTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTGGGGGGGAGCCGGGGGTCTTGAAGGCATGCGCTAGCGTCCGCCTAAAATTGCAAAACTGGCACAAAGACAACGGGCGCCTTTCGgc                                                                                              <  8:326925/136‑1 (MQ=255)
                                        cgcTGACGTTGATCGGCCTGGTGTTCTTCAGGGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCTAAAATTGGAAAACTGCCACAAAGACAAAGGGCGCCTTTCGgcg                                                                                             <  7:113010/136‑1 (MQ=255)
                                        cgcTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGAACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCTAAAATTGTAAAACTGGCACAAAGACAAAGGGCGCCTTTCGgcg                                                                                             <  1:123236/136‑1 (MQ=255)
                                                ttGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCTCCTTTCGGCGCCCTACt                                                                                      >  6:240346/1‑135 (MQ=255)
                                                ttGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACt                                                                                      >  8:87926/1‑135 (MQ=255)
                                                ttGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCATTCGGCGGCCTAATc                                                                                     >  7:339582/1‑136 (MQ=255)
                                                                   tccgCGGGGCGCGGGCACGGTTTCGCGGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCTAAAATTGTAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGt                                                                   <  1:328928/132‑1 (MQ=255)
                                                                   tcAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGt                                                                   <  4:156174/135‑1 (MQ=255)
                                                                    ccgCGGGGCGCGGGCACGGGTTCGTCGGAGCCTGGCGTCTTGAAGGCAGGCGCGAGCGTCCGCCGAAAATTGTAAAACTGGCACAAAGCAAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGt                                                                   <  3:55260/132‑1 (MQ=255)
                                                                    cAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGt                                                                   <  5:173857/134‑1 (MQ=255)
                                                                    cAGCGGGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGACGGCATGCGCGAGCGGCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGt                                                                   <  4:278872/134‑1 (MQ=255)
                                                                     aGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTGGGCGCCCGACTCACAGCCAGGGGGACTTGGCa                                                                 >  5:210484/1‑135 (MQ=255)
                                                                     aGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGtga                                                                 >  5:18433/1‑133 (MQ=255)
                                                                     aGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACCCACAGACAGGTCGACTTGTCAt                                                                >  6:135585/1‑136 (MQ=255)
                                                                       cggggCGGTGGCAGGGTTTCGTCGGAGCCGGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAACCACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATc                                                               <  4:70320/132‑1 (MQ=255)
                                                                            cgcTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCata                                                         >  7:21827/1‑136 (MQ=255)
                                                                              cTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACACACAGACAGGTCGACTTGTCATCGCCAtata                                                       >  7:10593/1‑136 (MQ=255)
                                                                                 gCAGGGTTTCGGCGGCGCCTGGCGGCTTGAAGGCTTGCGGGAGGGCCCGCCTAAAATTGCAAACCTGGCACAAAGACAAAGGGCGCCTTGCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCt                                                    <  5:339012/136‑1 (MQ=255)
                                                                                 gCACGGTTTCGGCGGAGCCTGGCGTCTTGAAGGCTTGCGCGAGCGGCCGCCGAAAATTGGAAAACGGGCACAATGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCt                                                    <  3:112675/136‑1 (MQ=255)
                                                                                 gCACGGTTTCGGCGGAGCCGGGCGTCTTGAAGGATTGCGCGAGCGTCCGCCTAAAATTGTAAAACTGGCACAAAGACAAAGGGCGCCTTGCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCt                                                    <  5:248752/136‑1 (MQ=255)
                                                                                    cGGTTTCGTCGGAGCCTGGCGTCTTTAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAATACAAAGGGCGCATTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGcc                                                 <  7:338688/136‑1 (MQ=255)
                                                                                    cGGTTTCGTCGGAGCCTGGCGGCTTGACGGCATGCGCGAGCGTCCGCCGAAAATTGTAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGcc                                                 <  1:68125/136‑1 (MQ=255)
                                                                                    cGGTTTCGCCGGCGCCTGGCGGCTTGAAGGCATGCGCGAGCGGCCGCCGAAAATTGTAAAACTCGCACAAACACAACGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGcc                                                 <  8:156702/136‑1 (MQ=255)
                                                                                     ggTTTCGGCGTAGCCTGGCGTATTGAAGGCATGCGCGAGCGTCCGACTAAAATTTTAAAACTGGCACAAACAAAACGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGccc                                                <  4:106363/136‑1 (MQ=255)
                                                                                      ttttCGTCGGTGCCTGGCGTGTTGAAGGCATGCGCGAGCGGCCGCCGAAAATTGCAAAACTGGCACAAAGACAAAGGGCGCCTTGCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCg                                               <  1:276692/135‑1 (MQ=255)
                                                                                      gTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCg                                               <  2:219170/136‑1 (MQ=255)
                                                                                               gAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCTAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTaa                                      <  6:124997/136‑1 (MQ=255)
                                                                                                    tGGGGTCTTGAAGGCATGCGCGAGCGTCCGCCTAAAATTGTAAAACTGCCACAAACACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCt                                 <  1:262436/136‑1 (MQ=255)
                                                                                                    tGGCTTGTTGAAGGCATGCGCGAGCGTCCGCCTAAATTTGTAAAACTGGCACAAAGACAACGGGCGCCTTGCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCt                                 <  4:94824/136‑1 (MQ=255)
                                                                                                    gggCGTCTGGCAGGAATGCGCGCGCGTCCGCCTAAAATGGGAAACCTGGCACAAAGACAACGGGCGCCTTGGGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCt                                 <  2:75164/135‑1 (MQ=255)
                                                                                                      gCGTCTTGAAGGCATGCGCGAGCGTCCGCCTAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCt                                 <  3:108195/134‑1 (MQ=255)
                                                                                                             gAAGGCATGCGCGAGCGTCCGCCTAAAATTGGAAAACTGGCACAAACACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAAc                         <  7:29849/135‑1 (MQ=255)
                                                                                                              acggCTTGCGCGAGGGGCCGCAAAAAATTGTAAACCTGGCAAATCCACAACGGGCGGCTTTGGGGCCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAAcc                        <  5:195584/133‑1 (MQ=255)
                                                                                                              aaGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCa                       >  6:360131/1‑136 (MQ=255)
                                                                                                                  tagcggcgAGCGGCCCCCTAAATTTGTAAACCGGGCACAAACACATCGGCGGCCTTGCGGCCCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGAt                   <  1:188550/131‑1 (MQ=255)
                                                                                                                  cATGCGCGAGCGTCCGCCTAAAATTGTAAACCTGGCACAAATACAAAGGGCGCCTTTGGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGAt                   <  4:59763/136‑1 (MQ=255)
                                                                                                                  cATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAACGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGAt                   <  8:77246/136‑1 (MQ=255)
                                                                                                                      cgcgAGCGTCCGCCTAAAATTGGAAAACTGCCAAAAACAAAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGATCCTc               <  6:18433/136‑1 (MQ=255)
                                                                                                                      cgcgAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGATCCt                >  3:109018/1‑135 (MQ=255)
                                                                                                                       gcgAGCGTCCGCCTAAAATTGGAAAACTGGCACAAAGACAACGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGATCCTCa              <  5:138277/136‑1 (MQ=255)
                                                                                                                        cgAGCGTCCGCCTAAATTTGGAAAACTGCCACAAACACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGATCCTCag             <  5:102083/136‑1 (MQ=255)
                                                                                                                        cgAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGATCCTCag             <  7:185805/136‑1 (MQ=255)
                                                                                                                              tCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCATTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGATCCTCAGAGTCtt       <  1:280552/136‑1 (MQ=255)
                                                                                                                                   cgAAAATTGGAAAACTGGCACAAAGACACCGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGATCCTCAGAGTCTTACAAg  <  8:246369/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
AGGGATGCCGGGTGGGCGGTGGTATGGCCGGATATCCTGGCGCTGACGTTGATCGGCCTGGTGTTCTTCAGCGTGGCGCTGGCACGGTTTCGTCGGAGCCTGGCGTCTTGAAGGCATGCGCGAGCGTCCGCCGAAAATTGGAAAACTGGCACAAAGACAAAGGGCGCCTTTCGGCGCCCTACTCACAGACAGGTCGACTTGTCATCGCCATATACCTGCCCGGCTCTGTAATTGCTGGTCGAACCAGGATCCTCAGAGTCTTACAAG  >  ACB143/5938358‑5938624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑