Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,514	T→C	intergenic (‑523/+1128)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,514	0	T	C	100.0%	102.5 / NA	27	intergenic (‑523/+1128)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/20);  total (7/20)

CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑GGATC‑‑‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTC  >  ACB143/5386410‑5386608                                                                                                                     |                                                                                               cattttttATTGATAACAAAAATTTATCGAATTCTTTCATTTAAGCCCCTAATATTACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGc                                                                     <  6:94807/134‑1 (MQ=255)               aaCAAAAATTGATCGAATTCTTTCATTTAAGCCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAc                                                       >  3:238286/1‑136 (MQ=255)                  aaaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCtt                                                    >  3:54328/1‑136 (MQ=255)                    aaaTTGATCGAATTCTTTCATTTAAGACCCAAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc                                                  <  5:118011/136‑1 (MQ=255)                          aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                             <  7:239370/135‑1 (MQ=255)                          aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                             <  3:201945/135‑1 (MQ=255)                          aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGc                                            >  2:271702/1‑136 (MQ=255)                          aTCGAATTCTTTCATTAAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑‑‑AC‑GGTAC‑‑‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                             <  5:96353/135‑1 (MQ=255)                                       aTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                               <  6:94322/136‑1 (MQ=255)                                       aTTTAAGACCCTAATATGACATCTCGGCCGGCTCGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                               <  2:350790/136‑1 (MQ=255)                                             gccccTAATATGACCTCTCGGCCGGCTGGTCGACGACGGCGGTCTCAGG‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCggg                         <  2:343379/134‑1 (MQ=255)                                              aCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt                        <  8:204064/136‑1 (MQ=255)                                              aCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt                        <  3:101412/136‑1 (MQ=255)                                              aCCCTAACATGACCTCTCGGCCGGCTGGGCCACGCCGGCGGTCTCAGT‑‑GGTGTACG‑GGACA‑‑‑‑AC‑CCCCGACGCTAGCACCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt                        <  2:262704/136‑1 (MQ=255)                                                    atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg                  <  3:25626/136‑1 (MQ=255)                                                    atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg                  <  4:264170/136‑1 (MQ=255)                                                    atatGACATCTCGGCCGGCTTGTCGACGACGGCGGGCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg                  <  7:102261/136‑1 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                >  2:228278/1‑105 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                                <  1:228278/105‑1 (MQ=255)                                                     tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAggg                 >  1:106937/1‑136 (MQ=255)                                                             ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCCGGTGCCAGGGCGTGCCCt         >  2:192374/1‑136 (MQ=255)                                                                ggccggcTTGTCGACGACGGCGGTCTCCGT‑‑GGTG‑‑TACGTAACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGccc          <  4:63609/132‑1 (MQ=255)                                                                ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TA‑CGGTCCAA‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt        <  2:323814/134‑1 (MQ=255)                                                                ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGT‑‑‑GT‑ACGTCACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt        <  2:257268/134‑1 (MQ=255)                                                                ggccggcTTGGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGccc          <  2:276696/132‑1 (MQ=255)                                                                   cggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCt   <  1:10288/136‑1 (MQ=255)                                                                    ggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTc  >  6:269845/1‑136 (MQ=255)                                                                                                                     |                                                                                               CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑GGATC‑‑‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTC  >  ACB143/5386410‑5386608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑