Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 4,759,606 G→A A28T (GCC→ACC)  ligM_KToptimized → sequence generated by OPTIMIZER for KT2440

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,759,6060GA99.4% 616.4 / NA 176A28T (GCC→ACC) ligM_KToptimizedsequence generated by OPTIMIZER for KT2440
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (103/71);  minor base C (1/0);  total (105/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

ATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTAAAGACG  >  ACB143/4759473‑4759735
                                                                                                                                     |                                                                                                                                 
aTTGTGAGAGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCAcc                                                                                                                                 <  8:266904/136‑1 (MQ=255)
   gtgAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTa                                                                                                                               <  2:123527/135‑1 (MQ=255)
    tgAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGCCCGCCGGCGGCAACACCGTGGAAATGTTGCGTCACTCCCAGATTGGCACCTACg                                                                                                                             >  2:178338/1‑136 (MQ=255)
    tgAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGAATAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTAc                                                                                                                              <  1:290495/135‑1 (MQ=255)
     gAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGt                                                                                                                            >  6:95806/1‑136 (MQ=255)
       gCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTc                                                                                                                           >  4:73467/1‑135 (MQ=255)
         ggATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTAc                                                                                                                        >  3:268271/1‑136 (MQ=255)
          gATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTAcc                                                                                                                       >  3:17375/1‑136 (MQ=255)
              aCAATTTCACACCAACACATAGGGTGTAGGAGGTCTACATGAGCGCCCCGACTAACCTGGAGCAGGTGCGGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGgt                                                                                                                   <  2:287433/136‑1 (MQ=255)
               cAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGgtc                                                                                                                  >  8:270983/1‑136 (MQ=255)
                       cacCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCgcgc                                                                                                           >  1:65008/1‑135 (MQ=255)
                        acCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCgcgc                                                                                                           >  7:271810/1‑134 (MQ=255)
                        acCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCgcgc                                                                                                           >  4:87271/1‑134 (MQ=255)
                              acaTAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTtc                                                                                                   <  3:36122/136‑1 (MQ=255)
                                   gggTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa                                                                                              <  7:87893/136‑1 (MQ=255)
                                    ggTTTAGGAGGTATACATGAGCGCCCCGACTACCCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa                                                                                              <  3:33054/135‑1 (MQ=255)
                                    ggTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa                                                                                              <  8:161473/135‑1 (MQ=255)
                                     gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACt                                                                                            >  7:274666/1‑136 (MQ=255)
                                     gTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACt                                                                                            <  2:370101/136‑1 (MQ=255)
                                       ttAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTgg                                                                                          >  8:111393/1‑136 (MQ=255)
                                         aggaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGc                                                                                         <  5:87260/135‑1 (MQ=255)
                                         aggaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGc                                                                                         <  4:136620/135‑1 (MQ=255)
                                         aggaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCg                                                                                        >  6:263901/1‑136 (MQ=255)
                                         aggaggTATACATGAGAGCCCCGACTAACCTGCTGCAGGTGCTGGCCGCCGGCTGCAACAACGTTGACATGTTGCGTAACTCCCAGTTTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGc                                                                                         <  4:196982/135‑1 (MQ=255)
                                           gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTaa                                                                                              <  6:336240/128‑1 (MQ=255)
                                           gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGt                                                                                       >  1:351795/1‑135 (MQ=255)
                                           gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGt                                                                                       >  3:158132/1‑135 (MQ=255)
                                           gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa                                                                                      >  4:275107/1‑136 (MQ=255)
                                           gaggTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTa                                                                                      >  6:168153/1‑136 (MQ=255)
                                                 taCCTGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                                 <  2:6828/135‑1 (MQ=255)
                                                 taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                                 >  5:6079/1‑135 (MQ=255)
                                                 taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                                 >  6:185414/1‑135 (MQ=255)
                                                 taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                                 >  4:243300/1‑135 (MQ=255)
                                                 taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                                 >  7:187340/1‑135 (MQ=255)
                                                 taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                                 >  1:325892/1‑135 (MQ=255)
                                                 taCATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGaa                                                                                 >  5:165459/1‑135 (MQ=255)
                                                   cATGAGCGCCCCTACTACCCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAg                                                                              >  6:297595/1‑136 (MQ=255)
                                                   cATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACAGGCGTACCGAACAg                                                                              >  5:39523/1‑136 (MQ=255)
                                                         cgcCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCt                                                                        >  6:55379/1‑136 (MQ=255)
                                                         cgcCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCt                                                                        >  6:222354/1‑136 (MQ=255)
                                                          gcCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCtt                                                                       >  3:139841/1‑136 (MQ=255)
                                                           ccccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTg                                                                      >  4:18144/1‑136 (MQ=255)
                                                           ccccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTg                                                                      <  2:351795/136‑1 (MQ=255)
                                                            cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGGCAGAGTTCTCTAACTGGCGTTCCGAACAGTGGGCTTgg                                                                     >  3:8551/1‑136 (MQ=255)
                                                            cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg                                                                     >  8:305771/1‑136 (MQ=255)
                                                            cccGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTgg                                                                     >  4:257916/1‑136 (MQ=255)
                                                              cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGt                                                                                       <  4:245487/116‑1 (MQ=255)
                                                              cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGt                                                                                       >  3:245487/1‑116 (MQ=255)
                                                              cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGCACAGTGGGCTTGgcg                                                                   >  6:193759/1‑136 (MQ=255)
                                                              cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                                   <  1:157933/136‑1 (MQ=255)
                                                              cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                                   >  7:314950/1‑136 (MQ=255)
                                                              cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                                   >  5:192124/1‑136 (MQ=255)
                                                              cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                                   <  5:216538/136‑1 (MQ=255)
                                                              cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                                   >  6:152124/1‑136 (MQ=255)
                                                              cGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGgcg                                                                   <  1:23383/136‑1 (MQ=255)
                                                              cGACTAACCTGGAGCAGCTGATGGCCCCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCACAGTTCAATAACTGGCGTACCGAACAGTTGGCTTGgcg                                                                   >  2:35510/1‑136 (MQ=255)
                                                                aCTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCa                                                                 <  2:297541/136‑1 (MQ=255)
                                                                 cTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCaa                                                                >  1:43815/1‑136 (MQ=255)
                                                                 cTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCaa                                                                >  2:22719/1‑136 (MQ=255)
                                                                   aaCCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACt                                                              <  7:126460/136‑1 (MQ=255)
                                                                      cTGGCGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTccg                                                           <  8:76918/136‑1 (MQ=255)
                                                                         gAGCAGGTGCTGGCCACCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCAGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTccgcc                                                         <  6:214812/135‑1 (MQ=255)
                                                                             cggggCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCt                                                    <  1:24857/132‑1 (MQ=255)
                                                                             aGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCt                                                    <  1:144998/136‑1 (MQ=255)
                                                                                 gCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACAGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTc                                                >  6:154834/1‑136 (MQ=255)
                                                                                 gCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTATAAATGGCGTACCGAACAGTGGGCTTGGCGCAACACCGCCGTGCAGTTc                                                >  3:193781/1‑136 (MQ=255)
                                                                                  cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCCGAGTTCTATAACTGGCGTACCGCACCGTGGGCTTGGCGCAACTCCGCCGTGCGGGTCg                                               >  7:298686/1‑136 (MQ=255)
                                                                                  cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTc                                                >  5:286350/1‑135 (MQ=255)
                                                                                  cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCg                                               >  1:235998/1‑136 (MQ=255)
                                                                                  cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGGTc                                                >  2:346910/1‑135 (MQ=255)
                                                                                  cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGGTCg                                               >  2:262830/1‑136 (MQ=255)
                                                                                  cTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACAGGCGTACCGAACAGTGGGCTAGGCGCAACTACGCCGTGCAGTTCg                                               >  5:298528/1‑136 (MQ=255)
                                                                                     gccgccGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGAcc                                            <  7:208567/136‑1 (MQ=255)
                                                                                      ccgccgGCGTCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCa                                           <  8:228813/136‑1 (MQ=255)
                                                                                      ccgccgGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGTCGTACCGAAGAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCa                                           <  4:352139/136‑1 (MQ=255)
                                                                                       cgccgGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCaa                                          <  6:28693/136‑1 (MQ=255)
                                                                                       cgccgGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCa                                           >  1:179194/1‑135 (MQ=255)
                                                                                            gcggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCACCTCCGCCGTGCTGTTCGACCAAACcca                                     >  6:354287/1‑136 (MQ=255)
                                                                                            gcggcAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACcca                                     >  7:113276/1‑136 (MQ=255)
                                                                                                 aaCACCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACcaca                                <  3:69679/136‑1 (MQ=255)
                                                                                                    acCGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACAtgg                             <  2:339490/136‑1 (MQ=255)
                                                                                                      cGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACAtggtg                           <  7:111393/136‑1 (MQ=255)
                                                                                                      cGTTGAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACAtggtg                           <  4:268271/136‑1 (MQ=255)
                                                                                                          gAAATGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTgg                                                                           >  2:151496/1‑84 (MQ=255)
                                                                                                           aaaTGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCCCATGGTGGACCt                      >  8:134101/1‑136 (MQ=255)
                                                                                                           aaaTGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCt                      >  6:114219/1‑136 (MQ=255)
                                                                                                           aaaTGTTGCGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGATCTATAACTGGCGTACCGAACAGTGGGCTTGGCGCAACACCGCCGTGCTGTACGACCAAACCCACCACATGGTGGACCt                      >  5:218098/1‑136 (MQ=255)
                                                                                                           aaaTGTTGCGTAACTCCCAGATTGGCACATACGTCTACCCGGTCGTCGCGCCAGAGTTATATAAATGGAGAACCGAACACTGGGCATGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACAACATGGTGGACCt                      >  5:261547/1‑136 (MQ=255)
                                                                                                                  gCGTAACTCCCATTTTGGCACCTACGTCTACCCGGTCGGCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATc               <  8:298686/136‑1 (MQ=255)
                                                                                                                   cGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATcc              >  5:355170/1‑136 (MQ=255)
                                                                                                                   cGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATc               <  1:280809/135‑1 (MQ=255)
                                                                                                                   cGTAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGAAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATc               <  5:304843/135‑1 (MQ=255)
                                                                                                                     tAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCcgc            >  1:86994/1‑136 (MQ=255)
                                                                                                                     tAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGAGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCcgc            >  7:348802/1‑136 (MQ=255)
                                                                                                                     tAACTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACAGAACAGTGGGCTTGGCGCAACTCAGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCcgc            >  2:282245/1‑136 (MQ=255)
                                                                                                                      aaCTCCCAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCcgcg           >  7:266148/1‑136 (MQ=255)
                                                                                                                           ccAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTaa       <  4:87914/135‑1 (MQ=255)
                                                                                                                           ccAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTaa       <  3:25477/135‑1 (MQ=255)
                                                                                                                           ccAGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTaa       <  1:146605/135‑1 (MQ=255)
                                                                                                                             aGATTGGCACCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTAAAGa    <  1:307198/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                 
ATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCCAGAGTTCTCTAACTGGCGTACCGAACAGTGGGCTTGGCGCAACTCCGCCGTGCTGTTCGACCAAACCCACCACATGGTGGACCTGTACATCCGCGGTAAAGACG  >  ACB143/4759473‑4759735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑