| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,510 | 2 bp→TA | intergenic (‑519/+1131) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,510 | 0 | A | T | 92.0% | 48.1 / ‑5.2 | 27 | intergenic (‑519/+1132) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/2); major base T (7/16); minor base G (0/2); total (7/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
| * | ACB143 | 5,386,511 | 0 | T | A | 92.3% | 73.1 / ‑6.7 | 27 | intergenic (‑520/+1131) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base A (7/17); total (7/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑GGATC‑‑‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTC > ACB143/5386410‑5386608 || cattttttATTGATAACAAAAATTTATCGAATTCTTTCATTTAAGCCCCTAATATTACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGc < 6:94807/134‑1 (MQ=255) aaCAAAAATTGATCGAATTCTTTCATTTAAGCCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAc > 3:238286/1‑136 (MQ=255) aaaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCtt > 3:54328/1‑136 (MQ=255) aaaTTGATCGAATTCTTTCATTTAAGACCCAAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTcc < 5:118011/136‑1 (MQ=255) aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg < 7:239370/135‑1 (MQ=255) aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg < 3:201945/135‑1 (MQ=255) aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGc > 2:271702/1‑136 (MQ=255) aTCGAATTCTTTCATTAAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑‑‑AC‑GGTAC‑‑‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg < 5:96353/135‑1 (MQ=255) aTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 6:94322/136‑1 (MQ=255) aTTTAAGACCCTAATATGACATCTCGGCCGGCTCGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 2:350790/136‑1 (MQ=255) gccccTAATATGACCTCTCGGCCGGCTGGTCGACGACGGCGGTCTCAGG‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCggg < 2:343379/134‑1 (MQ=255) aCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt < 8:204064/136‑1 (MQ=255) aCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt < 3:101412/136‑1 (MQ=255) aCCCTAACATGACCTCTCGGCCGGCTGGGCCACGCCGGCGGTCTCAGT‑‑GGTGTACG‑GGACA‑‑‑‑AC‑CCCCGACGCTAGCACCGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt < 2:262704/136‑1 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 3:25626/136‑1 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 4:264170/136‑1 (MQ=255) atatGACATCTCGGCCGGCTTGTCGACGACGGCGGGCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 7:102261/136‑1 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg > 2:228278/1‑105 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 1:228278/105‑1 (MQ=255) tatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAggg > 1:106937/1‑136 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCCGGTGCCAGGGCGTGCCCt > 2:192374/1‑136 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCCGT‑‑GGTG‑‑TACGTAACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGccc < 4:63609/132‑1 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TA‑CGGTCCAA‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 2:323814/134‑1 (MQ=255) ggccggcTTGTCGACGACGGCGGTCTCAGT‑‑GGT‑‑‑GT‑ACGTCACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt < 2:257268/134‑1 (MQ=255) ggccggcTTGGCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGccc < 2:276696/132‑1 (MQ=255) cggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCt < 1:10288/136‑1 (MQ=255) ggcTTGTCGACGACGGCGGTCTCAGT‑‑GGTG‑‑TACGGTACAA‑‑A‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTc > 6:269845/1‑136 (MQ=255) || CCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑‑‑CA‑GGATC‑‑‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGGCTC > ACB143/5386410‑5386608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |