breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 27 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 1,188,721 | 0 | A | C | 56.7% | 9.9 / 24.6 | 34 | Q52P (CAG→CCG) | CJ019_01117 | hypothetical protein |
* | ACB143 | 1,188,694 | 0 | T | G | 56.5% | ‑3.1 / 10.8 | 26 | L43R (CTG→CGG) | CJ019_01117 | hypothetical protein |
* | ACB143 | 1,537,460 | 0 | T | G | 56.2% | ‑2.7 / 11.8 | 16 | V403G (GTG→GGG) | mdfA | Multidrug transporter MdfA |
* | ACB143 | 3,440,331 | 0 | T | G | 56.2% | ‑1.5 / 16.1 | 35 | A19A (GCT→GCG) | CJ019_03266 | hypothetical protein |
* | ACB143 | 4,927,185 | 0 | A | C | 52.8% | 4.7 / 20.6 | 36 | W194G (TGG→GGG) | srp54 | Signal recognition particle 54 kDa protein |
* | ACB143 | 994,235 | 0 | T | G | 52.0% | 6.3 / 19.2 | 25 | R54R (CGT→CGG) | CJ019_00938 | hypothetical protein |
* | ACB143 | 5,251,184 | 0 | C | A | 52.0% | 18.0 / 30.3 | 25 | intergenic (‑64/+80) | CJ019_04948/fabV | hypothetical protein/Enoyl‑[acyl‑carrier‑protein] reductase [NADH] |
* | ACB143 | 3,138,350 | 0 | T | G | 50.0% | 8.2 / 20.7 | 36 | F240V (TTT→GTT) | rbsC_2 | Ribose import permease protein RbsC |
* | ACB143 | 6,067,354 | 0 | A | C | 43.4% | 59.4 / 19.0 | 53 | V162G (GTG→GGG) | cheB_5 | Protein‑glutamatemethylesterase/protein‑glutamin e glutaminase |
* | ACB143 | 5,693,183 | 0 | T | G | 42.9% | 5.6 / 13.0 | 42 | R238R (CGT→CGG) | pyrC' | Dihydroorotase‑like protein |
* | ACB143 | 690,139 | 1 | . | G | 41.7% | ‑3.2 / 21.1 | 12 | intergenic (+16/‑2443) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
* | ACB143 | 4,245,128 | 0 | A | C | 40.7% | 28.4 / 10.0 | 60 | L178R (CTG→CGG) | CJ019_03982 | Crotonyl‑CoA hydratase |
* | ACB143 | 3,737,443 | 0 | T | C | 36.1% | 46.1 / 17.0 | 38 | I229I (ATT→ATC) | paoB | Aldehyde oxidoreductase FAD‑binding subunitPaoB |
* | ACB143 | 6,043,196 | 0 | C | A | 33.7% | 89.4 / 36.5 | 89 | A37S (GCC→TCC) | puuD_3 | Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD |
* | ACB143 | 1,113,758 | 0 | C | G | 33.3% | 83.1 / 10.8 | 50 | F49L (TTC→TTG) | lptF | Lipopolysaccharide export system permeaseprotein LptF |
* | ACB143 | 2,770,075 | 0 | G | C | 33.3% | 86.6 / 11.0 | 51 | A157G (GCG→GGG) | CJ019_02613 | hypothetical protein |
* | ACB143 | 4,425,070 | 0 | G | C | 33.3% | 48.4 / 11.8 | 27 | intergenic (‑29/‑89) | CJ019_04200/CJ019_04201 | hypothetical protein/hypothetical protein |
* | ACB143 | 5,938,490 | 0 | G | T | 32.8% | 80.5 / 10.2 | 59 | intergenic (+22/+757) | yhhJ/CJ019_05558 | Inner membrane transport permease YhhJ/hypothetical protein |
* | ACB143 | 3,273,878 | 0 | C | A | 30.9% | 62.7 / 13.6 | 42 | D40E (GAC→GAA) | fabG_5 | 3‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG |
* | ACB143 | 303,994 | 0 | G | T | 30.2% | 70.3 / 12.4 | 53 | G477G (GGC→GGA) | ydeP_1 | Protein YdeP |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | ACB143 | 1731043 = | 73 (1.220) | 12 (0.230) | 10/228 | 2.9 | 17.2% | coding (110/426 nt) | spxA | Regulatory protein Spx |
? | ACB143 | = 1770457 | 52 (1.000) | intergenic (+135/+157) | CJ019_01684/higA‑1 | hypothetical protein/Antitoxin HigA‑1 |