breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 27 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,188,7210AC56.7% 9.9 / 24.6 34Q52P (CAG→CCG) CJ019_01117hypothetical protein
*ACB1431,188,6940TG56.5% ‑3.1 / 10.8 26L43R (CTG→CGG) CJ019_01117hypothetical protein
*ACB1431,537,4600TG56.2% ‑2.7 / 11.8 16V403G (GTG→GGG) mdfAMultidrug transporter MdfA
*ACB1433,440,3310TG56.2% ‑1.5 / 16.1 35A19A (GCT→GCGCJ019_03266hypothetical protein
*ACB1434,927,1850AC52.8% 4.7 / 20.6 36W194G (TGG→GGG) srp54Signal recognition particle 54 kDa protein
*ACB143994,2350TG52.0% 6.3 / 19.2 25R54R (CGT→CGGCJ019_00938hypothetical protein
*ACB1435,251,1840CA52.0% 18.0 / 30.3 25intergenic (‑64/+80)CJ019_04948/fabVhypothetical protein/Enoyl‑[acyl‑carrier‑protein] reductase [NADH]
*ACB1433,138,3500TG50.0% 8.2 / 20.7 36F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
*ACB1436,067,3540AC43.4% 59.4 / 19.0 53V162G (GTG→GGG) cheB_5Protein‑glutamatemethylesterase/protein‑glutamin e glutaminase
*ACB1435,693,1830TG42.9% 5.6 / 13.0 42R238R (CGT→CGGpyrC'Dihydroorotase‑like protein
*ACB143690,1391.G41.7% ‑3.2 / 21.1 12intergenic (+16/‑2443)bauC/PP_RS08750Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val
*ACB1434,245,1280AC40.7% 28.4 / 10.0 60L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
*ACB1433,737,4430TC36.1% 46.1 / 17.0 38I229I (ATT→ATCpaoBAldehyde oxidoreductase FAD‑binding subunitPaoB
*ACB1436,043,1960CA33.7% 89.4 / 36.5 89A37S (GCC→TCC) puuD_3Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD
*ACB1431,113,7580CG33.3% 83.1 / 10.8 50F49L (TTC→TTGlptFLipopolysaccharide export system permeaseprotein LptF
*ACB1432,770,0750GC33.3% 86.6 / 11.0 51A157G (GCG→GGG) CJ019_02613hypothetical protein
*ACB1434,425,0700GC33.3% 48.4 / 11.8 27intergenic (‑29/‑89)CJ019_04200/CJ019_04201hypothetical protein/hypothetical protein
*ACB1435,938,4900GT32.8% 80.5 / 10.2 59intergenic (+22/+757)yhhJ/CJ019_05558Inner membrane transport permease YhhJ/hypothetical protein
*ACB1433,273,8780CA30.9% 62.7 / 13.6 42D40E (GAC→GAAfabG_53‑oxoacyl‑[acyl‑carrier‑protein] reductase FabG
*ACB143303,9940GT30.2% 70.3 / 12.4 53G477G (GGC→GGAydeP_1Protein YdeP

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB143 1731043 =73 (1.220)12 (0.230) 10/228 2.9 17.2% coding (110/426 nt) spxA Regulatory protein Spx
?ACB143 = 1770457 52 (1.000)intergenic (+135/+157) CJ019_01684/higA‑1 hypothetical protein/Antitoxin HigA‑1